pycom13g12490

calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001204
Physical Location & Seq
Forward (+)
8715767 .. 8723321
7555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g12490.2

Sequence Viewer

Length: 3081 bp
ATGGCTGATACCAGGAGATACCCCCCCAATCAGCATCTAGACCTTGCCCAGATATTGCAAGAAGCAAAGAACCGTTGGCTTCGTCCAGCTGAAATTTGTGAAATACTTCGCAACTATCGGCATTTTGAATTGACCACAGATCCACCCGTCAGGCCTCCAGCTGGTTCTTTGTACCTGTTTGATCGAAAAGCACTTCGATATTTTCGTAAAGACGGTCATCGCTGGAGGAAGAAAAAGGATGGAAAGACTGTAAAAGAAGCCCATGAAAAGTTGAAGGCTGGCAGTGTAGACGTACTTCATTGTTACTATGCCCATGGGGAGGACAATGCAAATTTTCAACGGCGGAGTTATTGGATGCTTGACACGCATTTACAGCACATTGTTCTTGTGCATTACAGAAATGTTGAAGAGGCAAACTCAGCTGCCCGTATGGTTCAAACATCCTTTGGCTCAAGTCCACTTCAAACATCCTTTGCTTCAAGTCCAGTTCATACATCCTTTGCTTCAAGTCCAAACAGAGTTGACTTGGGTGGACAAACAGAATTTGAGGATGTGGATTCTCGGAGAGATGCAGGAACTTCATCTGTTGAGCAACCGATGTTTGGTTCCTTTTTTCATAATGCTTCTTTGCTTCCTCAACAAGTTGGAGGATTTCCTGAGTCATTCAGGGATCCCTCTAGTACATGGTATGGTGGACCTAAATTTGATCATGGTGCTGGATTATCTGCCTGGCAAGGAATGGATAGCTCAACGAGAAATGAGCACATCATGCATGGCCAAAACCTTTTTGTTGAAGAACCTAACAGAGCTGACTTTATAACTCTTAAGCTAACAGATGCTAAAGTGGATGCTCGTAGTGGAGTCAAAGATGTAACTTGTGAAGATAGGTTGACCACTAACATAGATGTCCAAATTGTCTCAGCACCTTCTCAAAGAGAAGATCAGGTGCCAAAGGAGCATGACTTCAATGTGCTTCATCCTGAGGTTCAAGATTATTCTGATCCTCAGATTGTAATAACTCCTTCCAACCAAGTTGAAGAGAATAGAGATGGTGGTGTGCGTAATGAACCAGTGGAACTGAAGAAACTAGACAGCTTTGGGAGATGGATGGATAAAGAAATTGGTGTGGATTGTGATGATTCCTTGATGGCTTCTGATTCTGGTAATTACTGGAGTACATTTGATGCTGAGAGTGGTGATAAGGAAGTGTCCAGTTTATCACACCATATGCAGTTGGATATAGAGTCACTGGGTCCTTCTCTTTCCCAAGAACAACTATTTACTATTTCTGATTTTTCACCAGATTGGGCTTATTCAGGAACTGAAACAAAGGTTTTAATAGTTGGCAGTTTTCTGGGAAGCAAGAAGGATTGTGCTGACACTAACTGGGGATGCATGTTTGGTGAAATAGAAGTTTCTGCTGAAATTCTATCTAACAATGCCATTAGATGTCAAACTCCTCTGCATGCTCCCGGGCGTGTTCCATTCTATGTGACCGGCAGAAATAGGTTAGCCTGCAGTGAAATTAGGGAGTTTGAATACCGTGAAAAACCCATTGGAATCGCTATCAACAGGTTAAGAGATGATGAATTACGCTTTCAGATACGACTAGCAAAATTGTTGAGCTTGGGCTCAGAGAGGAAGTGGTTGATATGTGCTGTTCCAGATTGTGATAAATGTAAGCTCAAAAGTTCCATATTTTCAATGCGAAGCAACAGTGAAAGTGATTGGGTAACAATTGATGGGGCTTCTGTGGCATGCGAAAGTGATCACTTGAGCCGTAGGGATGTCTTGATTCAGAATTTGTTGAAGGATAGACTCTGTGAATGGCTAGTCTGTAAAATTCATGAAGGAGGCAAAGGACTACATGTTCTGGATAATGAAGGCCAAGGTGTTCTACATTTGACGGGTGCTCTTGGTTACGAGTGGGCCATAGGTCCCATAATTGCTGCAGGTGTCAATCCCAATTTCAGAGATGCTCACGGAAGAACAGGGCTTCACTGGGCATCATATTTTGGAAGAGAGGAAACTGTCATTGCGTTGCTTCGATTGGGTGCCGCTCCAGGAGCCGTTGAGGACCCAACATCTGTGTTTCCTGGCGGACAAACTGCTGCTGATCTAGCCTCGAGTAGAGGGCATAAAGGGATTGCTGGATATTTGGCAGAAGCGGATTTAACCAGTCACCTAGAGTTGTTGACCATGAATGAGAATATAATGAACAATGCTACCGCAACTATTGCAGCTGAGAAGGCCAAACAGACTGCAGAAGTTGCTGAATCTGATGTTGCAGTAGATGAGCAACATTTTCTCAAAAACCCTATAGCAGCTGTTAGGAAATCAGCTCATGCGGCTGCTCTAATTCAAGAGTCTTTCCGGGCTCGTTCATTTCGTCAGAGACAGTTAACCAAGAGCAGAGCAGATATTTCTGAAGTTCAGTCTCAGGACCTTGTTGCACGTCGTTCCTTGAAGAGAGTCCAGAAGTTTGCTCACTATGAGGATTATCTGCATGTCGCAGCAGCTCTGAAGATCCAACAAAACTATCGTGGATGGAAGGGAAGAAAGGATTATCTGAAGATTCGTGACCGTATTGTTAAAATTCAGGCTCATGTGAGGGGACATCAGGTTCGGAAGAATTACAAAAAGGTTTTGTGGTCTGTTGGTATACTGGAAAAGGTTATACTGCGTTGGAGGCGAAAAGGAACTGGTTTGCGAGGATTTCGGGTGGAAAAGCCTACTGAAGATGCATCTTCAGAGGTTAAGAAAAATGATGACTATGAATTTCTTAGTGTCGGTCGGAAGCAGAAATTTGCTGGGGTTGAGAAAGCTCTCGCAAGGGTACGGTCCATGGCTCGTCAGCCTGAAGCGCGCGAACAATACATGAGGCTACTTTCAAACTTTGAGAAACTTGAGATTGCGGACGGGGAGAACCAGGCCTCGAATCAAAATGAAAGCTCCGACGAAAAAGTTATAGATGAAGTTCTGCGTGCACTCAATGAAGGAAGGACGGGAGAAATGGGAAAAAGCAGAATTGTATATGGCTCAGATTTCCTTTTTCCTTACTTAACCAGTAGTTTTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 CG-1 IQ TIG

Protein Analysis

1027

Amino Acids

115.55

Weight (kDa)

6.58

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 818
AarI CACCTGC 1 cut(s) 1943
Acc36I ACCTGC 1 cut(s) 1943
AccB1I GGYRCC 2 cut(s) 946, 2054
AccBSI CCGCTC 1 cut(s) 2060
AccI GTMKAC 2 cut(s) 288, 2662
AccII CGCG 2 cut(s) 2866, 2868
AciI CCGC 7 cut(s) 343, 2058, 2100, 2168, 2229, 2348, 2915
AclWI GGATC 5 cut(s) 134, 665, 678, 995, 2521
AcoI YGGCCR 1 cut(s) 775
AcuI CTGAAG 7 cut(s) 1100, 2448, 2543, 2591, 2733, 2757, 2880
AfaI GTAC 5 cut(s) 173, 294, 682, 1177, 2838
AfiI CCNNNNNNNGG 3 cut(s) 161, 319, 602
AflII CTTAAG 1 cut(s) 824
AflIII ACRYGT 1 cut(s) 1867
AjiI CACGTC 1 cut(s) 2456
AjnI CCWGG 5 cut(s) 11, 728, 2062, 2095, 2928
AjuI GAANNNNNNNTTGG 2 cut(s) 1539, 1571
AloI GAACNNNNNNTCC 4 cut(s) 1854, 1886, 2607, 2639
Alw21I GWGCWC 3 cut(s) 765, 1915, 2989
Alw26I GTCTC 3 cut(s) 922, 2389, 2442
Alw44I GTGCAC 1 cut(s) 2985
AlwI GGATC 5 cut(s) 134, 665, 678, 995, 2521
AlwNI CAGNNNCTG 1 cut(s) 1322
Ama87I CYCGRG 2 cut(s) 1472, 2125
AoxI GGCC 6 cut(s) 152, 775, 1885, 1929, 2250, 2931
ApaLI GTGCAC 1 cut(s) 2985
ApeKI GCWGC 8 cut(s) 422, 1949, 2111, 2240, 2324, 2351, 2513, 2516
ArsI GACNNNNNNTTYG 2 cut(s) 2607, 2639
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 2 cut(s) 2866, 2868
AspS9I GGNCC 7 cut(s) 695, 1253, 1929, 1937, 2077, 2443, 2841
AsuC2I CCSGG 3 cut(s) 1473, 1474, 2375
AsuHPI GGTGA 4 cut(s) 1208, 1290, 1415, 2174
AvaI CYCGRG 2 cut(s) 1472, 2125
AvaII GGWCC 6 cut(s) 695, 1253, 1937, 2077, 2443, 2841
AxyI CCTNAGG 1 cut(s) 981
BaeGI GKGCMC 1 cut(s) 2989
BalI TGGCCA 1 cut(s) 777
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 2 cut(s) 946, 2054
BanII GRGCYC 2 cut(s) 1634, 2380
BarI GAAGNNNNNNTAC 2 cut(s) 279, 311
Bbv12I GWGCWC 3 cut(s) 765, 1915, 2989
BbvI GCAGC 8 cut(s) 409, 1936, 2098, 2252, 2336, 2338, 2525, 2528
BccI CCATC 7 cut(s) 233, 1043, 1098, 1102, 1141, 1736, 2541
BceAI ACGGC 3 cut(s) 356, 1764, 2054
BciT130I CCWGG 5 cut(s) 13, 730, 2064, 2097, 2930
BclI TGATCA 2 cut(s) 706, 1768
BcnI CCSGG 3 cut(s) 1473, 1474, 2375
BcoDI GTCTC 3 cut(s) 922, 2389, 2442
BfaI CTAG 7 cut(s) 38, 678, 1088, 1610, 1832, 2120, 2186
BfmI CTRYAG 4 cut(s) 1516, 1950, 2262, 2319
BfrI CTTAAG 1 cut(s) 824
BfuAI ACCTGC 1 cut(s) 1943
Bme1390I CCNGG 8 cut(s) 13, 730, 1473, 1474, 2064, 2097, 2375, 2930
Bme18I GGWCC 6 cut(s) 695, 1253, 1937, 2077, 2443, 2841
BmeT110I CYCGRG 2 cut(s) 1472, 2125
BmgBI CACGTC 1 cut(s) 2456
BmgT120I GGNCC 7 cut(s) 695, 1253, 1929, 1937, 2077, 2443, 2841
BmiI GGNNCC 8 cut(s) 607, 672, 948, 1254, 1939, 2056, 2068, 2079
BmrFI CCNGG 8 cut(s) 13, 730, 1473, 1474, 2064, 2097, 2375, 2930
BmrI ACTGGG 3 cut(s) 1259, 1396, 2011
BmuI ACTGGG 3 cut(s) 1259, 1396, 2011
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 4 cut(s) 141, 244, 1192, 2046
BpuEI CTTGAG 3 cut(s) 436, 1795, 2927
BpuMI CCSGG 3 cut(s) 1473, 1474, 2375
BsaJI CCNNGG 4 cut(s) 313, 1472, 1888, 2844
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 319, 602
Bse118I RCCGGY 1 cut(s) 1496
Bse21I CCTNAGG 1 cut(s) 981
Bse3DI GCAATG 1 cut(s) 2034
BseBI CCWGG 5 cut(s) 13, 730, 2064, 2097, 2930
BseDI CCNNGG 4 cut(s) 313, 1472, 1888, 2844
BseLI CCNNNNNNNGG 3 cut(s) 161, 319, 602
BseMI GCAATG 1 cut(s) 2034
BsePI GCGCGC 1 cut(s) 2864
BseRI GAGGAG 1 cut(s) 1449
BseSI GKGCMC 1 cut(s) 2989
BseXI GCAGC 8 cut(s) 409, 1936, 2098, 2252, 2336, 2338, 2525, 2528
BseYI CCCAGC 1 cut(s) 2810
Bsh1236I CGCG 2 cut(s) 2866, 2868
Bsh1285I CGRYCG 1 cut(s) 2794
BshFI GGCC 6 cut(s) 154, 777, 1887, 1931, 2252, 2933
BshNI GGYRCC 2 cut(s) 946, 2054
BsiEI CGRYCG 1 cut(s) 2794
BsiHKAI GWGCWC 3 cut(s) 765, 1915, 2989
BsiHKCI CYCGRG 2 cut(s) 1472, 2125
BsiSI CCGG 3 cut(s) 1473, 1497, 2374
BslFI GGGAC 2 cut(s) 1923, 2628
BslI CCNNNNNNNGG 3 cut(s) 161, 319, 602
BsmAI GTCTC 3 cut(s) 922, 2389, 2442
BsmFI GGGAC 2 cut(s) 1923, 2628
BsnI GGCC 6 cut(s) 154, 777, 1887, 1931, 2252, 2933
BsoBI CYCGRG 2 cut(s) 1472, 2125
Bsp1286I GDGCHC 5 cut(s) 765, 1634, 1915, 2380, 2989
Bsp143I GATC 9 cut(s) 139, 181, 670, 706, 940, 1000, 1768, 2116, 2526
Bsp19I CCATGG 2 cut(s) 313, 2844
BspACI CCGC 7 cut(s) 343, 2058, 2100, 2168, 2229, 2348, 2915
BspANI GGCC 6 cut(s) 154, 777, 1887, 1931, 2252, 2933
BspFNI CGCG 2 cut(s) 2866, 2868
BspHI TCATGA 1 cut(s) 1846
BspLI GGNNCC 8 cut(s) 607, 672, 948, 1254, 1939, 2056, 2068, 2079
BspMAI CTGCAG 3 cut(s) 1520, 1954, 2266
BspMI ACCTGC 1 cut(s) 1943
BspPI GGATC 5 cut(s) 134, 665, 678, 995, 2521
BspT107I GGYRCC 2 cut(s) 946, 2054
BspTI CTTAAG 1 cut(s) 824
BsrBI CCGCTC 1 cut(s) 2060
BsrDI GCAATG 1 cut(s) 2034
BsrFI RCCGGY 1 cut(s) 1496
BssAI RCCGGY 1 cut(s) 1496
BssECI CCNNGG 4 cut(s) 313, 1472, 1888, 2844
BssHII GCGCGC 1 cut(s) 2864
BssMI GATC 9 cut(s) 139, 181, 670, 706, 940, 1000, 1768, 2116, 2526
BssNAI GTATAC 1 cut(s) 2663
BssT1I CCWWGG 3 cut(s) 313, 1888, 2844
Bst1107I GTATAC 1 cut(s) 2663
Bst2UI CCWGG 5 cut(s) 13, 730, 2064, 2097, 2930
Bst4CI ACNGT 9 cut(s) 74, 215, 250, 1544, 1718, 2032, 2400, 2585, 2841
Bst6I CTCTTC 4 cut(s) 402, 1032, 2014, 2462
BstAFI CTTAAG 1 cut(s) 824
BstAPI GCANNNNNTGC 3 cut(s) 769, 2237, 2270
BstC8I GCNNGC 6 cut(s) 280, 1467, 1516, 1759, 2866, 2985
BstDSI CCRYGG 2 cut(s) 313, 2844
BstFNI CGCG 2 cut(s) 2866, 2868
BstHHI GCGC 2 cut(s) 2866, 2868
BstKTI GATC 9 cut(s) 142, 184, 673, 709, 943, 1003, 1771, 2119, 2529
BstMAI GTCTC 3 cut(s) 922, 2389, 2442
BstMBI GATC 9 cut(s) 139, 181, 670, 706, 940, 1000, 1768, 2116, 2526
BstMCI CGRYCG 1 cut(s) 2794
BstNI CCWGG 5 cut(s) 13, 730, 2064, 2097, 2930
BstNSI RCATGY 5 cut(s) 1399, 1469, 1761, 1871, 2510
BstSCI CCNGG 8 cut(s) 11, 728, 1471, 1472, 2062, 2095, 2373, 2928
BstSFI CTRYAG 4 cut(s) 1516, 1950, 2262, 2319
BstSLI GKGCMC 1 cut(s) 2989
BstUI CGCG 2 cut(s) 2866, 2868
BstV1I GCAGC 8 cut(s) 409, 1936, 2098, 2252, 2336, 2338, 2525, 2528
BstX2I RGATCY 3 cut(s) 139, 670, 2526
BstYI RGATCY 3 cut(s) 139, 670, 2526
BstZ17I GTATAC 1 cut(s) 2663
Bsu36I CCTNAGG 1 cut(s) 981
BsuRI GGCC 6 cut(s) 154, 777, 1887, 1931, 2252, 2933
BtgI CCRYGG 2 cut(s) 313, 2844
BtgZI GCGATG 1 cut(s) 203
BtrI CACGTC 1 cut(s) 2456
BtsI GCAGTG 2 cut(s) 289, 1525
BtsIMutI CAGTG 6 cut(s) 289, 1077, 1247, 1525, 1723, 1999
BveI ACCTGC 1 cut(s) 1943
Cac8I GCNNGC 6 cut(s) 280, 1467, 1516, 1759, 2866, 2985
CaiI CAGNNNCTG 1 cut(s) 1322
CciI TCATGA 1 cut(s) 1846
CfoI GCGC 2 cut(s) 2866, 2868
Cfr10I RCCGGY 1 cut(s) 1496
Cfr13I GGNCC 7 cut(s) 695, 1253, 1929, 1937, 2077, 2443, 2841
Cfr9I CCCGGG 1 cut(s) 1472
Csp6I GTAC 5 cut(s) 172, 293, 681, 1176, 2837
CviQI GTAC 5 cut(s) 172, 293, 681, 1176, 2837
DpnI GATC 9 cut(s) 141, 183, 672, 708, 942, 1002, 1770, 2118, 2528
DpnII GATC 9 cut(s) 139, 181, 670, 706, 940, 1000, 1768, 2116, 2526
EaeI YGGCCR 1 cut(s) 775
Eam1104I CTCTTC 4 cut(s) 402, 1032, 2014, 2462
EarI CTCTTC 4 cut(s) 402, 1032, 2014, 2462
EciI GGCGGA 2 cut(s) 358, 2115
Eco130I CCWWGG 3 cut(s) 313, 1888, 2844
Eco147I AGGCCT 2 cut(s) 154, 2933
Eco24I GRGCYC 2 cut(s) 1634, 2380
Eco47I GGWCC 6 cut(s) 695, 1253, 1937, 2077, 2443, 2841
Eco57I CTGAAG 7 cut(s) 1100, 2448, 2543, 2591, 2733, 2757, 2880
Eco81I CCTNAGG 1 cut(s) 981
Eco88I CYCGRG 2 cut(s) 1472, 2125
EcoO109I RGGNCCY 4 cut(s) 1253, 1937, 2077, 2443
EcoRII CCWGG 5 cut(s) 11, 728, 2062, 2095, 2928
EcoT14I CCWWGG 3 cut(s) 313, 1888, 2844
EcoT22I ATGCAT 3 cut(s) 774, 1397, 2746
EcoT38I GRGCYC 2 cut(s) 1634, 2380
ErhI CCWWGG 3 cut(s) 313, 1888, 2844
FaqI GGGAC 2 cut(s) 1923, 2628
FauNDI CATATG 1 cut(s) 1227
FbaI TGATCA 2 cut(s) 706, 1768
FblI GTMKAC 2 cut(s) 288, 2662
FriOI GRGCYC 2 cut(s) 1634, 2380
FspBI CTAG 7 cut(s) 38, 678, 1088, 1610, 1832, 2120, 2186
GlaI GCGC 2 cut(s) 2865, 2867
GsaI CCCAGC 1 cut(s) 2814
GsuI CTGGAG 4 cut(s) 141, 244, 1192, 2046
HaeIII GGCC 6 cut(s) 154, 777, 1887, 1931, 2252, 2933
HapII CCGG 3 cut(s) 1473, 1497, 2374
HhaI GCGC 2 cut(s) 2866, 2868
Hin6I GCGC 2 cut(s) 2864, 2866
HinP1I GCGC 2 cut(s) 2864, 2866
HincII GTYRAC 4 cut(s) 523, 891, 2196, 2403
HindII GTYRAC 4 cut(s) 523, 891, 2196, 2403
HpaI GTTAAC 1 cut(s) 2403
HpaII CCGG 3 cut(s) 1473, 1497, 2374
HphI GGTGA 4 cut(s) 1208, 1290, 1415, 2174
Hpy99I CGWCG 2 cut(s) 2460, 2960
HpyCH4III ACNGT 9 cut(s) 74, 215, 250, 1544, 1718, 2032, 2400, 2585, 2841
HpyCH4IV ACGT 2 cut(s) 291, 2455
HpySE526I ACGT 2 cut(s) 291, 2455
HspAI GCGC 2 cut(s) 2864, 2866
Ksp22I TGATCA 2 cut(s) 706, 1768
KspAI GTTAAC 1 cut(s) 2403
Kzo9I GATC 9 cut(s) 139, 181, 670, 706, 940, 1000, 1768, 2116, 2526
LmnI GCTCC 5 cut(s) 955, 1474, 2065, 2066, 2957
Lsp1109I GCAGC 8 cut(s) 409, 1936, 2098, 2252, 2336, 2338, 2525, 2528
MaeI CTAG 7 cut(s) 38, 678, 1088, 1610, 1832, 2120, 2186
MaeII ACGT 2 cut(s) 291, 2455
MaeIII GTNAC 8 cut(s) 302, 871, 1245, 1492, 1732, 1919, 2180, 2579
MalI GATC 9 cut(s) 141, 183, 672, 708, 942, 1002, 1770, 2118, 2528
MbiI CCGCTC 1 cut(s) 2060
MboI GATC 9 cut(s) 139, 181, 670, 706, 940, 1000, 1768, 2116, 2526
MfeI CAATTG 1 cut(s) 1737
MflI RGATCY 3 cut(s) 139, 670, 2526
MhlI GDGCHC 5 cut(s) 765, 1634, 1915, 2380, 2989
MlsI TGGCCA 1 cut(s) 777
MluNI TGGCCA 1 cut(s) 777
MlyI GAGTC 6 cut(s) 668, 870, 1253, 1812, 2375, 2481
MmeI TCCRAC 7 cut(s) 625, 1050, 1215, 2554, 2666, 2774, 2979
Mox20I TGGCCA 1 cut(s) 777
Mph1103I ATGCAT 3 cut(s) 774, 1397, 2746
MroXI GAANNNNTTC 1 cut(s) 105
MscI TGGCCA 1 cut(s) 777
MseI TTAA 8 cut(s) 825, 1337, 1577, 2174, 2402, 2592, 2757, 3062
Msp20I TGGCCA 1 cut(s) 777
MspA1I CMGCKG 5 cut(s) 89, 161, 422, 2243, 2327
MspCI CTTAAG 1 cut(s) 824
MspI CCGG 3 cut(s) 1473, 1497, 2374
MspR9I CCNGG 8 cut(s) 13, 730, 1473, 1474, 2064, 2097, 2375, 2930
MunI CAATTG 1 cut(s) 1737
MvaI CCWGG 5 cut(s) 13, 730, 2064, 2097, 2930
MvnI CGCG 2 cut(s) 2866, 2868
NciI CCSGG 3 cut(s) 1473, 1474, 2375
NcoI CCATGG 2 cut(s) 313, 2844
NdeI CATATG 1 cut(s) 1227
NdeII GATC 9 cut(s) 139, 181, 670, 706, 940, 1000, 1768, 2116, 2526
NlaIV GGNNCC 8 cut(s) 607, 672, 948, 1254, 1939, 2056, 2068, 2079
NmuCI GTSAC 4 cut(s) 1245, 1492, 2180, 2579
NsiI ATGCAT 3 cut(s) 774, 1397, 2746
NspI RCATGY 5 cut(s) 1399, 1469, 1761, 1871, 2510
PaeI GCATGC 2 cut(s) 1469, 1761
PaeR7I CTCGAG 1 cut(s) 2125
PagI TCATGA 1 cut(s) 1846
PaqCI CACCTGC 1 cut(s) 1943
PauI GCGCGC 1 cut(s) 2864
PceI AGGCCT 2 cut(s) 154, 2933
PciI ACATGT 1 cut(s) 1867
PcsI WCGNNNNNNNCGW 2 cut(s) 202, 2386
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 8 cut(s) 557, 1139, 1157, 1560, 1795, 2276, 2575, 2938
PfoI TCCNGGA 1 cut(s) 2062
PleI GAGTC 6 cut(s) 667, 869, 1252, 1812, 2374, 2480
PpsI GAGTC 6 cut(s) 667, 869, 1252, 1812, 2374, 2480
PpuMI RGGWCCY 4 cut(s) 1253, 1937, 2077, 2443
PscI ACATGT 1 cut(s) 1867
PsiI TTATAA 1 cut(s) 818
Psp5II RGGWCCY 4 cut(s) 1253, 1937, 2077, 2443
Psp6I CCWGG 5 cut(s) 11, 728, 2062, 2095, 2928
PspFI CCCAGC 1 cut(s) 2810
PspGI CCWGG 5 cut(s) 11, 728, 2062, 2095, 2928
PspN4I GGNNCC 8 cut(s) 607, 672, 948, 1254, 1939, 2056, 2068, 2079
PspPI GGNCC 7 cut(s) 695, 1253, 1929, 1937, 2077, 2443, 2841
PspPPI RGGWCCY 4 cut(s) 1253, 1937, 2077, 2443
PspXI VCTCGAGB 1 cut(s) 2125
PstI CTGCAG 3 cut(s) 1520, 1954, 2266
PstNI CAGNNNCTG 1 cut(s) 1322
PsuI RGATCY 3 cut(s) 139, 670, 2526
PteI GCGCGC 1 cut(s) 2864
PvuII CAGCTG 5 cut(s) 89, 161, 422, 2243, 2327
RsaI GTAC 5 cut(s) 173, 294, 682, 1177, 2838
RsaNI GTAC 5 cut(s) 172, 293, 681, 1176, 2837
SaqAI TTAA 8 cut(s) 825, 1337, 1577, 2174, 2402, 2592, 2757, 3062
Sau3AI GATC 9 cut(s) 139, 181, 670, 706, 940, 1000, 1768, 2116, 2526
Sau96I GGNCC 7 cut(s) 695, 1253, 1929, 1937, 2077, 2443, 2841
SchI GAGTC 6 cut(s) 668, 870, 1253, 1812, 2375, 2481
ScrFI CCNGG 8 cut(s) 13, 730, 1473, 1474, 2064, 2097, 2375, 2930
SduI GDGCHC 5 cut(s) 765, 1634, 1915, 2380, 2989
SfcI CTRYAG 4 cut(s) 1516, 1950, 2262, 2319
Sfr274I CTCGAG 1 cut(s) 2125
SinI GGWCC 6 cut(s) 695, 1253, 1937, 2077, 2443, 2841
SlaI CTCGAG 1 cut(s) 2125
SmaI CCCGGG 1 cut(s) 1474
SmlI CTYRAG 5 cut(s) 451, 824, 1774, 2125, 2906
SmoI CTYRAG 5 cut(s) 451, 824, 1774, 2125, 2906
SphI GCATGC 2 cut(s) 1469, 1761
SseBI AGGCCT 2 cut(s) 154, 2933
SsiI CCGC 7 cut(s) 343, 2058, 2100, 2168, 2229, 2348, 2915
SspMI CTAG 7 cut(s) 38, 678, 1088, 1610, 1832, 2120, 2186
StuI AGGCCT 2 cut(s) 154, 2933
StyD4I CCNGG 8 cut(s) 11, 728, 1471, 1472, 2062, 2095, 2373, 2928
StyI CCWWGG 3 cut(s) 313, 1888, 2844
TaaI ACNGT 9 cut(s) 74, 215, 250, 1544, 1718, 2032, 2400, 2585, 2841
TaiI ACGT 2 cut(s) 294, 2458
TaqI TCGA 5 cut(s) 184, 196, 2047, 2126, 2936
TaqII GACCGA 1 cut(s) 2780
TatI WGTACW 2 cut(s) 680, 1175
TauI GCSGC 2 cut(s) 2060, 2351
TfiI GAWTC 8 cut(s) 557, 1139, 1157, 1560, 1795, 2276, 2575, 2938
Tru1I TTAA 8 cut(s) 825, 1337, 1577, 2174, 2402, 2592, 2757, 3062
Tru9I TTAA 8 cut(s) 825, 1337, 1577, 2174, 2402, 2592, 2757, 3062
TscAI CASTG 6 cut(s) 289, 1077, 1254, 1525, 1723, 2006
TseFI GTSAC 4 cut(s) 1245, 1492, 2180, 2579
TseI GCWGC 8 cut(s) 422, 1949, 2111, 2240, 2324, 2351, 2513, 2516
Tsp45I GTSAC 4 cut(s) 1245, 1492, 2180, 2579
TspGWI ACGGA 1 cut(s) 1998
TspMI CCCGGG 1 cut(s) 1472
TspRI CASTG 6 cut(s) 289, 1077, 1254, 1525, 1723, 2006
Vha464I CTTAAG 1 cut(s) 824
VneI GTGCAC 1 cut(s) 2985
VpaK11BI GGWCC 6 cut(s) 695, 1253, 1937, 2077, 2443, 2841
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 5 cut(s) 1399, 1469, 1761, 1871, 2510
XhoI CTCGAG 1 cut(s) 2125
XmaI CCCGGG 1 cut(s) 1472
XmiI GTMKAC 2 cut(s) 288, 2662
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 7 cut(s) 38, 678, 1088, 1610, 1832, 2120, 2186
Zsp2I ATGCAT 3 cut(s) 774, 1397, 2746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.