MD13G1144300.v1.1

calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001204
Physical Location & Seq
Forward (+)
11215719 .. 11223702
7984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1144300.v1.1.491

Sequence Viewer

Length: 3015 bp
ATGGCTGATACCAGGAGATACGCCCCCAATCAGCATCTAGACCTTGCCCAGATATTGCAAGAAGCAAAGAACCGTTGGCTTCGTCCAGCTGAAATTTGTGAAATACTTCGCAACTATCGGCATTTTGAATTGACCACAGATCCGCCCGTCAGGCCTCCAGCTGGTTCTTTGTACCTGTTTGATCGAAAAGCACTTCGATATTTTCGTAAAGATGGTCATCGCTGGAGGAAGAAAAAGGATGGAAAGACAGTAAAAGAAGCCCATGAAAAGTTGAAGGCTGGCAGTGTAGACGTACTTCATTGTTACTATGCCCATGGGGAGGACAATGCAAATTTTCAACGGCGGAGTTATTGGATGCTTGACACGCATTTACAGCACATTGTTCTTGTGCATTACAGAAATGTGGAAGAGGCAAACTCAGCTGCCCGTATGGTTCAAACATCCTTTGGTTCAAGTCCACTTCAAACATCCTTTGCTTCAAGTCCAGTTCATACATCCTTTGCTTCAAGTCCAAACAGAGTTGAATTGGGTGGACAAACATTATCGAAAGAATTTGAGGATGTGGATTCTCGGAGAGATGCAGGAACTTCATCTGTTGAGCAACCGATGTTTGGTTCCTTTTCTCATAATGCTTCTTTGCTTCCTCAACAAGTTGGAGGATTTCCTGAGTCATTCAGGGATCCCTCTAGTACATGGTATGGTGGACCTAAATTTGATCATGGTGCTGGATTATCTGCCTGGCAAGGAATGGATAGCTCAATGAGAAATGAGCACATCATGCATGGCCAAAACCTTTTTGTTGAAGAACCTAACAGAGCTGACTTTATAACTCTTAAGCTAACAGATGCTAAAGTGGATGCTCATAGTGGAGTCAAAGATGTAACTTGTGAAGATAGGTTGACCACTAACATAGATGTCCAAATTGTCTCAGCACCTTCTCAAAGAGAAGATCAGGTGCCAAAGGAGCATGACTTCAATGTGCTTCATCCTCAGGTTCAAGATTATTCTGATCCTCAGATTGTAGTAACTCCTTCCAACCAAGTTGAAGAGAATAGAGATTGTCGTGTGCGTAATGAACCAGTGGAACTGAAGAAACTAGACAGCTTTGGGAGATGGATGGATAAAGAAATTGGTGTGGATTGTGATGATTCCTTGATGGCTTCCGACTCTGGTAATTACTGGAGTACATTTGATGCTGAGAGTGGTGATAAGGAAGTGTCCAGTTTATCACACCATATGCAGTTGGATATAGAGTCACTGGGTCCTTCTCTTTCCCAAGAACAACTATTTACTATTTCTGATTTTTCACCAGATTGGGCTTATTCAGGAACTGAAACAAAGGTTTTAATAGTTGGCAGTTTTCTTGGAAGCAAGAAGGATTGTGCTGAGACTAACTGGGGGTGCATGTTTGGTGAAATAGAAGTTTCTGCTGAAATTCTATCTAACAATGCCATTAGATGTCAAACTCCTCTGCATGCTCCTGGGCGTGTTCCATTCTATGTGACCTGCAGAAATAGGTTAGCCTGCAGTGAAATTAGGGAGTTTGAATACCGTGAAAAACCCATTGGAATAGCCATCAACAGGTTAAGAGATGATGAATTACGCTTTCAGATACGACTAGCAAAATTGTTGAGCTTGGGCTCAGAGAGGAAGTGGTTGATATGTGCTGTTCCAGATTGTGATCAATGCAAGCTCAAAAGTTCCATATTTTCAATGAGAAGCAACAGTGAAAGTGATTGGGAAACAATTGATGGGGCTTCTGTGGCATGCGAAAGTGACCACTTGAGCCGTAGGGATGTCTTGATTCAGAATTTGTTGAAGGATAGACTCTGTGAATGGCTAGTATGTAAAATTCACGAAGGAGGCAAAGGAACACATGTTCTGGATAATGAAGGCCAAGGTGTTCTACATTTGACAGGTGCTCTTGGTTACGAGTGGGCCATAGGTCCCATAATTGCTGCAGGTGTCAGTCCCAATTTCAGAGATGCTCACGGAAGAACAGGGCTTCACTGGGCATCATATTTTGGAAGAGAGGAAACTGTCATTGCGTTACTTCGATTGGGTGCCGCTCCAGGAGCCGTCGAGGACCCAACATCTGTGTTTCCTGGCGGACAAACTGCTGCTGATCTAGCCTCGAGTAGAGGGCATAAAGGGATTGCTGGATATTTGGCAGAAGTGGATTTAACCAGTCACCTGGAGTTGTTGACCATGAATGAGAATAGAATGAACAATGTTACAGCAACTATTGCAGCTGAGAAGGCCAAACAGACAGCAGAAGTTTCTGCATCTGATGTTGCAGTAGATGAGCAACATTTTCTCAAAAACCCTATAGCAGCTGTTAGGAAATCAGCTCATGCGGCTGCTCTAATTCAGGAGGCTTTCAGGGCTCGTTCATTTCGTCAGAGACAGTTAACCAAGAGCAGAACAGATATTTCTGAAGTTCAGTCTCAGGACCTTGTTGCACGTCGTTCCTTGAAGAGAGTCCAGAAGTTCGCTCACTATGAGGATTATCTGCATGTCGCAGCAGCTCTGAAGATCCAACAAAACTATCGTGGATGGAAGGGAAGAAAGGATTATCTGAAGATTCGTGACCGTATTGTTAAAATTCAGGCTCATGTGAGGGGACATCAGGTTCGGAAGAATTACAAAAAGGTTTTGTGGTCTGTTGGTATACTTGAAAAGGTTATACTGCGTTGGAGGCGAAAGGGAACTGGTTTGCGAGGATTTCGGGTGGAAGGGCCTACTGAAGATGCATCTTCAGAGGTTAAGAAAAATGATGACTATGAATTTCTTAGTGTCGGTCGGAAGCAGAAATTTGCTGGGGTTGAGAAAGCTCTCGCAAGGGTAAAGTCCATGGCTCGTCAGCCTGAAGCGCGTGAACAATACATGAGGCTACTTTCAAACTTTGAGAAACTTGAGATGGCGGACGGGGAGAACCAGGCCTCGAATCAAAATGAAAGTTCCGACGAAAAAGTTATAGATGAAGTTCTGCGTGCACTCAATGAAGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 CG-1 IQ TIG

Protein Analysis

1005

Amino Acids

113.16

Weight (kDa)

6.59

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 21 - 134 6.6e-49 CG-1 domain
TIG PF01833 431 - 516 2.6e-06 IPT/TIG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 827
AarI CACCTGC 1 cut(s) 1952
Acc36I ACCTGC 2 cut(s) 1514, 1952
AccB1I GGYRCC 2 cut(s) 955, 2063
AccBSI CCGCTC 1 cut(s) 2069
AccI GTMKAC 2 cut(s) 288, 2671
AccII CGCG 1 cut(s) 2875
AciI CCGC 6 cut(s) 143, 343, 2067, 2109, 2357, 2924
AclWI GGATC 5 cut(s) 134, 674, 687, 1004, 2530
AcoI YGGCCR 1 cut(s) 784
AcuI CTGAAG 7 cut(s) 1109, 2457, 2552, 2600, 2742, 2766, 2889
AfaI GTAC 4 cut(s) 173, 294, 691, 1186
AfiI CCNNNNNNNGG 4 cut(s) 161, 319, 611, 1581
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 1 cut(s) 1876
AjiI CACGTC 1 cut(s) 2465
AjnI CCWGG 7 cut(s) 11, 737, 1480, 2071, 2104, 2193, 2937
AjuI GAANNNNNNNTTGG 2 cut(s) 1548, 1580
AloI GAACNNNNNNTCC 4 cut(s) 1863, 1895, 2616, 2648
Alw21I GWGCWC 3 cut(s) 774, 1924, 2998
Alw26I GTCTC 4 cut(s) 931, 1382, 2398, 2451
Alw44I GTGCAC 1 cut(s) 2994
AlwI GGATC 5 cut(s) 134, 674, 687, 1004, 2530
AlwNI CAGNNNCTG 1 cut(s) 1331
Ama87I CYCGRG 1 cut(s) 2134
AoxI GGCC 7 cut(s) 152, 784, 1894, 1938, 2259, 2738, 2940
ApaLI GTGCAC 1 cut(s) 2994
ApeKI GCWGC 8 cut(s) 422, 1958, 2120, 2249, 2333, 2360, 2522, 2525
ArsI GACNNNNNNTTYG 2 cut(s) 2616, 2648
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 1 cut(s) 2875
AspS9I GGNCC 7 cut(s) 704, 1262, 1938, 1946, 2086, 2452, 2738
AsuHPI GGTGA 4 cut(s) 1217, 1299, 1424, 2183
AvaI CYCGRG 1 cut(s) 2134
AvaII GGWCC 5 cut(s) 704, 1262, 1946, 2086, 2452
AxyI CCTNAGG 1 cut(s) 990
BaeGI GKGCMC 1 cut(s) 2998
BalI TGGCCA 1 cut(s) 786
BamHI GGATCC 1 cut(s) 679
BanI GGYRCC 2 cut(s) 955, 2063
BanII GRGCYC 2 cut(s) 1643, 2389
BarI GAAGNNNNNNTAC 2 cut(s) 279, 311
Bbv12I GWGCWC 3 cut(s) 774, 1924, 2998
BbvI GCAGC 8 cut(s) 409, 1945, 2107, 2261, 2345, 2347, 2534, 2537
BccI CCATC 9 cut(s) 206, 233, 1107, 1111, 1150, 1583, 1745, 2550, 2914
BceAI ACGGC 3 cut(s) 356, 1773, 2063
BciT130I CCWGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
BclI TGATCA 2 cut(s) 715, 1681
BcoDI GTCTC 4 cut(s) 931, 1382, 2398, 2451
BfaI CTAG 6 cut(s) 38, 687, 1097, 1619, 1841, 2129
BfmI CTRYAG 4 cut(s) 1507, 1525, 1959, 2328
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 2 cut(s) 1514, 1952
BglI GCCNNNNNGGC 1 cut(s) 151
Bme1390I CCNGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
Bme18I GGWCC 5 cut(s) 704, 1262, 1946, 2086, 2452
BmeT110I CYCGRG 1 cut(s) 2134
BmgBI CACGTC 1 cut(s) 2465
BmgT120I GGNCC 7 cut(s) 704, 1262, 1938, 1946, 2086, 2452, 2738
BmiI GGNNCC 8 cut(s) 616, 681, 957, 1263, 1948, 2065, 2077, 2088
BmrFI CCNGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
BmrI ACTGGG 3 cut(s) 1268, 1405, 2020
BmuI ACTGGG 3 cut(s) 1268, 1405, 2020
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 5 cut(s) 141, 244, 1201, 2055, 2216
BpuEI CTTGAG 2 cut(s) 1804, 2936
BsaBI GATNNNNATC 2 cut(s) 216, 1680
BsaJI CCNNGG 4 cut(s) 313, 1481, 1897, 2853
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 319, 611, 1581
Bse1I ACTGG 9 cut(s) 485, 1079, 1184, 1221, 1263, 1400, 2015, 2187, 2716
Bse21I CCTNAGG 1 cut(s) 990
Bse3DI GCAATG 1 cut(s) 2043
Bse8I GATNNNNATC 2 cut(s) 216, 1680
BseBI CCWGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
BseDI CCNNGG 4 cut(s) 313, 1481, 1897, 2853
BseJI GATNNNNATC 2 cut(s) 216, 1680
BseLI CCNNNNNNNGG 4 cut(s) 161, 319, 611, 1581
BseMI GCAATG 1 cut(s) 2043
BseNI ACTGG 9 cut(s) 485, 1079, 1184, 1221, 1263, 1400, 2015, 2187, 2716
BseRI GAGGAG 1 cut(s) 1458
BseSI GKGCMC 1 cut(s) 2998
BseXI GCAGC 8 cut(s) 409, 1945, 2107, 2261, 2345, 2347, 2534, 2537
BseYI CCCAGC 1 cut(s) 2819
Bsh1236I CGCG 1 cut(s) 2875
Bsh1285I CGRYCG 1 cut(s) 2803
BshFI GGCC 7 cut(s) 154, 786, 1896, 1940, 2261, 2740, 2942
BshNI GGYRCC 2 cut(s) 955, 2063
BsiEI CGRYCG 1 cut(s) 2803
BsiHKAI GWGCWC 3 cut(s) 774, 1924, 2998
BsiHKCI CYCGRG 1 cut(s) 2134
BslFI GGGAC 3 cut(s) 1932, 1956, 2637
BslI CCNNNNNNNGG 4 cut(s) 161, 319, 611, 1581
BsmAI GTCTC 4 cut(s) 931, 1382, 2398, 2451
BsmFI GGGAC 3 cut(s) 1932, 1956, 2637
BsnI GGCC 7 cut(s) 154, 786, 1896, 1940, 2261, 2740, 2942
BsoBI CYCGRG 1 cut(s) 2134
Bsp1286I GDGCHC 5 cut(s) 774, 1643, 1924, 2389, 2998
Bsp143I GATC 9 cut(s) 139, 181, 679, 715, 949, 1009, 1681, 2125, 2535
Bsp19I CCATGG 2 cut(s) 313, 2853
BspACI CCGC 6 cut(s) 143, 343, 2067, 2109, 2357, 2924
BspANI GGCC 7 cut(s) 154, 786, 1896, 1940, 2261, 2740, 2942
BspFNI CGCG 1 cut(s) 2875
BspLI GGNNCC 8 cut(s) 616, 681, 957, 1263, 1948, 2065, 2077, 2088
BspMAI CTGCAG 3 cut(s) 1511, 1529, 1963
BspMI ACCTGC 2 cut(s) 1514, 1952
BspPI GGATC 5 cut(s) 134, 674, 687, 1004, 2530
BspT107I GGYRCC 2 cut(s) 955, 2063
BspTI CTTAAG 1 cut(s) 833
BsrBI CCGCTC 1 cut(s) 2069
BsrDI GCAATG 1 cut(s) 2043
BsrI ACTGG 9 cut(s) 485, 1079, 1184, 1221, 1263, 1400, 2015, 2187, 2716
BssECI CCNNGG 4 cut(s) 313, 1481, 1897, 2853
BssMI GATC 9 cut(s) 139, 181, 679, 715, 949, 1009, 1681, 2125, 2535
BssNAI GTATAC 1 cut(s) 2672
BssT1I CCWWGG 3 cut(s) 313, 1897, 2853
Bst1107I GTATAC 1 cut(s) 2672
Bst2UI CCWGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
Bst4CI ACNGT 7 cut(s) 74, 250, 1553, 1727, 2041, 2409, 2594
Bst6I CTCTTC 4 cut(s) 402, 1041, 2023, 2471
BstAFI CTTAAG 1 cut(s) 833
BstAPI GCANNNNNTGC 2 cut(s) 778, 2246
BstC8I GCNNGC 6 cut(s) 280, 1476, 1525, 1691, 1768, 2994
BstDSI CCRYGG 2 cut(s) 313, 2853
BstFNI CGCG 1 cut(s) 2875
BstHHI GCGC 1 cut(s) 2875
BstKTI GATC 9 cut(s) 142, 184, 682, 718, 952, 1012, 1684, 2128, 2538
BstMAI GTCTC 4 cut(s) 931, 1382, 2398, 2451
BstMBI GATC 9 cut(s) 139, 181, 679, 715, 949, 1009, 1681, 2125, 2535
BstMCI CGRYCG 1 cut(s) 2803
BstNI CCWGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
BstNSI RCATGY 5 cut(s) 1408, 1478, 1770, 1880, 2519
BstSCI CCNGG 7 cut(s) 11, 737, 1480, 2071, 2104, 2193, 2937
BstSFI CTRYAG 4 cut(s) 1507, 1525, 1959, 2328
BstSLI GKGCMC 1 cut(s) 2998
BstUI CGCG 1 cut(s) 2875
BstV1I GCAGC 8 cut(s) 409, 1945, 2107, 2261, 2345, 2347, 2534, 2537
BstX2I RGATCY 3 cut(s) 139, 679, 2535
BstXI CCANNNNNNTGG 1 cut(s) 2194
BstYI RGATCY 3 cut(s) 139, 679, 2535
BstZ17I GTATAC 1 cut(s) 2672
Bsu36I CCTNAGG 1 cut(s) 990
BsuRI GGCC 7 cut(s) 154, 786, 1896, 1940, 2261, 2740, 2942
BtgI CCRYGG 2 cut(s) 313, 2853
BtgZI GCGATG 1 cut(s) 203
BtrI CACGTC 1 cut(s) 2465
BtsI GCAGTG 2 cut(s) 289, 1534
BtsIMutI CAGTG 6 cut(s) 289, 1086, 1256, 1534, 1732, 2008
BveI ACCTGC 2 cut(s) 1514, 1952
Cac8I GCNNGC 6 cut(s) 280, 1476, 1525, 1691, 1768, 2994
CaiI CAGNNNCTG 1 cut(s) 1331
CfoI GCGC 1 cut(s) 2875
Cfr13I GGNCC 7 cut(s) 704, 1262, 1938, 1946, 2086, 2452, 2738
Csp6I GTAC 4 cut(s) 172, 293, 690, 1185
CviQI GTAC 4 cut(s) 172, 293, 690, 1185
DpnI GATC 9 cut(s) 141, 183, 681, 717, 951, 1011, 1683, 2127, 2537
DpnII GATC 9 cut(s) 139, 181, 679, 715, 949, 1009, 1681, 2125, 2535
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 4 cut(s) 402, 1041, 2023, 2471
EarI CTCTTC 4 cut(s) 402, 1041, 2023, 2471
EciI GGCGGA 4 cut(s) 132, 358, 2124, 2939
Eco130I CCWWGG 3 cut(s) 313, 1897, 2853
Eco147I AGGCCT 2 cut(s) 154, 2942
Eco24I GRGCYC 2 cut(s) 1643, 2389
Eco47I GGWCC 5 cut(s) 704, 1262, 1946, 2086, 2452
Eco57I CTGAAG 7 cut(s) 1109, 2457, 2552, 2600, 2742, 2766, 2889
Eco81I CCTNAGG 1 cut(s) 990
Eco88I CYCGRG 1 cut(s) 2134
EcoO109I RGGNCCY 5 cut(s) 1262, 1946, 2086, 2452, 2738
EcoRII CCWGG 7 cut(s) 11, 737, 1480, 2071, 2104, 2193, 2937
EcoT14I CCWWGG 3 cut(s) 313, 1897, 2853
EcoT22I ATGCAT 2 cut(s) 783, 2755
EcoT38I GRGCYC 2 cut(s) 1643, 2389
ErhI CCWWGG 3 cut(s) 313, 1897, 2853
FaqI GGGAC 3 cut(s) 1932, 1956, 2637
FauNDI CATATG 1 cut(s) 1236
FbaI TGATCA 2 cut(s) 715, 1681
FblI GTMKAC 2 cut(s) 288, 2671
FriOI GRGCYC 2 cut(s) 1643, 2389
FspBI CTAG 6 cut(s) 38, 687, 1097, 1619, 1841, 2129
GlaI GCGC 1 cut(s) 2874
GsaI CCCAGC 1 cut(s) 2823
GsuI CTGGAG 5 cut(s) 141, 244, 1201, 2055, 2216
HaeIII GGCC 7 cut(s) 154, 786, 1896, 1940, 2261, 2740, 2942
HhaI GCGC 1 cut(s) 2875
Hin6I GCGC 1 cut(s) 2873
HinP1I GCGC 1 cut(s) 2873
HincII GTYRAC 3 cut(s) 900, 2205, 2412
HindII GTYRAC 3 cut(s) 900, 2205, 2412
HpaI GTTAAC 1 cut(s) 2412
HphI GGTGA 4 cut(s) 1217, 1299, 1424, 2183
Hpy99I CGWCG 3 cut(s) 2084, 2469, 2969
HpyCH4III ACNGT 7 cut(s) 74, 250, 1553, 1727, 2041, 2409, 2594
HpyCH4IV ACGT 2 cut(s) 291, 2464
HpySE526I ACGT 2 cut(s) 291, 2464
HspAI GCGC 1 cut(s) 2873
Ksp22I TGATCA 2 cut(s) 715, 1681
KspAI GTTAAC 1 cut(s) 2412
Kzo9I GATC 9 cut(s) 139, 181, 679, 715, 949, 1009, 1681, 2125, 2535
LmnI GCTCC 4 cut(s) 964, 1483, 2074, 2075
Lsp1109I GCAGC 8 cut(s) 409, 1945, 2107, 2261, 2345, 2347, 2534, 2537
MaeI CTAG 6 cut(s) 38, 687, 1097, 1619, 1841, 2129
MaeII ACGT 2 cut(s) 291, 2464
MalI GATC 9 cut(s) 141, 183, 681, 717, 951, 1011, 1683, 2127, 2537
MbiI CCGCTC 1 cut(s) 2069
MboI GATC 9 cut(s) 139, 181, 679, 715, 949, 1009, 1681, 2125, 2535
MfeI CAATTG 1 cut(s) 1746
MflI RGATCY 3 cut(s) 139, 679, 2535
MhlI GDGCHC 5 cut(s) 774, 1643, 1924, 2389, 2998
MlsI TGGCCA 1 cut(s) 786
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 6 cut(s) 677, 879, 1160, 1262, 1821, 2490
MmeI TCCRAC 8 cut(s) 634, 1059, 1188, 1224, 2563, 2675, 2783, 2988
Mox20I TGGCCA 1 cut(s) 786
Mph1103I ATGCAT 2 cut(s) 783, 2755
MroXI GAANNNNTTC 1 cut(s) 105
MscI TGGCCA 1 cut(s) 786
MseI TTAA 7 cut(s) 834, 1346, 1586, 2183, 2411, 2601, 2766
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 5 cut(s) 89, 161, 422, 2252, 2336
MspCI CTTAAG 1 cut(s) 833
MspR9I CCNGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
MunI CAATTG 1 cut(s) 1746
MvaI CCWGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
MvnI CGCG 1 cut(s) 2875
NcoI CCATGG 2 cut(s) 313, 2853
NdeI CATATG 1 cut(s) 1236
NdeII GATC 9 cut(s) 139, 181, 679, 715, 949, 1009, 1681, 2125, 2535
NlaIV GGNNCC 8 cut(s) 616, 681, 957, 1263, 1948, 2065, 2077, 2088
NmuCI GTSAC 5 cut(s) 1254, 1501, 1775, 2189, 2588
NsiI ATGCAT 2 cut(s) 783, 2755
NspI RCATGY 5 cut(s) 1408, 1478, 1770, 1880, 2519
PaeI GCATGC 2 cut(s) 1478, 1770
PaeR7I CTCGAG 1 cut(s) 2134
PaqCI CACCTGC 1 cut(s) 1952
PceI AGGCCT 2 cut(s) 154, 2942
PciI ACATGT 1 cut(s) 1876
PcsI WCGNNNNNNNCGW 2 cut(s) 202, 2395
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 5 cut(s) 566, 1148, 1804, 2584, 2947
PfoI TCCNGGA 1 cut(s) 2071
PleI GAGTC 6 cut(s) 676, 878, 1160, 1261, 1821, 2489
PpsI GAGTC 6 cut(s) 676, 878, 1160, 1261, 1821, 2489
PpuMI RGGWCCY 4 cut(s) 1262, 1946, 2086, 2452
PscI ACATGT 1 cut(s) 1876
PsiI TTATAA 1 cut(s) 827
Psp5II RGGWCCY 4 cut(s) 1262, 1946, 2086, 2452
Psp6I CCWGG 7 cut(s) 11, 737, 1480, 2071, 2104, 2193, 2937
PspFI CCCAGC 1 cut(s) 2819
PspGI CCWGG 7 cut(s) 11, 737, 1480, 2071, 2104, 2193, 2937
PspN4I GGNNCC 8 cut(s) 616, 681, 957, 1263, 1948, 2065, 2077, 2088
PspPI GGNCC 7 cut(s) 704, 1262, 1938, 1946, 2086, 2452, 2738
PspPPI RGGWCCY 4 cut(s) 1262, 1946, 2086, 2452
PspXI VCTCGAGB 1 cut(s) 2134
PstI CTGCAG 3 cut(s) 1511, 1529, 1963
PstNI CAGNNNCTG 1 cut(s) 1331
PsuI RGATCY 3 cut(s) 139, 679, 2535
PvuII CAGCTG 5 cut(s) 89, 161, 422, 2252, 2336
RsaI GTAC 4 cut(s) 173, 294, 691, 1186
RsaNI GTAC 4 cut(s) 172, 293, 690, 1185
SaqAI TTAA 7 cut(s) 834, 1346, 1586, 2183, 2411, 2601, 2766
Sau3AI GATC 9 cut(s) 139, 181, 679, 715, 949, 1009, 1681, 2125, 2535
Sau96I GGNCC 7 cut(s) 704, 1262, 1938, 1946, 2086, 2452, 2738
SchI GAGTC 6 cut(s) 677, 879, 1160, 1262, 1821, 2490
ScrFI CCNGG 7 cut(s) 13, 739, 1482, 2073, 2106, 2195, 2939
SduI GDGCHC 5 cut(s) 774, 1643, 1924, 2389, 2998
SfcI CTRYAG 4 cut(s) 1507, 1525, 1959, 2328
Sfr274I CTCGAG 1 cut(s) 2134
SinI GGWCC 5 cut(s) 704, 1262, 1946, 2086, 2452
SlaI CTCGAG 1 cut(s) 2134
SmlI CTYRAG 4 cut(s) 833, 1783, 2134, 2915
SmoI CTYRAG 4 cut(s) 833, 1783, 2134, 2915
SphI GCATGC 2 cut(s) 1478, 1770
SseBI AGGCCT 2 cut(s) 154, 2942
SsiI CCGC 6 cut(s) 143, 343, 2067, 2109, 2357, 2924
SspMI CTAG 6 cut(s) 38, 687, 1097, 1619, 1841, 2129
StuI AGGCCT 2 cut(s) 154, 2942
StyD4I CCNGG 7 cut(s) 11, 737, 1480, 2071, 2104, 2193, 2937
StyI CCWWGG 3 cut(s) 313, 1897, 2853
TaaI ACNGT 7 cut(s) 74, 250, 1553, 1727, 2041, 2409, 2594
TaiI ACGT 2 cut(s) 294, 2467
TaqI TCGA 7 cut(s) 184, 196, 545, 2056, 2082, 2135, 2945
TaqII GACCGA 1 cut(s) 2789
TatI WGTACW 2 cut(s) 689, 1184
TauI GCSGC 2 cut(s) 2069, 2360
TfiI GAWTC 5 cut(s) 566, 1148, 1804, 2584, 2947
Tru1I TTAA 7 cut(s) 834, 1346, 1586, 2183, 2411, 2601, 2766
Tru9I TTAA 7 cut(s) 834, 1346, 1586, 2183, 2411, 2601, 2766
TscAI CASTG 6 cut(s) 289, 1086, 1263, 1534, 1732, 2015
TseFI GTSAC 5 cut(s) 1254, 1501, 1775, 2189, 2588
TseI GCWGC 8 cut(s) 422, 1958, 2120, 2249, 2333, 2360, 2522, 2525
Tsp45I GTSAC 5 cut(s) 1254, 1501, 1775, 2189, 2588
TspGWI ACGGA 1 cut(s) 2007
TspRI CASTG 6 cut(s) 289, 1086, 1263, 1534, 1732, 2015
Vha464I CTTAAG 1 cut(s) 833
VneI GTGCAC 1 cut(s) 2994
VpaK11BI GGWCC 5 cut(s) 704, 1262, 1946, 2086, 2452
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 5 cut(s) 1408, 1478, 1770, 1880, 2519
XhoI CTCGAG 1 cut(s) 2134
XmiI GTMKAC 2 cut(s) 288, 2671
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 6 cut(s) 38, 687, 1097, 1619, 1841, 2129
Zsp2I ATGCAT 2 cut(s) 783, 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.