pycom16g12350

calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001204
Physical Location & Seq
Forward (+)
8670939 .. 8673236
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g12350.1

Sequence Viewer

Length: 945 bp
ATGCTTCACTGCTTCCACAAAAAAATTGGAGGATTTCCTGAGTCATTCAGGGATCCCTCTAGTACGTGGTATGGTGGACCTAAATGTGATCATGGTGCTGGATTATCCTCCTGGCACGGAGTGGATAGCTCAACGAGAAATGAGCACAGCATGCATGCCCAAAACCTTTTTGTTGAAGAACCTAACAGAGTTGACTTCATAACTAATAAGCTAACAGATTCTAGAGTGGATGTTCGTAGTGGAGTCAACGATGTAACTTGCGAAGATAGGTTGACCACTAACATAGATGTCCAAATTTTCTCAGCACCTTCTCAAAGAGAAGATCAGGTGCCAGAGGAGCATGTCTTCGATGTGTTTCATCCTCAGGTTCAAGATTATACTGATCCTCAGACTGTAGTAACTCCTTCCAACCAAGTCGGAGAGAATTATAGAGATGGTGGTGTGCATAATATGGAACTGAAGAAACTAGACAGCTTTGGGAGATGGATGGATAAAGAAATTGGTGTGGATTGTGATGATTCCTTGATGGCTTCTGACTCTGGTAATTACTGGAGTACATTTGATGCTGAGAGTGGTGATAAGGAAGTATCCAGTTTATCACACCATATGCAGTTGGATATAGATTCACTGGGCCCCCTTCTTTCCCAAGAACAACTATTTAGTATTTCTGATTTTTCGCCAGATTGGGCTTATTCAGGAACTGAAACAAAGGTTTTAATAGTTGGTAGTTTTCTGGGAAGCAAGAAGGATTCTGGTGACACTAATTGGGGATGCATGTTTGGTGAAATAGAAGTTTCTGCTGAAATTCTATCTAACAATGCCATTAGATGTCAAACTCCTTTGCATGCTCCTGGGCGTGTTCCATTCTATGTGACCGGCAGAAATAGGTTAGCCTGCAGTGATCGATCAGGGAGTTTGAATACCGCGAAAAACCCATTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 CG-1 IQ TIG

Protein Analysis

315

Amino Acids

34.91

Weight (kDa)

4.8

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIG PF01833 221 - 295 8.5e-08 IPT/TIG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AccII CGCG 1 cut(s) 926
AciI CCGC 1 cut(s) 924
AclWI GGATC 3 cut(s) 47, 60, 377
AcsI RAATTY 2 cut(s) 294, 804
AcuI CTGAAG 1 cut(s) 479
AdeI CACNNNGTG 1 cut(s) 121
AfaI GTAC 2 cut(s) 64, 556
AgsI TTSAA 3 cut(s) 176, 371, 919
AjnI CCWGG 2 cut(s) 110, 850
AjuI GAANNNNNNNTTGG 1 cut(s) 920
AluBI AGCT 3 cut(s) 129, 211, 474
AluI AGCT 3 cut(s) 129, 211, 474
Alw21I GWGCWC 1 cut(s) 147
AlwI GGATC 3 cut(s) 47, 60, 377
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 1 cut(s) 631
ApaI GGGCCC 1 cut(s) 635
ApoI RAATTY 2 cut(s) 294, 804
AspS9I GGNCC 3 cut(s) 77, 631, 632
AsuHPI GGTGA 3 cut(s) 587, 767, 794
AvaII GGWCC 1 cut(s) 77
AxyI CCTNAGG 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 635
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 147
BccI CCATC 4 cut(s) 428, 477, 481, 520
BciT130I CCWGG 2 cut(s) 112, 852
BciVI GTATCC 1 cut(s) 598
BclI TGATCA 1 cut(s) 88
BfaI CTAG 3 cut(s) 60, 222, 467
BfmI CTRYAG 2 cut(s) 393, 895
BfuI GTATCC 1 cut(s) 598
Bme1390I CCNGG 2 cut(s) 112, 852
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 3 cut(s) 77, 631, 632
BmiI GGNNCC 4 cut(s) 54, 330, 633, 634
BmrFI CCNGG 2 cut(s) 112, 852
BmrI ACTGGG 1 cut(s) 638
BmsI GCATC 2 cut(s) 553, 761
BmuI ACTGGG 1 cut(s) 638
BpiI GAAGAC 1 cut(s) 337
BpmI CTGGAG 1 cut(s) 571
Bsa29I ATCGAT 1 cut(s) 904
BsaAI YACGTR 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 851
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 3 cut(s) 554, 591, 633
Bse21I CCTNAGG 1 cut(s) 363
BseBI CCWGG 2 cut(s) 112, 852
BseCI ATCGAT 1 cut(s) 904
BseDI CCNNGG 1 cut(s) 851
BseGI GGATG 4 cut(s) 235, 358, 492, 776
BseMII CTCAG 5 cut(s) 30, 315, 377, 401, 558
BseNI ACTGG 3 cut(s) 554, 591, 633
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 635
Bsh1236I CGCG 1 cut(s) 926
BshFI GGCC 1 cut(s) 633
BshNI GGYRCC 1 cut(s) 328
BshVI ATCGAT 1 cut(s) 904
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 1 cut(s) 876
BsnI GGCC 1 cut(s) 633
Bsp120I GGGCCC 1 cut(s) 631
Bsp1286I GDGCHC 2 cut(s) 147, 635
Bsp143I GATC 6 cut(s) 52, 88, 322, 382, 901, 905
BspACI CCGC 1 cut(s) 924
BspANI GGCC 1 cut(s) 633
BspCNI CTCAG 5 cut(s) 31, 314, 376, 400, 559
BspDI ATCGAT 1 cut(s) 904
BspFNI CGCG 1 cut(s) 926
BspLI GGNNCC 4 cut(s) 54, 330, 633, 634
BspMAI CTGCAG 1 cut(s) 899
BspPI GGATC 3 cut(s) 47, 60, 377
BspT107I GGYRCC 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 875
BsrI ACTGG 3 cut(s) 554, 591, 633
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 1 cut(s) 851
BssMI GATC 6 cut(s) 52, 88, 322, 382, 901, 905
Bst2UI CCWGG 2 cut(s) 112, 852
Bst4CI ACNGT 1 cut(s) 394
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBAI YACGTR 1 cut(s) 66
BstC8I GCNNGC 4 cut(s) 152, 156, 846, 895
BstDEI CTNAG 5 cut(s) 39, 301, 363, 387, 567
BstF5I GGATG 4 cut(s) 235, 358, 492, 776
BstFNI CGCG 1 cut(s) 926
BstKTI GATC 6 cut(s) 55, 91, 325, 385, 904, 908
BstMBI GATC 6 cut(s) 52, 88, 322, 382, 901, 905
BstMWI GCNNNNNNNGC 2 cut(s) 151, 337
BstNI CCWGG 2 cut(s) 112, 852
BstNSI RCATGY 5 cut(s) 154, 158, 344, 778, 848
BstSCI CCNGG 2 cut(s) 110, 850
BstSFI CTRYAG 2 cut(s) 393, 895
BstSLI GKGCMC 1 cut(s) 635
BstUI CGCG 1 cut(s) 926
BstV2I GAAGAC 1 cut(s) 337
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
Bsu15I ATCGAT 1 cut(s) 904
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 598
BsuRI GGCC 1 cut(s) 633
BsuTUI ATCGAT 1 cut(s) 904
BtsCI GGATG 4 cut(s) 235, 358, 492, 776
BtsI GCAGTG 2 cut(s) 7, 904
BtsIMutI CAGTG 3 cut(s) 7, 626, 904
Cac8I GCNNGC 4 cut(s) 152, 156, 846, 895
CaiI CAGNNNCTG 1 cut(s) 701
Cfr10I RCCGGY 1 cut(s) 875
Cfr13I GGNCC 3 cut(s) 77, 631, 632
ClaI ATCGAT 1 cut(s) 904
Csp6I GTAC 2 cut(s) 63, 555
CviAII CATG 6 cut(s) 92, 151, 155, 341, 775, 845
CviJI RGCY 7 cut(s) 129, 211, 474, 530, 633, 689, 893
CviKI_1 RGCY 7 cut(s) 129, 211, 474, 530, 633, 689, 893
CviQI GTAC 2 cut(s) 63, 555
DdeI CTNAG 5 cut(s) 39, 301, 363, 387, 567
DpnI GATC 6 cut(s) 54, 90, 324, 384, 903, 907
DpnII GATC 6 cut(s) 52, 88, 322, 382, 901, 905
DraIII CACNNNGTG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 635
Eco47I GGWCC 1 cut(s) 77
Eco57I CTGAAG 1 cut(s) 479
Eco81I CCTNAGG 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 632
EcoRII CCWGG 2 cut(s) 110, 850
EcoT22I ATGCAT 2 cut(s) 156, 776
EcoT38I GRGCYC 1 cut(s) 635
FaeI CATG 6 cut(s) 95, 154, 158, 344, 778, 848
FatI CATG 6 cut(s) 91, 150, 154, 340, 774, 844
FauNDI CATATG 1 cut(s) 606
FbaI TGATCA 1 cut(s) 88
FokI GGATG 4 cut(s) 242, 345, 499, 783
FriOI GRGCYC 1 cut(s) 635
FspBI CTAG 3 cut(s) 60, 222, 467
GsuI CTGGAG 1 cut(s) 571
HaeIII GGCC 1 cut(s) 633
HapII CCGG 1 cut(s) 876
Hin1II CATG 6 cut(s) 95, 154, 158, 344, 778, 848
HincII GTYRAC 3 cut(s) 193, 247, 273
HindII GTYRAC 3 cut(s) 193, 247, 273
HinfI GANTC 7 cut(s) 41, 218, 243, 518, 536, 623, 749
HpaII CCGG 1 cut(s) 876
HphI GGTGA 3 cut(s) 587, 767, 794
Hpy166II GTNNAC 4 cut(s) 77, 193, 247, 273
Hpy188I TCNGA 4 cut(s) 390, 419, 535, 670
Hpy188III TCNNGA 4 cut(s) 38, 222, 371, 696
Hpy8I GTNNAC 4 cut(s) 77, 193, 247, 273
HpyAV CCTTC 4 cut(s) 318, 414, 647, 739
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 6 cut(s) 154, 445, 610, 774, 844, 897
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 337
HpyF3I CTNAG 5 cut(s) 39, 301, 363, 387, 567
HpySE526I ACGT 1 cut(s) 65
Hsp92II CATG 6 cut(s) 95, 154, 158, 344, 778, 848
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 6 cut(s) 52, 88, 322, 382, 901, 905
LmnI GCTCC 2 cut(s) 337, 853
LweI GCATC 2 cut(s) 553, 761
MaeI CTAG 3 cut(s) 60, 222, 467
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 4 cut(s) 253, 397, 755, 871
MalI GATC 6 cut(s) 54, 90, 324, 384, 903, 907
MboI GATC 6 cut(s) 52, 88, 322, 382, 901, 905
MboII GAAGA 5 cut(s) 188, 275, 332, 337, 472
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 2 cut(s) 147, 635
MluCI AATT 7 cut(s) 24, 294, 424, 498, 544, 763, 804
MlyI GAGTC 3 cut(s) 50, 252, 530
MmeI TCCRAC 3 cut(s) 397, 432, 594
MnlI CCTC 6 cut(s) 23, 67, 118, 328, 372, 396
Mph1103I ATGCAT 2 cut(s) 156, 776
MseI TTAA 1 cut(s) 716
MspI CCGG 1 cut(s) 876
MspR9I CCNGG 2 cut(s) 112, 852
MvaI CCWGG 2 cut(s) 112, 852
MvnI CGCG 1 cut(s) 926
MwoI GCNNNNNNNGC 2 cut(s) 151, 337
NdeI CATATG 1 cut(s) 606
NdeII GATC 6 cut(s) 52, 88, 322, 382, 901, 905
NlaIII CATG 6 cut(s) 95, 154, 158, 344, 778, 848
NlaIV GGNNCC 4 cut(s) 54, 330, 633, 634
NmuCI GTSAC 2 cut(s) 755, 871
NsiI ATGCAT 2 cut(s) 156, 776
NspI RCATGY 5 cut(s) 154, 158, 344, 778, 848
PaeI GCATGC 3 cut(s) 154, 158, 848
PfeI GAWTC 4 cut(s) 218, 518, 623, 749
PleI GAGTC 3 cut(s) 49, 251, 530
PpsI GAGTC 3 cut(s) 49, 251, 530
Ppu21I YACGTR 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 110, 850
PspGI CCWGG 2 cut(s) 110, 850
PspN4I GGNNCC 4 cut(s) 54, 330, 633, 634
PspOMI GGGCCC 1 cut(s) 631
PspPI GGNCC 3 cut(s) 77, 631, 632
PstI CTGCAG 1 cut(s) 899
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 2 cut(s) 64, 556
RsaNI GTAC 2 cut(s) 63, 555
SaqAI TTAA 1 cut(s) 716
Sau3AI GATC 6 cut(s) 52, 88, 322, 382, 901, 905
Sau96I GGNCC 3 cut(s) 77, 631, 632
SchI GAGTC 3 cut(s) 50, 252, 530
ScrFI CCNGG 2 cut(s) 112, 852
SduI GDGCHC 2 cut(s) 147, 635
SfaNI GCATC 2 cut(s) 553, 761
SfcI CTRYAG 2 cut(s) 393, 895
SinI GGWCC 1 cut(s) 77
SphI GCATGC 3 cut(s) 154, 158, 848
Sse9I AATT 7 cut(s) 24, 294, 424, 498, 544, 763, 804
SsiI CCGC 1 cut(s) 924
SspMI CTAG 3 cut(s) 60, 222, 467
StyD4I CCNGG 2 cut(s) 110, 850
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 68
TaqI TCGA 2 cut(s) 348, 904
TasI AATT 7 cut(s) 24, 294, 424, 498, 544, 763, 804
TatI WGTACW 1 cut(s) 554
TfiI GAWTC 4 cut(s) 218, 518, 623, 749
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TscAI CASTG 3 cut(s) 14, 633, 904
TseFI GTSAC 2 cut(s) 755, 871
Tsp45I GTSAC 2 cut(s) 755, 871
TspDTI ATGAA 2 cut(s) 187, 347
TspGWI ACGGA 1 cut(s) 132
TspRI CASTG 3 cut(s) 14, 633, 904
VpaK11BI GGWCC 1 cut(s) 77
XapI RAATTY 2 cut(s) 294, 804
XbaI TCTAGA 1 cut(s) 221
XceI RCATGY 5 cut(s) 154, 158, 344, 778, 848
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XspI CTAG 3 cut(s) 60, 222, 467
Zsp2I ATGCAT 2 cut(s) 156, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.