Rh2AG324900

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001204
Physical Location & Seq
Reverse (-)
46724754 .. 46726918
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG324900.1

Sequence Viewer

Length: 690 bp
ATGTTGCTTTACAAGCAGAAGCCCAAGGAAACCAAAGGCGACAATATGATGCTGATCAGCATCAGTGTGGTGGGCAGTACTGGCCCGTTCCGGTTCGTCGTGAATAAGAAGGAACTAGTCGCTGCGGTCCTCCAAAGGGCCTTGAAGTCCTACGCGCGCTTAGGTCGGCTACCAGTTCTTGGCTCCGATCTCAAAGAGTTCGTCCTTTACTGCTCCACTGCCGCCGGCGTAGATGCTCTGAGTCCTTGGGAGACAATTGGATCCCGAGGGGCAGTTAATAACTTCATTCTATGCAAGAAGCCACCTACGACTCAGCCACTACCATTGGCTGCAGCTTCTGATCAGAAGAAGAGTCTGATCAGGGGTGATAGGTCATTCTCGGATTCCGGAGCTTTTCATTTTGCTTGTACTCAGTTATGGAAATTGTTAGGATTTTGGTTAAGCATTTGCGAAATTGAACATATTGATTTACTGGAATTGCTTGATTTTGATGGCCTTATCTCAACGAAGCTTCGCTCAGTATCTCCGGCAACGATCCGATTGGCTTCTCTCTGCCGAATAGAGGTAGTCCCTGTTACATTGGCTGGTCTAATTCCTCTAGAGCTTTGTGGTTGCTCTGAAGATGGAAGGAGGTCAGCACTCCAAGATGCATTGCTCTTCCATTCACCAAGGTTATCAACTAAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

25.01

Weight (kDa)

9.11

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 15 - 98 5.1e-19 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 179
AccII CGCG 2 cut(s) 155, 157
AccIII TCCGGA 1 cut(s) 386
AciI CCGC 2 cut(s) 125, 222
AclWI GGATC 3 cut(s) 255, 268, 529
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 2 cut(s) 79, 409
AfiI CCNNNNNNNGG 3 cut(s) 136, 179, 562
AgsI TTSAA 2 cut(s) 145, 458
AhlI ACTAGT 1 cut(s) 115
AluBI AGCT 4 cut(s) 335, 392, 511, 604
AluI AGCT 4 cut(s) 335, 392, 511, 604
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 3 cut(s) 255, 268, 529
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 264
Aor13HI TCCGGA 1 cut(s) 386
AoxI GGCC 3 cut(s) 82, 138, 493
ApeKI GCWGC 3 cut(s) 122, 329, 332
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AspLEI GCGC 2 cut(s) 157, 159
AspS9I GGNCC 3 cut(s) 83, 127, 138
AsuHPI GGTGA 2 cut(s) 377, 657
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 1 cut(s) 127
BamHI GGATCC 1 cut(s) 260
BbvI GCAGC 3 cut(s) 109, 316, 344
BccI CCATC 2 cut(s) 485, 617
BclI TGATCA 3 cut(s) 54, 340, 357
BcoDI GTCTC 1 cut(s) 245
BcuI ACTAGT 1 cut(s) 115
BfaI CTAG 3 cut(s) 116, 599, 688
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 4 cut(s) 123, 222, 330, 333
BlsI GCNGC 4 cut(s) 124, 223, 331, 334
BmcAI AGTACT 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 264
BmgT120I GGNCC 3 cut(s) 83, 127, 138
BmiI GGNNCC 2 cut(s) 184, 262
BmsI GCATC 4 cut(s) 39, 69, 223, 637
Bpu10I CCTNAGC 1 cut(s) 160
BsaBI GATNNNNATC 2 cut(s) 53, 59
BsaJI CCNNGG 4 cut(s) 24, 245, 265, 668
BsaWI WCCGGW 2 cut(s) 90, 386
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 179, 562
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 3 cut(s) 85, 173, 477
Bse3DI GCAATG 1 cut(s) 650
Bse8I GATNNNNATC 2 cut(s) 53, 59
BseAI TCCGGA 1 cut(s) 386
BseDI CCNNGG 4 cut(s) 24, 245, 265, 668
BseJI GATNNNNATC 2 cut(s) 53, 59
BseLI CCNNNNNNNGG 3 cut(s) 136, 179, 562
BseMI GCAATG 1 cut(s) 650
BseMII CTCAG 4 cut(s) 230, 326, 425, 531
BseNI ACTGG 3 cut(s) 85, 173, 477
BsePI GCGCGC 1 cut(s) 155
BseXI GCAGC 3 cut(s) 109, 316, 344
Bsh1236I CGCG 2 cut(s) 155, 157
BshFI GGCC 3 cut(s) 84, 140, 495
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 4 cut(s) 91, 225, 387, 527
BslFI GGGAC 1 cut(s) 554
BslI CCNNNNNNNGG 3 cut(s) 136, 179, 562
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 554
BsnI GGCC 3 cut(s) 84, 140, 495
BsoBI CYCGRG 1 cut(s) 264
Bsp13I TCCGGA 1 cut(s) 386
Bsp143I GATC 6 cut(s) 54, 187, 260, 340, 357, 534
BspACI CCGC 2 cut(s) 125, 222
BspANI GGCC 3 cut(s) 84, 140, 495
BspCNI CTCAG 4 cut(s) 231, 325, 424, 530
BspEI TCCGGA 1 cut(s) 386
BspFNI CGCG 2 cut(s) 155, 157
BspLI GGNNCC 2 cut(s) 184, 262
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 3 cut(s) 255, 268, 529
BspQI GCTCTTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 650
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 3 cut(s) 85, 173, 477
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 4 cut(s) 24, 245, 265, 668
BssHII GCGCGC 1 cut(s) 155
BssMI GATC 6 cut(s) 54, 187, 260, 340, 357, 534
BssT1I CCWWGG 3 cut(s) 24, 245, 668
Bst6I CTCTTC 2 cut(s) 344, 662
BstC8I GCNNGC 2 cut(s) 157, 226
BstDEI CTNAG 5 cut(s) 160, 239, 312, 411, 517
BstFNI CGCG 2 cut(s) 155, 157
BstHHI GCGC 2 cut(s) 157, 159
BstKTI GATC 6 cut(s) 57, 190, 263, 343, 360, 537
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 6 cut(s) 54, 187, 260, 340, 357, 534
BstMWI GCNNNNNNNGC 2 cut(s) 13, 81
BstSFI CTRYAG 1 cut(s) 330
BstUI CGCG 2 cut(s) 155, 157
BstV1I GCAGC 3 cut(s) 109, 316, 344
BstX2I RGATCY 1 cut(s) 260
BstYI RGATCY 1 cut(s) 260
BsuRI GGCC 3 cut(s) 84, 140, 495
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 70, 216
Cac8I GCNNGC 2 cut(s) 157, 226
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 2 cut(s) 157, 159
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 3 cut(s) 83, 127, 138
Csp6I GTAC 2 cut(s) 78, 408
CspCI CAANNNNNGTGG 2 cut(s) 306, 341
CviQI GTAC 2 cut(s) 78, 408
DdeI CTNAG 5 cut(s) 160, 239, 312, 411, 517
DpnI GATC 6 cut(s) 56, 189, 262, 342, 359, 536
DpnII GATC 6 cut(s) 54, 187, 260, 340, 357, 534
Eam1104I CTCTTC 2 cut(s) 344, 662
EarI CTCTTC 2 cut(s) 344, 662
Eco130I CCWWGG 3 cut(s) 24, 245, 668
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 1 cut(s) 264
EcoO109I RGGNCCY 1 cut(s) 138
EcoT14I CCWWGG 3 cut(s) 24, 245, 668
EcoT22I ATGCAT 1 cut(s) 652
ErhI CCWWGG 3 cut(s) 24, 245, 668
FaiI YATR 4 cut(s) 47, 292, 418, 462
FaqI GGGAC 1 cut(s) 554
FbaI TGATCA 3 cut(s) 54, 340, 357
Fnu4HI GCNGC 4 cut(s) 123, 222, 330, 333
Fsp4HI GCNGC 4 cut(s) 123, 222, 330, 333
FspBI CTAG 3 cut(s) 116, 599, 688
GlaI GCGC 2 cut(s) 156, 158
GluI GCNGC 4 cut(s) 123, 222, 330, 333
HaeIII GGCC 3 cut(s) 84, 140, 495
HapII CCGG 4 cut(s) 91, 225, 387, 527
HhaI GCGC 2 cut(s) 157, 159
Hin6I GCGC 2 cut(s) 155, 157
HinP1I GCGC 2 cut(s) 155, 157
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 4 cut(s) 241, 310, 352, 383
HpaII CCGG 4 cut(s) 91, 225, 387, 527
HphI GGTGA 2 cut(s) 377, 657
Hpy188I TCNGA 8 cut(s) 187, 240, 340, 345, 357, 382, 539, 619
Hpy188III TCNNGA 4 cut(s) 100, 264, 387, 599
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 103, 621
HpyCH4V TGCA 3 cut(s) 294, 332, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 81
HpyF3I CTNAG 5 cut(s) 160, 239, 312, 411, 517
HspAI GCGC 2 cut(s) 155, 157
Kpn2I TCCGGA 1 cut(s) 386
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 3 cut(s) 54, 340, 357
Kzo9I GATC 6 cut(s) 54, 187, 260, 340, 357, 534
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 3 cut(s) 188, 218, 389
Lsp1109I GCAGC 3 cut(s) 109, 316, 344
LweI GCATC 4 cut(s) 39, 69, 223, 637
MaeI CTAG 3 cut(s) 116, 599, 688
MaeIII GTNAC 1 cut(s) 574
MalI GATC 6 cut(s) 56, 189, 262, 342, 359, 536
MboI GATC 6 cut(s) 54, 187, 260, 340, 357, 534
MboII GAAGA 4 cut(s) 358, 361, 632, 649
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 1 cut(s) 260
MluCI AATT 5 cut(s) 255, 422, 453, 476, 591
MlyI GAGTC 3 cut(s) 250, 304, 361
MnlI CCTC 5 cut(s) 140, 260, 556, 606, 624
Mph1103I ATGCAT 1 cut(s) 652
MreI CGCCGGCG 1 cut(s) 224
MroI TCCGGA 1 cut(s) 386
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 2 cut(s) 276, 440
MslI CAYNNNNRTG 1 cut(s) 65
MspI CCGG 4 cut(s) 91, 225, 387, 527
MunI CAATTG 1 cut(s) 255
MvnI CGCG 2 cut(s) 155, 157
MwoI GCNNNNNNNGC 2 cut(s) 13, 81
NaeI GCCGGC 1 cut(s) 226
NdeII GATC 6 cut(s) 54, 187, 260, 340, 357, 534
NgoMIV GCCGGC 1 cut(s) 224
NlaIV GGNNCC 2 cut(s) 184, 262
NsiI ATGCAT 1 cut(s) 652
PauI GCGCGC 1 cut(s) 155
PciSI GCTCTTC 1 cut(s) 662
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 1 cut(s) 383
PflMI CCANNNNNTGG 1 cut(s) 179
PkrI GCNGC 4 cut(s) 124, 223, 331, 334
PleI GAGTC 3 cut(s) 249, 304, 360
PpsI GAGTC 3 cut(s) 249, 304, 360
PspN4I GGNNCC 2 cut(s) 184, 262
PspPI GGNCC 3 cut(s) 83, 127, 138
PstI CTGCAG 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 1 cut(s) 260
PteI GCGCGC 1 cut(s) 155
RsaI GTAC 2 cut(s) 79, 409
RsaNI GTAC 2 cut(s) 78, 408
RseI CAYNNNNRTG 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 662
SaqAI TTAA 2 cut(s) 276, 440
SatI GCNGC 4 cut(s) 123, 222, 330, 333
Sau3AI GATC 6 cut(s) 54, 187, 260, 340, 357, 534
Sau96I GGNCC 3 cut(s) 83, 127, 138
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 3 cut(s) 250, 304, 361
SfaNI GCATC 4 cut(s) 39, 69, 223, 637
SfcI CTRYAG 1 cut(s) 330
SgrAI CRCCGGYG 1 cut(s) 224
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 65
SpeI ACTAGT 1 cut(s) 115
Sse9I AATT 5 cut(s) 255, 422, 453, 476, 591
SsiI CCGC 2 cut(s) 125, 222
SspMI CTAG 3 cut(s) 116, 599, 688
StyI CCWWGG 3 cut(s) 24, 245, 668
TasI AATT 5 cut(s) 255, 422, 453, 476, 591
TatI WGTACW 2 cut(s) 77, 407
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 2 cut(s) 276, 440
Tru9I TTAA 2 cut(s) 276, 440
TscAI CASTG 2 cut(s) 70, 223
TseI GCWGC 3 cut(s) 122, 329, 332
TspDTI ATGAA 2 cut(s) 274, 386
TspRI CASTG 2 cut(s) 70, 223
Van91I CCANNNNNTGG 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 127
XbaI TCTAGA 1 cut(s) 598
XspI CTAG 3 cut(s) 116, 599, 688
ZrmI AGTACT 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.