pycom16g12360

calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001204
Physical Location & Seq
Forward (+)
8673383 .. 8675413
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g12360.2

Sequence Viewer

Length: 1269 bp
ATGCAAGGCAACAGTGAAAGTGATTGGGAAACAATTGATGGGGCTTCTGTGTCATGCAAAGTGATTGATCACTTGAGCCGTAGGGATGCCTTGATTCAGAATTTGTTGAAGGATAGACTTTGTGAATGGCTAGTCTCTAAAATTCATGGAGGAGGAAAAGGACCACATGTTCTGGATAATGAAGGCCAAGGTGTTCTACATTTGACGGCTGCTCTTGGTTACGAGTGGGCCATAGGTCCCATAATTGCTGCAGGCGTCAGTCCCAATTTCAGAGATGCTCGCGGCAGAACAGGGCTTCACTGGGCATCATATTTTGGAAGAGAGGAAATTGTCATCACGTTACTTCAACTAGGTGCCGCTCCAGGAGCCGTCGAGGACCCAACGTCAATATTTCCTGGCGGACAAACTGCTGCTGATCTAGCCTCAAGTAGAGGGCATAAGGGGATTGCTGGATATTTGGCAGAAGCAGATTTAACCAGTCACCTAGAGTTATTGACCATGAATGAGAACATAATGAACAATGTTACCGCAACTATTGCAGCTGAGAACGCCAATCAGACTGCAGAAGTTGCTGCATCTGATCCAGTAGATGAGCAACATTCTCTCAAAAACCCTATAGCAGCTGTTAGGAAATCAGCTCATGCGGCTGCTCTAATTCAAGAAGCTTTCCGGGCTCGTTCATTCCGTCATAGACAGTTAACCAAGAGTAGAACAGATGTTTCTGAAGTTCCGTCTCAGGACCTTGTTGCACGTCGTTCCTTGAAGAGAGTACAGAAGTTCGCTCATTATGAAGATTATCTGCATGTTGCAGCAGCTCTGAAGATTCAAAAAAACTATCGTGGATGGAAGGGAAGAAAGGATTATCTGAAGATTCGTGACCGTATTGTTAAAATTCAGGCTCATGTGAGGGGACATCAGGTCCGGAAGAATTACAAAAAGGTTTTGTGGTCTGTTGGTGTATTGGAAAAGGTTATACTGCGTTGGAGGCGAAGAGGAACTGGTCTGCGAGGATTTCAGGTGGAAAAGCCTACTGAAGATGCATCCTCAGAGGTTAAAAAAAATGATGACTATGAATTTCTTAGTGTCGGTCGGAAGCAGAAATTAGCTGGGGTTGAGAAAGCTCTTGCAAGGGTACAGTCCATGGCTCGTCAGCCTGAAGCTTGTGAACAGTATACGAGGCTAATTTCAAACTTTGAGAAACTTGAGATGGCTGACGGGAAGAACCAGGCCTCGAATCAAAATGAAAGTTCCGACCAAAAAGTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 CG-1 IQ TIG

Protein Analysis

423

Amino Acids

46.91

Weight (kDa)

9.46

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1259
AccB1I GGYRCC 1 cut(s) 353
AccBSI CCGCTC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 1172
AccII CGCG 1 cut(s) 282
AccIII TCCGGA 1 cut(s) 921
AciI CCGC 5 cut(s) 282, 357, 399, 528, 644
AclWI GGATC 1 cut(s) 575
AcsI RAATTY 4 cut(s) 100, 141, 891, 1073
AcuI CTGAAG 5 cut(s) 744, 839, 887, 1053, 1176
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 2 cut(s) 771, 1134
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 6 cut(s) 109, 347, 659, 763, 827, 1188
AhdI GACNNNNNGTC 2 cut(s) 382, 917
AjiI CACGTC 1 cut(s) 752
AjnI CCWGG 3 cut(s) 361, 394, 1224
AloI GAACNNNNNNTCC 2 cut(s) 153, 185
AluBI AGCT 8 cut(s) 542, 623, 638, 665, 815, 1106, 1121, 1160
AluI AGCT 8 cut(s) 542, 623, 638, 665, 815, 1106, 1121, 1160
Alw26I GTCTC 2 cut(s) 139, 738
AlwI GGATC 1 cut(s) 575
Aor13HI TCCGGA 1 cut(s) 921
AoxI GGCC 3 cut(s) 184, 228, 1227
ApeKI GCWGC 9 cut(s) 209, 248, 410, 539, 572, 620, 647, 809, 812
ApoI RAATTY 4 cut(s) 100, 141, 891, 1073
AspS9I GGNCC 6 cut(s) 161, 228, 236, 376, 739, 919
AsuC2I CCSGG 1 cut(s) 671
AsuHPI GGTGA 1 cut(s) 473
AvaII GGWCC 5 cut(s) 161, 236, 376, 739, 919
BanI GGYRCC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 676
BbvI GCAGC 9 cut(s) 196, 235, 397, 551, 559, 632, 634, 821, 824
BccI CCATC 3 cut(s) 32, 837, 1201
BceAI ACGGC 3 cut(s) 63, 222, 353
BciT130I CCWGG 3 cut(s) 363, 396, 1226
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 1 cut(s) 671
BcoDI GTCTC 2 cut(s) 139, 738
BfaI CTAG 4 cut(s) 131, 350, 419, 485
BfmI CTRYAG 3 cut(s) 249, 561, 615
Bme1390I CCNGG 4 cut(s) 363, 396, 671, 1226
Bme18I GGWCC 5 cut(s) 161, 236, 376, 739, 919
BmeRI GACNNNNNGTC 2 cut(s) 382, 917
BmgBI CACGTC 1 cut(s) 752
BmgT120I GGNCC 6 cut(s) 161, 228, 236, 376, 739, 919
BmiI GGNNCC 4 cut(s) 238, 355, 367, 378
BmrFI CCNGG 4 cut(s) 363, 396, 671, 1226
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 6 cut(s) 76, 265, 314, 584, 1027, 1049
BmuI ACTGGG 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 345
BpuEI CTTGAG 3 cut(s) 94, 409, 1223
BpuMI CCSGG 1 cut(s) 671
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 187, 1140
BsaWI WCCGGW 1 cut(s) 921
Bse1I ACTGG 4 cut(s) 305, 477, 584, 1003
BseAI TCCGGA 1 cut(s) 921
BseBI CCWGG 3 cut(s) 363, 396, 1226
BseDI CCNNGG 2 cut(s) 187, 1140
BseGI GGATG 3 cut(s) 91, 848, 1040
BseMII CTCAG 3 cut(s) 534, 749, 1059
BseNI ACTGG 4 cut(s) 305, 477, 584, 1003
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 9 cut(s) 196, 235, 397, 551, 559, 632, 634, 821, 824
BseYI CCCAGC 1 cut(s) 1106
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 1090
BshFI GGCC 3 cut(s) 186, 230, 1229
BshNI GGYRCC 1 cut(s) 353
BsiEI CGRYCG 1 cut(s) 1090
BsiSI CCGG 2 cut(s) 670, 922
BslFI GGGAC 3 cut(s) 222, 246, 924
BsmAI GTCTC 2 cut(s) 139, 738
BsmBI CGTCTC 1 cut(s) 738
BsmFI GGGAC 3 cut(s) 222, 246, 924
BsnI GGCC 3 cut(s) 186, 230, 1229
Bsp1286I GDGCHC 1 cut(s) 676
Bsp13I TCCGGA 1 cut(s) 921
Bsp143I GATC 3 cut(s) 67, 415, 580
Bsp19I CCATGG 1 cut(s) 1140
BspACI CCGC 5 cut(s) 282, 357, 399, 528, 644
BspANI GGCC 3 cut(s) 186, 230, 1229
BspCNI CTCAG 3 cut(s) 535, 748, 1058
BspEI TCCGGA 1 cut(s) 921
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 4 cut(s) 238, 355, 367, 378
BspMAI CTGCAG 2 cut(s) 253, 565
BspPI GGATC 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 353
BsrBI CCGCTC 1 cut(s) 359
BsrI ACTGG 4 cut(s) 305, 477, 584, 1003
BssECI CCNNGG 2 cut(s) 187, 1140
BssMI GATC 3 cut(s) 67, 415, 580
BssNAI GTATAC 1 cut(s) 1173
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 2 cut(s) 187, 1140
Bst1107I GTATAC 1 cut(s) 1173
Bst2UI CCWGG 3 cut(s) 363, 396, 1226
Bst4CI ACNGT 5 cut(s) 14, 696, 881, 1137, 1170
Bst6I CTCTTC 3 cut(s) 313, 758, 985
BstACI GRCGYC 1 cut(s) 255
BstAPI GCANNNNNTGC 2 cut(s) 536, 569
BstC8I GCNNGC 2 cut(s) 253, 280
BstDEI CTNAG 4 cut(s) 543, 735, 1045, 1079
BstDSI CCRYGG 1 cut(s) 1140
BstF5I GGATG 3 cut(s) 91, 848, 1040
BstFNI CGCG 1 cut(s) 282
BstKTI GATC 3 cut(s) 70, 418, 583
BstMAI GTCTC 2 cut(s) 139, 738
BstMBI GATC 3 cut(s) 67, 415, 580
BstMCI CGRYCG 1 cut(s) 1090
BstMWI GCNNNNNNNGC 8 cut(s) 365, 419, 536, 548, 569, 644, 671, 985
BstNI CCWGG 3 cut(s) 363, 396, 1226
BstNSI RCATGY 2 cut(s) 170, 806
BstSCI CCNGG 4 cut(s) 361, 394, 669, 1224
BstSFI CTRYAG 3 cut(s) 249, 561, 615
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 9 cut(s) 196, 235, 397, 551, 559, 632, 634, 821, 824
BstZ17I GTATAC 1 cut(s) 1173
BsuRI GGCC 3 cut(s) 186, 230, 1229
BtgI CCRYGG 1 cut(s) 1140
BtrI CACGTC 1 cut(s) 752
BtsCI GGATG 3 cut(s) 91, 848, 1040
BtsIMutI CAGTG 2 cut(s) 19, 298
Cac8I GCNNGC 2 cut(s) 253, 280
Cfr13I GGNCC 6 cut(s) 161, 228, 236, 376, 739, 919
CseI GACGC 1 cut(s) 244
Csp6I GTAC 2 cut(s) 770, 1133
CviAII CATG 8 cut(s) 54, 146, 167, 499, 641, 803, 902, 1141
CviQI GTAC 2 cut(s) 770, 1133
DdeI CTNAG 4 cut(s) 543, 735, 1045, 1079
DpnI GATC 3 cut(s) 69, 417, 582
DpnII GATC 3 cut(s) 67, 415, 580
DrdI GACNNNNNNGTC 1 cut(s) 1259
DriI GACNNNNNGTC 2 cut(s) 382, 917
DseDI GACNNNNNNGTC 1 cut(s) 1259
Eam1104I CTCTTC 3 cut(s) 313, 758, 985
Eam1105I GACNNNNNGTC 2 cut(s) 382, 917
EarI CTCTTC 3 cut(s) 313, 758, 985
EciI GGCGGA 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 187, 1140
Eco147I AGGCCT 1 cut(s) 1229
Eco24I GRGCYC 1 cut(s) 676
Eco47I GGWCC 5 cut(s) 161, 236, 376, 739, 919
Eco57I CTGAAG 5 cut(s) 744, 839, 887, 1053, 1176
EcoO109I RGGNCCY 3 cut(s) 236, 376, 739
EcoRII CCWGG 3 cut(s) 361, 394, 1224
EcoT14I CCWWGG 2 cut(s) 187, 1140
EcoT22I ATGCAT 1 cut(s) 1042
EcoT38I GRGCYC 1 cut(s) 676
ErhI CCWWGG 2 cut(s) 187, 1140
Esp3I CGTCTC 1 cut(s) 738
FaeI CATG 8 cut(s) 57, 149, 170, 502, 644, 806, 905, 1144
FaqI GGGAC 3 cut(s) 222, 246, 924
FatI CATG 8 cut(s) 53, 145, 166, 498, 640, 802, 901, 1140
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 1172
FokI GGATG 3 cut(s) 98, 855, 1027
FriOI GRGCYC 1 cut(s) 676
FspBI CTAG 4 cut(s) 131, 350, 419, 485
GsaI CCCAGC 1 cut(s) 1110
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 3 cut(s) 186, 230, 1229
HapII CCGG 2 cut(s) 670, 922
HgaI GACGC 1 cut(s) 244
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 8 cut(s) 57, 149, 170, 502, 644, 806, 905, 1144
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 2 cut(s) 663, 1158
HinfI GANTC 4 cut(s) 94, 823, 871, 1234
HpaI GTTAAC 1 cut(s) 699
HpaII CCGG 2 cut(s) 670, 922
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 3 cut(s) 699, 1166, 1173
Hpy188III TCNNGA 5 cut(s) 173, 659, 737, 875, 922
Hpy8I GTNNAC 3 cut(s) 699, 1166, 1173
Hpy99I CGWCG 2 cut(s) 374, 756
HpyAV CCTTC 3 cut(s) 103, 176, 841
HpyCH4III ACNGT 5 cut(s) 14, 696, 881, 1137, 1170
HpyCH4IV ACGT 3 cut(s) 338, 383, 751
HpyF10VI GCNNNNNNNGC 8 cut(s) 365, 419, 536, 548, 569, 644, 671, 985
HpyF3I CTNAG 4 cut(s) 543, 735, 1045, 1079
HpySE526I ACGT 3 cut(s) 338, 383, 751
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 8 cut(s) 57, 149, 170, 502, 644, 806, 905, 1144
Kpn2I TCCGGA 1 cut(s) 921
Ksp22I TGATCA 1 cut(s) 67
KspAI GTTAAC 1 cut(s) 699
Kzo9I GATC 3 cut(s) 67, 415, 580
LmnI GCTCC 2 cut(s) 364, 365
Lsp1109I GCAGC 9 cut(s) 196, 235, 397, 551, 559, 632, 634, 821, 824
LweI GCATC 6 cut(s) 76, 265, 314, 584, 1027, 1049
MaeI CTAG 4 cut(s) 131, 350, 419, 485
MaeII ACGT 3 cut(s) 338, 383, 751
MaeIII GTNAC 5 cut(s) 218, 339, 479, 523, 875
MalI GATC 3 cut(s) 69, 417, 582
MbiI CCGCTC 1 cut(s) 359
MboI GATC 3 cut(s) 67, 415, 580
MfeI CAATTG 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 676
MmeI TCCRAC 2 cut(s) 962, 1070
Mph1103I ATGCAT 1 cut(s) 1042
MroI TCCGGA 1 cut(s) 921
MseI TTAA 4 cut(s) 473, 698, 888, 1053
MspA1I CMGCKG 2 cut(s) 542, 623
MspI CCGG 2 cut(s) 670, 922
MspR9I CCNGG 4 cut(s) 363, 396, 671, 1226
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 3 cut(s) 363, 396, 1226
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 8 cut(s) 365, 419, 536, 548, 569, 644, 671, 985
NciI CCSGG 1 cut(s) 671
NcoI CCATGG 1 cut(s) 1140
NdeII GATC 3 cut(s) 67, 415, 580
NlaIII CATG 8 cut(s) 57, 149, 170, 502, 644, 806, 905, 1144
NlaIV GGNNCC 4 cut(s) 238, 355, 367, 378
NmuCI GTSAC 2 cut(s) 479, 875
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 2 cut(s) 170, 806
PceI AGGCCT 1 cut(s) 1229
PciI ACATGT 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 682
PfeI GAWTC 4 cut(s) 94, 823, 871, 1234
PfoI TCCNGGA 1 cut(s) 361
PpuMI RGGWCCY 3 cut(s) 236, 376, 739
PscI ACATGT 1 cut(s) 166
Psp5II RGGWCCY 3 cut(s) 236, 376, 739
Psp6I CCWGG 3 cut(s) 361, 394, 1224
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 3 cut(s) 361, 394, 1224
PspN4I GGNNCC 4 cut(s) 238, 355, 367, 378
PspPI GGNCC 6 cut(s) 161, 228, 236, 376, 739, 919
PspPPI RGGWCCY 3 cut(s) 236, 376, 739
PstI CTGCAG 2 cut(s) 253, 565
PvuII CAGCTG 2 cut(s) 542, 623
RsaI GTAC 2 cut(s) 771, 1134
RsaNI GTAC 2 cut(s) 770, 1133
SaqAI TTAA 4 cut(s) 473, 698, 888, 1053
Sau3AI GATC 3 cut(s) 67, 415, 580
Sau96I GGNCC 6 cut(s) 161, 228, 236, 376, 739, 919
ScrFI CCNGG 4 cut(s) 363, 396, 671, 1226
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 6 cut(s) 76, 265, 314, 584, 1027, 1049
SfcI CTRYAG 3 cut(s) 249, 561, 615
SinI GGWCC 5 cut(s) 161, 236, 376, 739, 919
SmlI CTYRAG 3 cut(s) 73, 424, 1202
SmoI CTYRAG 3 cut(s) 73, 424, 1202
SseBI AGGCCT 1 cut(s) 1229
SsiI CCGC 5 cut(s) 282, 357, 399, 528, 644
SspI AATATT 1 cut(s) 390
SspMI CTAG 4 cut(s) 131, 350, 419, 485
StuI AGGCCT 1 cut(s) 1229
StyD4I CCNGG 4 cut(s) 361, 394, 669, 1224
StyI CCWWGG 2 cut(s) 187, 1140
TaaI ACNGT 5 cut(s) 14, 696, 881, 1137, 1170
TaiI ACGT 3 cut(s) 341, 386, 754
TaqI TCGA 2 cut(s) 372, 1232
TaqII GACCGA 1 cut(s) 1076
TatI WGTACW 1 cut(s) 769
TauI GCSGC 3 cut(s) 285, 359, 647
TfiI GAWTC 4 cut(s) 94, 823, 871, 1234
Tru1I TTAA 4 cut(s) 473, 698, 888, 1053
Tru9I TTAA 4 cut(s) 473, 698, 888, 1053
TscAI CASTG 2 cut(s) 19, 305
TseFI GTSAC 2 cut(s) 479, 875
TseI GCWGC 9 cut(s) 209, 248, 410, 539, 572, 620, 647, 809, 812
Tsp45I GTSAC 2 cut(s) 479, 875
TspDTI ATGAA 8 cut(s) 134, 195, 515, 530, 669, 804, 1086, 1257
TspGWI ACGGA 2 cut(s) 674, 720
TspRI CASTG 2 cut(s) 19, 305
VpaK11BI GGWCC 5 cut(s) 161, 236, 376, 739, 919
XapI RAATTY 4 cut(s) 100, 141, 891, 1073
XceI RCATGY 2 cut(s) 170, 806
XmiI GTMKAC 1 cut(s) 1172
XspI CTAG 4 cut(s) 131, 350, 419, 485
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.