AT5G66370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001391
Physical Location & Seq
Reverse (-)
26512200 .. 26512824
625 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66370.1

Sequence Viewer

Length: 453 bp
ATGTTTTCATCAAATAATCGTTCTACACAACTGACTTGTGGGTTTAAGGTGAACACCAACTCCTCTGAGTGGTATAAGAGCATCACAAGGATTCTAAAGAAGGTCAAAGGAGGGGATTTTTTGTTAGACGCGGACGAAGGAAGAGCATACATTTCGGGTTTAGGAGATCCACATAAACTACTAAAACTAATGGGTTCGGTAAAAGGCAAGGCAGCTGAAATGACGTTTGTGAAAACCGGTGGACATCATCATCATCATCAGCATCATTATCCACATGAACCTAATCTTCATTACAACTCCGACTCCTATTACGGCCAGCCACCATCTTACAACAGTTATTGGCCGAGTGATAATTGTTACTCTCGCCAGCATCCGCCTTATTATTCTCAGTCACTAGCGATGCAACCTTACCATCACCAATATCCATATCCAGGTTATAGTATCTACGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

17.26

Weight (kDa)

8.94

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 131
AciI CCGC 2 cut(s) 131, 374
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 2 cut(s) 313, 341
AfiI CCNNNNNNNGG 2 cut(s) 69, 431
AgeI ACCGGT 1 cut(s) 236
AjnI CCWGG 1 cut(s) 430
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 1 cut(s) 215
AluI AGCT 1 cut(s) 215
AlwI GGATC 1 cut(s) 161
AoxI GGCC 2 cut(s) 313, 341
ApeKI GCWGC 1 cut(s) 212
AsiGI ACCGGT 1 cut(s) 236
AsuHPI GGTGA 2 cut(s) 61, 407
BbvI GCAGC 1 cut(s) 224
BccI CCATC 2 cut(s) 331, 420
BceAI ACGGC 1 cut(s) 328
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BciT130I CCWGG 1 cut(s) 432
BfaI CTAG 1 cut(s) 395
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 432
BmrFI CCNGG 1 cut(s) 432
BmsI GCATC 4 cut(s) 90, 271, 379, 390
BsaWI WCCGGW 1 cut(s) 236
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 287, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 431
Bse118I RCCGGY 1 cut(s) 236
BseBI CCWGG 1 cut(s) 432
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 2 cut(s) 69, 431
BseMII CTCAG 2 cut(s) 57, 401
BseRI GAGGAG 1 cut(s) 52
BseXI GCAGC 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 2 cut(s) 315, 343
BshTI ACCGGT 1 cut(s) 236
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 69, 431
BsnI GGCC 2 cut(s) 315, 343
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 2 cut(s) 131, 374
BspANI GGCC 2 cut(s) 315, 343
BspCNI CTCAG 2 cut(s) 58, 400
BspFNI CGCG 1 cut(s) 131
BspPI GGATC 1 cut(s) 161
BspQI GCTCTTC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 236
BssMI GATC 1 cut(s) 166
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 2 cut(s) 335, 449
Bst6I CTCTTC 1 cut(s) 136
BstC8I GCNNGC 2 cut(s) 317, 368
BstDEI CTNAG 2 cut(s) 66, 387
BstF5I GGATG 1 cut(s) 370
BstFNI CGCG 1 cut(s) 131
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 432
BstSCI CCNGG 1 cut(s) 430
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 224
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 2 cut(s) 315, 343
BtgZI GCGATG 1 cut(s) 413
BtsCI GGATG 1 cut(s) 370
Cac8I GCNNGC 2 cut(s) 317, 368
Cfr10I RCCGGY 1 cut(s) 236
CseI GACGC 1 cut(s) 137
CspAI ACCGGT 1 cut(s) 236
CviAII CATG 1 cut(s) 275
CviJI RGCY 4 cut(s) 215, 315, 319, 343
CviKI_1 RGCY 4 cut(s) 215, 315, 319, 343
DdeI CTNAG 2 cut(s) 66, 387
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
EaeI YGGCCR 2 cut(s) 313, 341
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
EciI GGCGGA 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 430
FaeI CATG 1 cut(s) 278
FaiI YATR 6 cut(s) 75, 148, 174, 276, 427, 438
FatI CATG 1 cut(s) 274
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 1 cut(s) 357
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 395
GluI GCNGC 1 cut(s) 213
HaeIII GGCC 2 cut(s) 315, 343
HapII CCGG 1 cut(s) 237
HgaI GACGC 1 cut(s) 137
Hin1II CATG 1 cut(s) 278
HinfI GANTC 2 cut(s) 91, 302
HpaII CCGG 1 cut(s) 237
HphI GGTGA 2 cut(s) 61, 407
Hpy166II GTNNAC 2 cut(s) 52, 242
Hpy188I TCNGA 2 cut(s) 67, 301
Hpy8I GTNNAC 2 cut(s) 52, 242
HpyAV CCTTC 2 cut(s) 94, 131
HpyCH4III ACNGT 2 cut(s) 335, 449
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 1 cut(s) 403
HpyF3I CTNAG 2 cut(s) 66, 387
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 1 cut(s) 278
Kzo9I GATC 1 cut(s) 166
LguI GCTCTTC 1 cut(s) 136
LpnPI CCDG 5 cut(s) 250, 329, 380, 417, 444
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 4 cut(s) 90, 271, 379, 390
MaeI CTAG 1 cut(s) 395
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 2 cut(s) 356, 390
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 2 cut(s) 153, 278
MflI RGATCY 1 cut(s) 166
MluCI AATT 1 cut(s) 352
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 2 cut(s) 73, 104
MseI TTAA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
MvnI CGCG 1 cut(s) 131
NdeII GATC 1 cut(s) 166
NlaIII CATG 1 cut(s) 278
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 390
PciSI GCTCTTC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 91
PinAI ACCGGT 1 cut(s) 236
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PsuI RGATCY 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 136
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 1 cut(s) 166
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 432
SetI ASST 7 cut(s) 51, 105, 217, 227, 283, 409, 436
SfaNI GCATC 4 cut(s) 90, 271, 379, 390
Sse9I AATT 1 cut(s) 352
SsiI CCGC 2 cut(s) 131, 374
SspMI CTAG 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 430
TaaI ACNGT 2 cut(s) 335, 449
TaiI ACGT 1 cut(s) 227
TasI AATT 1 cut(s) 352
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TseFI GTSAC 1 cut(s) 390
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 390
TspDTI ATGAA 2 cut(s) 278, 291
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.