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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 276 |
| AccI | GTMKAC | 1 cut(s) | 19 |
| AciI | CCGC | 1 cut(s) | 384 |
| AcoI | YGGCCR | 1 cut(s) | 385 |
| AfaI | GTAC | 1 cut(s) | 159 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 154, 383 |
| AgsI | TTSAA | 1 cut(s) | 239 |
| AluBI | AGCT | 3 cut(s) | 15, 169, 314 |
| AluI | AGCT | 3 cut(s) | 15, 169, 314 |
| AoxI | GGCC | 1 cut(s) | 385 |
| ApeKI | GCWGC | 1 cut(s) | 311 |
| AsuHPI | GGTGA | 1 cut(s) | 355 |
| BbsI | GAAGAC | 1 cut(s) | 246 |
| BbvI | GCAGC | 1 cut(s) | 323 |
| BccI | CCATC | 2 cut(s) | 42, 375 |
| BciVI | GTATCC | 1 cut(s) | 311 |
| BfmI | CTRYAG | 1 cut(s) | 265 |
| BfuI | GTATCC | 1 cut(s) | 311 |
| BglII | AGATCT | 1 cut(s) | 195 |
| BisI | GCNGC | 2 cut(s) | 312, 385 |
| BlsI | GCNGC | 2 cut(s) | 313, 386 |
| BmsI | GCATC | 1 cut(s) | 60 |
| BpiI | GAAGAC | 1 cut(s) | 246 |
| BsaJI | CCNNGG | 1 cut(s) | 388 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 154, 383 |
| BseDI | CCNNGG | 1 cut(s) | 388 |
| BseGI | GGATG | 1 cut(s) | 53 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 154, 383 |
| BseRI | GAGGAG | 1 cut(s) | 102 |
| BseXI | GCAGC | 1 cut(s) | 323 |
| BseYI | CCCAGC | 1 cut(s) | 179 |
| BshFI | GGCC | 1 cut(s) | 387 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 154, 383 |
| BsnI | GGCC | 1 cut(s) | 387 |
| Bsp143I | GATC | 1 cut(s) | 195 |
| Bsp19I | CCATGG | 1 cut(s) | 388 |
| BspACI | CCGC | 1 cut(s) | 384 |
| BspANI | GGCC | 1 cut(s) | 387 |
| BssECI | CCNNGG | 1 cut(s) | 388 |
| BssMI | GATC | 1 cut(s) | 195 |
| BssT1I | CCWWGG | 1 cut(s) | 388 |
| Bst4CI | ACNGT | 2 cut(s) | 227, 341 |
| BstDSI | CCRYGG | 1 cut(s) | 388 |
| BstF5I | GGATG | 1 cut(s) | 53 |
| BstKTI | GATC | 1 cut(s) | 198 |
| BstMBI | GATC | 1 cut(s) | 195 |
| BstSFI | CTRYAG | 1 cut(s) | 265 |
| BstV1I | GCAGC | 1 cut(s) | 323 |
| BstV2I | GAAGAC | 1 cut(s) | 246 |
| BstX2I | RGATCY | 1 cut(s) | 195 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 395 |
| BstYI | RGATCY | 1 cut(s) | 195 |
| BsuI | GTATCC | 1 cut(s) | 311 |
| BsuRI | GGCC | 1 cut(s) | 387 |
| BtgI | CCRYGG | 1 cut(s) | 388 |
| BtsCI | GGATG | 1 cut(s) | 53 |
| BtsIMutI | CAGTG | 1 cut(s) | 232 |
| Csp6I | GTAC | 1 cut(s) | 158 |
| CviAII | CATG | 1 cut(s) | 389 |
| CviJI | RGCY | 6 cut(s) | 15, 169, 179, 314, 355, 387 |
| CviKI_1 | RGCY | 6 cut(s) | 15, 169, 179, 314, 355, 387 |
| CviQI | GTAC | 1 cut(s) | 158 |
| DpnI | GATC | 1 cut(s) | 197 |
| DpnII | GATC | 1 cut(s) | 195 |
| DraI | TTTAAA | 1 cut(s) | 105 |
| EaeI | YGGCCR | 1 cut(s) | 385 |
| Eco130I | CCWWGG | 1 cut(s) | 388 |
| EcoT14I | CCWWGG | 1 cut(s) | 388 |
| ErhI | CCWWGG | 1 cut(s) | 388 |
| FaeI | CATG | 1 cut(s) | 392 |
| FatI | CATG | 1 cut(s) | 388 |
| FblI | GTMKAC | 1 cut(s) | 19 |
| Fnu4HI | GCNGC | 2 cut(s) | 312, 385 |
| FokI | GGATG | 1 cut(s) | 60 |
| Fsp4HI | GCNGC | 2 cut(s) | 312, 385 |
| GluI | GCNGC | 2 cut(s) | 312, 385 |
| GsaI | CCCAGC | 1 cut(s) | 183 |
| HaeIII | GGCC | 1 cut(s) | 387 |
| Hin1II | CATG | 1 cut(s) | 392 |
| HincII | GTYRAC | 1 cut(s) | 31 |
| HindII | GTYRAC | 1 cut(s) | 31 |
| HphI | GGTGA | 1 cut(s) | 355 |
| Hpy166II | GTNNAC | 4 cut(s) | 20, 31, 145, 298 |
| Hpy188I | TCNGA | 1 cut(s) | 86 |
| Hpy8I | GTNNAC | 4 cut(s) | 20, 31, 145, 298 |
| Hpy99I | CGWCG | 1 cut(s) | 154 |
| HpyAV | CCTTC | 3 cut(s) | 110, 148, 169 |
| HpyCH4III | ACNGT | 2 cut(s) | 227, 341 |
| HpyCH4V | TGCA | 2 cut(s) | 208, 311 |
| Hsp92II | CATG | 1 cut(s) | 392 |
| Kzo9I | GATC | 1 cut(s) | 195 |
| LmnI | GCTCC | 2 cut(s) | 89, 129 |
| LpnPI | CCDG | 5 cut(s) | 27, 74, 120, 165, 300 |
| Lsp1109I | GCAGC | 1 cut(s) | 323 |
| LweI | GCATC | 1 cut(s) | 60 |
| MaeIII | GTNAC | 1 cut(s) | 217 |
| MalI | GATC | 1 cut(s) | 197 |
| MboI | GATC | 1 cut(s) | 195 |
| MboII | GAAGA | 3 cut(s) | 251, 321, 324 |
| MflI | RGATCY | 1 cut(s) | 195 |
| MluCI | AATT | 1 cut(s) | 289 |
| MnlI | CCTC | 1 cut(s) | 80 |
| MseI | TTAA | 1 cut(s) | 104 |
| MslI | CAYNNNNRTG | 1 cut(s) | 393 |
| MspA1I | CMGCKG | 1 cut(s) | 314 |
| NcoI | CCATGG | 1 cut(s) | 388 |
| NdeII | GATC | 1 cut(s) | 195 |
| NlaIII | CATG | 1 cut(s) | 392 |
| PflFI | GACNNNGTC | 1 cut(s) | 227 |
| PkrI | GCNGC | 2 cut(s) | 313, 386 |
| PsiI | TTATAA | 1 cut(s) | 276 |
| PspFI | CCCAGC | 1 cut(s) | 179 |
| PsuI | RGATCY | 1 cut(s) | 195 |
| PsyI | GACNNNGTC | 1 cut(s) | 227 |
| PvuII | CAGCTG | 1 cut(s) | 314 |
| RsaI | GTAC | 1 cut(s) | 159 |
| RsaNI | GTAC | 1 cut(s) | 158 |
| RseI | CAYNNNNRTG | 1 cut(s) | 393 |
| SaqAI | TTAA | 1 cut(s) | 104 |
| SatI | GCNGC | 2 cut(s) | 312, 385 |
| Sau3AI | GATC | 1 cut(s) | 195 |
| SetI | ASST | 6 cut(s) | 17, 159, 171, 251, 316, 327 |
| SfaNI | GCATC | 1 cut(s) | 60 |
| SfcI | CTRYAG | 1 cut(s) | 265 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 393 |
| Sse9I | AATT | 1 cut(s) | 289 |
| SsiI | CCGC | 1 cut(s) | 384 |
| StyI | CCWWGG | 1 cut(s) | 388 |
| TaaI | ACNGT | 2 cut(s) | 227, 341 |
| TasI | AATT | 1 cut(s) | 289 |
| TauI | GCSGC | 1 cut(s) | 387 |
| Tru1I | TTAA | 1 cut(s) | 104 |
| Tru9I | TTAA | 1 cut(s) | 104 |
| TscAI | CASTG | 1 cut(s) | 232 |
| TseI | GCWGC | 1 cut(s) | 311 |
| TspDTI | ATGAA | 1 cut(s) | 324 |
| TspGWI | ACGGA | 1 cut(s) | 116 |
| TspRI | CASTG | 1 cut(s) | 232 |
| Tth111I | GACNNNGTC | 1 cut(s) | 227 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 395 |
| XmiI | GTMKAC | 1 cut(s) | 19 |