Rorug06G0423400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001391
Physical Location & Seq
Forward (+)
56168057 .. 56171492
3436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0423400.1

Sequence Viewer

Length: 1512 bp
ATGGGGAGAAAGAAGAAGTTCATTGACAAGAAAAAGTCGTCTACTTTCCAATTAGTCGCCCGAGACTCCTCCGACCCCAACTACGACGACTCACCCGGTGGCGACCGTGTCTTCATCCAAGTCTCCGGCCGACCTAACGACGATCCCGATTCTATCTTCGCCGACGCGCCTGAGGACGAAGAAGGTGACCACTATTACGACCAGGCTTTCGGAAACTCTGAGGCGGCGGCTCAGCCCCTGCCTGAGAAACTGAGGAAGGAGATTCTGGACCTCGGGTTTCCCGACGACGGTTACAACTATTTGAACCATTTGAGGGAGATTAAGAACACCGGCGGTGGCTCCGCCTATTACTCCAACCCCAAGGCTAAGCTCGAGCAGCTTCCGCATGACGTCAAGGCATATGATGCTTCAAGATTGCGAATTTCGGAGAATGAGGCTCCGGATGAGAATGCTATTTACAATGTGGCGTCTAAGACAGTCAATGTGAGGGTTAAGAAAGCTGTTGATCCGGAAGTTTCTGCGTTGCTTGATGCTGATAGCGATGCTTCGCGGTTCGGTTCTGATGATGAGGATTTGGAAGAGGATTTTGTGGTTAAGGCTAACCTTGCTGATGGAGATGAAGAGGAGGATGATGGGCCAAGTTTTGTTGAGCAGTCGGAGAACAACAATGCAAGGAATGAAGTTGGTGTGGTTGGAAGAAATTGCGTGGAGAGAGGAAAGGGTGACTTTGTGGTAGATGATCCACGAGCTCGTCGGGATGTGGATGAAGAATTTGATATGGTTTTAAGTCGGGAATATGACAATGACGATGATGATGACGACTATGAAGGTGTTGATCATGACGATGATTATTTTTATGGAGATGAAGTGGATGAATCCATTGAGGAGAAGCTCAAGAACGTCAAACTTAATGACTGCGTGAAGGATGACTTTGAGCTTAATGAATATGAGGTTCCGGGAGATGATGAGGAGAAACAGAAAACGAAGCAGCTTGGGAAGATATATGAGGATGAAGATCAAGAGGACAAGGTGGTAATTGTAGAGGAATCTAGTGATGAATCAGAAAAATGGGACTGTGAGACTGTTATTACTACATATTCAAATCTTGAGAACCATCCTGCGAAAATCGGAGCTCCAGAGCTAAGTAGAAAGAAGAAGCAGCTTTCAGAAACCGTTTTTGGAGGCTTGGATGCCTCCAATAATTTGATAAAGCTTGGGGGAAGACAAAGGCTGCCTGTGGACTTCTTGCCTCGTAATAGAAGACCTGCTGCTACAGAGAGGGCGACAGAGAAGGTGGAAGATATAGGTAGCTTGAAAACTGAGCAGCAGAAAAGGAAGCAACATGGTCAGGAGCCAAAGGAGGAGAAGAAAGAGCGGAAGGCGGCTGTCAAGGAGGAAAGACGTGAGGCACGGCGCGCTAAAAAGGAATTGAAGGAACTTTACAAGGGTGAAGCGCAGCATGCTCAGAGAGTAGCTGCTATATCTGGTCCTTCTTCCAAACGTCTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

503

Amino Acids

56.92

Weight (kDa)

4.61

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 393
Acc36I ACCTGC 1 cut(s) 1273
AccBSI CCGCTC 1 cut(s) 1375
AccI GTMKAC 1 cut(s) 41
AccII CGCG 3 cut(s) 167, 550, 1416
AccIII TCCGGA 2 cut(s) 439, 508
AciI CCGC 8 cut(s) 224, 227, 333, 342, 383, 550, 1375, 1382
AclWI GGATC 3 cut(s) 137, 500, 734
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 2 cut(s) 420, 770
AcyI GRCGYC 2 cut(s) 390, 467
AdeI CACNNNGTG 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 287, 313
AgsI TTSAA 5 cut(s) 304, 411, 1101, 1315, 1432
AjiI CACGTC 1 cut(s) 1403
AjnI CCWGG 1 cut(s) 201
AjuI GAANNNNNNNTTGG 2 cut(s) 1161, 1193
Alw21I GWGCWC 2 cut(s) 751, 1135
Alw26I GTCTC 3 cut(s) 57, 127, 1073
AlwI GGATC 3 cut(s) 137, 500, 734
AlwNI CAGNNNCTG 1 cut(s) 238
Ama87I CYCGRG 3 cut(s) 60, 272, 371
Aor13HI TCCGGA 2 cut(s) 439, 508
AoxI GGCC 2 cut(s) 127, 635
ApeKI GCWGC 8 cut(s) 376, 988, 1159, 1231, 1268, 1324, 1456, 1475
ApoI RAATTY 2 cut(s) 420, 770
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 4 cut(s) 169, 1416, 1418, 1456
AspS9I GGNCC 3 cut(s) 268, 635, 1487
AsuC2I CCSGG 2 cut(s) 96, 957
AsuHPI GGTGA 4 cut(s) 84, 197, 734, 1460
AvaI CYCGRG 3 cut(s) 60, 272, 371
AvaII GGWCC 2 cut(s) 268, 1487
AxyI CCTNAGG 1 cut(s) 171
BanII GRGCYC 2 cut(s) 751, 1135
BarI GAAGNNNNNNTAC 2 cut(s) 1424, 1456
BauI CACGAG 1 cut(s) 744
BbsI GAAGAC 3 cut(s) 103, 1228, 1267
Bbv12I GWGCWC 2 cut(s) 751, 1135
BbvI GCAGC 8 cut(s) 388, 1000, 1171, 1218, 1255, 1336, 1462, 1468
BccI CCATC 3 cut(s) 605, 626, 1122
BceAI ACGGC 1 cut(s) 1427
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 835
BcnI CCSGG 2 cut(s) 96, 957
BcoDI GTCTC 3 cut(s) 57, 127, 1073
BfaI CTAG 1 cut(s) 1050
BfmI CTRYAG 1 cut(s) 1272
BfuAI ACCTGC 1 cut(s) 1273
BlpI GCTNAGC 2 cut(s) 231, 366
Bme1390I CCNGG 3 cut(s) 96, 203, 957
Bme18I GGWCC 2 cut(s) 268, 1487
BmeT110I CYCGRG 3 cut(s) 60, 272, 371
BmgBI CACGTC 1 cut(s) 1403
BmgT120I GGNCC 3 cut(s) 268, 635, 1487
BmiI GGNNCC 4 cut(s) 340, 438, 954, 1353
BmrFI CCNGG 3 cut(s) 96, 203, 957
BmsI GCATC 4 cut(s) 394, 520, 532, 1180
BpiI GAAGAC 3 cut(s) 103, 1228, 1267
BpmI CTGGAG 1 cut(s) 1119
Bpu1102I GCTNAGC 2 cut(s) 231, 366
BpuEI CTTGAG 2 cut(s) 878, 1127
BpuMI CCSGG 2 cut(s) 96, 957
BsaBI GATNNNNATC 1 cut(s) 1014
BsaHI GRCGYC 2 cut(s) 390, 467
BsaJI CCNNGG 2 cut(s) 271, 360
BsaWI WCCGGW 2 cut(s) 439, 508
BsaXI ACNNNNNCTCC 2 cut(s) 852, 882
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 313
Bse118I RCCGGY 1 cut(s) 329
Bse21I CCTNAGG 1 cut(s) 171
Bse8I GATNNNNATC 1 cut(s) 1014
BseAI TCCGGA 2 cut(s) 439, 508
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 271, 360
BseJI GATNNNNATC 1 cut(s) 1014
BseLI CCNNNNNNNGG 2 cut(s) 287, 313
BseMII CTCAG 7 cut(s) 162, 210, 234, 242, 245, 1311, 1478
BsePI GCGCGC 1 cut(s) 1414
BseRI GAGGAG 5 cut(s) 58, 638, 899, 983, 1376
BseX3I CGGCCG 1 cut(s) 127
BseXI GCAGC 8 cut(s) 388, 1000, 1171, 1218, 1255, 1336, 1462, 1468
Bsh1236I CGCG 3 cut(s) 167, 550, 1416
Bsh1285I CGRYCG 2 cut(s) 106, 130
BshFI GGCC 2 cut(s) 129, 637
BsiEI CGRYCG 2 cut(s) 106, 130
BsiHKAI GWGCWC 2 cut(s) 751, 1135
BsiHKCI CYCGRG 3 cut(s) 60, 272, 371
BsiSI CCGG 6 cut(s) 96, 126, 330, 440, 509, 956
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 2 cut(s) 287, 313
BsmAI GTCTC 3 cut(s) 57, 127, 1073
BsmFI GGGAC 1 cut(s) 1085
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 2 cut(s) 129, 637
BsoBI CYCGRG 3 cut(s) 60, 272, 371
Bsp1286I GDGCHC 2 cut(s) 751, 1135
Bsp13I TCCGGA 2 cut(s) 439, 508
Bsp143I GATC 5 cut(s) 142, 505, 739, 835, 1015
Bsp1720I GCTNAGC 2 cut(s) 231, 366
BspACI CCGC 8 cut(s) 224, 227, 333, 342, 383, 550, 1375, 1382
BspANI GGCC 2 cut(s) 129, 637
BspCNI CTCAG 7 cut(s) 163, 211, 235, 243, 244, 1312, 1477
BspEI TCCGGA 2 cut(s) 439, 508
BspFNI CGCG 3 cut(s) 167, 550, 1416
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 4 cut(s) 340, 438, 954, 1353
BspMI ACCTGC 1 cut(s) 1273
BspPI GGATC 3 cut(s) 137, 500, 734
BsrBI CCGCTC 1 cut(s) 1375
BsrFI RCCGGY 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 2 cut(s) 271, 360
BssHII GCGCGC 1 cut(s) 1414
BssMI GATC 5 cut(s) 142, 505, 739, 835, 1015
BssNI GRCGYC 2 cut(s) 390, 467
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 1 cut(s) 360
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 6 cut(s) 107, 290, 478, 1076, 1084, 1174
Bst6I CTCTTC 2 cut(s) 573, 615
BstACI GRCGYC 2 cut(s) 390, 467
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 2 cut(s) 1416, 1461
BstEII GGTNACC 1 cut(s) 185
BstFNI CGCG 3 cut(s) 167, 550, 1416
BstHHI GCGC 4 cut(s) 169, 1416, 1418, 1456
BstKTI GATC 5 cut(s) 145, 508, 742, 838, 1018
BstMAI GTCTC 3 cut(s) 57, 127, 1073
BstMBI GATC 5 cut(s) 142, 505, 739, 835, 1015
BstMCI CGRYCG 2 cut(s) 106, 130
BstMWI GCNNNNNNNGC 6 cut(s) 376, 382, 404, 605, 1415, 1460
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 1463
BstPI GGTNACC 1 cut(s) 185
BstSCI CCNGG 3 cut(s) 94, 201, 955
BstSFI CTRYAG 1 cut(s) 1272
BstUI CGCG 3 cut(s) 167, 550, 1416
BstV1I GCAGC 8 cut(s) 388, 1000, 1171, 1218, 1255, 1336, 1462, 1468
BstV2I GAAGAC 3 cut(s) 103, 1228, 1267
BstZI CGGCCG 1 cut(s) 127
Bsu36I CCTNAGG 1 cut(s) 171
BsuRI GGCC 2 cut(s) 129, 637
BtgZI GCGATG 1 cut(s) 555
BtrI CACGTC 1 cut(s) 1403
BveI ACCTGC 1 cut(s) 1273
Cac8I GCNNGC 2 cut(s) 1416, 1461
CaiI CAGNNNCTG 1 cut(s) 238
CciI TCATGA 1 cut(s) 838
CfoI GCGC 4 cut(s) 169, 1416, 1418, 1456
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 3 cut(s) 268, 635, 1487
CseI GACGC 2 cut(s) 173, 456
CviAII CATG 4 cut(s) 386, 839, 1343, 1460
DpnI GATC 5 cut(s) 144, 507, 741, 837, 1017
DpnII GATC 5 cut(s) 142, 505, 739, 835, 1015
DraIII CACNNNGTG 1 cut(s) 98
EaeI YGGCCR 1 cut(s) 127
EagI CGGCCG 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 573, 615
EarI CTCTTC 2 cut(s) 573, 615
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 2 cut(s) 749, 1133
EclXI CGGCCG 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 360
Eco24I GRGCYC 2 cut(s) 751, 1135
Eco47I GGWCC 2 cut(s) 268, 1487
Eco52I CGGCCG 1 cut(s) 127
Eco53kI GAGCTC 2 cut(s) 749, 1133
Eco81I CCTNAGG 1 cut(s) 171
Eco88I CYCGRG 3 cut(s) 60, 272, 371
Eco91I GGTNACC 1 cut(s) 185
EcoICRI GAGCTC 2 cut(s) 749, 1133
EcoO65I GGTNACC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 360
EcoT38I GRGCYC 2 cut(s) 751, 1135
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 4 cut(s) 389, 842, 1346, 1463
FalI AAGNNNNNCTT 4 cut(s) 710, 742, 914, 946
FaqI GGGAC 1 cut(s) 1085
FatI CATG 4 cut(s) 385, 838, 1342, 1459
FauNDI CATATG 1 cut(s) 400
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 1 cut(s) 41
FriOI GRGCYC 2 cut(s) 751, 1135
FspBI CTAG 1 cut(s) 1050
GlaI GCGC 4 cut(s) 168, 1415, 1417, 1455
GsuI CTGGAG 1 cut(s) 1119
HaeIII GGCC 2 cut(s) 129, 637
HapII CCGG 6 cut(s) 96, 126, 330, 440, 509, 956
HgaI GACGC 2 cut(s) 173, 456
HhaI GCGC 4 cut(s) 169, 1416, 1418, 1456
Hin1I GRCGYC 2 cut(s) 390, 467
Hin1II CATG 4 cut(s) 389, 842, 1346, 1463
Hin6I GCGC 4 cut(s) 167, 1414, 1416, 1454
HinP1I GCGC 4 cut(s) 167, 1414, 1416, 1454
HindIII AAGCTT 1 cut(s) 1211
HinfI GANTC 7 cut(s) 65, 89, 149, 262, 875, 1046, 1058
HpaII CCGG 6 cut(s) 96, 126, 330, 440, 509, 956
HphI GGTGA 4 cut(s) 84, 197, 734, 1460
Hpy166II GTNNAC 2 cut(s) 42, 1240
Hpy8I GTNNAC 2 cut(s) 42, 1240
Hpy99I CGWCG 6 cut(s) 89, 143, 167, 287, 290, 756
HpyAV CCTTC 8 cut(s) 176, 250, 821, 916, 1285, 1372, 1426, 1500
HpyCH4III ACNGT 6 cut(s) 107, 290, 478, 1076, 1084, 1174
HpyCH4IV ACGT 4 cut(s) 390, 900, 1402, 1501
HpyCH4V TGCA 1 cut(s) 671
HpyF10VI GCNNNNNNNGC 6 cut(s) 376, 382, 404, 605, 1415, 1460
HpySE526I ACGT 4 cut(s) 390, 900, 1402, 1501
Hsp92I GRCGYC 2 cut(s) 390, 467
Hsp92II CATG 4 cut(s) 389, 842, 1346, 1463
HspAI GCGC 4 cut(s) 167, 1414, 1416, 1454
Kpn2I TCCGGA 2 cut(s) 439, 508
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 5 cut(s) 142, 505, 739, 835, 1015
LmnI GCTCC 5 cut(s) 344, 442, 1130, 1138, 1351
Lsp1109I GCAGC 8 cut(s) 388, 1000, 1171, 1218, 1255, 1336, 1462, 1468
LweI GCATC 4 cut(s) 394, 520, 532, 1180
MaeI CTAG 1 cut(s) 1050
MaeII ACGT 4 cut(s) 390, 900, 1402, 1501
MaeIII GTNAC 3 cut(s) 185, 290, 722
MalI GATC 5 cut(s) 144, 507, 741, 837, 1017
MbiI CCGCTC 1 cut(s) 1375
MboI GATC 5 cut(s) 142, 505, 739, 835, 1015
MhlI GDGCHC 2 cut(s) 751, 1135
MluCI AATT 7 cut(s) 50, 420, 700, 770, 1035, 1201, 1427
MlyI GAGTC 2 cut(s) 59, 83
MmeI TCCRAC 4 cut(s) 96, 378, 636, 673
MroI TCCGGA 2 cut(s) 439, 508
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 6 cut(s) 321, 492, 594, 785, 909, 939
MslI CAYNNNNRTG 1 cut(s) 843
MspI CCGG 6 cut(s) 96, 126, 330, 440, 509, 956
MspR9I CCNGG 3 cut(s) 96, 203, 957
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 3 cut(s) 167, 550, 1416
MwoI GCNNNNNNNGC 6 cut(s) 376, 382, 404, 605, 1415, 1460
NciI CCSGG 2 cut(s) 96, 957
NdeI CATATG 1 cut(s) 400
NdeII GATC 5 cut(s) 142, 505, 739, 835, 1015
NlaIII CATG 4 cut(s) 389, 842, 1346, 1463
NlaIV GGNNCC 4 cut(s) 340, 438, 954, 1353
NmuCI GTSAC 2 cut(s) 185, 722
NspI RCATGY 1 cut(s) 1463
PaeI GCATGC 1 cut(s) 1463
PaeR7I CTCGAG 1 cut(s) 371
PagI TCATGA 1 cut(s) 838
PauI GCGCGC 1 cut(s) 1414
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 279
PctI GAATGC 1 cut(s) 454
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 5 cut(s) 149, 262, 875, 1046, 1058
PflFI GACNNNGTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 955
PleI GAGTC 2 cut(s) 59, 83
PpsI GAGTC 2 cut(s) 59, 83
Psp124BI GAGCTC 2 cut(s) 751, 1135
Psp6I CCWGG 1 cut(s) 201
PspEI GGTNACC 1 cut(s) 185
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 4 cut(s) 340, 438, 954, 1353
PspPI GGNCC 3 cut(s) 268, 635, 1487
PspXI VCTCGAGB 1 cut(s) 371
PstNI CAGNNNCTG 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 107
PteI GCGCGC 1 cut(s) 1414
RseI CAYNNNNRTG 1 cut(s) 843
SacI GAGCTC 2 cut(s) 751, 1135
SaqAI TTAA 6 cut(s) 321, 492, 594, 785, 909, 939
Sau3AI GATC 5 cut(s) 142, 505, 739, 835, 1015
Sau96I GGNCC 3 cut(s) 268, 635, 1487
SchI GAGTC 2 cut(s) 59, 83
ScrFI CCNGG 3 cut(s) 96, 203, 957
SduI GDGCHC 2 cut(s) 751, 1135
SfaNI GCATC 4 cut(s) 394, 520, 532, 1180
SfcI CTRYAG 1 cut(s) 1272
Sfr274I CTCGAG 1 cut(s) 371
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 2 cut(s) 268, 1487
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 843
SmlI CTYRAG 3 cut(s) 371, 893, 1106
SmoI CTYRAG 3 cut(s) 371, 893, 1106
SphI GCATGC 1 cut(s) 1463
Sse9I AATT 7 cut(s) 50, 420, 700, 770, 1035, 1201, 1427
SsiI CCGC 8 cut(s) 224, 227, 333, 342, 383, 550, 1375, 1382
SspMI CTAG 1 cut(s) 1050
SstI GAGCTC 2 cut(s) 751, 1135
StyD4I CCNGG 3 cut(s) 94, 201, 955
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 6 cut(s) 107, 290, 478, 1076, 1084, 1174
TaiI ACGT 4 cut(s) 393, 903, 1405, 1504
TaqI TCGA 1 cut(s) 372
TasI AATT 7 cut(s) 50, 420, 700, 770, 1035, 1201, 1427
TauI GCSGC 3 cut(s) 227, 230, 1385
TfiI GAWTC 5 cut(s) 149, 262, 875, 1046, 1058
Tru1I TTAA 6 cut(s) 321, 492, 594, 785, 909, 939
Tru9I TTAA 6 cut(s) 321, 492, 594, 785, 909, 939
TseFI GTSAC 2 cut(s) 185, 722
TseI GCWGC 8 cut(s) 376, 988, 1159, 1231, 1268, 1324, 1456, 1475
Tsp45I GTSAC 2 cut(s) 185, 722
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 268, 1487
XapI RAATTY 2 cut(s) 420, 770
XceI RCATGY 1 cut(s) 1463
XhoI CTCGAG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 1 cut(s) 1050
ZraI GACGTC 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.