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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 35, 248 |
| AclWI | GGATC | 4 cut(s) | 151, 210, 258, 272 |
| AcsI | RAATTY | 1 cut(s) | 29 |
| AfaI | GTAC | 1 cut(s) | 141 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 41 |
| AgsI | TTSAA | 1 cut(s) | 110 |
| AlwI | GGATC | 4 cut(s) | 151, 210, 258, 272 |
| ApoI | RAATTY | 1 cut(s) | 29 |
| AspLEI | GCGC | 1 cut(s) | 133 |
| AsuC2I | CCSGG | 1 cut(s) | 89 |
| AsuHPI | GGTGA | 1 cut(s) | 302 |
| BceAI | ACGGC | 1 cut(s) | 300 |
| BcnI | CCSGG | 1 cut(s) | 89 |
| BfoI | RGCGCY | 1 cut(s) | 134 |
| Bme1390I | CCNGG | 1 cut(s) | 89 |
| BmrFI | CCNGG | 1 cut(s) | 89 |
| BpuMI | CCSGG | 1 cut(s) | 89 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 41 |
| BseGI | GGATG | 1 cut(s) | 231 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 41 |
| BseMII | CTCAG | 1 cut(s) | 197 |
| BsiSI | CCGG | 1 cut(s) | 88 |
| BslFI | GGGAC | 1 cut(s) | 191 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 41 |
| BsmFI | GGGAC | 1 cut(s) | 191 |
| Bsp143I | GATC | 5 cut(s) | 156, 202, 215, 250, 264 |
| BspACI | CCGC | 2 cut(s) | 35, 248 |
| BspCNI | CTCAG | 1 cut(s) | 198 |
| BspHI | TCATGA | 1 cut(s) | 25 |
| BspPI | GGATC | 4 cut(s) | 151, 210, 258, 272 |
| BssMI | GATC | 5 cut(s) | 156, 202, 215, 250, 264 |
| Bst4CI | ACNGT | 1 cut(s) | 182 |
| BstC8I | GCNNGC | 1 cut(s) | 69 |
| BstDEI | CTNAG | 1 cut(s) | 206 |
| BstF5I | GGATG | 1 cut(s) | 231 |
| BstH2I | RGCGCY | 1 cut(s) | 134 |
| BstHHI | GCGC | 1 cut(s) | 133 |
| BstKTI | GATC | 5 cut(s) | 159, 205, 218, 253, 267 |
| BstMBI | GATC | 5 cut(s) | 156, 202, 215, 250, 264 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 73 |
| BstSCI | CCNGG | 1 cut(s) | 87 |
| BstX2I | RGATCY | 3 cut(s) | 156, 215, 264 |
| BstYI | RGATCY | 3 cut(s) | 156, 215, 264 |
| BtsCI | GGATG | 1 cut(s) | 231 |
| Cac8I | GCNNGC | 1 cut(s) | 69 |
| CciI | TCATGA | 1 cut(s) | 25 |
| CfoI | GCGC | 1 cut(s) | 133 |
| Csp6I | GTAC | 1 cut(s) | 140 |
| CviAII | CATG | 3 cut(s) | 26, 38, 319 |
| CviJI | RGCY | 4 cut(s) | 67, 103, 200, 287 |
| CviKI_1 | RGCY | 4 cut(s) | 67, 103, 200, 287 |
| CviQI | GTAC | 1 cut(s) | 140 |
| DdeI | CTNAG | 1 cut(s) | 206 |
| DpnI | GATC | 5 cut(s) | 158, 204, 217, 252, 266 |
| DpnII | GATC | 5 cut(s) | 156, 202, 215, 250, 264 |
| EciI | GGCGGA | 1 cut(s) | 263 |
| FaeI | CATG | 3 cut(s) | 29, 41, 322 |
| FaiI | YATR | 8 cut(s) | 27, 39, 84, 95, 164, 297, 311, 320 |
| FaqI | GGGAC | 1 cut(s) | 191 |
| FatI | CATG | 3 cut(s) | 25, 37, 318 |
| FokI | GGATG | 1 cut(s) | 238 |
| GlaI | GCGC | 1 cut(s) | 132 |
| HaeII | RGCGCY | 1 cut(s) | 134 |
| HapII | CCGG | 1 cut(s) | 88 |
| HhaI | GCGC | 1 cut(s) | 133 |
| Hin1II | CATG | 3 cut(s) | 29, 41, 322 |
| Hin6I | GCGC | 1 cut(s) | 131 |
| HinP1I | GCGC | 1 cut(s) | 131 |
| HincII | GTYRAC | 1 cut(s) | 316 |
| HindII | GTYRAC | 1 cut(s) | 316 |
| HinfI | GANTC | 2 cut(s) | 97, 229 |
| HpaII | CCGG | 1 cut(s) | 88 |
| HphI | GGTGA | 1 cut(s) | 302 |
| Hpy166II | GTNNAC | 1 cut(s) | 316 |
| Hpy188I | TCNGA | 2 cut(s) | 207, 220 |
| Hpy188III | TCNNGA | 1 cut(s) | 26 |
| Hpy8I | GTNNAC | 1 cut(s) | 316 |
| HpyAV | CCTTC | 2 cut(s) | 35, 104 |
| HpyCH4III | ACNGT | 1 cut(s) | 182 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 73 |
| HpyF3I | CTNAG | 1 cut(s) | 206 |
| Hsp92II | CATG | 3 cut(s) | 29, 41, 322 |
| HspAI | GCGC | 1 cut(s) | 131 |
| Kzo9I | GATC | 5 cut(s) | 156, 202, 215, 250, 264 |
| LpnPI | CCDG | 4 cut(s) | 42, 81, 101, 273 |
| MaeIII | GTNAC | 1 cut(s) | 290 |
| MalI | GATC | 5 cut(s) | 158, 204, 217, 252, 266 |
| MboI | GATC | 5 cut(s) | 156, 202, 215, 250, 264 |
| MboII | GAAGA | 1 cut(s) | 166 |
| MflI | RGATCY | 3 cut(s) | 156, 215, 264 |
| MluCI | AATT | 1 cut(s) | 29 |
| MnlI | CCTC | 3 cut(s) | 201, 269, 320 |
| MspI | CCGG | 1 cut(s) | 88 |
| MspR9I | CCNGG | 1 cut(s) | 89 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 73 |
| NciI | CCSGG | 1 cut(s) | 89 |
| NdeII | GATC | 5 cut(s) | 156, 202, 215, 250, 264 |
| NlaIII | CATG | 3 cut(s) | 29, 41, 322 |
| NmuCI | GTSAC | 1 cut(s) | 290 |
| PagI | TCATGA | 1 cut(s) | 25 |
| PfeI | GAWTC | 2 cut(s) | 97, 229 |
| PsuI | RGATCY | 3 cut(s) | 156, 215, 264 |
| RsaI | GTAC | 1 cut(s) | 141 |
| RsaNI | GTAC | 1 cut(s) | 140 |
| Sau3AI | GATC | 5 cut(s) | 156, 202, 215, 250, 264 |
| ScrFI | CCNGG | 1 cut(s) | 89 |
| SetI | ASST | 5 cut(s) | 61, 188, 212, 280, 331 |
| Sse9I | AATT | 1 cut(s) | 29 |
| SsiI | CCGC | 2 cut(s) | 35, 248 |
| StyD4I | CCNGG | 1 cut(s) | 87 |
| TaaI | ACNGT | 1 cut(s) | 182 |
| TasI | AATT | 1 cut(s) | 29 |
| TfiI | GAWTC | 2 cut(s) | 97, 229 |
| TseFI | GTSAC | 1 cut(s) | 290 |
| Tsp45I | GTSAC | 1 cut(s) | 290 |
| TspDTI | ATGAA | 4 cut(s) | 42, 54, 242, 335 |
| XapI | RAATTY | 1 cut(s) | 29 |