Rroxscaffold_2G00077400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001391
Physical Location & Seq
Forward (+)
939610 .. 940570
961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00077400.1

Sequence Viewer

Length: 462 bp
ATGGGGGAGACCAATAATGGTTATATGCGTTGTGAACTGAAAGTGGATACCAGTTCCAAGGGATGGCATATGACCATGATCAAAGTTTTGAATAGTATCAAAGGAGTGAAATACTACATTGATGCAGAAGCTGGAATCGCATATGTTGCTGGTACGATCCACCCAGACACCCTTCTGAGAGTGTTAGCAAAGTCTGGAAGGCATGCTCAGCTGCTTAGGGTTGATTCAGGATATCGTCAACAAGTCTTGAAGGAGAAAAGTACTAGTACTAGCTATGGTTATGGCCCCAACGTTCAGGGGAAAACTAGTAGTAGCTCTCATGGTTATGGTCCTAACTGTAACAATTATGGGGGGGATTCTTATGGTCAACTTCTCAGTAGGAGCTACGAACAACTAGACAATCAGTCGACTATGGTGAGTCACCGATTTTATGATGCAGACACCCAGTGCACCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.95

Weight (kDa)

8.71

Isoelectric Point (pI)

20.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 63
AccI GTMKAC 1 cut(s) 407
AclI AACGTT 1 cut(s) 291
AclWI GGATC 1 cut(s) 151
AdeI CACNNNGTG 1 cut(s) 447
AfaI GTAC 3 cut(s) 154, 262, 268
AfiI CCNNNNNNNGG 1 cut(s) 63
AgsI TTSAA 2 cut(s) 91, 250
AhdI GACNNNNNGTC 1 cut(s) 403
AhlI ACTAGT 2 cut(s) 263, 305
AluBI AGCT 5 cut(s) 131, 211, 273, 315, 384
AluI AGCT 5 cut(s) 131, 211, 273, 315, 384
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 2
Alw44I GTGCAC 1 cut(s) 448
AlwI GGATC 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 1 cut(s) 283
ApaLI GTGCAC 1 cut(s) 448
ApeKI GCWGC 1 cut(s) 211
AspS9I GGNCC 2 cut(s) 284, 329
AsuHPI GGTGA 2 cut(s) 413, 427
AvaII GGWCC 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 452
BbvI GCAGC 1 cut(s) 198
BccI CCATC 1 cut(s) 57
BciVI GTATCC 1 cut(s) 40
BclI TGATCA 1 cut(s) 78
BcoDI GTCTC 1 cut(s) 2
BcuI ACTAGT 2 cut(s) 263, 305
BfaI CTAG 4 cut(s) 264, 270, 306, 395
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 1 cut(s) 212
BlpI GCTNAGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 213
BmcAI AGTACT 2 cut(s) 262, 268
Bme18I GGWCC 1 cut(s) 329
BmeRI GACNNNNNGTC 1 cut(s) 403
BmgT120I GGNCC 2 cut(s) 284, 329
BmiI GGNNCC 1 cut(s) 286
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 2 cut(s) 112, 424
BmuI ACTGGG 1 cut(s) 439
Bpu10I CCTNAGC 1 cut(s) 215
Bpu1102I GCTNAGC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 1 cut(s) 57
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse1I ACTGG 2 cut(s) 51, 445
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMII CTCAG 3 cut(s) 167, 221, 388
BseNI ACTGG 2 cut(s) 51, 445
BseSI GKGCMC 1 cut(s) 452
BseXI GCAGC 1 cut(s) 198
BshFI GGCC 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 63
BsmAI GTCTC 1 cut(s) 2
BsnI GGCC 1 cut(s) 285
Bso31I GGTCTC 1 cut(s) 2
Bsp1286I GDGCHC 1 cut(s) 452
Bsp143I GATC 2 cut(s) 78, 156
Bsp1720I GCTNAGC 1 cut(s) 207
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 3 cut(s) 168, 220, 387
BspLI GGNNCC 1 cut(s) 286
BspPI GGATC 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 2
BsrI ACTGG 2 cut(s) 51, 445
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 2 cut(s) 78, 156
BssT1I CCWWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 4 cut(s) 176, 207, 215, 374
BstF5I GGATG 1 cut(s) 68
BstKTI GATC 2 cut(s) 81, 159
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 2 cut(s) 78, 156
BstMWI GCNNNNNNNGC 3 cut(s) 137, 146, 208
BstNSI RCATGY 1 cut(s) 206
BstSLI GKGCMC 1 cut(s) 452
BstV1I GCAGC 1 cut(s) 198
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 1 cut(s) 285
BtsCI GGATG 1 cut(s) 68
BtsIMutI CAGTG 1 cut(s) 452
Cac8I GCNNGC 1 cut(s) 204
CaiI CAGNNNCTG 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 284, 329
Csp6I GTAC 3 cut(s) 153, 261, 267
CviAII CATG 3 cut(s) 76, 203, 320
CviJI RGCY 6 cut(s) 131, 211, 273, 285, 315, 384
CviKI_1 RGCY 6 cut(s) 131, 211, 273, 285, 315, 384
CviQI GTAC 3 cut(s) 153, 261, 267
DdeI CTNAG 4 cut(s) 176, 207, 215, 374
DpnI GATC 2 cut(s) 80, 158
DpnII GATC 2 cut(s) 78, 156
DraIII CACNNNGTG 1 cut(s) 447
DriI GACNNNNNGTC 1 cut(s) 403
Eam1105I GACNNNNNGTC 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 2
Eco32I GATATC 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 329
EcoRV GATATC 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 3 cut(s) 79, 206, 323
FatI CATG 3 cut(s) 75, 202, 319
FauNDI CATATG 2 cut(s) 69, 142
FbaI TGATCA 1 cut(s) 78
FblI GTMKAC 1 cut(s) 407
Fnu4HI GCNGC 1 cut(s) 212
FokI GGATG 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 212
FspBI CTAG 4 cut(s) 264, 270, 306, 395
GluI GCNGC 1 cut(s) 212
HaeIII GGCC 1 cut(s) 285
Hin1II CATG 3 cut(s) 79, 206, 323
HincII GTYRAC 3 cut(s) 239, 368, 408
HindII GTYRAC 3 cut(s) 239, 368, 408
HinfI GANTC 4 cut(s) 135, 224, 356, 418
HphI GGTGA 2 cut(s) 413, 427
Hpy166II GTNNAC 5 cut(s) 35, 239, 368, 408, 450
Hpy188I TCNGA 1 cut(s) 177
Hpy188III TCNNGA 3 cut(s) 195, 228, 247
Hpy8I GTNNAC 5 cut(s) 35, 239, 368, 408, 450
HpyAV CCTTC 3 cut(s) 182, 192, 244
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 3 cut(s) 125, 437, 450
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 146, 208
HpyF3I CTNAG 4 cut(s) 176, 207, 215, 374
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 3 cut(s) 79, 206, 323
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 2 cut(s) 78, 156
LmnI GCTCC 1 cut(s) 381
LpnPI CCDG 8 cut(s) 64, 117, 135, 177, 180, 213, 281, 458
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 2 cut(s) 112, 424
MaeI CTAG 4 cut(s) 264, 270, 306, 395
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 338, 419
MalI GATC 2 cut(s) 80, 158
MboI GATC 2 cut(s) 78, 156
MhlI GDGCHC 1 cut(s) 452
MluCI AATT 1 cut(s) 343
MlyI GAGTC 1 cut(s) 427
MslI CAYNNNNRTG 2 cut(s) 324, 455
MspA1I CMGCKG 1 cut(s) 211
MwoI GCNNNNNNNGC 3 cut(s) 137, 146, 208
NdeI CATATG 2 cut(s) 69, 142
NdeII GATC 2 cut(s) 78, 156
NlaIII CATG 3 cut(s) 79, 206, 323
NlaIV GGNNCC 1 cut(s) 286
NmuCI GTSAC 1 cut(s) 419
NspI RCATGY 1 cut(s) 206
PaeI GCATGC 1 cut(s) 206
PfeI GAWTC 3 cut(s) 135, 224, 356
PflMI CCANNNNNTGG 1 cut(s) 63
PkrI GCNGC 1 cut(s) 213
PleI GAGTC 1 cut(s) 426
PpsI GAGTC 1 cut(s) 426
Psp1406I AACGTT 1 cut(s) 291
PspN4I GGNNCC 1 cut(s) 286
PspPI GGNCC 2 cut(s) 284, 329
PstNI CAGNNNCTG 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 211
RsaI GTAC 3 cut(s) 154, 262, 268
RsaNI GTAC 3 cut(s) 153, 261, 267
RseI CAYNNNNRTG 2 cut(s) 324, 455
SalI GTCGAC 1 cut(s) 406
SatI GCNGC 1 cut(s) 212
Sau3AI GATC 2 cut(s) 78, 156
Sau96I GGNCC 2 cut(s) 284, 329
ScaI AGTACT 2 cut(s) 262, 268
SchI GAGTC 1 cut(s) 427
SduI GDGCHC 1 cut(s) 452
SetI ASST 6 cut(s) 133, 213, 275, 294, 317, 386
SfaNI GCATC 2 cut(s) 112, 424
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 324, 455
SpeI ACTAGT 2 cut(s) 263, 305
SphI GCATGC 1 cut(s) 206
Sse9I AATT 1 cut(s) 343
SspMI CTAG 4 cut(s) 264, 270, 306, 395
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 338
TaiI ACGT 1 cut(s) 294
TaqI TCGA 1 cut(s) 407
TasI AATT 1 cut(s) 343
TatI WGTACW 2 cut(s) 260, 266
TfiI GAWTC 3 cut(s) 135, 224, 356
TscAI CASTG 1 cut(s) 452
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 1 cut(s) 211
Tsp45I GTSAC 1 cut(s) 419
TspRI CASTG 1 cut(s) 452
Van91I CCANNNNNTGG 1 cut(s) 63
VneI GTGCAC 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 329
XceI RCATGY 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 407
XspI CTAG 4 cut(s) 264, 270, 306, 395
ZrmI AGTACT 2 cut(s) 262, 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.