FvH4_1g00760

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002120
Physical Location & Seq
Reverse (-)
385795 .. 387959
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00760.t1

Sequence Viewer

Length: 930 bp
ATGGACCAAACCAGAAACGAAGACACCGACGAGTCACCGCCGGAGTCCGAGTCCGGCTGGGCCGAGTTGACCCACGAGTGTCTCAGCAACATCATCGCCCGCCTCTCCTTGGAGCACCGATGGCGTGCGATGCTCGTCTGCAAGTCATGGCTGCTCGCCTGCAAGGACCCGCATCTCAACTCTGGCTTCGTCCTCGAGCCCGAGTCAGTGCCCGAGTCGGCTCGGTGGTGGGCCCCCGATTTAGGGAGGAAGGTCGATTCCATTCTCCGATCTGTTGCTGAATGGAGCCAGGGGTCGCTTAGGGAGATTCGCACCCGGAACTGCTCTAATGCGTCTCTCTCTTTCGTCGCCCAAAGGTGCCCAAACCTTGAGGTTCTTTCGATCAAGAGCTGCCCGAATGTGACTGATGCGTCTATGGCTGATATAGCGTTTCGGTGTCCCATGATTAAGGAACTTGACATCAGCTATTGCCATGAAATATCTCACGCGGCTCTGCTGTTGATTGGGAGGAATTGCCCTAATCTCACAATTGTTAAACGGAATGCGTTGAATTTGCTAGATCCCTCTGAACATGCTGGGATTGTTCCTGATGAGTACTTAAAAACTTGCCCTCAAGATGGCAATTCAGAAGCTAATGCAATTGGCAAATCTATGCCGAATCTGAAGCACCTTGAACTGAGGTTCTCCAAGGTAACGGCTAAAGGCCTTGGTTTGATATCTGCAGGGTGTCTGAAACTGGAGTACTTGGATGTGTTTGGGTGTACAAACTTGACTAATCGTGATATCAACAATGCAACATCCGAGCTGAAGAACTTGAAGGAGATAAAAAAGCCAAATTTCTACATCCCTAGGTCGGTTTTCCATACGGAGAGGTATGGACATTGGAGGCTGTATGATGAGAGATTTCAAACAGATGTTTTCAGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

310

Amino Acids

34.99

Weight (kDa)

6.32

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 59 2.9e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 357
AccII CGCG 1 cut(s) 488
AciI CCGC 4 cut(s) 38, 100, 170, 488
AclWI GGATC 1 cut(s) 554
AcsI RAATTY 2 cut(s) 550, 835
AcuI CTGAAG 2 cut(s) 683, 827
AfaI GTAC 3 cut(s) 596, 743, 763
AfiI CCNNNNNNNGG 5 cut(s) 109, 242, 243, 617, 853
AgsI TTSAA 4 cut(s) 550, 674, 817, 908
AjnI CCWGG 1 cut(s) 288
AluBI AGCT 4 cut(s) 390, 465, 632, 805
AluI AGCT 4 cut(s) 390, 465, 632, 805
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 86, 339
AlwI GGATC 1 cut(s) 554
AlwNI CAGNNNCTG 1 cut(s) 927
Ama87I CYCGRG 3 cut(s) 194, 200, 212
AoxI GGCC 3 cut(s) 60, 231, 703
ApaI GGGCCC 1 cut(s) 235
ApeKI GCWGC 2 cut(s) 151, 390
ApoI RAATTY 2 cut(s) 550, 835
AspA2I CCTAGG 1 cut(s) 848
AspS9I GGNCC 5 cut(s) 4, 60, 166, 231, 232
AsuC2I CCSGG 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 27
AvaI CYCGRG 3 cut(s) 194, 200, 212
AvaII GGWCC 2 cut(s) 4, 166
AvrII CCTAGG 1 cut(s) 848
BaeGI GKGCMC 3 cut(s) 213, 235, 362
BanI GGYRCC 1 cut(s) 357
BanII GRGCYC 2 cut(s) 201, 235
BauI CACGAG 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 138, 377
BccI CCATC 2 cut(s) 114, 611
BceAI ACGGC 1 cut(s) 711
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 290
BcnI CCSGG 1 cut(s) 316
BcoDI GTCTC 2 cut(s) 86, 339
BfaI CTAG 2 cut(s) 557, 849
BfmI CTRYAG 1 cut(s) 720
BisI GCNGC 3 cut(s) 152, 391, 489
BlnI CCTAGG 1 cut(s) 848
BlsI GCNGC 3 cut(s) 153, 392, 490
BmcAI AGTACT 2 cut(s) 596, 743
Bme1390I CCNGG 2 cut(s) 290, 316
Bme18I GGWCC 2 cut(s) 4, 166
BmeT110I CYCGRG 3 cut(s) 194, 200, 212
BmgT120I GGNCC 5 cut(s) 4, 60, 166, 231, 232
BmiI GGNNCC 5 cut(s) 168, 233, 234, 287, 359
BmrFI CCNGG 2 cut(s) 290, 316
BmsI GCATC 3 cut(s) 120, 181, 397
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 758
Bpu10I CCTNAGC 1 cut(s) 299
BpuEI CTTGAG 2 cut(s) 389, 597
BpuMI CCSGG 1 cut(s) 316
BsaJI CCNNGG 5 cut(s) 108, 289, 687, 706, 848
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 5 cut(s) 109, 242, 243, 617, 853
Bse1I ACTGG 1 cut(s) 741
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 5 cut(s) 108, 289, 687, 706, 848
BseGI GGATG 3 cut(s) 754, 797, 843
BseLI CCNNNNNNNGG 5 cut(s) 109, 242, 243, 617, 853
BseMII CTCAG 2 cut(s) 97, 668
BseNI ACTGG 1 cut(s) 741
BseSI GKGCMC 3 cut(s) 213, 235, 362
BseXI GCAGC 2 cut(s) 138, 377
BseYI CCCAGC 2 cut(s) 57, 575
Bsh1236I CGCG 1 cut(s) 488
BshFI GGCC 3 cut(s) 62, 233, 705
BshNI GGYRCC 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 3 cut(s) 194, 200, 212
BsiSI CCGG 3 cut(s) 41, 54, 316
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 5 cut(s) 109, 242, 243, 617, 853
BsmAI GTCTC 2 cut(s) 86, 339
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 423
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 3 cut(s) 62, 233, 705
BsoBI CYCGRG 3 cut(s) 194, 200, 212
Bsp120I GGGCCC 1 cut(s) 231
Bsp1286I GDGCHC 5 cut(s) 117, 201, 213, 235, 362
Bsp1407I TGTACA 1 cut(s) 761
Bsp143I GATC 3 cut(s) 269, 381, 559
BspACI CCGC 4 cut(s) 38, 100, 170, 488
BspANI GGCC 3 cut(s) 62, 233, 705
BspCNI CTCAG 2 cut(s) 96, 669
BspFNI CGCG 1 cut(s) 488
BspLI GGNNCC 5 cut(s) 168, 233, 234, 287, 359
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 357
BsrGI TGTACA 1 cut(s) 761
BsrI ACTGG 1 cut(s) 741
BssECI CCNNGG 5 cut(s) 108, 289, 687, 706, 848
BssMI GATC 3 cut(s) 269, 381, 559
BssSI CACGAG 1 cut(s) 74
BssT1I CCWWGG 4 cut(s) 108, 687, 706, 848
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 1 cut(s) 290
BstAUI TGTACA 1 cut(s) 761
BstC8I GCNNGC 4 cut(s) 100, 126, 156, 160
BstDEI CTNAG 3 cut(s) 83, 299, 677
BstF5I GGATG 3 cut(s) 754, 797, 843
BstFNI CGCG 1 cut(s) 488
BstKTI GATC 3 cut(s) 272, 384, 562
BstMAI GTCTC 2 cut(s) 86, 339
BstMBI GATC 3 cut(s) 269, 381, 559
BstMWI GCNNNNNNNGC 4 cut(s) 121, 130, 416, 425
BstNI CCWGG 1 cut(s) 290
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 2 cut(s) 288, 314
BstSFI CTRYAG 1 cut(s) 720
BstSLI GKGCMC 3 cut(s) 213, 235, 362
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 2 cut(s) 138, 377
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 3 cut(s) 62, 233, 705
BtgZI GCGATG 2 cut(s) 79, 143
BtsCI GGATG 3 cut(s) 754, 797, 843
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 4 cut(s) 100, 126, 156, 160
CaiI CAGNNNCTG 1 cut(s) 927
Cfr13I GGNCC 5 cut(s) 4, 60, 166, 231, 232
CseI GACGC 2 cut(s) 321, 399
Csp6I GTAC 3 cut(s) 595, 742, 762
CviAII CATG 4 cut(s) 147, 442, 473, 572
CviQI GTAC 3 cut(s) 595, 742, 762
DdeI CTNAG 3 cut(s) 83, 299, 677
DpnI GATC 3 cut(s) 271, 383, 561
DpnII GATC 3 cut(s) 269, 381, 559
DrdI GACNNNNNNGTC 1 cut(s) 409
DseDI GACNNNNNNGTC 1 cut(s) 409
Eco130I CCWWGG 4 cut(s) 108, 687, 706, 848
Eco147I AGGCCT 1 cut(s) 705
Eco24I GRGCYC 2 cut(s) 201, 235
Eco32I GATATC 2 cut(s) 717, 784
Eco47I GGWCC 2 cut(s) 4, 166
Eco57I CTGAAG 2 cut(s) 683, 827
Eco88I CYCGRG 3 cut(s) 194, 200, 212
EcoO109I RGGNCCY 2 cut(s) 166, 232
EcoRII CCWGG 1 cut(s) 288
EcoRV GATATC 2 cut(s) 717, 784
EcoT14I CCWWGG 4 cut(s) 108, 687, 706, 848
EcoT38I GRGCYC 2 cut(s) 201, 235
ErhI CCWWGG 4 cut(s) 108, 687, 706, 848
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 4 cut(s) 150, 445, 476, 575
FaqI GGGAC 1 cut(s) 423
FatI CATG 4 cut(s) 146, 441, 472, 571
FauI CCCGC 2 cut(s) 107, 177
Fnu4HI GCNGC 3 cut(s) 152, 391, 489
FokI GGATG 3 cut(s) 761, 784, 830
FriOI GRGCYC 2 cut(s) 201, 235
Fsp4HI GCNGC 3 cut(s) 152, 391, 489
FspBI CTAG 2 cut(s) 557, 849
GluI GCNGC 3 cut(s) 152, 391, 489
GsaI CCCAGC 2 cut(s) 61, 579
GsuI CTGGAG 1 cut(s) 758
HaeIII GGCC 3 cut(s) 62, 233, 705
HapII CCGG 3 cut(s) 41, 54, 316
HgaI GACGC 2 cut(s) 321, 399
Hin1II CATG 4 cut(s) 150, 445, 476, 575
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 9 cut(s) 32, 44, 50, 203, 215, 257, 307, 658, 924
HpaII CCGG 3 cut(s) 41, 54, 316
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 2 cut(s) 69, 762
Hpy188I TCNGA 9 cut(s) 49, 269, 568, 628, 663, 732, 802, 923, 929
Hpy188III TCNNGA 4 cut(s) 385, 587, 614, 779
Hpy8I GTNNAC 2 cut(s) 69, 762
Hpy99I CGWCG 2 cut(s) 32, 350
HpyAV CCTTC 2 cut(s) 244, 811
HpyCH4V TGCA 5 cut(s) 141, 162, 638, 722, 794
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 130, 416, 425
HpyF3I CTNAG 3 cut(s) 83, 299, 677
Hsp92II CATG 4 cut(s) 150, 445, 476, 575
Kzo9I GATC 3 cut(s) 269, 381, 559
LmnI GCTCC 2 cut(s) 112, 285
Lsp1109I GCAGC 2 cut(s) 138, 377
LweI GCATC 3 cut(s) 120, 181, 397
MaeI CTAG 2 cut(s) 557, 849
MaeIII GTNAC 3 cut(s) 33, 400, 691
MalI GATC 3 cut(s) 271, 383, 561
MboI GATC 3 cut(s) 269, 381, 559
MboII GAAGA 2 cut(s) 32, 820
MfeI CAATTG 2 cut(s) 528, 639
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 5 cut(s) 117, 201, 213, 235, 362
MluCI AATT 6 cut(s) 511, 528, 550, 622, 639, 835
MlyI GAGTC 5 cut(s) 41, 53, 59, 212, 224
MseI TTAA 3 cut(s) 447, 534, 599
MspI CCGG 3 cut(s) 41, 54, 316
MspR9I CCNGG 2 cut(s) 290, 316
MunI CAATTG 2 cut(s) 528, 639
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 1 cut(s) 290
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 4 cut(s) 121, 130, 416, 425
NciI CCSGG 1 cut(s) 316
NdeII GATC 3 cut(s) 269, 381, 559
NlaIII CATG 4 cut(s) 150, 445, 476, 575
NlaIV GGNNCC 5 cut(s) 168, 233, 234, 287, 359
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 33, 400
NspI RCATGY 1 cut(s) 575
PaeR7I CTCGAG 1 cut(s) 194
PceI AGGCCT 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 1 cut(s) 547
PfeI GAWTC 4 cut(s) 257, 307, 658, 924
PkrI GCNGC 3 cut(s) 153, 392, 490
PleI GAGTC 5 cut(s) 40, 52, 58, 211, 223
PpsI GAGTC 5 cut(s) 40, 52, 58, 211, 223
PpuMI RGGWCCY 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 288
PspFI CCCAGC 2 cut(s) 57, 575
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 5 cut(s) 168, 233, 234, 287, 359
PspOMI GGGCCC 1 cut(s) 231
PspPI GGNCC 5 cut(s) 4, 60, 166, 231, 232
PspPPI RGGWCCY 1 cut(s) 166
PspXI VCTCGAGB 1 cut(s) 194
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 927
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 3 cut(s) 596, 743, 763
RsaNI GTAC 3 cut(s) 595, 742, 762
SaqAI TTAA 3 cut(s) 447, 534, 599
SatI GCNGC 3 cut(s) 152, 391, 489
Sau3AI GATC 3 cut(s) 269, 381, 559
Sau96I GGNCC 5 cut(s) 4, 60, 166, 231, 232
ScaI AGTACT 2 cut(s) 596, 743
SchI GAGTC 5 cut(s) 41, 53, 59, 212, 224
ScrFI CCNGG 2 cut(s) 290, 316
SduI GDGCHC 5 cut(s) 117, 201, 213, 235, 362
SfaNI GCATC 3 cut(s) 120, 181, 397
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 2 cut(s) 4, 166
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 3 cut(s) 194, 368, 612
SmoI CTYRAG 3 cut(s) 194, 368, 612
Sse9I AATT 6 cut(s) 511, 528, 550, 622, 639, 835
SseBI AGGCCT 1 cut(s) 705
SsiI CCGC 4 cut(s) 38, 100, 170, 488
SspMI CTAG 2 cut(s) 557, 849
StuI AGGCCT 1 cut(s) 705
StyD4I CCNGG 2 cut(s) 288, 314
StyI CCWWGG 4 cut(s) 108, 687, 706, 848
TaqI TCGA 3 cut(s) 195, 255, 380
TasI AATT 6 cut(s) 511, 528, 550, 622, 639, 835
TatI WGTACW 3 cut(s) 594, 741, 761
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 4 cut(s) 257, 307, 658, 924
Tru1I TTAA 3 cut(s) 447, 534, 599
Tru9I TTAA 3 cut(s) 447, 534, 599
TscAI CASTG 1 cut(s) 213
TseFI GTSAC 2 cut(s) 33, 400
TseI GCWGC 2 cut(s) 151, 390
Tsp45I GTSAC 2 cut(s) 33, 400
TspDTI ATGAA 1 cut(s) 489
TspGWI ACGGA 2 cut(s) 553, 881
TspRI CASTG 1 cut(s) 213
VpaK11BI GGWCC 2 cut(s) 4, 166
XapI RAATTY 2 cut(s) 550, 835
XceI RCATGY 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 194
XmaJI CCTAGG 1 cut(s) 848
XspI CTAG 2 cut(s) 557, 849
ZrmI AGTACT 2 cut(s) 596, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.