Rw2G000860

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002120
Physical Location & Seq
Reverse (-)
727174 .. 728528
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000860.1

Sequence Viewer

Length: 939 bp
ATGGACCGAACCAATAATGAAGAAGAAGAAGAAGAAGAACCACCAGTCTGCGACTGGGCCGGGTTGACCTACGTGTGCCTCGCCAACATCCTCGCCCGGCTTCCCTTGGAGCACCGATGGCGTGCGATGCTCCTCTGCAAATCATGGCTGCTCGCCTGCAAGGACCCGCATCTCAACTCGCGCTTCGTCCTCGAGCCCGAGCCCAAGCCCAAGCGCGAGTACGGGTCGGCTCAGTGGTGGGGCCCCGAGTTGGGGAGAAGGGTCGATTCCATGCTCCGATCTGTTGCTGAATGGAGCGAGGGGTCGCTTAGGGAGATTCGCACCCGGAACTGCTCTAATGAATCTCTCCATTTCGTCGCCCAAAGGTGCCCAAACCTTGAGGTTCTTTCGATCAAGTGCTGCCCGAATGTGACTGATGCATCCATGGCTGACATAGCGTTTCGGTGCATCATGATTAAGGAACTTGACATCAGCTATTGCCATGAAATATCTCACGAGTCTCTGGTGTTGATTGGGAAGAATTGCCCTAATCTCACAGTTGTTAAACGGAATGCGTTGAATTTGCTGGATCCTTCTGAACACGCTGGGATTATTCCTGAGGAGTATTTAAAAACTTGCCCTCAAGATGGAAATTCAGAAGCTGATGCAATTGGCAAATCTATGCCGAATCTCAAACACCTTGAACTAAGGTTCTCCAAGGTAACAGCTAAAGGCCTTGGTTTGATATCTGCAGGGTGTCTGAAACTTGAGTACTTGGATGTGTTTGGGTGTGTAAACTTGAATAGTCGTGATATCAACAATGCAACATCTGAGCTGAAGAACTTGAAGGAGATTAAAAAGCCAAATTTCTACATCCCTAGGTCGGTTTTCCATTCGGAGAGGTATGGACATTGGAGGCTGTATGATGAGAGATTTCAAACAGATATTTTCAGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

312

Amino Acids

35.79

Weight (kDa)

6.18

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 21 - 59 1e-05 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 366
AccII CGCG 2 cut(s) 181, 216
AciI CCGC 1 cut(s) 167
AclWI GGATC 2 cut(s) 563, 576
AcsI RAATTY 3 cut(s) 559, 631, 844
AcuI CTGAAG 1 cut(s) 836
AfaI GTAC 2 cut(s) 221, 752
AfiI CCNNNNNNNGG 5 cut(s) 250, 251, 252, 626, 862
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 5 cut(s) 559, 683, 781, 826, 917
AluBI AGCT 4 cut(s) 474, 641, 707, 814
AluI AGCT 4 cut(s) 474, 641, 707, 814
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 2 cut(s) 563, 576
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 3 cut(s) 191, 197, 245
AoxI GGCC 3 cut(s) 57, 241, 712
ApaI GGGCCC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 148, 399
ApoI RAATTY 3 cut(s) 559, 631, 844
AspA2I CCTAGG 1 cut(s) 857
AspLEI GCGC 2 cut(s) 183, 216
AspS9I GGNCC 5 cut(s) 4, 57, 163, 241, 242
AsuC2I CCSGG 3 cut(s) 61, 97, 325
AvaI CYCGRG 3 cut(s) 191, 197, 245
AvaII GGWCC 2 cut(s) 4, 163
AvrII CCTAGG 1 cut(s) 857
AxyI CCTNAGG 1 cut(s) 597
BaeGI GKGCMC 2 cut(s) 245, 371
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 366
BanII GRGCYC 3 cut(s) 198, 204, 245
BauI CACGAG 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 135, 386
BccI CCATC 2 cut(s) 111, 620
BcnI CCSGG 3 cut(s) 61, 97, 325
BcoDI GTCTC 1 cut(s) 504
BfaI CTAG 1 cut(s) 858
BfmI CTRYAG 1 cut(s) 729
BisI GCNGC 2 cut(s) 149, 400
BlnI CCTAGG 1 cut(s) 857
BlsI GCNGC 2 cut(s) 150, 401
BmcAI AGTACT 1 cut(s) 752
Bme1390I CCNGG 3 cut(s) 61, 97, 325
Bme18I GGWCC 2 cut(s) 4, 163
BmeT110I CYCGRG 3 cut(s) 191, 197, 245
BmgT120I GGNCC 5 cut(s) 4, 57, 163, 241, 242
BmiI GGNNCC 6 cut(s) 165, 242, 243, 244, 368, 570
BmrFI CCNGG 3 cut(s) 61, 97, 325
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 6 cut(s) 117, 178, 406, 428, 456, 634
BmuI ACTGGG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 308
BpuEI CTTGAG 3 cut(s) 398, 606, 767
BpuMI CCSGG 3 cut(s) 61, 97, 325
BsaAI YACGTR 1 cut(s) 73
BsaJI CCNNGG 5 cut(s) 105, 423, 696, 715, 857
Bsc4I CCNNNNNNNGG 5 cut(s) 250, 251, 252, 626, 862
Bse1I ACTGG 2 cut(s) 44, 59
Bse21I CCTNAGG 1 cut(s) 597
BseDI CCNNGG 5 cut(s) 105, 423, 696, 715, 857
BseGI GGATG 4 cut(s) 87, 419, 763, 852
BseLI CCNNNNNNNGG 5 cut(s) 250, 251, 252, 626, 862
BseMII CTCAG 3 cut(s) 245, 588, 801
BseNI ACTGG 2 cut(s) 44, 59
BseRI GAGGAG 2 cut(s) 122, 614
BseSI GKGCMC 2 cut(s) 245, 371
BseXI GCAGC 2 cut(s) 135, 386
BseYI CCCAGC 1 cut(s) 584
Bsh1236I CGCG 2 cut(s) 181, 216
BshFI GGCC 3 cut(s) 59, 243, 714
BshNI GGYRCC 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 3 cut(s) 191, 197, 245
BsiSI CCGG 3 cut(s) 60, 97, 325
BslI CCNNNNNNNGG 5 cut(s) 250, 251, 252, 626, 862
BsmAI GTCTC 1 cut(s) 504
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 3 cut(s) 59, 243, 714
BsoBI CYCGRG 3 cut(s) 191, 197, 245
Bsp120I GGGCCC 1 cut(s) 241
Bsp1286I GDGCHC 5 cut(s) 114, 198, 204, 245, 371
Bsp143I GATC 3 cut(s) 278, 390, 568
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 1 cut(s) 167
BspANI GGCC 3 cut(s) 59, 243, 714
BspCNI CTCAG 3 cut(s) 244, 589, 802
BspFNI CGCG 2 cut(s) 181, 216
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 6 cut(s) 165, 242, 243, 244, 368, 570
BspMAI CTGCAG 1 cut(s) 733
BspPI GGATC 2 cut(s) 563, 576
BspT107I GGYRCC 1 cut(s) 366
BsrI ACTGG 2 cut(s) 44, 59
BssECI CCNNGG 5 cut(s) 105, 423, 696, 715, 857
BssMI GATC 3 cut(s) 278, 390, 568
BssSI CACGAG 1 cut(s) 494
BssT1I CCWWGG 5 cut(s) 105, 423, 696, 715, 857
Bst2BI CACGAG 1 cut(s) 494
Bst4CI ACNGT 1 cut(s) 538
BstBAI YACGTR 1 cut(s) 73
BstC8I GCNNGC 3 cut(s) 123, 153, 157
BstDEI CTNAG 5 cut(s) 231, 308, 597, 686, 810
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 4 cut(s) 87, 419, 763, 852
BstFNI CGCG 2 cut(s) 181, 216
BstHHI GCGC 2 cut(s) 183, 216
BstKTI GATC 3 cut(s) 281, 393, 571
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 3 cut(s) 278, 390, 568
BstMWI GCNNNNNNNGC 4 cut(s) 118, 127, 425, 434
BstSCI CCNGG 3 cut(s) 59, 95, 323
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 2 cut(s) 245, 371
BstUI CGCG 2 cut(s) 181, 216
BstV1I GCAGC 2 cut(s) 135, 386
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 3 cut(s) 59, 243, 714
BtgI CCRYGG 1 cut(s) 423
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 4 cut(s) 87, 419, 763, 852
BtsIMutI CAGTG 1 cut(s) 239
Cac8I GCNNGC 3 cut(s) 123, 153, 157
CaiI CAGNNNCTG 1 cut(s) 641
CciI TCATGA 1 cut(s) 450
CfoI GCGC 2 cut(s) 183, 216
Cfr13I GGNCC 5 cut(s) 4, 57, 163, 241, 242
Csp6I GTAC 2 cut(s) 220, 751
CviAII CATG 5 cut(s) 144, 271, 424, 451, 482
CviQI GTAC 2 cut(s) 220, 751
DdeI CTNAG 5 cut(s) 231, 308, 597, 686, 810
DpnI GATC 3 cut(s) 280, 392, 570
DpnII GATC 3 cut(s) 278, 390, 568
DraI TTTAAA 1 cut(s) 609
Eco130I CCWWGG 5 cut(s) 105, 423, 696, 715, 857
Eco147I AGGCCT 1 cut(s) 714
Eco24I GRGCYC 3 cut(s) 198, 204, 245
Eco32I GATATC 2 cut(s) 726, 793
Eco47I GGWCC 2 cut(s) 4, 163
Eco57I CTGAAG 1 cut(s) 836
Eco81I CCTNAGG 1 cut(s) 597
Eco88I CYCGRG 3 cut(s) 191, 197, 245
EcoO109I RGGNCCY 3 cut(s) 163, 241, 242
EcoRV GATATC 2 cut(s) 726, 793
EcoT14I CCWWGG 5 cut(s) 105, 423, 696, 715, 857
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 3 cut(s) 198, 204, 245
ErhI CCWWGG 5 cut(s) 105, 423, 696, 715, 857
FaeI CATG 5 cut(s) 147, 274, 427, 454, 485
FaiI YATR 9 cut(s) 145, 272, 425, 434, 452, 483, 662, 885, 903
FatI CATG 5 cut(s) 143, 270, 423, 450, 481
FauI CCCGC 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 149, 400
FokI GGATG 4 cut(s) 74, 406, 770, 839
FriOI GRGCYC 3 cut(s) 198, 204, 245
Fsp4HI GCNGC 2 cut(s) 149, 400
FspBI CTAG 1 cut(s) 858
GlaI GCGC 2 cut(s) 182, 215
GluI GCNGC 2 cut(s) 149, 400
GsaI CCCAGC 1 cut(s) 588
HaeIII GGCC 3 cut(s) 59, 243, 714
HapII CCGG 3 cut(s) 60, 97, 325
HhaI GCGC 2 cut(s) 183, 216
Hin1II CATG 5 cut(s) 147, 274, 427, 454, 485
Hin6I GCGC 2 cut(s) 181, 214
HinP1I GCGC 2 cut(s) 181, 214
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HinfI GANTC 6 cut(s) 266, 316, 341, 497, 667, 933
HpaII CCGG 3 cut(s) 60, 97, 325
Hpy166II GTNNAC 2 cut(s) 66, 775
Hpy188I TCNGA 7 cut(s) 278, 577, 637, 741, 811, 877, 932
Hpy188III TCNNGA 5 cut(s) 451, 494, 596, 623, 788
Hpy8I GTNNAC 2 cut(s) 66, 775
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 3 cut(s) 252, 582, 820
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 7 cut(s) 138, 159, 419, 447, 647, 731, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 127, 425, 434
HpyF3I CTNAG 5 cut(s) 231, 308, 597, 686, 810
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 5 cut(s) 147, 274, 427, 454, 485
HspAI GCGC 2 cut(s) 181, 214
Kzo9I GATC 3 cut(s) 278, 390, 568
LmnI GCTCC 4 cut(s) 109, 135, 279, 294
Lsp1109I GCAGC 2 cut(s) 135, 386
LweI GCATC 6 cut(s) 117, 178, 406, 428, 456, 634
MaeI CTAG 1 cut(s) 858
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 2 cut(s) 409, 700
MalI GATC 3 cut(s) 280, 392, 570
MboI GATC 3 cut(s) 278, 390, 568
MboII GAAGA 8 cut(s) 32, 35, 38, 41, 44, 47, 529, 829
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 5 cut(s) 114, 198, 204, 245, 371
MluCI AATT 5 cut(s) 520, 559, 631, 648, 844
MlyI GAGTC 1 cut(s) 506
Mph1103I ATGCAT 1 cut(s) 421
MseI TTAA 4 cut(s) 456, 543, 608, 834
MspI CCGG 3 cut(s) 60, 97, 325
MspR9I CCNGG 3 cut(s) 61, 97, 325
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 556
MvnI CGCG 2 cut(s) 181, 216
MwoI GCNNNNNNNGC 4 cut(s) 118, 127, 425, 434
NciI CCSGG 3 cut(s) 61, 97, 325
NcoI CCATGG 1 cut(s) 423
NdeII GATC 3 cut(s) 278, 390, 568
NlaIII CATG 5 cut(s) 147, 274, 427, 454, 485
NlaIV GGNNCC 6 cut(s) 165, 242, 243, 244, 368, 570
NmuCI GTSAC 1 cut(s) 409
NsiI ATGCAT 1 cut(s) 421
PaeR7I CTCGAG 1 cut(s) 191
PagI TCATGA 1 cut(s) 450
PceI AGGCCT 1 cut(s) 714
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 5 cut(s) 266, 316, 341, 667, 933
PkrI GCNGC 2 cut(s) 150, 401
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
Ppu21I YACGTR 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 163
Psp5II RGGWCCY 1 cut(s) 163
PspFI CCCAGC 1 cut(s) 584
PspN4I GGNNCC 6 cut(s) 165, 242, 243, 244, 368, 570
PspOMI GGGCCC 1 cut(s) 241
PspPI GGNCC 5 cut(s) 4, 57, 163, 241, 242
PspPPI RGGWCCY 1 cut(s) 163
PspXI VCTCGAGB 1 cut(s) 191
PstI CTGCAG 1 cut(s) 733
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 1 cut(s) 568
RsaI GTAC 2 cut(s) 221, 752
RsaNI GTAC 2 cut(s) 220, 751
SaqAI TTAA 4 cut(s) 456, 543, 608, 834
SatI GCNGC 2 cut(s) 149, 400
Sau3AI GATC 3 cut(s) 278, 390, 568
Sau96I GGNCC 5 cut(s) 4, 57, 163, 241, 242
ScaI AGTACT 1 cut(s) 752
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 3 cut(s) 61, 97, 325
SduI GDGCHC 5 cut(s) 114, 198, 204, 245, 371
SfaNI GCATC 6 cut(s) 117, 178, 406, 428, 456, 634
SfcI CTRYAG 1 cut(s) 729
Sfr274I CTCGAG 1 cut(s) 191
SinI GGWCC 2 cut(s) 4, 163
SlaI CTCGAG 1 cut(s) 191
SmlI CTYRAG 4 cut(s) 191, 377, 621, 746
SmoI CTYRAG 4 cut(s) 191, 377, 621, 746
Sse9I AATT 5 cut(s) 520, 559, 631, 648, 844
SseBI AGGCCT 1 cut(s) 714
SsiI CCGC 1 cut(s) 167
SspMI CTAG 1 cut(s) 858
StuI AGGCCT 1 cut(s) 714
StyD4I CCNGG 3 cut(s) 59, 95, 323
StyI CCWWGG 5 cut(s) 105, 423, 696, 715, 857
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 1 cut(s) 75
TaqI TCGA 3 cut(s) 192, 264, 389
TaqII GACCGA 1 cut(s) 21
TasI AATT 5 cut(s) 520, 559, 631, 648, 844
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 5 cut(s) 266, 316, 341, 667, 933
Tru1I TTAA 4 cut(s) 456, 543, 608, 834
Tru9I TTAA 4 cut(s) 456, 543, 608, 834
TscAI CASTG 1 cut(s) 239
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 2 cut(s) 148, 399
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 3 cut(s) 33, 354, 498
TspGWI ACGGA 1 cut(s) 562
TspRI CASTG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 4, 163
XapI RAATTY 3 cut(s) 559, 631, 844
XcmI CCANNNNNNNNNTGG 1 cut(s) 51
XhoI CTCGAG 1 cut(s) 191
XmaJI CCTAGG 1 cut(s) 857
XspI CTAG 1 cut(s) 858
ZrmI AGTACT 1 cut(s) 752
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.