Rh2DG011500

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002120
Physical Location & Seq
Reverse (-)
649027 .. 652036
3010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG011500.1

Sequence Viewer

Length: 930 bp
ATGGACCGAACCAATAATGAAGAAGAAGAAGAAGAACCACCAGTCTGCGACTGGGCCGGGTTGACCTACGCGTGCCTCGCCAACATCCTCGCCCGGCTTCCCCTGGAGCACCGATGGCGTGCGATGCTCCTCTGCAAATCATGGCTGCTCGCCTGCAAGGACCCGCATCTCAACTCGCGCTTCGTCCTCGAGCCCGAGCCCAAGCTCGAGTACGGGTCGGCTCAGTGGTGGGGCCCCGAGTTGGGGAGAAGGGTGGATTCCATTCTCCGATCTGTTGCTGAATGGAGCGAGGGGTCGCTTAGGGAGATTCGCACCCGGAACTGCTCTAATGAATCTCTCCATTTCGTCGCCCAAAGGTGCCCAAACCTTGAGGTTCTTTCGATCAAGTGCTGCCCGAATGTGACTGATGCATCCATGGCTGACATAGCGTTTCGGTGCATCATGATTAAGGAACTTGACATCAGCTATTGCCATGAAATATCTCACGAGTCTCTGGTGTTGATTGGGAAGAATTGCCCTAATCTCACAGTTGTTAAACGGAATGCGTTGAATTTGCTGGATCCTTCTGAACACGCTGGGATTATTCCTGAGGAGTATTTAAAAACTTGCCCTCAAGATGGAAATTCAGAAGCTGATGCAATTGGCAAATCTATGCCGAATCTCAAGCACCTTGAACTAAGGTTCTCCAAGGTGACAGCTAAAGGCCTTGGTTTGATATCTGCAGGGTGTCTGAAACTTGAGTACTTGGATGTGTTTGGGTGTGTAAACTTGAATAGTCGTGATATCAACAATGCAACATCTGAGCTGAAGAACTTGAAGGAGATTAAAAAGCCAAATTTCTACATCCCTAGGTCGGTTTTCCATTCGGAGAGGTATGGACATTGGAGGCTGTATGATGAGAGATTTCAAACAGATATTTTCAGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

309

Amino Acids

35.35

Weight (kDa)

6.03

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 357
AccII CGCG 2 cut(s) 71, 178
AciI CCGC 1 cut(s) 164
AclWI GGATC 2 cut(s) 554, 567
AcsI RAATTY 3 cut(s) 550, 622, 835
AcuI CTGAAG 1 cut(s) 827
AfaI GTAC 2 cut(s) 212, 743
AfiI CCNNNNNNNGG 5 cut(s) 241, 242, 243, 617, 853
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 5 cut(s) 550, 674, 772, 817, 908
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 5 cut(s) 205, 465, 632, 698, 805
AluI AGCT 5 cut(s) 205, 465, 632, 698, 805
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 2 cut(s) 554, 567
AlwNI CAGNNNCTG 1 cut(s) 632
Ama87I CYCGRG 4 cut(s) 188, 194, 206, 236
AoxI GGCC 3 cut(s) 54, 232, 703
ApaI GGGCCC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 145, 390
ApoI RAATTY 3 cut(s) 550, 622, 835
AspA2I CCTAGG 1 cut(s) 848
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 5 cut(s) 4, 54, 160, 232, 233
AsuC2I CCSGG 3 cut(s) 58, 94, 316
AsuHPI GGTGA 1 cut(s) 703
AvaI CYCGRG 4 cut(s) 188, 194, 206, 236
AvaII GGWCC 2 cut(s) 4, 160
AvrII CCTAGG 1 cut(s) 848
AxyI CCTNAGG 1 cut(s) 588
BaeGI GKGCMC 2 cut(s) 236, 362
BamHI GGATCC 1 cut(s) 559
BanI GGYRCC 1 cut(s) 357
BanII GRGCYC 3 cut(s) 195, 201, 236
BauI CACGAG 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 132, 377
BccI CCATC 2 cut(s) 108, 611
BciT130I CCWGG 1 cut(s) 104
BcnI CCSGG 3 cut(s) 58, 94, 316
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 1 cut(s) 849
BfmI CTRYAG 1 cut(s) 720
BisI GCNGC 2 cut(s) 146, 391
BlnI CCTAGG 1 cut(s) 848
BlsI GCNGC 2 cut(s) 147, 392
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 4 cut(s) 58, 94, 104, 316
Bme18I GGWCC 2 cut(s) 4, 160
BmeT110I CYCGRG 4 cut(s) 188, 194, 206, 236
BmgT120I GGNCC 5 cut(s) 4, 54, 160, 232, 233
BmiI GGNNCC 6 cut(s) 162, 233, 234, 235, 359, 561
BmrFI CCNGG 4 cut(s) 58, 94, 104, 316
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 6 cut(s) 114, 175, 397, 419, 447, 625
BmuI ACTGGG 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 125
Bpu10I CCTNAGC 1 cut(s) 299
BpuEI CTTGAG 4 cut(s) 389, 597, 647, 758
BpuMI CCSGG 3 cut(s) 58, 94, 316
BsaJI CCNNGG 5 cut(s) 102, 414, 687, 706, 848
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 242, 243, 617, 853
Bse1I ACTGG 2 cut(s) 41, 56
Bse21I CCTNAGG 1 cut(s) 588
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 5 cut(s) 102, 414, 687, 706, 848
BseGI GGATG 4 cut(s) 84, 410, 754, 843
BseLI CCNNNNNNNGG 5 cut(s) 241, 242, 243, 617, 853
BseMII CTCAG 3 cut(s) 236, 579, 792
BseNI ACTGG 2 cut(s) 41, 56
BseRI GAGGAG 2 cut(s) 119, 605
BseSI GKGCMC 2 cut(s) 236, 362
BseXI GCAGC 2 cut(s) 132, 377
BseYI CCCAGC 1 cut(s) 575
Bsh1236I CGCG 2 cut(s) 71, 178
BshFI GGCC 3 cut(s) 56, 234, 705
BshNI GGYRCC 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 111
BsiHKCI CYCGRG 4 cut(s) 188, 194, 206, 236
BsiSI CCGG 3 cut(s) 57, 94, 316
BslI CCNNNNNNNGG 5 cut(s) 241, 242, 243, 617, 853
BsmAI GTCTC 1 cut(s) 495
BsmI GAATGC 1 cut(s) 547
BsnI GGCC 3 cut(s) 56, 234, 705
BsoBI CYCGRG 4 cut(s) 188, 194, 206, 236
Bsp120I GGGCCC 1 cut(s) 232
Bsp1286I GDGCHC 5 cut(s) 111, 195, 201, 236, 362
Bsp143I GATC 3 cut(s) 269, 381, 559
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 1 cut(s) 164
BspANI GGCC 3 cut(s) 56, 234, 705
BspCNI CTCAG 3 cut(s) 235, 580, 793
BspFNI CGCG 2 cut(s) 71, 178
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 6 cut(s) 162, 233, 234, 235, 359, 561
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 2 cut(s) 554, 567
BspT107I GGYRCC 1 cut(s) 357
BsrI ACTGG 2 cut(s) 41, 56
BssECI CCNNGG 5 cut(s) 102, 414, 687, 706, 848
BssMI GATC 3 cut(s) 269, 381, 559
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 4 cut(s) 414, 687, 706, 848
Bst2BI CACGAG 1 cut(s) 485
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 529
BstC8I GCNNGC 4 cut(s) 73, 120, 150, 154
BstDEI CTNAG 5 cut(s) 222, 299, 588, 677, 801
BstDSI CCRYGG 1 cut(s) 414
BstF5I GGATG 4 cut(s) 84, 410, 754, 843
BstFNI CGCG 2 cut(s) 71, 178
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 3 cut(s) 272, 384, 562
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 3 cut(s) 269, 381, 559
BstMWI GCNNNNNNNGC 5 cut(s) 77, 115, 124, 416, 425
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 4 cut(s) 56, 92, 102, 314
BstSFI CTRYAG 1 cut(s) 720
BstSLI GKGCMC 2 cut(s) 236, 362
BstUI CGCG 2 cut(s) 71, 178
BstV1I GCAGC 2 cut(s) 132, 377
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
Bsu36I CCTNAGG 1 cut(s) 588
BsuRI GGCC 3 cut(s) 56, 234, 705
BtgI CCRYGG 1 cut(s) 414
BtgZI GCGATG 1 cut(s) 137
BtsCI GGATG 4 cut(s) 84, 410, 754, 843
BtsIMutI CAGTG 1 cut(s) 230
Cac8I GCNNGC 4 cut(s) 73, 120, 150, 154
CaiI CAGNNNCTG 1 cut(s) 632
CciI TCATGA 1 cut(s) 441
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 5 cut(s) 4, 54, 160, 232, 233
Csp6I GTAC 2 cut(s) 211, 742
CviAII CATG 4 cut(s) 141, 415, 442, 473
CviQI GTAC 2 cut(s) 211, 742
DdeI CTNAG 5 cut(s) 222, 299, 588, 677, 801
DpnI GATC 3 cut(s) 271, 383, 561
DpnII GATC 3 cut(s) 269, 381, 559
DraI TTTAAA 1 cut(s) 600
Eco130I CCWWGG 4 cut(s) 414, 687, 706, 848
Eco147I AGGCCT 1 cut(s) 705
Eco24I GRGCYC 3 cut(s) 195, 201, 236
Eco32I GATATC 2 cut(s) 717, 784
Eco47I GGWCC 2 cut(s) 4, 160
Eco57I CTGAAG 1 cut(s) 827
Eco81I CCTNAGG 1 cut(s) 588
Eco88I CYCGRG 4 cut(s) 188, 194, 206, 236
EcoO109I RGGNCCY 3 cut(s) 160, 232, 233
EcoRII CCWGG 1 cut(s) 102
EcoRV GATATC 2 cut(s) 717, 784
EcoT14I CCWWGG 4 cut(s) 414, 687, 706, 848
EcoT22I ATGCAT 1 cut(s) 412
EcoT38I GRGCYC 3 cut(s) 195, 201, 236
ErhI CCWWGG 4 cut(s) 414, 687, 706, 848
FaeI CATG 4 cut(s) 144, 418, 445, 476
FaiI YATR 8 cut(s) 142, 416, 425, 443, 474, 653, 876, 894
FatI CATG 4 cut(s) 140, 414, 441, 472
FauI CCCGC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 146, 391
FokI GGATG 4 cut(s) 71, 397, 761, 830
FriOI GRGCYC 3 cut(s) 195, 201, 236
Fsp4HI GCNGC 2 cut(s) 146, 391
FspBI CTAG 1 cut(s) 849
GlaI GCGC 1 cut(s) 179
GluI GCNGC 2 cut(s) 146, 391
GsaI CCCAGC 1 cut(s) 579
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 3 cut(s) 56, 234, 705
HapII CCGG 3 cut(s) 57, 94, 316
HhaI GCGC 1 cut(s) 180
Hin1II CATG 4 cut(s) 144, 418, 445, 476
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 6 cut(s) 257, 307, 332, 488, 658, 924
HpaII CCGG 3 cut(s) 57, 94, 316
HphI GGTGA 1 cut(s) 703
Hpy166II GTNNAC 2 cut(s) 63, 766
Hpy188I TCNGA 7 cut(s) 269, 568, 628, 732, 802, 868, 923
Hpy188III TCNNGA 5 cut(s) 442, 485, 587, 614, 779
Hpy8I GTNNAC 2 cut(s) 63, 766
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 3 cut(s) 243, 573, 811
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4V TGCA 7 cut(s) 135, 156, 410, 438, 638, 722, 794
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 115, 124, 416, 425
HpyF3I CTNAG 5 cut(s) 222, 299, 588, 677, 801
Hsp92II CATG 4 cut(s) 144, 418, 445, 476
HspAI GCGC 1 cut(s) 178
Kzo9I GATC 3 cut(s) 269, 381, 559
LmnI GCTCC 3 cut(s) 106, 132, 285
Lsp1109I GCAGC 2 cut(s) 132, 377
LweI GCATC 6 cut(s) 114, 175, 397, 419, 447, 625
MaeI CTAG 1 cut(s) 849
MaeIII GTNAC 2 cut(s) 400, 691
MalI GATC 3 cut(s) 271, 383, 561
MboI GATC 3 cut(s) 269, 381, 559
MboII GAAGA 7 cut(s) 32, 35, 38, 41, 44, 520, 820
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 5 cut(s) 111, 195, 201, 236, 362
MluCI AATT 5 cut(s) 511, 550, 622, 639, 835
MluI ACGCGT 1 cut(s) 69
MlyI GAGTC 1 cut(s) 497
Mph1103I ATGCAT 1 cut(s) 412
MseI TTAA 4 cut(s) 447, 534, 599, 825
MspI CCGG 3 cut(s) 57, 94, 316
MspR9I CCNGG 4 cut(s) 58, 94, 104, 316
MunI CAATTG 1 cut(s) 639
Mva1269I GAATGC 1 cut(s) 547
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 2 cut(s) 71, 178
MwoI GCNNNNNNNGC 5 cut(s) 77, 115, 124, 416, 425
NciI CCSGG 3 cut(s) 58, 94, 316
NcoI CCATGG 1 cut(s) 414
NdeII GATC 3 cut(s) 269, 381, 559
NlaIII CATG 4 cut(s) 144, 418, 445, 476
NlaIV GGNNCC 6 cut(s) 162, 233, 234, 235, 359, 561
NmuCI GTSAC 2 cut(s) 400, 691
NsiI ATGCAT 1 cut(s) 412
PaeR7I CTCGAG 2 cut(s) 188, 206
PagI TCATGA 1 cut(s) 441
PceI AGGCCT 1 cut(s) 705
PctI GAATGC 1 cut(s) 547
PfeI GAWTC 5 cut(s) 257, 307, 332, 658, 924
PkrI GCNGC 2 cut(s) 147, 392
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
PpuMI RGGWCCY 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 575
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 6 cut(s) 162, 233, 234, 235, 359, 561
PspOMI GGGCCC 1 cut(s) 232
PspPI GGNCC 5 cut(s) 4, 54, 160, 232, 233
PspPPI RGGWCCY 1 cut(s) 160
PspXI VCTCGAGB 2 cut(s) 188, 206
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 2 cut(s) 212, 743
RsaNI GTAC 2 cut(s) 211, 742
SaqAI TTAA 4 cut(s) 447, 534, 599, 825
SatI GCNGC 2 cut(s) 146, 391
Sau3AI GATC 3 cut(s) 269, 381, 559
Sau96I GGNCC 5 cut(s) 4, 54, 160, 232, 233
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 4 cut(s) 58, 94, 104, 316
SduI GDGCHC 5 cut(s) 111, 195, 201, 236, 362
SfaNI GCATC 6 cut(s) 114, 175, 397, 419, 447, 625
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 2 cut(s) 188, 206
SinI GGWCC 2 cut(s) 4, 160
SlaI CTCGAG 2 cut(s) 188, 206
SmlI CTYRAG 6 cut(s) 188, 206, 368, 612, 662, 737
SmoI CTYRAG 6 cut(s) 188, 206, 368, 612, 662, 737
Sse9I AATT 5 cut(s) 511, 550, 622, 639, 835
SseBI AGGCCT 1 cut(s) 705
SsiI CCGC 1 cut(s) 164
SspMI CTAG 1 cut(s) 849
StuI AGGCCT 1 cut(s) 705
StyD4I CCNGG 4 cut(s) 56, 92, 102, 314
StyI CCWWGG 4 cut(s) 414, 687, 706, 848
TaaI ACNGT 1 cut(s) 529
TaqI TCGA 3 cut(s) 189, 207, 380
TaqII GACCGA 1 cut(s) 21
TasI AATT 5 cut(s) 511, 550, 622, 639, 835
TatI WGTACW 1 cut(s) 741
TfiI GAWTC 5 cut(s) 257, 307, 332, 658, 924
Tru1I TTAA 4 cut(s) 447, 534, 599, 825
Tru9I TTAA 4 cut(s) 447, 534, 599, 825
TscAI CASTG 1 cut(s) 230
TseFI GTSAC 2 cut(s) 400, 691
TseI GCWGC 2 cut(s) 145, 390
Tsp45I GTSAC 2 cut(s) 400, 691
TspDTI ATGAA 3 cut(s) 33, 345, 489
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 4, 160
XapI RAATTY 3 cut(s) 550, 622, 835
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
XhoI CTCGAG 2 cut(s) 188, 206
XmaJI CCTAGG 1 cut(s) 848
XspI CTAG 1 cut(s) 849
ZrmI AGTACT 1 cut(s) 743
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.