Rorug05G0551200

F-box LRR-repeat protein 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002120
Physical Location & Seq
Forward (+)
73932436 .. 73935831
3396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0551200.1

Sequence Viewer

Length: 1584 bp
ATGGATAACAACAATGGCATTCGTCCTCAAGATGGACAGCCCTCAATGGAAGCAGGAGATTGGAGGAGCAGAATGCCCACGGATATGCGGCAAAGACAGATAATGTCTATATTGAAGGCGCATCGCCCCATTTCTGGTCAAGAGGGATTAGATGAAGTCGAGAAAAATGCTGCAAGTTTGGAGGAAAAGATTTATGCTGCTGCCACTAGCCGGTTGGACTATCTAGAGAAAATTTCTCAGGGGATACACACAGTTCTGACCAAGCCACAGAATACAACATCCAATCCTCTAGAATCCAATGGCAAGGGACTTCTCGATTCAGTTGCTGATCAAGGACAATCAGTCTCAATGTCATTGCCAGCCAATCAGTCCCAAACAAGGCAACATTTCGTACCACAGAACATACAGAGTAACATTCTGCCTGCCGGAGTTCAAAGTTATGCTGGGTTGTCATCTGCACTTCCTCCTACCACGAACAATCAAACAAGGGAACAATTTGTACCGCAAAATATTCAGAATAGCATTCTGACTGCTGGAGTTCAAAGTTCCGTTGGCTTATCATCTGCACTATCTCCTACCGGGAACTATCATCTGTATTGCCTCCAACCCCCTGCCCCTGGTCAAAATGTTACCGGCTTATCATCTGCACTACCTCCTCCACAGGGTCTGAATATGTCATCTGCACTACTTGCCTCTATTCCATCTGCCTTTTCTTCTGCCCCAGCATCTATTATGCGACCTTCCACACTGCAGTCATCTCTCCCTAGGCTTCAGCAGAATCAACAATCAACTATTCCCCACTCTTCACAATCCATGCTTCAGCAAACAGCAGTGCCACAACAGCAAATGTTGCCCCAACAGCATCAACAGCCACAGTTAACAGGGTCTTCAACAGCTCAGCAGAACAAGAAGAATCCATCGCAGTCGACTGCAGAGCTGTTAACCACAATAAAGAACTCAGAGCAGCAGGTCCCTATGGGCTCTATTCAACAAACTCCGGTCATTGCTCCCCAGCAAGCTAACATGAATGTACAGAGTGGGATTAACACATTGCACCCAAACGTTATTCCACTCGGTATTCTTCAGAACCAACAACAATATCAGAATCAACTTCAGCAACAATATCAAATCCAGCTACTATATCAACAGTGTCAACAGCTGCAGTTGATGCAGATGCAGCAGATAAATCAACAACTGCAACAGCAGCTGGCTAGACAGTCGCAGACACAACAAATGCCACAGCTTCACCAAATCAGAGTTGTTGACGCGAACATGAATGCCTCGTCATCACAGAGTGGGAGTAATATGCTGCAGGCAAACATTGTTTCTCCTCCGCCAAATTCTGGTATTGTTGATAACCAGCATCTGAAGCAAGAACAGGAGCAGAAAATGTTTAAGCATCAACCCGAGCAACCATATCAACAGCTACAGATGCAGAAGCCGCAGATGCTTCAAGAAAAGCAACAGCAAAAGATACAGCAACTAGAGCAACAAGCAAAGCAGCAGCTGTGTCATATTTATTTCATTTATCTCATCTTTCTCACCATTCTGATGAGTGCTCTCTTCTTCGGGATAGGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

527

Amino Acids

58.4

Weight (kDa)

8.64

Isoelectric Point (pI)

77.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 95 9.4e-16 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 958
AccB7I CCANNNNNTGG 1 cut(s) 1343
AccI GTMKAC 1 cut(s) 926
AccII CGCG 1 cut(s) 1268
AciI CCGC 4 cut(s) 88, 503, 1334, 1442
AclI AACGTT 1 cut(s) 1062
AcsI RAATTY 2 cut(s) 231, 1339
AcuI CTGAAG 5 cut(s) 755, 803, 1067, 1097, 1388
AdeI CACNNNGTG 1 cut(s) 1295
AfaI GTAC 3 cut(s) 393, 501, 1032
AfiI CCNNNNNNNGG 7 cut(s) 32, 134, 210, 378, 617, 662, 1343
AgsI TTSAA 6 cut(s) 115, 434, 542, 891, 989, 1454
AhdI GACNNNNNGTC 1 cut(s) 341
AjnI CCWGG 1 cut(s) 616
AluBI AGCT 9 cut(s) 896, 937, 1019, 1135, 1159, 1207, 1243, 1426, 1507
AluI AGCT 9 cut(s) 896, 937, 1019, 1135, 1159, 1207, 1243, 1426, 1507
Alw21I GWGCWC 1 cut(s) 1561
Alw26I GTCTC 1 cut(s) 349
AlwNI CAGNNNCTG 5 cut(s) 326, 667, 1207, 1366, 1507
Ama87I CYCGRG 1 cut(s) 1406
AoxI GGCC 1 cut(s) 1576
ApoI RAATTY 2 cut(s) 231, 1339
AspA2I CCTAGG 1 cut(s) 764
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 1 cut(s) 970
AsuC2I CCSGG 1 cut(s) 580
AsuHPI GGTGA 2 cut(s) 1238, 1534
AvaI CYCGRG 1 cut(s) 1406
AvaII GGWCC 1 cut(s) 970
AvrII CCTAGG 1 cut(s) 764
BanII GRGCYC 1 cut(s) 983
BbsI GAAGAC 1 cut(s) 879
Bbv12I GWGCWC 1 cut(s) 1561
BccI CCATC 3 cut(s) 26, 709, 925
BcgI CGANNNNNNTGC 4 cut(s) 149, 183, 305, 339
BciT130I CCWGG 1 cut(s) 618
BciVI GTATCC 1 cut(s) 237
BclI TGATCA 1 cut(s) 328
BcnI CCSGG 1 cut(s) 580
BcoDI GTCTC 1 cut(s) 349
BfaI CTAG 6 cut(s) 207, 224, 290, 765, 1212, 1484
BfmI CTRYAG 5 cut(s) 749, 930, 1160, 1310, 1427
BfuAI ACCTGC 1 cut(s) 958
BfuI GTATCC 1 cut(s) 237
BlnI CCTAGG 1 cut(s) 764
BlpI GCTNAGC 1 cut(s) 897
Bme1390I CCNGG 2 cut(s) 580, 618
Bme18I GGWCC 1 cut(s) 970
BmeRI GACNNNNNGTC 1 cut(s) 341
BmeT110I CYCGRG 1 cut(s) 1406
BmgT120I GGNCC 1 cut(s) 970
BmiI GGNNCC 1 cut(s) 972
BmrFI CCNGG 2 cut(s) 580, 618
BmsI GCATC 9 cut(s) 130, 734, 871, 1158, 1164, 1372, 1408, 1422, 1437
BpiI GAAGAC 1 cut(s) 879
BpmI CTGGAG 1 cut(s) 555
Bpu1102I GCTNAGC 1 cut(s) 897
BpuEI CTTGAG 1 cut(s) 12
BpuMI CCSGG 1 cut(s) 580
BsaJI CCNNGG 3 cut(s) 78, 616, 764
BsaWI WCCGGW 1 cut(s) 997
Bsc4I CCNNNNNNNGG 7 cut(s) 32, 134, 210, 378, 617, 662, 1343
Bse118I RCCGGY 2 cut(s) 210, 632
Bse3DI GCAATG 3 cut(s) 353, 1002, 1049
BseBI CCWGG 1 cut(s) 618
BseDI CCNNGG 3 cut(s) 78, 616, 764
BseGI GGATG 1 cut(s) 278
BseLI CCNNNNNNNGG 7 cut(s) 32, 134, 210, 378, 617, 662, 1343
BseMI GCAATG 3 cut(s) 353, 1002, 1049
BseMII CTCAG 3 cut(s) 251, 911, 972
BseRI GAGGAG 3 cut(s) 79, 645, 1320
BseYI CCCAGC 3 cut(s) 443, 721, 1011
BsgI GTGCAG 4 cut(s) 441, 549, 630, 666
Bsh1236I CGCG 1 cut(s) 1268
BshFI GGCC 1 cut(s) 1578
BsiHKAI GWGCWC 1 cut(s) 1561
BsiHKCI CYCGRG 1 cut(s) 1406
BsiSI CCGG 5 cut(s) 211, 426, 579, 633, 998
BslFI GGGAC 3 cut(s) 321, 355, 956
BslI CCNNNNNNNGG 7 cut(s) 32, 134, 210, 378, 617, 662, 1343
BsmAI GTCTC 1 cut(s) 349
BsmFI GGGAC 3 cut(s) 321, 355, 956
BsmI GAATGC 4 cut(s) 18, 78, 522, 1282
BsnI GGCC 1 cut(s) 1578
BsoBI CYCGRG 1 cut(s) 1406
Bsp1286I GDGCHC 2 cut(s) 983, 1561
Bsp1407I TGTACA 1 cut(s) 1030
Bsp143I GATC 1 cut(s) 328
Bsp1720I GCTNAGC 1 cut(s) 897
BspACI CCGC 4 cut(s) 88, 503, 1334, 1442
BspANI GGCC 1 cut(s) 1578
BspCNI CTCAG 3 cut(s) 250, 910, 971
BspFNI CGCG 1 cut(s) 1268
BspLI GGNNCC 1 cut(s) 972
BspMAI CTGCAG 4 cut(s) 753, 934, 1164, 1314
BspMI ACCTGC 1 cut(s) 958
BsrDI GCAATG 3 cut(s) 353, 1002, 1049
BsrFI RCCGGY 2 cut(s) 210, 632
BsrGI TGTACA 1 cut(s) 1030
BssAI RCCGGY 2 cut(s) 210, 632
BssECI CCNNGG 3 cut(s) 78, 616, 764
BssMI GATC 1 cut(s) 328
BssT1I CCWWGG 1 cut(s) 764
Bst2UI CCWGG 1 cut(s) 618
Bst4CI ACNGT 4 cut(s) 253, 876, 1149, 1218
Bst6I CTCTTC 2 cut(s) 808, 1568
BstAPI GCANNNNNTGC 3 cut(s) 689, 850, 1168
BstAUI TGTACA 1 cut(s) 1030
BstC8I GCNNGC 5 cut(s) 360, 423, 1017, 1209, 1314
BstDEI CTNAG 3 cut(s) 237, 897, 958
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 1 cut(s) 278
BstFNI CGCG 1 cut(s) 1268
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 1 cut(s) 349
BstMBI GATC 1 cut(s) 328
BstNI CCWGG 1 cut(s) 618
BstSCI CCNGG 2 cut(s) 578, 616
BstSFI CTRYAG 5 cut(s) 749, 930, 1160, 1310, 1427
BstUI CGCG 1 cut(s) 1268
BstV2I GAAGAC 1 cut(s) 879
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 1 cut(s) 1578
BtgI CCRYGG 1 cut(s) 78
BtgZI GCGATG 2 cut(s) 107, 903
BtsCI GGATG 1 cut(s) 278
BtsI GCAGTG 2 cut(s) 746, 837
BtsIMutI CAGTG 3 cut(s) 746, 837, 1154
BveI ACCTGC 1 cut(s) 958
Cac8I GCNNGC 5 cut(s) 360, 423, 1017, 1209, 1314
CaiI CAGNNNCTG 5 cut(s) 326, 667, 1207, 1366, 1507
CfoI GCGC 1 cut(s) 121
Cfr10I RCCGGY 2 cut(s) 210, 632
Cfr13I GGNCC 1 cut(s) 970
CseI GACGC 1 cut(s) 1274
Csp6I GTAC 3 cut(s) 392, 500, 1031
CviAII CATG 3 cut(s) 814, 1024, 1273
CviQI GTAC 3 cut(s) 392, 500, 1031
DdeI CTNAG 3 cut(s) 237, 897, 958
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
DraIII CACNNNGTG 1 cut(s) 1295
DriI GACNNNNNGTC 1 cut(s) 341
Eam1104I CTCTTC 2 cut(s) 808, 1568
Eam1105I GACNNNNNGTC 1 cut(s) 341
EarI CTCTTC 2 cut(s) 808, 1568
EciI GGCGGA 1 cut(s) 1323
Eco130I CCWWGG 1 cut(s) 764
Eco24I GRGCYC 1 cut(s) 983
Eco47I GGWCC 1 cut(s) 970
Eco57I CTGAAG 5 cut(s) 755, 803, 1067, 1097, 1388
Eco88I CYCGRG 1 cut(s) 1406
EcoO109I RGGNCCY 1 cut(s) 970
EcoRII CCWGG 1 cut(s) 616
EcoT14I CCWWGG 1 cut(s) 764
EcoT38I GRGCYC 1 cut(s) 983
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 3 cut(s) 817, 1027, 1276
FaqI GGGAC 3 cut(s) 321, 355, 956
FatI CATG 3 cut(s) 813, 1023, 1272
FbaI TGATCA 1 cut(s) 328
FblI GTMKAC 1 cut(s) 926
FokI GGATG 1 cut(s) 265
FriOI GRGCYC 1 cut(s) 983
FspBI CTAG 6 cut(s) 207, 224, 290, 765, 1212, 1484
GlaI GCGC 1 cut(s) 120
GsaI CCCAGC 3 cut(s) 447, 725, 1015
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 1578
HapII CCGG 5 cut(s) 211, 426, 579, 633, 998
HgaI GACGC 1 cut(s) 1274
HhaI GCGC 1 cut(s) 121
Hin1II CATG 3 cut(s) 817, 1027, 1276
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HincII GTYRAC 5 cut(s) 879, 927, 942, 1154, 1264
HindII GTYRAC 5 cut(s) 879, 927, 942, 1154, 1264
HinfI GANTC 5 cut(s) 293, 317, 778, 913, 1105
HpaI GTTAAC 2 cut(s) 879, 942
HpaII CCGG 5 cut(s) 211, 426, 579, 633, 998
HphI GGTGA 2 cut(s) 1238, 1534
Hpy166II GTNNAC 5 cut(s) 879, 927, 942, 1154, 1264
Hpy188III TCNNGA 8 cut(s) 29, 140, 160, 224, 290, 314, 1454, 1570
Hpy8I GTNNAC 5 cut(s) 879, 927, 942, 1154, 1264
HpyAV CCTTC 2 cut(s) 109, 750
HpyCH4III ACNGT 4 cut(s) 253, 876, 1149, 1218
HpyCH4IV ACGT 1 cut(s) 1062
HpyF3I CTNAG 3 cut(s) 237, 897, 958
HpySE526I ACGT 1 cut(s) 1062
Hsp92II CATG 3 cut(s) 817, 1027, 1276
HspAI GCGC 1 cut(s) 119
Ksp22I TGATCA 1 cut(s) 328
KspAI GTTAAC 2 cut(s) 879, 942
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 3 cut(s) 66, 1012, 1381
LweI GCATC 9 cut(s) 130, 734, 871, 1158, 1164, 1372, 1408, 1422, 1437
MaeI CTAG 6 cut(s) 207, 224, 290, 765, 1212, 1484
MaeII ACGT 1 cut(s) 1062
MaeIII GTNAC 2 cut(s) 410, 628
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 7 cut(s) 705, 795, 879, 922, 1073, 1555, 1558
MhlI GDGCHC 2 cut(s) 983, 1561
MluCI AATT 3 cut(s) 231, 494, 1339
MmeI TCCRAC 2 cut(s) 195, 628
MseI TTAA 4 cut(s) 878, 941, 1044, 1395
MslI CAYNNNNRTG 2 cut(s) 83, 1550
MspA1I CMGCKG 3 cut(s) 1159, 1207, 1507
MspI CCGG 5 cut(s) 211, 426, 579, 633, 998
MspR9I CCNGG 2 cut(s) 580, 618
Mva1269I GAATGC 4 cut(s) 18, 78, 522, 1282
MvaI CCWGG 1 cut(s) 618
MvnI CGCG 1 cut(s) 1268
NciI CCSGG 1 cut(s) 580
NdeII GATC 1 cut(s) 328
NlaIII CATG 3 cut(s) 817, 1027, 1276
NlaIV GGNNCC 1 cut(s) 972
PctI GAATGC 4 cut(s) 18, 78, 522, 1282
PfeI GAWTC 5 cut(s) 293, 317, 778, 913, 1105
PflMI CCANNNNNTGG 1 cut(s) 1343
PpuMI RGGWCCY 1 cut(s) 970
Psp1406I AACGTT 1 cut(s) 1062
Psp5II RGGWCCY 1 cut(s) 970
Psp6I CCWGG 1 cut(s) 616
PspFI CCCAGC 3 cut(s) 443, 721, 1011
PspGI CCWGG 1 cut(s) 616
PspN4I GGNNCC 1 cut(s) 972
PspPI GGNCC 1 cut(s) 970
PspPPI RGGWCCY 1 cut(s) 970
PsrI GAACNNNNNNTAC 2 cut(s) 483, 515
PstI CTGCAG 4 cut(s) 753, 934, 1164, 1314
PstNI CAGNNNCTG 5 cut(s) 326, 667, 1207, 1366, 1507
PvuII CAGCTG 3 cut(s) 1159, 1207, 1507
RsaI GTAC 3 cut(s) 393, 501, 1032
RsaNI GTAC 3 cut(s) 392, 500, 1031
RseI CAYNNNNRTG 2 cut(s) 83, 1550
SalI GTCGAC 1 cut(s) 925
SaqAI TTAA 4 cut(s) 878, 941, 1044, 1395
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 1 cut(s) 970
ScrFI CCNGG 2 cut(s) 580, 618
SduI GDGCHC 2 cut(s) 983, 1561
SfaNI GCATC 9 cut(s) 130, 734, 871, 1158, 1164, 1372, 1408, 1422, 1437
SfcI CTRYAG 5 cut(s) 749, 930, 1160, 1310, 1427
SinI GGWCC 1 cut(s) 970
SmiMI CAYNNNNRTG 2 cut(s) 83, 1550
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 3 cut(s) 231, 494, 1339
SsiI CCGC 4 cut(s) 88, 503, 1334, 1442
SspI AATATT 1 cut(s) 511
SspMI CTAG 6 cut(s) 207, 224, 290, 765, 1212, 1484
StyD4I CCNGG 2 cut(s) 578, 616
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 4 cut(s) 253, 876, 1149, 1218
TaiI ACGT 1 cut(s) 1065
TaqI TCGA 3 cut(s) 159, 315, 926
TasI AATT 3 cut(s) 231, 494, 1339
TatI WGTACW 1 cut(s) 1030
TauI GCSGC 2 cut(s) 91, 1444
TfiI GAWTC 5 cut(s) 293, 317, 778, 913, 1105
Tru1I TTAA 4 cut(s) 878, 941, 1044, 1395
Tru9I TTAA 4 cut(s) 878, 941, 1044, 1395
TscAI CASTG 3 cut(s) 753, 837, 1154
TspDTI ATGAA 4 cut(s) 168, 1040, 1289, 1513
TspGWI ACGGA 2 cut(s) 95, 538
TspRI CASTG 3 cut(s) 753, 837, 1154
Van91I CCANNNNNTGG 1 cut(s) 1343
VpaK11BI GGWCC 1 cut(s) 970
XapI RAATTY 2 cut(s) 231, 1339
XbaI TCTAGA 2 cut(s) 223, 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmaJI CCTAGG 1 cut(s) 764
XmiI GTMKAC 1 cut(s) 926
XspI CTAG 6 cut(s) 207, 224, 290, 765, 1212, 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.