Rroxscaffold_2G00155350

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002120
Physical Location & Seq
Forward (+)
91732787 .. 91734208
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155350.1

Sequence Viewer

Length: 960 bp
ATGGACCGAACCAATAATAAAAAAGAAGAAGAAGAAGAACCACCAGTGACCGACGAGTCCGACTCCAACTGGGCCGGGTTGACCTACGAGTGCCTCGCCAACATCCTCGCCCGGATCCCCTTGAAGCACCGATGGCGTGCGATGCTCCTCTGCAAATCATGGCTGCTCGCCTGCAAGGACCCACATCTCAACTCGCGCTTCGTCCTCGAGCCCGAGCCAGAGCCCAAGCCCGAGCCCGAGTACGGGTCGGCTCAGTGGTGGGCCCCCGAGTTGGGGAGAAGGGTCGATTCCATTCTCCGATCTGTTGCTGAATGGAGCGAGGGGTCGCTTAGGGAGATTCGCACCCGGAACTGCTCTAATGAGTCTCTCCATTTCGTCGCCCAAAGGTGCCCAAACCTTGAGGTTCTTTCGATCAAGTGCTGCCCGAATGTGACTGATGCATCCATGGCTGACATAGCGTTTCGGTGCACCATGATTAAGGAACTTGACATCAGCTATTGCCATGAAATATCTCACGAGTCTCTGGTGTTGATTGGGAAGAATTGCCCTAATCTCACAATTGTTAAACGGAATGCGTTGAATATGCTAGATCCTTCTGAACACGCTGGGATTGTTCCTGAGGAGTATTTGAAAACTTGCCCTCAAGATGGAAATTCAGAAGCTGATGCAATAGGCAAATCTATGCCGAATCTCAAGCACCTTGAACTAAGGTTCTCCAAGGTAACGGCTAAAGGCCTTGGTTTGATATCTGCAGGGTGTCTGAAACTTGAGTACTTGGATGTGTTTGGGTGTGTAAACTTGAATAGTCGTGATATCAACAATGCAACATCCGAGCTGAAGAACTTGAAGGAGATTAAAAAGCCAAATTTCTACATCCCTAGGTCGGTTTTCCATACGGAGAGGTATGGACATTGGAGGCTGTATGATGAGAGATTTCAAACAGATATTTTCAGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

319

Amino Acids

36.53

Weight (kDa)

6.04

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 26 - 64 4.2e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 55
AccB1I GGYRCC 1 cut(s) 387
AccII CGCG 1 cut(s) 196
AclWI GGATC 3 cut(s) 109, 122, 584
AcsI RAATTY 2 cut(s) 652, 865
AcuI CTGAAG 1 cut(s) 857
AfaI GTAC 2 cut(s) 242, 773
AfiI CCNNNNNNNGG 7 cut(s) 242, 243, 271, 272, 273, 647, 883
AgsI TTSAA 7 cut(s) 124, 580, 631, 704, 802, 847, 938
AluBI AGCT 3 cut(s) 495, 662, 835
AluI AGCT 3 cut(s) 495, 662, 835
Alw21I GWGCWC 1 cut(s) 470
Alw26I GTCTC 2 cut(s) 369, 525
Alw44I GTGCAC 1 cut(s) 466
AlwI GGATC 3 cut(s) 109, 122, 584
AlwNI CAGNNNCTG 1 cut(s) 662
Ama87I CYCGRG 5 cut(s) 206, 212, 230, 236, 266
AoxI GGCC 3 cut(s) 72, 261, 733
ApaI GGGCCC 1 cut(s) 265
ApaLI GTGCAC 1 cut(s) 466
ApeKI GCWGC 2 cut(s) 163, 420
ApoI RAATTY 2 cut(s) 652, 865
AspA2I CCTAGG 1 cut(s) 878
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 5 cut(s) 4, 72, 178, 261, 262
AsuC2I CCSGG 3 cut(s) 76, 112, 346
AvaI CYCGRG 5 cut(s) 206, 212, 230, 236, 266
AvaII GGWCC 2 cut(s) 4, 178
AvrII CCTAGG 1 cut(s) 878
AxyI CCTNAGG 1 cut(s) 618
BaeGI GKGCMC 3 cut(s) 265, 392, 470
BamHI GGATCC 1 cut(s) 114
BanI GGYRCC 1 cut(s) 387
BanII GRGCYC 4 cut(s) 213, 225, 237, 265
BauI CACGAG 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 470
BbvI GCAGC 2 cut(s) 150, 407
BccI CCATC 2 cut(s) 126, 641
BceAI ACGGC 1 cut(s) 741
BcnI CCSGG 3 cut(s) 76, 112, 346
BcoDI GTCTC 2 cut(s) 369, 525
BfaI CTAG 2 cut(s) 587, 879
BfmI CTRYAG 1 cut(s) 750
BisI GCNGC 2 cut(s) 164, 421
BlnI CCTAGG 1 cut(s) 878
BlsI GCNGC 2 cut(s) 165, 422
BmcAI AGTACT 1 cut(s) 773
Bme1390I CCNGG 3 cut(s) 76, 112, 346
Bme18I GGWCC 2 cut(s) 4, 178
BmeT110I CYCGRG 5 cut(s) 206, 212, 230, 236, 266
BmgT120I GGNCC 5 cut(s) 4, 72, 178, 261, 262
BmiI GGNNCC 5 cut(s) 116, 180, 263, 264, 389
BmrFI CCNGG 3 cut(s) 76, 112, 346
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 4 cut(s) 132, 427, 449, 655
BmuI ACTGGG 1 cut(s) 79
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu10I CCTNAGC 1 cut(s) 329
BpuEI CTTGAG 4 cut(s) 419, 627, 677, 788
BpuMI CCSGG 3 cut(s) 76, 112, 346
BsaJI CCNNGG 4 cut(s) 444, 717, 736, 878
Bsc4I CCNNNNNNNGG 7 cut(s) 242, 243, 271, 272, 273, 647, 883
Bse1I ACTGG 2 cut(s) 44, 74
Bse21I CCTNAGG 1 cut(s) 618
BseDI CCNNGG 4 cut(s) 444, 717, 736, 878
BseGI GGATG 5 cut(s) 102, 440, 784, 827, 873
BseLI CCNNNNNNNGG 7 cut(s) 242, 243, 271, 272, 273, 647, 883
BseMII CTCAG 2 cut(s) 266, 609
BseNI ACTGG 2 cut(s) 44, 74
BseRI GAGGAG 2 cut(s) 137, 635
BseSI GKGCMC 3 cut(s) 265, 392, 470
BseXI GCAGC 2 cut(s) 150, 407
BseYI CCCAGC 1 cut(s) 605
Bsh1236I CGCG 1 cut(s) 196
BshFI GGCC 3 cut(s) 74, 263, 735
BshNI GGYRCC 1 cut(s) 387
BsiHKAI GWGCWC 1 cut(s) 470
BsiHKCI CYCGRG 5 cut(s) 206, 212, 230, 236, 266
BsiSI CCGG 3 cut(s) 75, 112, 346
BslI CCNNNNNNNGG 7 cut(s) 242, 243, 271, 272, 273, 647, 883
BsmAI GTCTC 2 cut(s) 369, 525
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 3 cut(s) 74, 263, 735
BsoBI CYCGRG 5 cut(s) 206, 212, 230, 236, 266
Bsp120I GGGCCC 1 cut(s) 261
Bsp1286I GDGCHC 6 cut(s) 213, 225, 237, 265, 392, 470
Bsp143I GATC 4 cut(s) 114, 299, 411, 589
Bsp19I CCATGG 1 cut(s) 444
BspANI GGCC 3 cut(s) 74, 263, 735
BspCNI CTCAG 2 cut(s) 265, 610
BspFNI CGCG 1 cut(s) 196
BspLI GGNNCC 5 cut(s) 116, 180, 263, 264, 389
BspMAI CTGCAG 1 cut(s) 754
BspPI GGATC 3 cut(s) 109, 122, 584
BspT107I GGYRCC 1 cut(s) 387
BsrI ACTGG 2 cut(s) 44, 74
BssECI CCNNGG 4 cut(s) 444, 717, 736, 878
BssMI GATC 4 cut(s) 114, 299, 411, 589
BssSI CACGAG 1 cut(s) 515
BssT1I CCWWGG 4 cut(s) 444, 717, 736, 878
Bst2BI CACGAG 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 138, 168, 172
BstDEI CTNAG 4 cut(s) 252, 329, 618, 707
BstDSI CCRYGG 1 cut(s) 444
BstF5I GGATG 5 cut(s) 102, 440, 784, 827, 873
BstFNI CGCG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 4 cut(s) 117, 302, 414, 592
BstMAI GTCTC 2 cut(s) 369, 525
BstMBI GATC 4 cut(s) 114, 299, 411, 589
BstMWI GCNNNNNNNGC 4 cut(s) 133, 142, 446, 455
BstSCI CCNGG 3 cut(s) 74, 110, 344
BstSFI CTRYAG 1 cut(s) 750
BstSLI GKGCMC 3 cut(s) 265, 392, 470
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 2 cut(s) 150, 407
BstX2I RGATCY 2 cut(s) 114, 589
BstYI RGATCY 2 cut(s) 114, 589
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 3 cut(s) 74, 263, 735
BtgI CCRYGG 1 cut(s) 444
BtgZI GCGATG 1 cut(s) 155
BtsCI GGATG 5 cut(s) 102, 440, 784, 827, 873
BtsIMutI CAGTG 2 cut(s) 51, 260
Cac8I GCNNGC 3 cut(s) 138, 168, 172
CaiI CAGNNNCTG 1 cut(s) 662
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 5 cut(s) 4, 72, 178, 261, 262
Csp6I GTAC 2 cut(s) 241, 772
CviAII CATG 4 cut(s) 159, 445, 472, 503
CviQI GTAC 2 cut(s) 241, 772
DdeI CTNAG 4 cut(s) 252, 329, 618, 707
DpnI GATC 4 cut(s) 116, 301, 413, 591
DpnII GATC 4 cut(s) 114, 299, 411, 589
DrdI GACNNNNNNGTC 1 cut(s) 55
DseDI GACNNNNNNGTC 1 cut(s) 55
Eco130I CCWWGG 4 cut(s) 444, 717, 736, 878
Eco147I AGGCCT 1 cut(s) 735
Eco24I GRGCYC 4 cut(s) 213, 225, 237, 265
Eco32I GATATC 2 cut(s) 747, 814
Eco47I GGWCC 2 cut(s) 4, 178
Eco57I CTGAAG 1 cut(s) 857
Eco81I CCTNAGG 1 cut(s) 618
Eco88I CYCGRG 5 cut(s) 206, 212, 230, 236, 266
EcoO109I RGGNCCY 2 cut(s) 178, 262
EcoRV GATATC 2 cut(s) 747, 814
EcoT14I CCWWGG 4 cut(s) 444, 717, 736, 878
EcoT22I ATGCAT 1 cut(s) 442
EcoT38I GRGCYC 4 cut(s) 213, 225, 237, 265
ErhI CCWWGG 4 cut(s) 444, 717, 736, 878
FaeI CATG 4 cut(s) 162, 448, 475, 506
FatI CATG 4 cut(s) 158, 444, 471, 502
Fnu4HI GCNGC 2 cut(s) 164, 421
FokI GGATG 5 cut(s) 89, 427, 791, 814, 860
FriOI GRGCYC 4 cut(s) 213, 225, 237, 265
Fsp4HI GCNGC 2 cut(s) 164, 421
FspBI CTAG 2 cut(s) 587, 879
GlaI GCGC 1 cut(s) 197
GluI GCNGC 2 cut(s) 164, 421
GsaI CCCAGC 1 cut(s) 609
HaeIII GGCC 3 cut(s) 74, 263, 735
HapII CCGG 3 cut(s) 75, 112, 346
HhaI GCGC 1 cut(s) 198
Hin1II CATG 4 cut(s) 162, 448, 475, 506
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 8 cut(s) 56, 62, 287, 337, 362, 518, 688, 954
HpaII CCGG 3 cut(s) 75, 112, 346
Hpy166II GTNNAC 3 cut(s) 81, 468, 796
Hpy188I TCNGA 7 cut(s) 61, 299, 598, 658, 762, 832, 953
Hpy188III TCNNGA 4 cut(s) 515, 617, 644, 809
Hpy8I GTNNAC 3 cut(s) 81, 468, 796
Hpy99I CGWCG 2 cut(s) 56, 380
HpyAV CCTTC 3 cut(s) 273, 603, 841
HpyCH4V TGCA 7 cut(s) 153, 174, 440, 468, 668, 752, 824
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 142, 446, 455
HpyF3I CTNAG 4 cut(s) 252, 329, 618, 707
Hsp92II CATG 4 cut(s) 162, 448, 475, 506
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 4 cut(s) 114, 299, 411, 589
LmnI GCTCC 2 cut(s) 150, 315
Lsp1109I GCAGC 2 cut(s) 150, 407
LweI GCATC 4 cut(s) 132, 427, 449, 655
MaeI CTAG 2 cut(s) 587, 879
MaeIII GTNAC 3 cut(s) 46, 430, 721
MalI GATC 4 cut(s) 116, 301, 413, 591
MboI GATC 4 cut(s) 114, 299, 411, 589
MboII GAAGA 6 cut(s) 38, 41, 44, 47, 550, 850
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 2 cut(s) 114, 589
MhlI GDGCHC 6 cut(s) 213, 225, 237, 265, 392, 470
MluCI AATT 4 cut(s) 541, 558, 652, 865
MlyI GAGTC 4 cut(s) 56, 65, 371, 527
MmeI TCCRAC 2 cut(s) 84, 90
Mph1103I ATGCAT 1 cut(s) 442
MseI TTAA 3 cut(s) 477, 564, 855
MspI CCGG 3 cut(s) 75, 112, 346
MspR9I CCNGG 3 cut(s) 76, 112, 346
MunI CAATTG 1 cut(s) 558
Mva1269I GAATGC 1 cut(s) 577
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 4 cut(s) 133, 142, 446, 455
NciI CCSGG 3 cut(s) 76, 112, 346
NcoI CCATGG 1 cut(s) 444
NdeII GATC 4 cut(s) 114, 299, 411, 589
NlaIII CATG 4 cut(s) 162, 448, 475, 506
NlaIV GGNNCC 5 cut(s) 116, 180, 263, 264, 389
NmuCI GTSAC 2 cut(s) 46, 430
NsiI ATGCAT 1 cut(s) 442
PaeR7I CTCGAG 1 cut(s) 206
PceI AGGCCT 1 cut(s) 735
PctI GAATGC 1 cut(s) 577
PfeI GAWTC 4 cut(s) 287, 337, 688, 954
PkrI GCNGC 2 cut(s) 165, 422
PleI GAGTC 4 cut(s) 56, 64, 370, 526
PpsI GAGTC 4 cut(s) 56, 64, 370, 526
PpuMI RGGWCCY 1 cut(s) 178
Psp5II RGGWCCY 1 cut(s) 178
PspFI CCCAGC 1 cut(s) 605
PspN4I GGNNCC 5 cut(s) 116, 180, 263, 264, 389
PspOMI GGGCCC 1 cut(s) 261
PspPI GGNCC 5 cut(s) 4, 72, 178, 261, 262
PspPPI RGGWCCY 1 cut(s) 178
PspXI VCTCGAGB 1 cut(s) 206
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 2 cut(s) 114, 589
RsaI GTAC 2 cut(s) 242, 773
RsaNI GTAC 2 cut(s) 241, 772
SaqAI TTAA 3 cut(s) 477, 564, 855
SatI GCNGC 2 cut(s) 164, 421
Sau3AI GATC 4 cut(s) 114, 299, 411, 589
Sau96I GGNCC 5 cut(s) 4, 72, 178, 261, 262
ScaI AGTACT 1 cut(s) 773
SchI GAGTC 4 cut(s) 56, 65, 371, 527
ScrFI CCNGG 3 cut(s) 76, 112, 346
SduI GDGCHC 6 cut(s) 213, 225, 237, 265, 392, 470
SfaNI GCATC 4 cut(s) 132, 427, 449, 655
SfcI CTRYAG 1 cut(s) 750
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 2 cut(s) 4, 178
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 5 cut(s) 206, 398, 642, 692, 767
SmoI CTYRAG 5 cut(s) 206, 398, 642, 692, 767
Sse9I AATT 4 cut(s) 541, 558, 652, 865
SseBI AGGCCT 1 cut(s) 735
SspMI CTAG 2 cut(s) 587, 879
StuI AGGCCT 1 cut(s) 735
StyD4I CCNGG 3 cut(s) 74, 110, 344
StyI CCWWGG 4 cut(s) 444, 717, 736, 878
TaqI TCGA 3 cut(s) 207, 285, 410
TaqII GACCGA 2 cut(s) 21, 65
TasI AATT 4 cut(s) 541, 558, 652, 865
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 4 cut(s) 287, 337, 688, 954
Tru1I TTAA 3 cut(s) 477, 564, 855
Tru9I TTAA 3 cut(s) 477, 564, 855
TscAI CASTG 2 cut(s) 51, 260
TseFI GTSAC 2 cut(s) 46, 430
TseI GCWGC 2 cut(s) 163, 420
Tsp45I GTSAC 2 cut(s) 46, 430
TspDTI ATGAA 1 cut(s) 519
TspGWI ACGGA 2 cut(s) 583, 911
TspRI CASTG 2 cut(s) 51, 260
VneI GTGCAC 1 cut(s) 466
VpaK11BI GGWCC 2 cut(s) 4, 178
XapI RAATTY 2 cut(s) 652, 865
XhoI CTCGAG 1 cut(s) 206
XmaJI CCTAGG 1 cut(s) 878
XspI CTAG 2 cut(s) 587, 879
ZrmI AGTACT 1 cut(s) 773
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.