Rh2BG010300

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002120
Physical Location & Seq
Reverse (-)
740159 .. 742077
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG010300.1

Sequence Viewer

Length: 933 bp
ATGGACCGAACCAATAATGAAGAAGAACCACCAGTGACCGACAAGTCCGACTCCGACTGGGCCGGGTTGACCTACGAGTGCCTCGCCAACATCCTCGCCCGGCTTCCCTTGGAGCACAGATGGCGTGCGATGCTCCTCTGCAAATCATGGCTGCTCGCCTGCAAGGACCCGCATCTCAACTCGCGCTTCGTCCTCGAGCCCAAGCCCGAGTACGGGTCGGCTCAGTGGTGGGCCCCCGAGTTGGGGAGAAGGGTCGATTCCATTCTCCGATCTGTTGCTGAATGGAGCGAGGGGTCGCTTAGGGAGATTCGCACCCGGAACTGCTCTAATGACTCTCTCCATTTCGTCGCCCAAAGGTGCCCAAACCTTGAGGTTCTTTCGATCAAGTGCTGCCCGAATGTGACTGATGCATCCATGGCTGACATAGCGTTTCGGTGCACCATGATTAAGGAACTTGACATCAGCTATTGCCATGAAATATCTCACGAGTCTCTGGTGTTGATTGGGAAGAATTGCCCTAATCTCACAATTGTTAAACGGAATATGTTGAATTTGCTAGATCCTTCTGAACACGCTGGGATTGTTCCTGAGGAGTATTTAAAAACTTGCCCTCAAGATGGAAATTCAGAAGCTGATGCAATTGGCAAATCTATGCCAAATCTCAAGCACCTTGAACTAAGGTTCTCCAAGGTAACGGCTAAAGGCCTTGGTTTGATATCTGCAGGGTGTCTGAAACTTGAGTACTTGGATGTGTTTGGGTGTGTAAACTTGAATAGTCGTGATATCAACAATGCAACATCCGAGCTGAAGAACTTGAAGGAGATTAAAAAGCCAAATTTCTACATCCCTAGGTCGGTTTTCCATACAGAGGAGAGGTATGGACATTGGAGGCTGTATGATGAGAGATTTCAAACAGATATTTTCAGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0019005 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LRR_6

Protein Analysis

310

Amino Acids

35.5

Weight (kDa)

6.17

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 60 1.7e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 357
AccII CGCG 1 cut(s) 184
AciI CCGC 1 cut(s) 170
AclWI GGATC 1 cut(s) 554
AcsI RAATTY 3 cut(s) 550, 622, 835
AcuI CTGAAG 1 cut(s) 827
AfaI GTAC 2 cut(s) 212, 743
AfiI CCNNNNNNNGG 8 cut(s) 212, 213, 241, 242, 243, 617, 853, 868
AgsI TTSAA 5 cut(s) 550, 674, 772, 817, 911
AluBI AGCT 3 cut(s) 465, 632, 805
AluI AGCT 3 cut(s) 465, 632, 805
Alw21I GWGCWC 2 cut(s) 117, 440
Alw26I GTCTC 1 cut(s) 495
Alw44I GTGCAC 1 cut(s) 436
AlwI GGATC 1 cut(s) 554
AlwNI CAGNNNCTG 1 cut(s) 632
Ama87I CYCGRG 3 cut(s) 194, 206, 236
AoxI GGCC 3 cut(s) 60, 231, 703
ApaI GGGCCC 1 cut(s) 235
ApaLI GTGCAC 1 cut(s) 436
ApeKI GCWGC 2 cut(s) 151, 390
ApoI RAATTY 3 cut(s) 550, 622, 835
AspA2I CCTAGG 1 cut(s) 848
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 5 cut(s) 4, 60, 166, 231, 232
AsuC2I CCSGG 3 cut(s) 64, 100, 316
AvaI CYCGRG 3 cut(s) 194, 206, 236
AvaII GGWCC 2 cut(s) 4, 166
AvrII CCTAGG 1 cut(s) 848
AxyI CCTNAGG 1 cut(s) 588
BaeGI GKGCMC 3 cut(s) 235, 362, 440
BanI GGYRCC 1 cut(s) 357
BanII GRGCYC 2 cut(s) 201, 235
BauI CACGAG 1 cut(s) 485
Bbv12I GWGCWC 2 cut(s) 117, 440
BbvI GCAGC 2 cut(s) 138, 377
BccI CCATC 2 cut(s) 114, 611
BceAI ACGGC 1 cut(s) 711
BcnI CCSGG 3 cut(s) 64, 100, 316
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 2 cut(s) 557, 849
BfmI CTRYAG 1 cut(s) 720
BisI GCNGC 2 cut(s) 152, 391
BlnI CCTAGG 1 cut(s) 848
BlsI GCNGC 2 cut(s) 153, 392
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 3 cut(s) 64, 100, 316
Bme18I GGWCC 2 cut(s) 4, 166
BmeT110I CYCGRG 3 cut(s) 194, 206, 236
BmgT120I GGNCC 5 cut(s) 4, 60, 166, 231, 232
BmiI GGNNCC 4 cut(s) 168, 233, 234, 359
BmrFI CCNGG 3 cut(s) 64, 100, 316
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 5 cut(s) 120, 181, 397, 419, 625
BmuI ACTGGG 1 cut(s) 67
Bpu10I CCTNAGC 1 cut(s) 299
BpuEI CTTGAG 4 cut(s) 389, 597, 647, 758
BpuMI CCSGG 3 cut(s) 64, 100, 316
BsaJI CCNNGG 5 cut(s) 108, 414, 687, 706, 848
Bsc4I CCNNNNNNNGG 8 cut(s) 212, 213, 241, 242, 243, 617, 853, 868
Bse1I ACTGG 2 cut(s) 32, 62
Bse21I CCTNAGG 1 cut(s) 588
BseDI CCNNGG 5 cut(s) 108, 414, 687, 706, 848
BseGI GGATG 5 cut(s) 90, 410, 754, 797, 843
BseLI CCNNNNNNNGG 8 cut(s) 212, 213, 241, 242, 243, 617, 853, 868
BseMII CTCAG 2 cut(s) 236, 579
BseNI ACTGG 2 cut(s) 32, 62
BseRI GAGGAG 3 cut(s) 125, 605, 884
BseSI GKGCMC 3 cut(s) 235, 362, 440
BseXI GCAGC 2 cut(s) 138, 377
BseYI CCCAGC 1 cut(s) 575
Bsh1236I CGCG 1 cut(s) 184
BshFI GGCC 3 cut(s) 62, 233, 705
BshNI GGYRCC 1 cut(s) 357
BsiHKAI GWGCWC 2 cut(s) 117, 440
BsiHKCI CYCGRG 3 cut(s) 194, 206, 236
BsiSI CCGG 3 cut(s) 63, 100, 316
BslI CCNNNNNNNGG 8 cut(s) 212, 213, 241, 242, 243, 617, 853, 868
BsmAI GTCTC 1 cut(s) 495
BsnI GGCC 3 cut(s) 62, 233, 705
BsoBI CYCGRG 3 cut(s) 194, 206, 236
Bsp120I GGGCCC 1 cut(s) 231
Bsp1286I GDGCHC 5 cut(s) 117, 201, 235, 362, 440
Bsp143I GATC 3 cut(s) 269, 381, 559
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 1 cut(s) 170
BspANI GGCC 3 cut(s) 62, 233, 705
BspCNI CTCAG 2 cut(s) 235, 580
BspFNI CGCG 1 cut(s) 184
BspLI GGNNCC 4 cut(s) 168, 233, 234, 359
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 357
BsrI ACTGG 2 cut(s) 32, 62
BssECI CCNNGG 5 cut(s) 108, 414, 687, 706, 848
BssMI GATC 3 cut(s) 269, 381, 559
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 5 cut(s) 108, 414, 687, 706, 848
Bst2BI CACGAG 1 cut(s) 485
BstC8I GCNNGC 3 cut(s) 126, 156, 160
BstDEI CTNAG 4 cut(s) 222, 299, 588, 677
BstDSI CCRYGG 1 cut(s) 414
BstF5I GGATG 5 cut(s) 90, 410, 754, 797, 843
BstFNI CGCG 1 cut(s) 184
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 3 cut(s) 272, 384, 562
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 3 cut(s) 269, 381, 559
BstMWI GCNNNNNNNGC 4 cut(s) 121, 130, 416, 425
BstSCI CCNGG 3 cut(s) 62, 98, 314
BstSFI CTRYAG 1 cut(s) 720
BstSLI GKGCMC 3 cut(s) 235, 362, 440
BstUI CGCG 1 cut(s) 184
BstV1I GCAGC 2 cut(s) 138, 377
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
Bsu36I CCTNAGG 1 cut(s) 588
BsuRI GGCC 3 cut(s) 62, 233, 705
BtgI CCRYGG 1 cut(s) 414
BtgZI GCGATG 1 cut(s) 143
BtsCI GGATG 5 cut(s) 90, 410, 754, 797, 843
BtsIMutI CAGTG 2 cut(s) 39, 230
Cac8I GCNNGC 3 cut(s) 126, 156, 160
CaiI CAGNNNCTG 1 cut(s) 632
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 5 cut(s) 4, 60, 166, 231, 232
Csp6I GTAC 2 cut(s) 211, 742
CviAII CATG 4 cut(s) 147, 415, 442, 473
CviQI GTAC 2 cut(s) 211, 742
DdeI CTNAG 4 cut(s) 222, 299, 588, 677
DpnI GATC 3 cut(s) 271, 383, 561
DpnII GATC 3 cut(s) 269, 381, 559
DraI TTTAAA 1 cut(s) 600
DrdI GACNNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 1 cut(s) 43
Eco130I CCWWGG 5 cut(s) 108, 414, 687, 706, 848
Eco147I AGGCCT 1 cut(s) 705
Eco24I GRGCYC 2 cut(s) 201, 235
Eco32I GATATC 2 cut(s) 717, 784
Eco47I GGWCC 2 cut(s) 4, 166
Eco57I CTGAAG 1 cut(s) 827
Eco81I CCTNAGG 1 cut(s) 588
Eco88I CYCGRG 3 cut(s) 194, 206, 236
EcoO109I RGGNCCY 2 cut(s) 166, 232
EcoRV GATATC 2 cut(s) 717, 784
EcoT14I CCWWGG 5 cut(s) 108, 414, 687, 706, 848
EcoT22I ATGCAT 1 cut(s) 412
EcoT38I GRGCYC 2 cut(s) 201, 235
ErhI CCWWGG 5 cut(s) 108, 414, 687, 706, 848
FaeI CATG 4 cut(s) 150, 418, 445, 476
FatI CATG 4 cut(s) 146, 414, 441, 472
FauI CCCGC 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 152, 391
FokI GGATG 5 cut(s) 77, 397, 761, 784, 830
FriOI GRGCYC 2 cut(s) 201, 235
Fsp4HI GCNGC 2 cut(s) 152, 391
FspBI CTAG 2 cut(s) 557, 849
GlaI GCGC 1 cut(s) 185
GluI GCNGC 2 cut(s) 152, 391
GsaI CCCAGC 1 cut(s) 579
HaeIII GGCC 3 cut(s) 62, 233, 705
HapII CCGG 3 cut(s) 63, 100, 316
HhaI GCGC 1 cut(s) 186
Hin1II CATG 4 cut(s) 150, 418, 445, 476
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 6 cut(s) 50, 257, 307, 332, 488, 927
HpaII CCGG 3 cut(s) 63, 100, 316
Hpy166II GTNNAC 3 cut(s) 69, 438, 766
Hpy188I TCNGA 8 cut(s) 49, 55, 269, 568, 628, 732, 802, 926
Hpy188III TCNNGA 4 cut(s) 485, 587, 614, 779
Hpy8I GTNNAC 3 cut(s) 69, 438, 766
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 3 cut(s) 243, 573, 811
HpyCH4V TGCA 7 cut(s) 141, 162, 410, 438, 638, 722, 794
HpyF10VI GCNNNNNNNGC 4 cut(s) 121, 130, 416, 425
HpyF3I CTNAG 4 cut(s) 222, 299, 588, 677
Hsp92II CATG 4 cut(s) 150, 418, 445, 476
HspAI GCGC 1 cut(s) 184
Kzo9I GATC 3 cut(s) 269, 381, 559
LmnI GCTCC 3 cut(s) 112, 138, 285
Lsp1109I GCAGC 2 cut(s) 138, 377
LweI GCATC 5 cut(s) 120, 181, 397, 419, 625
MaeI CTAG 2 cut(s) 557, 849
MaeIII GTNAC 3 cut(s) 34, 400, 691
MalI GATC 3 cut(s) 271, 383, 561
MboI GATC 3 cut(s) 269, 381, 559
MboII GAAGA 4 cut(s) 32, 35, 520, 820
MfeI CAATTG 2 cut(s) 528, 639
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 5 cut(s) 117, 201, 235, 362, 440
MluCI AATT 6 cut(s) 511, 528, 550, 622, 639, 835
MlyI GAGTC 3 cut(s) 44, 326, 497
MmeI TCCRAC 2 cut(s) 72, 78
Mph1103I ATGCAT 1 cut(s) 412
MseI TTAA 4 cut(s) 447, 534, 599, 825
MspI CCGG 3 cut(s) 63, 100, 316
MspR9I CCNGG 3 cut(s) 64, 100, 316
MunI CAATTG 2 cut(s) 528, 639
MvnI CGCG 1 cut(s) 184
MwoI GCNNNNNNNGC 4 cut(s) 121, 130, 416, 425
NciI CCSGG 3 cut(s) 64, 100, 316
NcoI CCATGG 1 cut(s) 414
NdeII GATC 3 cut(s) 269, 381, 559
NlaIII CATG 4 cut(s) 150, 418, 445, 476
NlaIV GGNNCC 4 cut(s) 168, 233, 234, 359
NmuCI GTSAC 2 cut(s) 34, 400
NsiI ATGCAT 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 194
PceI AGGCCT 1 cut(s) 705
PfeI GAWTC 3 cut(s) 257, 307, 927
PkrI GCNGC 2 cut(s) 153, 392
PleI GAGTC 3 cut(s) 44, 326, 496
PpsI GAGTC 3 cut(s) 44, 326, 496
PpuMI RGGWCCY 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 575
PspN4I GGNNCC 4 cut(s) 168, 233, 234, 359
PspOMI GGGCCC 1 cut(s) 231
PspPI GGNCC 5 cut(s) 4, 60, 166, 231, 232
PspPPI RGGWCCY 1 cut(s) 166
PspXI VCTCGAGB 1 cut(s) 194
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 2 cut(s) 212, 743
RsaNI GTAC 2 cut(s) 211, 742
SaqAI TTAA 4 cut(s) 447, 534, 599, 825
SatI GCNGC 2 cut(s) 152, 391
Sau3AI GATC 3 cut(s) 269, 381, 559
Sau96I GGNCC 5 cut(s) 4, 60, 166, 231, 232
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 3 cut(s) 44, 326, 497
ScrFI CCNGG 3 cut(s) 64, 100, 316
SduI GDGCHC 5 cut(s) 117, 201, 235, 362, 440
SfaNI GCATC 5 cut(s) 120, 181, 397, 419, 625
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 2 cut(s) 4, 166
SlaI CTCGAG 1 cut(s) 194
SmlI CTYRAG 5 cut(s) 194, 368, 612, 662, 737
SmoI CTYRAG 5 cut(s) 194, 368, 612, 662, 737
Sse9I AATT 6 cut(s) 511, 528, 550, 622, 639, 835
SseBI AGGCCT 1 cut(s) 705
SsiI CCGC 1 cut(s) 170
SspMI CTAG 2 cut(s) 557, 849
StuI AGGCCT 1 cut(s) 705
StyD4I CCNGG 3 cut(s) 62, 98, 314
StyI CCWWGG 5 cut(s) 108, 414, 687, 706, 848
TaqI TCGA 3 cut(s) 195, 255, 380
TaqII GACCGA 2 cut(s) 21, 53
TasI AATT 6 cut(s) 511, 528, 550, 622, 639, 835
TatI WGTACW 1 cut(s) 741
TfiI GAWTC 3 cut(s) 257, 307, 927
Tru1I TTAA 4 cut(s) 447, 534, 599, 825
Tru9I TTAA 4 cut(s) 447, 534, 599, 825
TscAI CASTG 2 cut(s) 39, 230
TseFI GTSAC 2 cut(s) 34, 400
TseI GCWGC 2 cut(s) 151, 390
Tsp45I GTSAC 2 cut(s) 34, 400
TspDTI ATGAA 2 cut(s) 33, 489
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 2 cut(s) 39, 230
VneI GTGCAC 1 cut(s) 436
VpaK11BI GGWCC 2 cut(s) 4, 166
XapI RAATTY 3 cut(s) 550, 622, 835
XhoI CTCGAG 1 cut(s) 194
XmaJI CCTAGG 1 cut(s) 848
XspI CTAG 2 cut(s) 557, 849
ZrmI AGTACT 1 cut(s) 743
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.