FvH4_3g10650

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000812
Physical Location & Seq
Reverse (-)
6343393 .. 6354871
11479 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10650.t2

Sequence Viewer

Length: 765 bp
ATGGTCTCTCCCTATAAATACACAGAAACATGCTGGTCTCATTCCACAATCAAGTTTCCTTGCTTTCCAAGCTTAAACAAAGTATTTCCAAACAAAGAAACAAAATCCAAAGTTCCAATGGCCTCGCAGTTTCTTTTGTTAGCATGCCTTGCTATAACAGCTTCCATTGCTTTAGCATCCGACCCAAGTTCCCTTCAGGATTTCTGTGTGGCTGATCCATCAACCTCAGTACCAGTGAATGGACATGTATGCAAGGACCCCAAGCTTGTTGTTGCAGATGACTTCTTCTTCAGTGGACTTCACTTAGCAGGCAACACATCCAACCCTGCTGGTTCAAGGGTGACCCCTGCTAATGTAGCCCAAATTCCAGGACTCAATACTTTTGGCATCTCCCTTGTTCGTATCGACTATGCACCATGGGGTATCAATGCTCCACACACTCATCCTAGAGCGACTGAAATCTTGACAGTCCTTGAAGGGAGCCTCCAAGTGGGTTTTGTCACCTCCAACCCCGAAAACAGACTTATCACAAAGGTGCTTCAGAAGGGTGATGTGTTTGTATTCCCAGTGGGACTTGTGCATTTCCAGCACAACGTTGGAAAGGTTAATGCAGTCGCCATTGCAGCTCTGAGCAGCCAAAATCCAGGTGTTATTAGCATTGCTAATGTAGTCTTTGGATCAAAGCCTGCCATTTCTGCTGATGTTCTTGCAAAGGCATTCCAGGTTGACAAGGACACAATCTATGATTTCCAGTCCAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

255

Amino Acids

27.19

Weight (kDa)

8.25

Isoelectric Point (pI)

32.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 62 - 208 9e-53 Cupin
Cupin_2 PF07883 97 - 169 5.4e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 239
AclI AACGTT 1 cut(s) 594
AclWI GGATC 2 cut(s) 209, 685
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 3 cut(s) 179, 274, 524
AfaI GTAC 1 cut(s) 231
AfiI CCNNNNNNNGG 2 cut(s) 239, 490
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 2 cut(s) 336, 476
AjnI CCWGG 3 cut(s) 367, 643, 720
AleI CACNNNNGTG 1 cut(s) 533
AluBI AGCT 4 cut(s) 72, 161, 265, 626
AluI AGCT 4 cut(s) 72, 161, 265, 626
Alw26I GTCTC 2 cut(s) 10, 42
AlwI GGATC 2 cut(s) 209, 685
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 2 cut(s) 623, 633
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 256
AsuHPI GGTGA 3 cut(s) 352, 493, 560
AvaII GGWCC 1 cut(s) 256
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BbvI GCAGC 2 cut(s) 635, 645
BccI CCATC 1 cut(s) 226
BciT130I CCWGG 3 cut(s) 369, 645, 722
BcoDI GTCTC 2 cut(s) 10, 42
BfaI CTAG 2 cut(s) 447, 763
BisI GCNGC 2 cut(s) 624, 634
BlsI GCNGC 2 cut(s) 625, 635
Bme1390I CCNGG 3 cut(s) 369, 645, 722
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 2 cut(s) 258, 482
BmrFI CCNGG 3 cut(s) 369, 645, 722
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 2 cut(s) 185, 396
BmuI ACTGGG 1 cut(s) 560
BsaI GGTCTC 2 cut(s) 10, 42
BsaJI CCNNGG 1 cut(s) 416
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 490
Bse1I ACTGG 3 cut(s) 233, 566, 751
Bse3DI GCAATG 3 cut(s) 165, 618, 657
BseBI CCWGG 3 cut(s) 369, 645, 722
BseDI CCNNGG 1 cut(s) 416
BseGI GGATG 3 cut(s) 176, 317, 442
BseLI CCNNNNNNNGG 2 cut(s) 239, 490
BseMI GCAATG 3 cut(s) 165, 618, 657
BseMII CTCAG 2 cut(s) 240, 620
BseNI ACTGG 3 cut(s) 233, 566, 751
BseXI GCAGC 2 cut(s) 635, 645
BshFI GGCC 1 cut(s) 122
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 2 cut(s) 239, 490
BsmAI GTCTC 2 cut(s) 10, 42
BsmFI GGGAC 1 cut(s) 585
BsmI GAATGC 1 cut(s) 716
BsnI GGCC 1 cut(s) 122
Bso31I GGTCTC 2 cut(s) 10, 42
Bsp143I GATC 2 cut(s) 214, 677
Bsp19I CCATGG 1 cut(s) 416
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 2 cut(s) 239, 621
BspLI GGNNCC 2 cut(s) 258, 482
BspPI GGATC 2 cut(s) 209, 685
BspTNI GGTCTC 2 cut(s) 10, 42
BsrDI GCAATG 3 cut(s) 165, 618, 657
BsrI ACTGG 3 cut(s) 233, 566, 751
BssECI CCNNGG 1 cut(s) 416
BssMI GATC 2 cut(s) 214, 677
BssT1I CCWWGG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 369, 645, 722
Bst4CI ACNGT 1 cut(s) 469
BstAPI GCANNNNNTGC 1 cut(s) 149
BstC8I GCNNGC 3 cut(s) 145, 310, 687
BstDEI CTNAG 3 cut(s) 226, 304, 629
BstDSI CCRYGG 1 cut(s) 416
BstEII GGTNACC 1 cut(s) 340
BstF5I GGATG 3 cut(s) 176, 317, 442
BstKTI GATC 2 cut(s) 217, 680
BstMAI GTCTC 2 cut(s) 10, 42
BstMBI GATC 2 cut(s) 214, 677
BstMWI GCNNNNNNNGC 8 cut(s) 69, 149, 158, 167, 356, 586, 623, 695
BstNI CCWGG 3 cut(s) 369, 645, 722
BstNSI RCATGY 3 cut(s) 33, 147, 248
BstPI GGTNACC 1 cut(s) 340
BstSCI CCNGG 3 cut(s) 367, 643, 720
BstV1I GCAGC 2 cut(s) 635, 645
BsuRI GGCC 1 cut(s) 122
BtgI CCRYGG 1 cut(s) 416
BtsCI GGATG 3 cut(s) 176, 317, 442
BtsIMutI CAGTG 3 cut(s) 240, 298, 573
Cac8I GCNNGC 3 cut(s) 145, 310, 687
Cfr13I GGNCC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 230
CviAII CATG 4 cut(s) 30, 144, 245, 417
CviQI GTAC 1 cut(s) 230
DdeI CTNAG 3 cut(s) 226, 304, 629
DpnI GATC 2 cut(s) 216, 679
DpnII GATC 2 cut(s) 214, 677
Eco130I CCWWGG 1 cut(s) 416
Eco31I GGTCTC 2 cut(s) 10, 42
Eco47I GGWCC 1 cut(s) 256
Eco57I CTGAAG 3 cut(s) 179, 274, 524
Eco91I GGTNACC 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 256
EcoO65I GGTNACC 1 cut(s) 340
EcoRII CCWGG 3 cut(s) 367, 643, 720
EcoT14I CCWWGG 1 cut(s) 416
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 4 cut(s) 33, 147, 248, 420
FaiI YATR 9 cut(s) 15, 31, 145, 155, 246, 250, 411, 418, 744
FaqI GGGAC 1 cut(s) 585
FatI CATG 4 cut(s) 29, 143, 244, 416
Fnu4HI GCNGC 2 cut(s) 624, 634
FokI GGATG 3 cut(s) 163, 304, 429
Fsp4HI GCNGC 2 cut(s) 624, 634
FspBI CTAG 2 cut(s) 447, 763
GluI GCNGC 2 cut(s) 624, 634
HaeIII GGCC 1 cut(s) 122
Hin1II CATG 4 cut(s) 33, 147, 248, 420
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 2 cut(s) 70, 263
HinfI GANTC 1 cut(s) 372
HphI GGTGA 3 cut(s) 352, 493, 560
Hpy166II GTNNAC 2 cut(s) 296, 727
Hpy188I TCNGA 3 cut(s) 181, 543, 630
Hpy188III TCNNGA 2 cut(s) 197, 463
Hpy8I GTNNAC 2 cut(s) 296, 727
HpyAV CCTTC 3 cut(s) 203, 470, 538
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 7 cut(s) 252, 275, 413, 580, 611, 623, 710
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 149, 158, 167, 356, 586, 623, 695
HpyF3I CTNAG 3 cut(s) 226, 304, 629
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 4 cut(s) 33, 147, 248, 420
Kzo9I GATC 2 cut(s) 214, 677
LmnI GCTCC 2 cut(s) 436, 480
Lsp1109I GCAGC 2 cut(s) 635, 645
LweI GCATC 2 cut(s) 185, 396
MaeI CTAG 2 cut(s) 447, 763
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 2 cut(s) 340, 499
MalI GATC 2 cut(s) 216, 679
MboI GATC 2 cut(s) 214, 677
MboII GAAGA 2 cut(s) 277, 280
MluCI AATT 1 cut(s) 363
MlyI GAGTC 1 cut(s) 366
MmeI TCCRAC 4 cut(s) 204, 345, 531, 577
MnlI CCTC 4 cut(s) 133, 235, 494, 514
MseI TTAA 2 cut(s) 74, 606
MslI CAYNNNNRTG 1 cut(s) 533
MspR9I CCNGG 3 cut(s) 369, 645, 722
Mva1269I GAATGC 1 cut(s) 716
MvaI CCWGG 3 cut(s) 369, 645, 722
MwoI GCNNNNNNNGC 8 cut(s) 69, 149, 158, 167, 356, 586, 623, 695
NcoI CCATGG 1 cut(s) 416
NdeII GATC 2 cut(s) 214, 677
NlaIII CATG 4 cut(s) 33, 147, 248, 420
NlaIV GGNNCC 2 cut(s) 258, 482
NmuCI GTSAC 2 cut(s) 340, 499
NspI RCATGY 3 cut(s) 33, 147, 248
OliI CACNNNNGTG 1 cut(s) 533
PaeI GCATGC 1 cut(s) 147
PciI ACATGT 1 cut(s) 244
PctI GAATGC 1 cut(s) 716
PflMI CCANNNNNTGG 1 cut(s) 239
PfoI TCCNGGA 1 cut(s) 367
PkrI GCNGC 2 cut(s) 625, 635
PleI GAGTC 1 cut(s) 366
PpsI GAGTC 1 cut(s) 366
PpuMI RGGWCCY 1 cut(s) 256
PscI ACATGT 1 cut(s) 244
Psp1406I AACGTT 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 3 cut(s) 367, 643, 720
PspEI GGTNACC 1 cut(s) 340
PspGI CCWGG 3 cut(s) 367, 643, 720
PspN4I GGNNCC 2 cut(s) 258, 482
PspPI GGNCC 1 cut(s) 256
PspPPI RGGWCCY 1 cut(s) 256
RsaI GTAC 1 cut(s) 231
RsaNI GTAC 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 533
SaqAI TTAA 2 cut(s) 74, 606
SatI GCNGC 2 cut(s) 624, 634
Sau3AI GATC 2 cut(s) 214, 677
Sau96I GGNCC 1 cut(s) 256
SchI GAGTC 1 cut(s) 366
ScrFI CCNGG 3 cut(s) 369, 645, 722
SfaNI GCATC 2 cut(s) 185, 396
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 533
SphI GCATGC 1 cut(s) 147
Sse9I AATT 1 cut(s) 363
SspMI CTAG 2 cut(s) 447, 763
StyD4I CCNGG 3 cut(s) 367, 643, 720
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 1 cut(s) 597
TaqI TCGA 1 cut(s) 405
TasI AATT 1 cut(s) 363
Tru1I TTAA 2 cut(s) 74, 606
Tru9I TTAA 2 cut(s) 74, 606
TscAI CASTG 3 cut(s) 240, 298, 573
TseFI GTSAC 2 cut(s) 340, 499
TseI GCWGC 2 cut(s) 623, 633
Tsp45I GTSAC 2 cut(s) 340, 499
TspRI CASTG 3 cut(s) 240, 298, 573
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 1 cut(s) 363
XceI RCATGY 3 cut(s) 33, 147, 248
XcmI CCANNNNNNNNNTGG 2 cut(s) 115, 593
XspI CTAG 2 cut(s) 447, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.