RLG00000032280

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000812
Physical Location & Seq
Reverse (-)
13024521 .. 13025272
752 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032280

Sequence Viewer

Length: 648 bp
ATGGCCTCTCAGTTTCTATTGTTGGCATTCCTTGCCATAACTTGTTCCGTTGCTTTTGCTTCTGACCCAAGTTCTCTTCAGGACTTTTGTGTGGCTGAGCCTACAAGTTCAGTACCAGTGAATGGACATGTGTGCAAGGACCCCAAGCTTGTCGAAGCCAATGACTTCTTCCTCAGTGGACTGCACCTAGCAGGCAACACATCAAACCCTGCTGGTTCAAAGGTGACCCCTGCTAATGTAGCCCAGATTCCAGGACTTAACACTTTTGGTATCTCCCTAGTTCGTATAGACTATGCACCAGGGGGTATAAATTCTCCACACACTCATCCTAGAGCTACCGAAATCTTGACAGTGGTTGAAGGTAGCCTAGAAGTGGGTTTTGTCACCTCCAACCCTGAAAACCGTCTCATCTCCAAGGTGCTTCAGAAGGGTGATGTGTTTGTGTTCCCAGTAGGACTGGTGCATTTCCAGCACAATGTTGGAAAAGGTAATGCAGTCGCCATTGCAGCTCTCAGCAGCCAAAATCCAGGTGCTATTACCCTTGCCAACGCAGTGTTCGGATCAAAGCCAGACATCTCTGCCGATGTTCTTGCAAAGGCTTTCCAAGTGGATAAGAACATAGTCTTCGATTTTCAAGCCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

216

Amino Acids

22.52

Weight (kDa)

6.03

Isoelectric Point (pI)

24.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 61 - 207 5.2e-53 Cupin
Cupin_2 PF07883 96 - 168 6.3e-10 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 122
AclWI GGATC 1 cut(s) 568
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 2 cut(s) 62, 407
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 2 cut(s) 122, 373
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 3 cut(s) 219, 359, 635
AjnI CCWGG 3 cut(s) 250, 298, 526
AjuI GAANNNNNNNTTGG 4 cut(s) 152, 184, 539, 571
AluBI AGCT 3 cut(s) 148, 335, 509
AluI AGCT 3 cut(s) 148, 335, 509
Alw26I GTCTC 1 cut(s) 410
AlwI GGATC 1 cut(s) 568
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 506, 516
ApoI RAATTY 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 3 cut(s) 235, 376, 443
AvaII GGWCC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 616
BbvI GCAGC 2 cut(s) 518, 528
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 3 cut(s) 252, 300, 528
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 4 cut(s) 188, 278, 330, 368
BisI GCNGC 2 cut(s) 507, 517
BlpI GCTNAGC 1 cut(s) 96
BlsI GCNGC 2 cut(s) 508, 518
Bme1390I CCNGG 3 cut(s) 252, 300, 528
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 3 cut(s) 252, 300, 528
BmrI ACTGGG 1 cut(s) 443
BmuI ACTGGG 1 cut(s) 443
BpiI GAAGAC 1 cut(s) 616
Bpu1102I GCTNAGC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 299, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 373
Bse1I ACTGG 3 cut(s) 116, 449, 462
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 3 cut(s) 252, 300, 528
BseDI CCNNGG 2 cut(s) 299, 414
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 2 cut(s) 122, 373
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 4 cut(s) 23, 87, 187, 526
BseNI ACTGG 3 cut(s) 116, 449, 462
BseXI GCAGC 2 cut(s) 518, 528
BsgI GTGCAG 1 cut(s) 167
BshFI GGCC 1 cut(s) 5
BslI CCNNNNNNNGG 2 cut(s) 122, 373
BsmAI GTCTC 1 cut(s) 410
BsmBI CGTCTC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 560
Bsp1720I GCTNAGC 1 cut(s) 96
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 22, 88, 186, 525
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 1 cut(s) 568
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 3 cut(s) 116, 449, 462
BssECI CCNNGG 2 cut(s) 299, 414
BssMI GATC 1 cut(s) 560
BssT1I CCWWGG 1 cut(s) 414
Bst2UI CCWGG 3 cut(s) 252, 300, 528
Bst4CI ACNGT 2 cut(s) 352, 404
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 4 cut(s) 9, 96, 173, 512
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 1 cut(s) 325
BstKTI GATC 1 cut(s) 563
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 1 cut(s) 560
BstMWI GCNNNNNNNGC 4 cut(s) 32, 239, 469, 506
BstNI CCWGG 3 cut(s) 252, 300, 528
BstNSI RCATGY 1 cut(s) 131
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 3 cut(s) 250, 298, 526
BstV1I GCAGC 2 cut(s) 518, 528
BstV2I GAAGAC 1 cut(s) 616
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 325
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 4 cut(s) 123, 181, 357, 558
Cac8I GCNNGC 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 113
CviAII CATG 1 cut(s) 128
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 4 cut(s) 9, 96, 173, 512
DpnI GATC 1 cut(s) 562
DpnII GATC 1 cut(s) 560
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 62, 407
Eco91I GGTNACC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 139
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 3 cut(s) 250, 298, 526
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 410
FaeI CATG 1 cut(s) 131
FaiI YATR 6 cut(s) 38, 129, 287, 294, 308, 620
FatI CATG 1 cut(s) 127
Fnu4HI GCNGC 2 cut(s) 507, 517
FokI GGATG 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 507, 517
FspBI CTAG 4 cut(s) 188, 278, 330, 368
GluI GCNGC 2 cut(s) 507, 517
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 1 cut(s) 131
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 1 cut(s) 247
HphI GGTGA 3 cut(s) 235, 376, 443
Hpy166II GTNNAC 1 cut(s) 179
Hpy188I TCNGA 3 cut(s) 64, 426, 560
Hpy188III TCNNGA 2 cut(s) 80, 346
Hpy8I GTNNAC 1 cut(s) 179
HpyAV CCTTC 2 cut(s) 353, 421
HpyCH4III ACNGT 2 cut(s) 352, 404
HpyCH4V TGCA 7 cut(s) 135, 184, 296, 463, 494, 506, 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 239, 469, 506
HpyF3I CTNAG 4 cut(s) 9, 96, 173, 512
Hsp92II CATG 1 cut(s) 131
Kzo9I GATC 1 cut(s) 560
Lsp1109I GCAGC 2 cut(s) 518, 528
MaeI CTAG 4 cut(s) 188, 278, 330, 368
MaeIII GTNAC 2 cut(s) 223, 382
MalI GATC 1 cut(s) 562
MboI GATC 1 cut(s) 560
MboII GAAGA 3 cut(s) 68, 160, 616
MluCI AATT 1 cut(s) 310
MmeI TCCRAC 2 cut(s) 414, 460
MnlI CCTC 3 cut(s) 16, 182, 397
MseI TTAA 1 cut(s) 258
MspR9I CCNGG 3 cut(s) 252, 300, 528
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 3 cut(s) 252, 300, 528
MwoI GCNNNNNNNGC 4 cut(s) 32, 239, 469, 506
NdeII GATC 1 cut(s) 560
NlaIII CATG 1 cut(s) 131
NlaIV GGNNCC 1 cut(s) 141
NmuCI GTSAC 2 cut(s) 223, 382
NspI RCATGY 1 cut(s) 131
PciI ACATGT 1 cut(s) 127
PctI GAATGC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 247
PflMI CCANNNNNTGG 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 2 cut(s) 508, 518
PpuMI RGGWCCY 1 cut(s) 139
PscI ACATGT 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 250, 298, 526
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 3 cut(s) 250, 298, 526
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 139
PspPPI RGGWCCY 1 cut(s) 139
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 2 cut(s) 507, 517
Sau3AI GATC 1 cut(s) 560
Sau96I GGNCC 1 cut(s) 139
ScrFI CCNGG 3 cut(s) 252, 300, 528
SinI GGWCC 1 cut(s) 139
Sse9I AATT 1 cut(s) 310
SspMI CTAG 4 cut(s) 188, 278, 330, 368
StyD4I CCNGG 3 cut(s) 250, 298, 526
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 352, 404
TaqI TCGA 2 cut(s) 153, 627
TasI AATT 1 cut(s) 310
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 4 cut(s) 123, 181, 357, 558
TseFI GTSAC 2 cut(s) 223, 382
TseI GCWGC 2 cut(s) 506, 516
Tsp45I GTSAC 2 cut(s) 223, 382
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 4 cut(s) 123, 181, 357, 558
Van91I CCANNNNNTGG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 139
XapI RAATTY 1 cut(s) 310
XceI RCATGY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XspI CTAG 4 cut(s) 188, 278, 330, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.