Rh5BG127400

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000812
Physical Location & Seq
Forward (+)
12147830 .. 12148910
1081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG127400.1

Sequence Viewer

Length: 645 bp
ATGGCCTCTAAGTTTCTAATTTTGGCATTCCTTGCCATAACTTCTTCCCTTGCTTTTGCTGACCCAAGTTCCCTTCAGGATTTCTGTGTGGCTGATCTGAAAAGCTCAGTACCAGTTAATGGCCTTGTCTGCAAGGACCCCAAGCTTATTGAAGCCAATGACTTCTTCTTCAGTGGACTTCACCTAGTAGGCAACGCATCAAACCCTGCTGGTTCACGTGTGACCCCTGCGAACGTGGCCCAAATTCCAGGACTTAATACTCTTGGTATCTCCCTTGTTCGTATCGACTATGCACCATGGGGCATCAATGCTCCCCACACTCATCCTAGAGCCACTGAAGTCCTAACAGTCCTCGAAGGGACCCTCCAAGTGGGTTTTGTCACTTCCAACCCCGAAAACAGACTTATCACAAAGGTGCTTCAGAAAGGTGATGTGTTTGTTTTCCCAGTAGGACTTGTGCATTTCCAGCACAACGTTGGAAAGGTTAATGCAGTCGCCATTGCCGCTCTCAGCAGCCAAAATCCAGGTGTTATTAGCATCGCCAATTCAGTATTTGGATCTAAGCCTGCTATCTCTGCTGATGTTCTTGCAAAGGCTTTCCAAGTGGATAAGGACACAATCTACAATCTTCAGTCAAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

214

Amino Acids

22.64

Weight (kDa)

7.82

Isoelectric Point (pI)

25.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 64 - 206 6.4e-52 Cupin
Cupin_2 PF07883 95 - 167 2.7e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 506
AciI CCGC 1 cut(s) 504
AclI AACGTT 1 cut(s) 474
AclWI GGATC 1 cut(s) 565
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 5 cut(s) 59, 154, 357, 404, 614
AcvI CACGTG 1 cut(s) 218
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 2 cut(s) 119, 370
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 1 cut(s) 152
AjnI CCWGG 2 cut(s) 247, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AleI CACNNNNGTG 1 cut(s) 413
AluBI AGCT 2 cut(s) 105, 145
AluI AGCT 2 cut(s) 105, 145
AlwI GGATC 1 cut(s) 565
AoxI GGCC 3 cut(s) 3, 121, 237
ApeKI GCWGC 1 cut(s) 513
ApoI RAATTY 1 cut(s) 243
AspS9I GGNCC 3 cut(s) 136, 238, 360
AsuHPI GGTGA 2 cut(s) 173, 440
AvaII GGWCC 2 cut(s) 136, 360
BbrPI CACGTG 1 cut(s) 218
BbvI GCAGC 1 cut(s) 525
BciT130I CCWGG 2 cut(s) 249, 525
BfaI CTAG 3 cut(s) 185, 327, 643
BisI GCNGC 2 cut(s) 504, 514
BlsI GCNGC 2 cut(s) 505, 515
Bme1390I CCNGG 2 cut(s) 249, 525
Bme18I GGWCC 2 cut(s) 136, 360
BmgT120I GGNCC 3 cut(s) 136, 238, 360
BmiI GGNNCC 3 cut(s) 138, 361, 362
BmrFI CCNGG 2 cut(s) 249, 525
BmrI ACTGGG 1 cut(s) 440
BmsI GCATC 3 cut(s) 206, 312, 546
BmuI ACTGGG 1 cut(s) 440
BsaAI YACGTR 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 296
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 370
Bse1I ACTGG 2 cut(s) 113, 446
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 2 cut(s) 249, 525
BseDI CCNNGG 1 cut(s) 296
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 2 cut(s) 119, 370
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 2 cut(s) 120, 523
BseNI ACTGG 2 cut(s) 113, 446
BseXI GCAGC 1 cut(s) 525
BshFI GGCC 3 cut(s) 5, 123, 239
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 2 cut(s) 119, 370
BsmFI GGGAC 1 cut(s) 373
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 3 cut(s) 5, 123, 239
Bsp143I GATC 2 cut(s) 94, 557
Bsp19I CCATGG 1 cut(s) 296
BspACI CCGC 1 cut(s) 504
BspANI GGCC 3 cut(s) 5, 123, 239
BspCNI CTCAG 2 cut(s) 119, 522
BspLI GGNNCC 3 cut(s) 138, 361, 362
BspPI GGATC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 498
BsrI ACTGG 2 cut(s) 113, 446
BssECI CCNNGG 1 cut(s) 296
BssMI GATC 2 cut(s) 94, 557
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 2 cut(s) 249, 525
Bst4CI ACNGT 1 cut(s) 349
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 4 cut(s) 9, 106, 509, 561
BstDSI CCRYGG 1 cut(s) 296
BstF5I GGATG 1 cut(s) 322
BstKTI GATC 2 cut(s) 97, 560
BstMBI GATC 2 cut(s) 94, 557
BstMWI GCNNNNNNNGC 6 cut(s) 32, 129, 236, 466, 503, 575
BstNI CCWGG 2 cut(s) 249, 525
BstSCI CCNGG 2 cut(s) 247, 523
BstV1I GCAGC 1 cut(s) 525
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
BsuRI GGCC 3 cut(s) 5, 123, 239
BtgI CCRYGG 1 cut(s) 296
BtgZI GCGATG 1 cut(s) 523
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 2 cut(s) 178, 333
Cac8I GCNNGC 1 cut(s) 567
Cfr13I GGNCC 3 cut(s) 136, 238, 360
Csp6I GTAC 1 cut(s) 110
CviAII CATG 1 cut(s) 297
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 4 cut(s) 9, 106, 509, 561
DpnI GATC 2 cut(s) 96, 559
DpnII GATC 2 cut(s) 94, 557
Eco130I CCWWGG 1 cut(s) 296
Eco47I GGWCC 2 cut(s) 136, 360
Eco57I CTGAAG 5 cut(s) 59, 154, 357, 404, 614
Eco72I CACGTG 1 cut(s) 218
EcoO109I RGGNCCY 2 cut(s) 136, 360
EcoRII CCWGG 2 cut(s) 247, 523
EcoT14I CCWWGG 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 1 cut(s) 300
FaiI YATR 3 cut(s) 38, 291, 298
FaqI GGGAC 1 cut(s) 373
FatI CATG 1 cut(s) 296
Fnu4HI GCNGC 2 cut(s) 504, 514
FokI GGATG 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 504, 514
FspBI CTAG 3 cut(s) 185, 327, 643
GluI GCNGC 2 cut(s) 504, 514
HaeIII GGCC 3 cut(s) 5, 123, 239
Hin1II CATG 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 143
HphI GGTGA 2 cut(s) 173, 440
Hpy166II GTNNAC 2 cut(s) 176, 215
Hpy188I TCNGA 2 cut(s) 99, 423
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 2 cut(s) 176, 215
HpyAV CCTTC 2 cut(s) 83, 350
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 3 cut(s) 217, 234, 474
HpyCH4V TGCA 5 cut(s) 132, 293, 460, 491, 590
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 129, 236, 466, 503, 575
HpyF3I CTNAG 4 cut(s) 9, 106, 509, 561
HpySE526I ACGT 3 cut(s) 217, 234, 474
Hsp92II CATG 1 cut(s) 300
KflI GGGWCCC 1 cut(s) 360
Kzo9I GATC 2 cut(s) 94, 557
LmnI GCTCC 1 cut(s) 316
Lsp1109I GCAGC 1 cut(s) 525
LweI GCATC 3 cut(s) 206, 312, 546
MaeI CTAG 3 cut(s) 185, 327, 643
MaeII ACGT 3 cut(s) 217, 234, 474
MaeIII GTNAC 2 cut(s) 220, 379
MalI GATC 2 cut(s) 96, 559
MbiI CCGCTC 1 cut(s) 506
MboI GATC 2 cut(s) 94, 557
MboII GAAGA 4 cut(s) 36, 157, 160, 620
MflI RGATCY 1 cut(s) 557
MluCI AATT 3 cut(s) 18, 243, 544
MmeI TCCRAC 2 cut(s) 411, 457
MnlI CCTC 3 cut(s) 16, 362, 374
MseI TTAA 3 cut(s) 117, 255, 486
MslI CAYNNNNRTG 1 cut(s) 413
MspR9I CCNGG 2 cut(s) 249, 525
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 2 cut(s) 249, 525
MwoI GCNNNNNNNGC 6 cut(s) 32, 129, 236, 466, 503, 575
NcoI CCATGG 1 cut(s) 296
NdeII GATC 2 cut(s) 94, 557
NlaIII CATG 1 cut(s) 300
NlaIV GGNNCC 3 cut(s) 138, 361, 362
NmuCI GTSAC 2 cut(s) 220, 379
OliI CACNNNNGTG 1 cut(s) 413
PctI GAATGC 1 cut(s) 26
PflMI CCANNNNNTGG 1 cut(s) 119
PfoI TCCNGGA 1 cut(s) 247
PkrI GCNGC 2 cut(s) 505, 515
PmaCI CACGTG 1 cut(s) 218
PmlI CACGTG 1 cut(s) 218
Ppu21I YACGTR 1 cut(s) 218
PpuMI RGGWCCY 2 cut(s) 136, 360
Psp1406I AACGTT 1 cut(s) 474
Psp5II RGGWCCY 2 cut(s) 136, 360
Psp6I CCWGG 2 cut(s) 247, 523
PspCI CACGTG 1 cut(s) 218
PspGI CCWGG 2 cut(s) 247, 523
PspN4I GGNNCC 3 cut(s) 138, 361, 362
PspPI GGNCC 3 cut(s) 136, 238, 360
PspPPI RGGWCCY 2 cut(s) 136, 360
PsuI RGATCY 1 cut(s) 557
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 3 cut(s) 117, 255, 486
SatI GCNGC 2 cut(s) 504, 514
Sau3AI GATC 2 cut(s) 94, 557
Sau96I GGNCC 3 cut(s) 136, 238, 360
ScrFI CCNGG 2 cut(s) 249, 525
SfaNI GCATC 3 cut(s) 206, 312, 546
SinI GGWCC 2 cut(s) 136, 360
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 3 cut(s) 18, 243, 544
SsiI CCGC 1 cut(s) 504
SspMI CTAG 3 cut(s) 185, 327, 643
StyD4I CCNGG 2 cut(s) 247, 523
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 3 cut(s) 220, 237, 477
TaqI TCGA 2 cut(s) 285, 354
TasI AATT 3 cut(s) 18, 243, 544
TauI GCSGC 1 cut(s) 506
Tru1I TTAA 3 cut(s) 117, 255, 486
Tru9I TTAA 3 cut(s) 117, 255, 486
TscAI CASTG 2 cut(s) 178, 340
TseFI GTSAC 2 cut(s) 220, 379
TseI GCWGC 1 cut(s) 513
Tsp45I GTSAC 2 cut(s) 220, 379
TspRI CASTG 2 cut(s) 178, 340
Van91I CCANNNNNTGG 1 cut(s) 119
VpaK11BI GGWCC 2 cut(s) 136, 360
XapI RAATTY 1 cut(s) 243
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XspI CTAG 3 cut(s) 185, 327, 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.