RchiOBHm_Chr5g0017351

germin-like protein 2-3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000812
Physical Location & Seq
Reverse (-)
12024258 .. 12026901
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29765

Sequence Viewer

Length: 1398 bp
ATGGCCTCGCAGTTTCTATTGTTAGCATGCCTTGCTATGACAGCTTCTATTGCTTTGGCATCTGACCCAAGTCCTCTTCAGGATTTTTGTGTGGCTGATCCATCAGGCACAGTACCCGTGAATGGACATGTGTGCAAGGACCCCAAGCTAGTTGTCGCCGATGACTTCTTCTTCAGTGGACTTCACCTTGCAGGCAACACATCAAACCCTGCTGGTTCAAAGGTGACCCCTGCTAATGTTGCCCAGATTCCAGGACTTAATACTTTTGGTATCTCCCTAGTTCGTATCGACTATGCACCATGGGGTATCAATGCTCCACACACTCATCCTAGAGCTACTGAAATCTTGACAGTCCTTGAAGGGAGCCTCCAAGTGGGTTTTGTCACCTCCAATCCCGAAAACAGACTTATCACAAAGGTGCTTCAGAAGGGCGATGTGTTTGTGTTCCCAGTAGGACTAGTGCATTTCCAACACAATGTGGGAAATGTTAATGCGGTAGCCATTGCAGCTCTGAGCAGCCAAAATCCAGGTGCTATTACCATTGCCAATGCAGTGTTTGGATCAAAGCCTGCCATTTCTGCTGATGTTCTTGCAAAGGCATTCCAAGTCGACAAGGACACAATCTACGATTTCCAGTCCAAGATCATATGGTGGTCCCAAACCACAATTCGACTGATCAAGTTTGTTCTTGCTTACAAAGTTAAGCAAATTTTGTCCATCTGTCTGTCTAGTGAGAATCAATCAATTCCAAAAATGGCCTCTCGATTTCTGTTGCTAGCATTACTCGTAGTCATGACTTGCTCCATTGCTTTGGCAACTGAGAGTCTTCAAGATTTCTGCGTAGCACAGCCAACACGCTTAGCTGCAGTGAATGGCGTTGTCTGCAAGGACCCTAATCTTGTTGAAGCCAATGACTTCTACTATAGTGGACTTGACATAGCAGGCAACGCATCAACTGCATTCGGTTCACGTGTGACCTCTGTTAGGGTGAACCAAATTCCAGGACTCAACACCTTTGGCATCTCCATTGCACGCATCGATTTTGCTGCATGGGGTGTTAACCCTCCCCACACACACCCTCGCGCTGCCGAAATCTTGACAGTCCTTGAAGGCAGCCTCCAAGTTGGTTTTGTCACCTCCAACCCCGATAACCGTCTCATCACAAAAATACTTCAGGCTGGTGATGTGTTTGTGTTCCCGGAAGGTCTTGTGCATTTCCAATACAATGTTGGATATACTACTGCAGTCGCCTTTTCAGCTTTTAGCAGCCAAAACCCAGGAACTGTTACCCTTGCCAACACGGTATTTGGATCAAGGCCTCACATCTCTAGTGACATTCTTACAAAGGCTTTCCTAGTCGATAAGCAAGCAATCTACAACATACAGGCAAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

465

Amino Acids

49.72

Weight (kDa)

7.13

Isoelectric Point (pI)

29.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 61 - 207 3e-51 Cupin
Cupin_2 PF07883 96 - 168 1.6e-07 Cupin domain
Cupin_1 PF00190 310 - 456 2.1e-47 Cupin
Cupin_2 PF07883 352 - 418 1.5e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 609
AccII CGCG 1 cut(s) 1083
AciI CCGC 1 cut(s) 494
AclWI GGATC 3 cut(s) 92, 568, 1318
AcsI RAATTY 2 cut(s) 708, 996
AcuI CTGAAG 4 cut(s) 62, 157, 407, 1157
AcvI CACGTG 1 cut(s) 971
AdeI CACNNNGTG 1 cut(s) 478
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 3 cut(s) 122, 373, 984
AflIII ACRYGT 2 cut(s) 127, 970
AgsI TTSAA 5 cut(s) 219, 359, 830, 905, 1109
AhlI ACTAGT 1 cut(s) 457
AjnI CCWGG 4 cut(s) 250, 526, 1000, 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 651, 683
AleI CACNNNNGTG 1 cut(s) 416
AluBI AGCT 6 cut(s) 44, 148, 335, 509, 863, 1259
AluI AGCT 6 cut(s) 44, 148, 335, 509, 863, 1259
Alw26I GTCTC 1 cut(s) 1160
AlwI GGATC 3 cut(s) 92, 568, 1318
AlwNI CAGNNNCTG 1 cut(s) 1283
AoxI GGCC 3 cut(s) 3, 756, 1316
ApeKI GCWGC 7 cut(s) 506, 516, 863, 1046, 1085, 1113, 1266
ApoI RAATTY 2 cut(s) 708, 996
AspLEI GCGC 1 cut(s) 1085
AspS9I GGNCC 3 cut(s) 139, 654, 889
AsuC2I CCSGG 1 cut(s) 1199
AsuHPI GGTGA 6 cut(s) 176, 235, 376, 1000, 1126, 1193
AsuNHI GCTAGC 1 cut(s) 775
AvaII GGWCC 3 cut(s) 139, 654, 889
BbrPI CACGTG 1 cut(s) 971
BbsI GAAGAC 1 cut(s) 818
BbvI GCAGC 7 cut(s) 518, 528, 850, 1033, 1072, 1125, 1278
BccI CCATC 2 cut(s) 109, 725
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BciT130I CCWGG 4 cut(s) 252, 528, 1002, 1278
BclI TGATCA 1 cut(s) 675
BcnI CCSGG 1 cut(s) 1199
BcoDI GTCTC 1 cut(s) 1160
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 8 cut(s) 149, 278, 330, 458, 729, 776, 1329, 1355
BfmI CTRYAG 3 cut(s) 864, 922, 1242
BisI GCNGC 7 cut(s) 507, 517, 864, 1047, 1086, 1114, 1267
BlpI GCTNAGC 1 cut(s) 859
BlsI GCNGC 7 cut(s) 508, 518, 865, 1048, 1087, 1115, 1268
Bme1390I CCNGG 5 cut(s) 252, 528, 1002, 1199, 1278
Bme18I GGWCC 3 cut(s) 139, 654, 889
BmgT120I GGNCC 3 cut(s) 139, 654, 889
BmiI GGNNCC 4 cut(s) 141, 365, 656, 891
BmrFI CCNGG 5 cut(s) 252, 528, 1002, 1199, 1278
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 4 cut(s) 68, 959, 1029, 1044
BmtI GCTAGC 1 cut(s) 779
BmuI ACTGGG 1 cut(s) 443
BoxI GACNNNNGTC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 818
Bpu1102I GCTNAGC 1 cut(s) 859
BpuMI CCSGG 1 cut(s) 1199
Bsa29I ATCGAT 1 cut(s) 1038
BsaAI YACGTR 1 cut(s) 971
BsaJI CCNNGG 2 cut(s) 299, 1276
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 373, 984
Bse1I ACTGG 2 cut(s) 449, 634
Bse3DI GCAATG 4 cut(s) 501, 540, 804, 1026
BseBI CCWGG 4 cut(s) 252, 528, 1002, 1278
BseCI ATCGAT 1 cut(s) 1038
BseDI CCNNGG 2 cut(s) 299, 1276
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 3 cut(s) 122, 373, 984
BseMI GCAATG 4 cut(s) 501, 540, 804, 1026
BseMII CTCAG 2 cut(s) 503, 810
BseNI ACTGG 2 cut(s) 449, 634
BseXI GCAGC 7 cut(s) 518, 528, 850, 1033, 1072, 1125, 1278
Bsh1236I CGCG 1 cut(s) 1083
BshFI GGCC 3 cut(s) 5, 758, 1318
BshVI ATCGAT 1 cut(s) 1038
BsiSI CCGG 1 cut(s) 1199
BslFI GGGAC 1 cut(s) 640
BslI CCNNNNNNNGG 3 cut(s) 122, 373, 984
BsmAI GTCTC 1 cut(s) 1160
BsmBI CGTCTC 1 cut(s) 1160
BsmFI GGGAC 1 cut(s) 640
BsmI GAATGC 2 cut(s) 599, 959
BsnI GGCC 3 cut(s) 5, 758, 1318
Bsp143I GATC 5 cut(s) 97, 560, 642, 675, 1310
Bsp1720I GCTNAGC 1 cut(s) 859
Bsp19I CCATGG 1 cut(s) 299
BspACI CCGC 1 cut(s) 494
BspANI GGCC 3 cut(s) 5, 758, 1318
BspCNI CTCAG 2 cut(s) 504, 811
BspDI ATCGAT 1 cut(s) 1038
BspFNI CGCG 1 cut(s) 1083
BspHI TCATGA 1 cut(s) 792
BspLI GGNNCC 4 cut(s) 141, 365, 656, 891
BspMAI CTGCAG 2 cut(s) 868, 1246
BspOI GCTAGC 1 cut(s) 779
BspPI GGATC 3 cut(s) 92, 568, 1318
BsrDI GCAATG 4 cut(s) 501, 540, 804, 1026
BsrI ACTGG 2 cut(s) 449, 634
BssECI CCNNGG 2 cut(s) 299, 1276
BssMI GATC 5 cut(s) 97, 560, 642, 675, 1310
BssT1I CCWWGG 1 cut(s) 299
Bst2UI CCWGG 4 cut(s) 252, 528, 1002, 1278
Bst4CI ACNGT 6 cut(s) 112, 352, 1102, 1154, 1285, 1303
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 2 cut(s) 32, 956
BstBAI YACGTR 1 cut(s) 971
BstC8I GCNNGC 7 cut(s) 28, 193, 570, 777, 943, 1033, 1368
BstDEI CTNAG 3 cut(s) 512, 819, 859
BstDSI CCRYGG 1 cut(s) 299
BstEII GGTNACC 1 cut(s) 223
BstENI CCTNNNNNAGG 1 cut(s) 982
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 1 cut(s) 1083
BstHHI GCGC 1 cut(s) 1085
BstKTI GATC 5 cut(s) 100, 563, 645, 678, 1313
BstMAI GTCTC 1 cut(s) 1160
BstMBI GATC 5 cut(s) 97, 560, 642, 675, 1310
BstNI CCWGG 4 cut(s) 252, 528, 1002, 1278
BstNSI RCATGY 2 cut(s) 30, 131
BstPAI GACNNNNGTC 1 cut(s) 69
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 5 cut(s) 250, 526, 1000, 1197, 1276
BstSFI CTRYAG 3 cut(s) 864, 922, 1242
BstUI CGCG 1 cut(s) 1083
BstV1I GCAGC 7 cut(s) 518, 528, 850, 1033, 1072, 1125, 1278
BstV2I GAAGAC 1 cut(s) 818
BstXI CCANNNNNNTGG 1 cut(s) 811
Bsu15I ATCGAT 1 cut(s) 1038
BsuRI GGCC 3 cut(s) 5, 758, 1318
BsuTUI ATCGAT 1 cut(s) 1038
BtgI CCRYGG 1 cut(s) 299
BtgZI GCGATG 1 cut(s) 447
BtsCI GGATG 1 cut(s) 325
BtsI GCAGTG 2 cut(s) 558, 873
BtsIMutI CAGTG 3 cut(s) 181, 558, 873
Cac8I GCNNGC 7 cut(s) 28, 193, 570, 777, 943, 1033, 1368
CaiI CAGNNNCTG 1 cut(s) 1283
CciI TCATGA 1 cut(s) 792
CfoI GCGC 1 cut(s) 1085
Cfr13I GGNCC 3 cut(s) 139, 654, 889
ClaI ATCGAT 1 cut(s) 1038
Csp6I GTAC 1 cut(s) 113
CviAII CATG 5 cut(s) 27, 128, 300, 793, 1050
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 3 cut(s) 512, 819, 859
DpnI GATC 5 cut(s) 99, 562, 644, 677, 1312
DpnII GATC 5 cut(s) 97, 560, 642, 675, 1310
DraIII CACNNNGTG 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 299
Eco147I AGGCCT 1 cut(s) 1318
Eco47I GGWCC 3 cut(s) 139, 654, 889
Eco57I CTGAAG 4 cut(s) 62, 157, 407, 1157
Eco72I CACGTG 1 cut(s) 971
Eco91I GGTNACC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 982
EcoO109I RGGNCCY 2 cut(s) 139, 889
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 4 cut(s) 250, 526, 1000, 1276
EcoT14I CCWWGG 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 299
Esp3I CGTCTC 1 cut(s) 1160
FaeI CATG 5 cut(s) 30, 131, 303, 796, 1053
FaqI GGGAC 1 cut(s) 640
FatI CATG 5 cut(s) 26, 127, 299, 792, 1049
FauNDI CATATG 1 cut(s) 647
FbaI TGATCA 1 cut(s) 675
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 7 cut(s) 507, 517, 864, 1047, 1086, 1114, 1267
FokI GGATG 1 cut(s) 312
Fsp4HI GCNGC 7 cut(s) 507, 517, 864, 1047, 1086, 1114, 1267
FspBI CTAG 8 cut(s) 149, 278, 330, 458, 729, 776, 1329, 1355
GlaI GCGC 1 cut(s) 1084
GluI GCNGC 7 cut(s) 507, 517, 864, 1047, 1086, 1114, 1267
HaeIII GGCC 3 cut(s) 5, 758, 1318
HapII CCGG 1 cut(s) 1199
HhaI GCGC 1 cut(s) 1085
Hin1II CATG 5 cut(s) 30, 131, 303, 796, 1053
Hin6I GCGC 1 cut(s) 1083
HinP1I GCGC 1 cut(s) 1083
HincII GTYRAC 2 cut(s) 610, 1060
HindII GTYRAC 2 cut(s) 610, 1060
HinfI GANTC 4 cut(s) 247, 736, 823, 1005
HpaI GTTAAC 1 cut(s) 1060
HpaII CCGG 1 cut(s) 1199
HphI GGTGA 6 cut(s) 176, 235, 376, 1000, 1126, 1193
Hpy166II GTNNAC 6 cut(s) 179, 610, 929, 968, 991, 1060
Hpy188I TCNGA 3 cut(s) 64, 426, 513
Hpy188III TCNNGA 7 cut(s) 80, 346, 395, 762, 793, 830, 1096
Hpy8I GTNNAC 6 cut(s) 179, 610, 929, 968, 991, 1060
HpyAV CCTTC 4 cut(s) 353, 421, 1103, 1196
HpyCH4III ACNGT 6 cut(s) 112, 352, 1102, 1154, 1285, 1303
HpyCH4IV ACGT 1 cut(s) 970
HpyF3I CTNAG 3 cut(s) 512, 819, 859
HpySE526I ACGT 1 cut(s) 970
Hsp92II CATG 5 cut(s) 30, 131, 303, 796, 1053
HspAI GCGC 1 cut(s) 1083
Ksp22I TGATCA 1 cut(s) 675
KspAI GTTAAC 1 cut(s) 1060
Kzo9I GATC 5 cut(s) 97, 560, 642, 675, 1310
LmnI GCTCC 3 cut(s) 319, 363, 806
Lsp1109I GCAGC 7 cut(s) 518, 528, 850, 1033, 1072, 1125, 1278
LweI GCATC 4 cut(s) 68, 959, 1029, 1044
MaeI CTAG 8 cut(s) 149, 278, 330, 458, 729, 776, 1329, 1355
MaeII ACGT 1 cut(s) 970
MaeIII GTNAC 6 cut(s) 223, 382, 973, 1132, 1285, 1331
MalI GATC 5 cut(s) 99, 562, 644, 677, 1312
MboI GATC 5 cut(s) 97, 560, 642, 675, 1310
MboII GAAGA 4 cut(s) 68, 160, 163, 818
MluCI AATT 4 cut(s) 666, 708, 744, 996
MlyI GAGTC 2 cut(s) 832, 999
MmeI TCCRAC 3 cut(s) 493, 1164, 1210
MseI TTAA 4 cut(s) 258, 489, 702, 1059
MslI CAYNNNNRTG 1 cut(s) 416
MspI CCGG 1 cut(s) 1199
MspR9I CCNGG 5 cut(s) 252, 528, 1002, 1199, 1278
Mva1269I GAATGC 2 cut(s) 599, 959
MvaI CCWGG 4 cut(s) 252, 528, 1002, 1278
MvnI CGCG 1 cut(s) 1083
NciI CCSGG 1 cut(s) 1199
NcoI CCATGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 647
NdeII GATC 5 cut(s) 97, 560, 642, 675, 1310
NheI GCTAGC 1 cut(s) 775
NlaIII CATG 5 cut(s) 30, 131, 303, 796, 1053
NlaIV GGNNCC 4 cut(s) 141, 365, 656, 891
NmuCI GTSAC 5 cut(s) 223, 382, 973, 1132, 1331
NspI RCATGY 2 cut(s) 30, 131
OliI CACNNNNGTG 1 cut(s) 416
PaeI GCATGC 1 cut(s) 30
PagI TCATGA 1 cut(s) 792
PceI AGGCCT 1 cut(s) 1318
PciI ACATGT 1 cut(s) 127
PctI GAATGC 2 cut(s) 599, 959
PfeI GAWTC 2 cut(s) 247, 736
PfoI TCCNGGA 3 cut(s) 250, 1000, 1197
PkrI GCNGC 7 cut(s) 508, 518, 865, 1048, 1087, 1115, 1268
PleI GAGTC 2 cut(s) 831, 999
PmaCI CACGTG 1 cut(s) 971
PmlI CACGTG 1 cut(s) 971
PpsI GAGTC 2 cut(s) 831, 999
Ppu21I YACGTR 1 cut(s) 971
PpuMI RGGWCCY 2 cut(s) 139, 889
PscI ACATGT 1 cut(s) 127
PshAI GACNNNNGTC 1 cut(s) 69
Psp5II RGGWCCY 2 cut(s) 139, 889
Psp6I CCWGG 4 cut(s) 250, 526, 1000, 1276
PspCI CACGTG 1 cut(s) 971
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 4 cut(s) 250, 526, 1000, 1276
PspN4I GGNNCC 4 cut(s) 141, 365, 656, 891
PspPI GGNCC 3 cut(s) 139, 654, 889
PspPPI RGGWCCY 2 cut(s) 139, 889
PstI CTGCAG 2 cut(s) 868, 1246
PstNI CAGNNNCTG 1 cut(s) 1283
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
RseI CAYNNNNRTG 1 cut(s) 416
SalI GTCGAC 1 cut(s) 608
SaqAI TTAA 4 cut(s) 258, 489, 702, 1059
SatI GCNGC 7 cut(s) 507, 517, 864, 1047, 1086, 1114, 1267
Sau3AI GATC 5 cut(s) 97, 560, 642, 675, 1310
Sau96I GGNCC 3 cut(s) 139, 654, 889
SchI GAGTC 2 cut(s) 832, 999
ScrFI CCNGG 5 cut(s) 252, 528, 1002, 1199, 1278
SfaNI GCATC 4 cut(s) 68, 959, 1029, 1044
SfcI CTRYAG 3 cut(s) 864, 922, 1242
SinI GGWCC 3 cut(s) 139, 654, 889
SmiMI CAYNNNNRTG 1 cut(s) 416
SpeI ACTAGT 1 cut(s) 457
SphI GCATGC 1 cut(s) 30
Sse9I AATT 4 cut(s) 666, 708, 744, 996
SseBI AGGCCT 1 cut(s) 1318
SsiI CCGC 1 cut(s) 494
SspMI CTAG 8 cut(s) 149, 278, 330, 458, 729, 776, 1329, 1355
StuI AGGCCT 1 cut(s) 1318
StyD4I CCNGG 5 cut(s) 250, 526, 1000, 1197, 1276
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 6 cut(s) 112, 352, 1102, 1154, 1285, 1303
TaiI ACGT 1 cut(s) 973
TaqI TCGA 6 cut(s) 288, 609, 670, 763, 1038, 1359
TasI AATT 4 cut(s) 666, 708, 744, 996
TfiI GAWTC 2 cut(s) 247, 736
Tru1I TTAA 4 cut(s) 258, 489, 702, 1059
Tru9I TTAA 4 cut(s) 258, 489, 702, 1059
TscAI CASTG 3 cut(s) 181, 558, 873
TseFI GTSAC 5 cut(s) 223, 382, 973, 1132, 1331
TseI GCWGC 7 cut(s) 506, 516, 863, 1046, 1085, 1113, 1266
Tsp45I GTSAC 5 cut(s) 223, 382, 973, 1132, 1331
TspRI CASTG 3 cut(s) 181, 558, 873
VpaK11BI GGWCC 3 cut(s) 139, 654, 889
XagI CCTNNNNNAGG 1 cut(s) 982
XapI RAATTY 2 cut(s) 708, 996
XceI RCATGY 2 cut(s) 30, 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 1226
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 8 cut(s) 149, 278, 330, 458, 729, 776, 1329, 1355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.