Rorug04G0443600

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000812
Physical Location & Seq
Reverse (-)
59957111 .. 59961219
4109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0443600.1

Sequence Viewer

Length: 1038 bp
ATGGGATCTCCCTCAGTCTCTCACTTTGTCAACTTGACACTTGTTGATCTTCCTGGGCTTACAAAAGTTTCTGTTGAGGGTCAGCCCGATAGTATTGTGCAGGACATTGAAAATATGGTTCGCTCCTACATTGAGAAGCCCAACAGTATAATTTCAGCAATTTCACCTCAAGATCTTGCTACATCTGATGCAATTAAAATCCCTTGTGAAGTGGATCCAATAGACCCAAGCAGGAAGCTGCTACATGGTCGGGGCCTCCAAAGGCTAGGAGGGATTCGACAGATATTGAAATATATGGGATGCAAGGAATCCCACCTGATGTCTTGGCTGCACATTATGGAGAGGAAGAATACGACGCCCCGTCAAAAATGGCTAAATGGAAATCCCATCAACTCAGCTTGTTGGCGGTGTGGTGCCAGATACAATTCATCGGTCCCAATGCCTCATAACGGTTGGCAAGTTGCACCCCGTCCCCAGCCTTGGTATCCACAGCCTCTGGCAGTCTCTGGTCCTCCGCCTTCCGGATTGGGTTATTCACAACAGCCATTATTTCCTGTGCAGCCACCTCTACCATCAACAACATCCCCTGGACTTCAATCTTCACATTTTGAACATACCTTATTTACGGCACGCAAGCGGGTTCTAAAGTTCTTTAGTAGATCCGAGGACATGATGGAATGTAAACATGACAGAGCTACTGGTTCGATAAATTGTGCCCCATGTTTTGATGGTGAAGACTACATGCAATGGAAGATCATGATGAGGATGTTTCTCTATTCTCAAGATGAGAATATGTGGAACATTGTTGAAACTGGATGGGAACATCTAACCAAGCCTGACGACTCAAAGAAGGAAGAAGGGACATCTACAAGAGTTCTTAAGCCTAGGCAGGAGTGGACACAAGAAGAGGTTCGGAACAGAAGTTGTGATTTCAAGGCACAAAATTTCTTATTCATAGCTTTATCCAAGAAAAAGGTTATCAAAGTTATGAAGGCAACAAAAAGGTTAGAGGCCAGAAACTTCAGAGACTTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

345

Amino Acids

39.37

Weight (kDa)

9.2

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_N PF00350 9 - 74 3.7e-08 Dynamin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 413
AccIII TCCGGA 1 cut(s) 521
AciI CCGC 3 cut(s) 406, 515, 637
AclWI GGATC 4 cut(s) 13, 209, 222, 654
AcsI RAATTY 1 cut(s) 943
AcuI CTGAAG 1 cut(s) 1006
AcyI GRCGYC 1 cut(s) 356
AfiI CCNNNNNNNGG 3 cut(s) 449, 480, 521
AflII CTTAAG 1 cut(s) 878
AgsI TTSAA 6 cut(s) 110, 289, 596, 611, 809, 934
AhdI GACNNNNNGTC 1 cut(s) 360
AjnI CCWGG 2 cut(s) 52, 586
AluBI AGCT 4 cut(s) 238, 398, 695, 959
AluI AGCT 4 cut(s) 238, 398, 695, 959
Alw26I GTCTC 3 cut(s) 22, 508, 1020
AlwI GGATC 4 cut(s) 13, 209, 222, 654
AlwNI CAGNNNCTG 2 cut(s) 496, 506
Aor13HI TCCGGA 1 cut(s) 521
AoxI GGCC 2 cut(s) 253, 1011
ApeKI GCWGC 3 cut(s) 238, 328, 559
ApoI RAATTY 1 cut(s) 943
Asp700I GAANNNNTTC 1 cut(s) 909
AspA2I CCTAGG 1 cut(s) 884
AspS9I GGNCC 3 cut(s) 253, 433, 509
AsuHPI GGTGA 2 cut(s) 156, 743
AvaII GGWCC 2 cut(s) 433, 509
AvrII CCTAGG 1 cut(s) 884
BaeGI GKGCMC 1 cut(s) 718
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 413
BbsI GAAGAC 1 cut(s) 741
BbvI GCAGC 3 cut(s) 225, 315, 571
BccI CCATC 5 cut(s) 395, 580, 667, 722, 810
BceAI ACGGC 1 cut(s) 642
BciT130I CCWGG 2 cut(s) 54, 588
BciVI GTATCC 1 cut(s) 495
BcoDI GTCTC 3 cut(s) 22, 508, 1020
BfaI CTAG 2 cut(s) 266, 885
BfrI CTTAAG 1 cut(s) 878
BfuI GTATCC 1 cut(s) 495
BglII AGATCT 1 cut(s) 172
BisI GCNGC 3 cut(s) 239, 329, 560
BlnI CCTAGG 1 cut(s) 884
BlsI GCNGC 3 cut(s) 240, 330, 561
Bme1390I CCNGG 2 cut(s) 54, 588
Bme18I GGWCC 2 cut(s) 433, 509
BmeRI GACNNNNNGTC 1 cut(s) 360
BmgT120I GGNCC 3 cut(s) 253, 433, 509
BmiI GGNNCC 4 cut(s) 216, 254, 415, 435
BmrFI CCNGG 2 cut(s) 54, 588
BmsI GCATC 2 cut(s) 178, 290
BpiI GAAGAC 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 153, 765
BsaHI GRCGYC 1 cut(s) 356
BsaJI CCNNGG 5 cut(s) 53, 479, 586, 663, 884
BsaWI WCCGGW 1 cut(s) 521
Bsc4I CCNNNNNNNGG 3 cut(s) 449, 480, 521
Bse1I ACTGG 2 cut(s) 703, 817
Bse3DI GCAATG 1 cut(s) 752
BseAI TCCGGA 1 cut(s) 521
BseBI CCWGG 2 cut(s) 54, 588
BseDI CCNNGG 5 cut(s) 53, 479, 586, 663, 884
BseGI GGATG 4 cut(s) 305, 581, 771, 821
BseLI CCNNNNNNNGG 3 cut(s) 449, 480, 521
BseMI GCAATG 1 cut(s) 752
BseMII CTCAG 2 cut(s) 27, 408
BseNI ACTGG 2 cut(s) 703, 817
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 3 cut(s) 225, 315, 571
BseYI CCCAGC 1 cut(s) 474
BsgI GTGCAG 3 cut(s) 119, 314, 578
BshFI GGCC 2 cut(s) 255, 1013
BshNI GGYRCC 1 cut(s) 413
BsiSI CCGG 1 cut(s) 522
BslFI GGGAC 3 cut(s) 419, 456, 874
BslI CCNNNNNNNGG 3 cut(s) 449, 480, 521
BsmAI GTCTC 3 cut(s) 22, 508, 1020
BsmFI GGGAC 3 cut(s) 419, 456, 874
BsnI GGCC 2 cut(s) 255, 1013
Bsp1286I GDGCHC 1 cut(s) 718
Bsp13I TCCGGA 1 cut(s) 521
Bsp143I GATC 6 cut(s) 5, 46, 172, 214, 659, 753
BspACI CCGC 3 cut(s) 406, 515, 637
BspANI GGCC 2 cut(s) 255, 1013
BspCNI CTCAG 2 cut(s) 26, 407
BspEI TCCGGA 1 cut(s) 521
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 4 cut(s) 216, 254, 415, 435
BspPI GGATC 4 cut(s) 13, 209, 222, 654
BspT107I GGYRCC 1 cut(s) 413
BspTI CTTAAG 1 cut(s) 878
BsrDI GCAATG 1 cut(s) 752
BsrI ACTGG 2 cut(s) 703, 817
BssECI CCNNGG 5 cut(s) 53, 479, 586, 663, 884
BssMI GATC 6 cut(s) 5, 46, 172, 214, 659, 753
BssNI GRCGYC 1 cut(s) 356
BssT1I CCWWGG 2 cut(s) 479, 884
Bst2UI CCWGG 2 cut(s) 54, 588
Bst4CI ACNGT 2 cut(s) 146, 452
Bst6I CTCTTC 1 cut(s) 900
BstACI GRCGYC 1 cut(s) 356
BstAFI CTTAAG 1 cut(s) 878
BstC8I GCNNGC 2 cut(s) 631, 635
BstDEI CTNAG 2 cut(s) 13, 394
BstF5I GGATG 4 cut(s) 305, 581, 771, 821
BstKTI GATC 6 cut(s) 8, 49, 175, 217, 662, 756
BstMAI GTCTC 3 cut(s) 22, 508, 1020
BstMBI GATC 6 cut(s) 5, 46, 172, 214, 659, 753
BstNI CCWGG 2 cut(s) 54, 588
BstNSI RCATGY 1 cut(s) 745
BstSCI CCNGG 2 cut(s) 52, 586
BstSLI GKGCMC 1 cut(s) 718
BstV1I GCAGC 3 cut(s) 225, 315, 571
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 4 cut(s) 5, 172, 214, 659
BstYI RGATCY 4 cut(s) 5, 172, 214, 659
BsuI GTATCC 1 cut(s) 495
BsuRI GGCC 2 cut(s) 255, 1013
BtsCI GGATG 4 cut(s) 305, 581, 771, 821
Cac8I GCNNGC 2 cut(s) 631, 635
CaiI CAGNNNCTG 2 cut(s) 496, 506
CciI TCATGA 1 cut(s) 756
Cfr13I GGNCC 3 cut(s) 253, 433, 509
CseI GACGC 1 cut(s) 364
CviAII CATG 6 cut(s) 245, 670, 686, 720, 742, 757
DdeI CTNAG 2 cut(s) 13, 394
DpnI GATC 6 cut(s) 7, 48, 174, 216, 661, 755
DpnII GATC 6 cut(s) 5, 46, 172, 214, 659, 753
DriI GACNNNNNGTC 1 cut(s) 360
Eam1104I CTCTTC 1 cut(s) 900
Eam1105I GACNNNNNGTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 900
EciI GGCGGA 1 cut(s) 504
Eco130I CCWWGG 2 cut(s) 479, 884
Eco47I GGWCC 2 cut(s) 433, 509
Eco57I CTGAAG 1 cut(s) 1006
EcoO109I RGGNCCY 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 52, 586
EcoT14I CCWWGG 2 cut(s) 479, 884
ErhI CCWWGG 2 cut(s) 479, 884
FaeI CATG 6 cut(s) 248, 673, 689, 723, 745, 760
FaqI GGGAC 3 cut(s) 419, 456, 874
FatI CATG 6 cut(s) 244, 669, 685, 719, 741, 756
FauI CCCGC 1 cut(s) 630
Fnu4HI GCNGC 3 cut(s) 239, 329, 560
FokI GGATG 4 cut(s) 312, 568, 778, 828
Fsp4HI GCNGC 3 cut(s) 239, 329, 560
FspBI CTAG 2 cut(s) 266, 885
GluI GCNGC 3 cut(s) 239, 329, 560
GsaI CCCAGC 1 cut(s) 478
HaeIII GGCC 2 cut(s) 255, 1013
HapII CCGG 1 cut(s) 522
HgaI GACGC 1 cut(s) 364
Hin1I GRCGYC 1 cut(s) 356
Hin1II CATG 6 cut(s) 248, 673, 689, 723, 745, 760
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 3 cut(s) 274, 308, 842
HpaII CCGG 1 cut(s) 522
HphI GGTGA 2 cut(s) 156, 743
Hpy166II GTNNAC 3 cut(s) 31, 683, 897
Hpy188I TCNGA 4 cut(s) 187, 664, 915, 1025
Hpy188III TCNNGA 5 cut(s) 170, 522, 757, 782, 1035
Hpy8I GTNNAC 3 cut(s) 31, 683, 897
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 4 cut(s) 528, 844, 851, 985
HpyCH4III ACNGT 2 cut(s) 146, 452
HpyCH4V TGCA 7 cut(s) 100, 191, 303, 331, 464, 559, 745
HpyF3I CTNAG 2 cut(s) 13, 394
Hsp92I GRCGYC 1 cut(s) 356
Hsp92II CATG 6 cut(s) 248, 673, 689, 723, 745, 760
Kpn2I TCCGGA 1 cut(s) 521
Kzo9I GATC 6 cut(s) 5, 46, 172, 214, 659, 753
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 3 cut(s) 225, 315, 571
LweI GCATC 2 cut(s) 178, 290
MaeI CTAG 2 cut(s) 266, 885
MalI GATC 6 cut(s) 7, 48, 174, 216, 661, 755
MboI GATC 6 cut(s) 5, 46, 172, 214, 659, 753
MboII GAAGA 7 cut(s) 41, 358, 591, 746, 763, 866, 917
MflI RGATCY 4 cut(s) 5, 172, 214, 659
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 6 cut(s) 150, 159, 192, 424, 709, 943
MlyI GAGTC 1 cut(s) 836
MroI TCCGGA 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 909
MseI TTAA 2 cut(s) 195, 879
MspCI CTTAAG 1 cut(s) 878
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 2 cut(s) 54, 588
MvaI CCWGG 2 cut(s) 54, 588
NdeII GATC 6 cut(s) 5, 46, 172, 214, 659, 753
NlaIII CATG 6 cut(s) 248, 673, 689, 723, 745, 760
NlaIV GGNNCC 4 cut(s) 216, 254, 415, 435
NspI RCATGY 1 cut(s) 745
PagI TCATGA 1 cut(s) 756
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 2 cut(s) 274, 308
PkrI GCNGC 3 cut(s) 240, 330, 561
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 52, 586
PspFI CCCAGC 1 cut(s) 474
PspGI CCWGG 2 cut(s) 52, 586
PspN4I GGNNCC 4 cut(s) 216, 254, 415, 435
PspPI GGNCC 3 cut(s) 253, 433, 509
PstNI CAGNNNCTG 2 cut(s) 496, 506
PsuI RGATCY 4 cut(s) 5, 172, 214, 659
SaqAI TTAA 2 cut(s) 195, 879
SatI GCNGC 3 cut(s) 239, 329, 560
Sau3AI GATC 6 cut(s) 5, 46, 172, 214, 659, 753
Sau96I GGNCC 3 cut(s) 253, 433, 509
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 2 cut(s) 54, 588
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 2 cut(s) 178, 290
SinI GGWCC 2 cut(s) 433, 509
SmlI CTYRAG 3 cut(s) 168, 780, 878
SmoI CTYRAG 3 cut(s) 168, 780, 878
Sse9I AATT 6 cut(s) 150, 159, 192, 424, 709, 943
SsiI CCGC 3 cut(s) 406, 515, 637
SspMI CTAG 2 cut(s) 266, 885
StyD4I CCNGG 2 cut(s) 52, 586
StyI CCWWGG 2 cut(s) 479, 884
TaaI ACNGT 2 cut(s) 146, 452
TaqI TCGA 2 cut(s) 277, 704
TaqII GACCGA 1 cut(s) 421
TasI AATT 6 cut(s) 150, 159, 192, 424, 709, 943
TfiI GAWTC 2 cut(s) 274, 308
Tru1I TTAA 2 cut(s) 195, 879
Tru9I TTAA 2 cut(s) 195, 879
TseI GCWGC 3 cut(s) 238, 328, 559
TspDTI ATGAA 3 cut(s) 417, 943, 1004
Vha464I CTTAAG 1 cut(s) 878
VpaK11BI GGWCC 2 cut(s) 433, 509
XapI RAATTY 1 cut(s) 943
XceI RCATGY 1 cut(s) 745
XmaJI CCTAGG 1 cut(s) 884
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 2 cut(s) 266, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.