Prupe.4G095200_v2.0.a1

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000812
Physical Location & Seq
Reverse (-)
4786669 .. 4787437
769 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G095200.1

Sequence Viewer

Length: 648 bp
ATGGCCTCTCAGTTTCTGTTATTGGCATTCCTTGCTATAACTTGTGCCATTGCTTCGGCATCTGACCCGAGTTCCCTTCAGGATTTCTGTGTGGCTGATTCAAAAGCCTCAGTACTAGTGAATGGCTTTGTCTGCAAGGATTCTAAGCTTGTTGATGCCAATGACTTCTTCTTCAGTGGGCTTCACCTAGCAGGAAACACCTCGAATGCAGTTGGTTCACGCGTGACCCCTGTTAACGCAGTCCAAATTCCAGGACTTAATACTCTTGGCATCTCCATTGTGCGGATCGATTATGCCCCGTGGGGTATTAATCCTCCACACACACATCCACGAGCCACAGAAGTTTTGACAGTCCTGGAAGGGAGCCTCAAAGTTGGTTTTGTCACCTCCAACCCTGAAAACCACCTCATCACCAAGGTGCTTCGGAAGGGTGATGTGTTTGTGTTCCCAGTAGGCCTTGTGCATTTCCAACAGAATGTCGGATATGGTAATGCAGTTGCCATTGCAGCTCTCAACAGCCAAAATCCAGGTGTTATTACCGTTGCAAATGCAGTATTTGGATCAAAGCCGGACATCTCTGCTGATGTTCTTGCAAAGGCTTTCCAAGTGGATAAGAACAGAGTCTACAACTTCCAGTCAAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

216

Amino Acids

22.8

Weight (kDa)

7.76

Isoelectric Point (pI)

13.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 624
AccII CGCG 1 cut(s) 222
AciI CCGC 1 cut(s) 283
AclWI GGATC 2 cut(s) 293, 568
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 2 cut(s) 62, 157
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 1 cut(s) 282
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 1 cut(s) 102
AhlI ACTAGT 1 cut(s) 115
AjnI CCWGG 3 cut(s) 250, 354, 526
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AleI CACNNNNGTG 1 cut(s) 416
AluBI AGCT 2 cut(s) 148, 509
AluI AGCT 2 cut(s) 148, 509
AlwI GGATC 2 cut(s) 293, 568
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 67
AoxI GGCC 2 cut(s) 3, 454
ApeKI GCWGC 1 cut(s) 506
ApoI RAATTY 1 cut(s) 246
AseI ATTAAT 1 cut(s) 309
AsuHPI GGTGA 4 cut(s) 176, 376, 403, 443
AvaI CYCGRG 1 cut(s) 67
BauI CACGAG 1 cut(s) 330
BbvI GCAGC 1 cut(s) 518
BciT130I CCWGG 3 cut(s) 252, 356, 528
BcuI ACTAGT 1 cut(s) 115
BfaI CTAG 2 cut(s) 116, 188
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
BmcAI AGTACT 1 cut(s) 114
Bme1390I CCNGG 3 cut(s) 252, 356, 528
BmeT110I CYCGRG 1 cut(s) 67
BmiI GGNNCC 1 cut(s) 365
BmrFI CCNGG 3 cut(s) 252, 356, 528
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 3 cut(s) 68, 145, 279
BmuI ACTGGG 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 299, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse1I ACTGG 2 cut(s) 449, 634
Bse3DI GCAATG 2 cut(s) 48, 501
BseBI CCWGG 3 cut(s) 252, 356, 528
BseCI ATCGAT 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 299, 414
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMI GCAATG 2 cut(s) 48, 501
BseMII CTCAG 2 cut(s) 23, 123
BseNI ACTGG 2 cut(s) 449, 634
BseXI GCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 5, 456
BshVI ATCGAT 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 67
BsiSI CCGG 1 cut(s) 569
BslI CCNNNNNNNGG 1 cut(s) 282
BsmI GAATGC 2 cut(s) 26, 211
BsnI GGCC 2 cut(s) 5, 456
BsoBI CYCGRG 1 cut(s) 67
Bsp143I GATC 2 cut(s) 285, 560
BspACI CCGC 1 cut(s) 283
BspANI GGCC 2 cut(s) 5, 456
BspCNI CTCAG 2 cut(s) 22, 122
BspDI ATCGAT 1 cut(s) 288
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 365
BspPI GGATC 2 cut(s) 293, 568
BsrDI GCAATG 2 cut(s) 48, 501
BsrI ACTGG 2 cut(s) 449, 634
BssECI CCNNGG 2 cut(s) 299, 414
BssMI GATC 2 cut(s) 285, 560
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 414
Bst2BI CACGAG 1 cut(s) 330
Bst2UI CCWGG 3 cut(s) 252, 356, 528
Bst4CI ACNGT 2 cut(s) 352, 541
BstAPI GCANNNNNTGC 1 cut(s) 32
BstDEI CTNAG 3 cut(s) 9, 109, 144
BstDSI CCRYGG 1 cut(s) 299
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 1 cut(s) 222
BstKTI GATC 2 cut(s) 288, 563
BstMBI GATC 2 cut(s) 285, 560
BstMWI GCNNNNNNNGC 3 cut(s) 32, 132, 506
BstNI CCWGG 3 cut(s) 252, 356, 528
BstSCI CCNGG 3 cut(s) 250, 354, 526
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 518
Bsu15I ATCGAT 1 cut(s) 288
BsuRI GGCC 2 cut(s) 5, 456
BsuTUI ATCGAT 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 299
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 181
CaiI CAGNNNCTG 1 cut(s) 16
ClaI ATCGAT 1 cut(s) 288
Csp6I GTAC 1 cut(s) 113
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 3 cut(s) 9, 109, 144
DpnI GATC 2 cut(s) 287, 562
DpnII GATC 2 cut(s) 285, 560
Eco130I CCWWGG 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 456
Eco57I CTGAAG 2 cut(s) 62, 157
Eco88I CYCGRG 1 cut(s) 67
EcoRII CCWGG 3 cut(s) 250, 354, 526
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FaiI YATR 3 cut(s) 38, 294, 486
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 1 cut(s) 312
Fsp4HI GCNGC 1 cut(s) 507
FspBI CTAG 2 cut(s) 116, 188
GluI GCNGC 1 cut(s) 507
HaeIII GGCC 2 cut(s) 5, 456
HapII CCGG 1 cut(s) 569
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 3 cut(s) 98, 140, 621
HpaI GTTAAC 1 cut(s) 235
HpaII CCGG 1 cut(s) 569
HphI GGTGA 4 cut(s) 176, 376, 403, 443
Hpy166II GTNNAC 3 cut(s) 218, 235, 625
Hpy188I TCNGA 3 cut(s) 64, 426, 482
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 3 cut(s) 218, 235, 625
HpyAV CCTTC 3 cut(s) 86, 353, 421
HpyCH4III ACNGT 2 cut(s) 352, 541
HpyCH4V TGCA 8 cut(s) 135, 209, 463, 494, 506, 545, 551, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 132, 506
HpyF3I CTNAG 3 cut(s) 9, 109, 144
KspAI GTTAAC 1 cut(s) 235
Kzo9I GATC 2 cut(s) 285, 560
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 1 cut(s) 518
LweI GCATC 3 cut(s) 68, 145, 279
MaeI CTAG 2 cut(s) 116, 188
MaeIII GTNAC 2 cut(s) 223, 382
MalI GATC 2 cut(s) 287, 562
MboI GATC 2 cut(s) 285, 560
MboII GAAGA 2 cut(s) 160, 163
MluCI AATT 1 cut(s) 246
MluI ACGCGT 1 cut(s) 220
MlyI GAGTC 1 cut(s) 630
MmeI TCCRAC 3 cut(s) 414, 460, 493
MnlI CCTC 7 cut(s) 16, 118, 211, 324, 377, 397, 416
MseI TTAA 3 cut(s) 234, 258, 309
MslI CAYNNNNRTG 1 cut(s) 416
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 3 cut(s) 252, 356, 528
Mva1269I GAATGC 2 cut(s) 26, 211
MvaI CCWGG 3 cut(s) 252, 356, 528
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 32, 132, 506
NdeII GATC 2 cut(s) 285, 560
NlaIV GGNNCC 1 cut(s) 365
NmuCI GTSAC 2 cut(s) 223, 382
OliI CACNNNNGTG 1 cut(s) 416
PceI AGGCCT 1 cut(s) 456
PctI GAATGC 2 cut(s) 26, 211
PfeI GAWTC 2 cut(s) 98, 140
PfoI TCCNGGA 2 cut(s) 250, 354
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 1 cut(s) 629
PpsI GAGTC 1 cut(s) 629
PshBI ATTAAT 1 cut(s) 309
Psp6I CCWGG 3 cut(s) 250, 354, 526
PspGI CCWGG 3 cut(s) 250, 354, 526
PspN4I GGNNCC 1 cut(s) 365
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
RseI CAYNNNNRTG 1 cut(s) 416
SaqAI TTAA 3 cut(s) 234, 258, 309
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 2 cut(s) 285, 560
ScaI AGTACT 1 cut(s) 114
SchI GAGTC 1 cut(s) 630
ScrFI CCNGG 3 cut(s) 252, 356, 528
SetI ASST 8 cut(s) 150, 189, 203, 389, 408, 420, 511, 532
SfaNI GCATC 3 cut(s) 68, 145, 279
SmiMI CAYNNNNRTG 1 cut(s) 416
SpeI ACTAGT 1 cut(s) 115
Sse9I AATT 1 cut(s) 246
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 1 cut(s) 283
SspMI CTAG 2 cut(s) 116, 188
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 3 cut(s) 250, 354, 526
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 352, 541
TaqI TCGA 2 cut(s) 203, 288
TasI AATT 1 cut(s) 246
TatI WGTACW 1 cut(s) 112
TfiI GAWTC 2 cut(s) 98, 140
Tru1I TTAA 3 cut(s) 234, 258, 309
Tru9I TTAA 3 cut(s) 234, 258, 309
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 2 cut(s) 223, 382
TseI GCWGC 1 cut(s) 506
Tsp45I GTSAC 2 cut(s) 223, 382
TspRI CASTG 1 cut(s) 181
VspI ATTAAT 1 cut(s) 309
XapI RAATTY 1 cut(s) 246
XmiI GTMKAC 1 cut(s) 624
XspI CTAG 2 cut(s) 116, 188
ZrmI AGTACT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.