Rh5AG129700

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000812
Physical Location & Seq
Reverse (-)
12874875 .. 12875632
758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG129700.1

Sequence Viewer

Length: 654 bp
ATGGCCTTTAAGTTTCTGTTGTTGGCATTCCTTGCCATAAGTGCTTGTTCCATTGCTTTGGCTTCTGACCCAAGTTCTCTTCAGGATTTCTGTGTTGCGGACCCAACTGCAGGCTCAGTACCAGTGAATGGACATGCCTGCAAGGACCCCAAGGTTGTCGAAGCCAATGACTTCTTCTTCAGTGGACTTCACCTAGCAGGCAACACGTCAAACCCTGCTGGTTCAAAGGTGACCCCTGCTAATGTTGCCCAGATTCCAGGACTTAATACTTTTGGTATCTCCCTAGTTCGTATCGACTATGCACCATGGGGTATCAATGCTCCACACACTCATCCTAGAGCTACTGAAATCTTGACAGTCCTTGAAGGGAGCCTCCAAGTGGGTTTTGTCACCTCCAACCCCGAAAACAGACTTATCACAAAGGTGCTTCAGAAGGGCGATGTGTTTGTGTTCCCAGTAGGACTTGTGCATTTCCAACACAATGTTGGAAATGTTAATGCGGTTGCCATTGCAGCTCTGAGCAGCCAAAATCCAGGTGCTATTACCATTGCCAATGCAGTGTTTGGATCAAAGCCTGCCATTTCTGCTGATGTTCTTGCAAAGGCATTCCAAGTGGACAAGGACACAATCTATGATCTCCAGTCCAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

217

Amino Acids

22.76

Weight (kDa)

6.27

Isoelectric Point (pI)

27.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 63 - 209 4.7e-52 Cupin
Cupin_2 PF07883 98 - 170 4.2e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 128
AciI CCGC 2 cut(s) 98, 500
AclWI GGATC 1 cut(s) 574
AcuI CTGAAG 3 cut(s) 65, 163, 413
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 3 cut(s) 110, 128, 379
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 225, 365
AjiI CACGTC 1 cut(s) 207
AjnI CCWGG 2 cut(s) 256, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AleI CACNNNNGTG 1 cut(s) 422
AluBI AGCT 2 cut(s) 341, 515
AluI AGCT 2 cut(s) 341, 515
AlwI GGATC 1 cut(s) 574
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 512, 522
AspS9I GGNCC 2 cut(s) 100, 145
AsuHPI GGTGA 3 cut(s) 182, 241, 382
AvaII GGWCC 2 cut(s) 100, 145
BbvI GCAGC 2 cut(s) 524, 534
BciT130I CCWGG 2 cut(s) 258, 534
BfaI CTAG 4 cut(s) 194, 284, 336, 652
BfmI CTRYAG 1 cut(s) 108
BisI GCNGC 2 cut(s) 513, 523
BlsI GCNGC 2 cut(s) 514, 524
Bme1390I CCNGG 2 cut(s) 258, 534
Bme18I GGWCC 2 cut(s) 100, 145
BmgBI CACGTC 1 cut(s) 207
BmgT120I GGNCC 2 cut(s) 100, 145
BmiI GGNNCC 3 cut(s) 102, 147, 371
BmrFI CCNGG 2 cut(s) 258, 534
BmrI ACTGGG 1 cut(s) 449
BmuI ACTGGG 1 cut(s) 449
BpmI CTGGAG 1 cut(s) 623
BsaJI CCNNGG 2 cut(s) 150, 305
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 128, 379
Bse1I ACTGG 3 cut(s) 122, 455, 640
Bse3DI GCAATG 3 cut(s) 51, 507, 546
BseBI CCWGG 2 cut(s) 258, 534
BseDI CCNNGG 2 cut(s) 150, 305
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 3 cut(s) 110, 128, 379
BseMI GCAATG 3 cut(s) 51, 507, 546
BseMII CTCAG 2 cut(s) 129, 509
BseNI ACTGG 3 cut(s) 122, 455, 640
BseXI GCAGC 2 cut(s) 524, 534
BshFI GGCC 1 cut(s) 5
BslI CCNNNNNNNGG 3 cut(s) 110, 128, 379
BsmI GAATGC 2 cut(s) 26, 605
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 566, 634
Bsp19I CCATGG 1 cut(s) 305
BspACI CCGC 2 cut(s) 98, 500
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 128, 510
BspLI GGNNCC 3 cut(s) 102, 147, 371
BspMAI CTGCAG 1 cut(s) 112
BspPI GGATC 1 cut(s) 574
BsrDI GCAATG 3 cut(s) 51, 507, 546
BsrI ACTGG 3 cut(s) 122, 455, 640
BssECI CCNNGG 2 cut(s) 150, 305
BssMI GATC 2 cut(s) 566, 634
BssT1I CCWWGG 2 cut(s) 150, 305
Bst2UI CCWGG 2 cut(s) 258, 534
Bst4CI ACNGT 1 cut(s) 358
Bst6I CTCTTC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 4 cut(s) 112, 139, 199, 576
BstDEI CTNAG 2 cut(s) 115, 518
BstDSI CCRYGG 1 cut(s) 305
BstEII GGTNACC 1 cut(s) 229
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 2 cut(s) 569, 637
BstMBI GATC 2 cut(s) 566, 634
BstMWI GCNNNNNNNGC 5 cut(s) 32, 41, 245, 512, 584
BstNI CCWGG 2 cut(s) 258, 534
BstNSI RCATGY 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 256, 532
BstSFI CTRYAG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 524, 534
BstXI CCANNNNNNTGG 1 cut(s) 58
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 305
BtgZI GCGATG 1 cut(s) 453
BtrI CACGTC 1 cut(s) 207
BtsCI GGATG 1 cut(s) 331
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 3 cut(s) 129, 187, 564
Cac8I GCNNGC 4 cut(s) 112, 139, 199, 576
Cfr13I GGNCC 2 cut(s) 100, 145
Csp6I GTAC 1 cut(s) 119
CviAII CATG 2 cut(s) 134, 306
CviJI RGCY 9 cut(s) 5, 62, 114, 164, 341, 372, 515, 525, 574
CviKI_1 RGCY 9 cut(s) 5, 62, 114, 164, 341, 372, 515, 525, 574
CviQI GTAC 1 cut(s) 119
DdeI CTNAG 2 cut(s) 115, 518
DpnI GATC 2 cut(s) 568, 636
DpnII GATC 2 cut(s) 566, 634
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 150, 305
Eco47I GGWCC 2 cut(s) 100, 145
Eco57I CTGAAG 3 cut(s) 65, 163, 413
Eco91I GGTNACC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 145
EcoO65I GGTNACC 1 cut(s) 229
EcoRII CCWGG 2 cut(s) 256, 532
EcoT14I CCWWGG 2 cut(s) 150, 305
ErhI CCWWGG 2 cut(s) 150, 305
FaeI CATG 2 cut(s) 137, 309
FaiI YATR 5 cut(s) 38, 135, 300, 307, 633
FatI CATG 2 cut(s) 133, 305
Fnu4HI GCNGC 2 cut(s) 513, 523
FokI GGATG 1 cut(s) 318
Fsp4HI GCNGC 2 cut(s) 513, 523
FspBI CTAG 4 cut(s) 194, 284, 336, 652
GluI GCNGC 2 cut(s) 513, 523
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 2 cut(s) 137, 309
HinfI GANTC 1 cut(s) 253
HphI GGTGA 3 cut(s) 182, 241, 382
Hpy166II GTNNAC 2 cut(s) 185, 616
Hpy188I TCNGA 3 cut(s) 67, 432, 519
Hpy188III TCNNGA 2 cut(s) 83, 352
Hpy8I GTNNAC 2 cut(s) 185, 616
HpyAV CCTTC 2 cut(s) 359, 427
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 7 cut(s) 110, 141, 302, 469, 512, 557, 599
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 41, 245, 512, 584
HpyF3I CTNAG 2 cut(s) 115, 518
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 2 cut(s) 137, 309
Kzo9I GATC 2 cut(s) 566, 634
LmnI GCTCC 2 cut(s) 325, 369
Lsp1109I GCAGC 2 cut(s) 524, 534
MaeI CTAG 4 cut(s) 194, 284, 336, 652
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 229, 388
MalI GATC 2 cut(s) 568, 636
MboI GATC 2 cut(s) 566, 634
MboII GAAGA 3 cut(s) 71, 166, 169
MmeI TCCRAC 3 cut(s) 420, 466, 499
MnlI CCTC 2 cut(s) 383, 403
MseI TTAA 3 cut(s) 9, 264, 495
MslI CAYNNNNRTG 1 cut(s) 422
MspR9I CCNGG 2 cut(s) 258, 534
Mva1269I GAATGC 2 cut(s) 26, 605
MvaI CCWGG 2 cut(s) 258, 534
MwoI GCNNNNNNNGC 5 cut(s) 32, 41, 245, 512, 584
NcoI CCATGG 1 cut(s) 305
NdeII GATC 2 cut(s) 566, 634
NlaIII CATG 2 cut(s) 137, 309
NlaIV GGNNCC 3 cut(s) 102, 147, 371
NmuCI GTSAC 2 cut(s) 229, 388
NspI RCATGY 1 cut(s) 137
OliI CACNNNNGTG 1 cut(s) 422
PctI GAATGC 2 cut(s) 26, 605
PfeI GAWTC 1 cut(s) 253
PflMI CCANNNNNTGG 1 cut(s) 128
PfoI TCCNGGA 1 cut(s) 256
PkrI GCNGC 2 cut(s) 514, 524
PpuMI RGGWCCY 1 cut(s) 145
Psp5II RGGWCCY 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 256, 532
PspEI GGTNACC 1 cut(s) 229
PspGI CCWGG 2 cut(s) 256, 532
PspN4I GGNNCC 3 cut(s) 102, 147, 371
PspPI GGNCC 2 cut(s) 100, 145
PspPPI RGGWCCY 1 cut(s) 145
PstI CTGCAG 1 cut(s) 112
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
RseI CAYNNNNRTG 1 cut(s) 422
SaqAI TTAA 3 cut(s) 9, 264, 495
SatI GCNGC 2 cut(s) 513, 523
Sau3AI GATC 2 cut(s) 566, 634
Sau96I GGNCC 2 cut(s) 100, 145
ScrFI CCNGG 2 cut(s) 258, 534
SetI ASST 9 cut(s) 156, 195, 209, 231, 343, 395, 426, 517, 538
SfcI CTRYAG 1 cut(s) 108
SinI GGWCC 2 cut(s) 100, 145
SmiMI CAYNNNNRTG 1 cut(s) 422
SsiI CCGC 2 cut(s) 98, 500
SspMI CTAG 4 cut(s) 194, 284, 336, 652
StyD4I CCNGG 2 cut(s) 256, 532
StyI CCWWGG 2 cut(s) 150, 305
TaaI ACNGT 1 cut(s) 358
TaiI ACGT 1 cut(s) 209
TaqI TCGA 2 cut(s) 159, 294
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 3 cut(s) 9, 264, 495
Tru9I TTAA 3 cut(s) 9, 264, 495
TscAI CASTG 3 cut(s) 129, 187, 564
TseFI GTSAC 2 cut(s) 229, 388
TseI GCWGC 2 cut(s) 512, 522
Tsp45I GTSAC 2 cut(s) 229, 388
TspRI CASTG 3 cut(s) 129, 187, 564
Van91I CCANNNNNTGG 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 100, 145
XceI RCATGY 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XspI CTAG 4 cut(s) 194, 284, 336, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.