RLG00000032278

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000812
Physical Location & Seq
Forward (+)
12987191 .. 13007166
19976 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032278

Sequence Viewer

Length: 861 bp
ATGGCCTCTAAGTTTCTAATTTTGGCATTCGTTGCCATAACTTCTTCCCTTGCTTTTGCTGACCCAAGTTCCCTTCAGGATTTCTGTGTGGCTGATCTGAAAAGCTCAGGCAACACATCAAACCCTGCTGGTTCACGTGTGACCCCTGCGAACGTGGCCCAAATTCCAGGACTTAATACTCTTGGTATCTCCCTTATTCGTATCGACTATGCACCATGGGGCATCAATGCTCCCCACACTCATCCTAGAGCCACTGAAGTTCTAACTGTCCTCGAAGGGACCCTCCAAGTGGGTTTTGTCACTTCCAACCCCGAAAACAGACTTATCACAAAGGTGCTTCAGAAAGGTGATGTGTTTGTTTTCCCAGTAGGACTTGTGCATTTCCAGCACAACGTTGGAAAGGTTAATGCAGTCGCCATTGCCGCTCTCAGCAGCCAAAATCCAGGTGTTATTAGCATCGCCAATTCAGTATTTGGATCTAAGCCTGCTATCTCTGCTGATGTTCTTGCAAAGGCTTTCCAAGTGGATAAGGACACAATCTACAATCTTCAGTCAAATTCTTCTCTATCCGCTGCTGTCATCACCATTCTCTTCAATTCTTGTGAGGTCATCAGAAAAAATCTGATTGAGCGAGGTGATTTGAGGGGACTCTCTGCTCTTTCTCAAACCAGACAGCATAGCTGCTTGTTGATGCTTCTTAATTATCCGGACGACCAATATCTCCAACTCTCTTTGGAATTTTCTTCGAGGAGTAACATTGTGGTTGCTTGTTCAATGGCCGGTGTATTGATTCTGGATGTATTCAAATGTTTTTCGCAGCAGGAGATTAACCATGCTGGCGCTGGTGGGTTGAGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

287

Amino Acids

30.44

Weight (kDa)

7.0

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 33 - 179 2.4e-52 Cupin
Cupin_2 PF07883 69 - 140 5.3e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 425
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 2 cut(s) 423, 570
AclI AACGTT 1 cut(s) 393
AclWI GGATC 1 cut(s) 484
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 3 cut(s) 162, 556, 737
AcuI CTGAAG 4 cut(s) 59, 276, 323, 533
AcvI CACGTG 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 3 cut(s) 595, 774, 805
AjnI CCWGG 2 cut(s) 166, 442
AleI CACNNNNGTG 1 cut(s) 332
AluBI AGCT 3 cut(s) 105, 681, 855
AluI AGCT 3 cut(s) 105, 681, 855
AlwI GGATC 1 cut(s) 484
Aor13HI TCCGGA 1 cut(s) 706
AoxI GGCC 3 cut(s) 3, 156, 777
ApeKI GCWGC 4 cut(s) 432, 572, 681, 817
ApoI RAATTY 3 cut(s) 162, 556, 737
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 2 cut(s) 157, 279
AsuHPI GGTGA 3 cut(s) 359, 574, 647
AvaII GGWCC 1 cut(s) 279
BbrPI CACGTG 1 cut(s) 137
BbvI GCAGC 4 cut(s) 444, 559, 668, 829
BciT130I CCWGG 2 cut(s) 168, 444
BfaI CTAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 843
BisI GCNGC 5 cut(s) 423, 433, 573, 682, 818
BlsI GCNGC 5 cut(s) 424, 434, 574, 683, 819
Bme1390I CCNGG 2 cut(s) 168, 444
Bme18I GGWCC 1 cut(s) 279
BmgT120I GGNCC 2 cut(s) 157, 279
BmiI GGNNCC 2 cut(s) 280, 281
BmrFI CCNGG 2 cut(s) 168, 444
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 3 cut(s) 231, 465, 681
BmuI ACTGGG 1 cut(s) 359
Bpu10I CCTNAGC 1 cut(s) 106
BsaAI YACGTR 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 215
BsaWI WCCGGW 1 cut(s) 706
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse118I RCCGGY 1 cut(s) 779
Bse1I ACTGG 1 cut(s) 365
Bse3DI GCAATG 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 2 cut(s) 168, 444
BseDI CCNNGG 1 cut(s) 215
BseGI GGATG 2 cut(s) 241, 802
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 2 cut(s) 120, 442
BseNI ACTGG 1 cut(s) 365
BseRI GAGGAG 1 cut(s) 763
BseXI GCAGC 4 cut(s) 444, 559, 668, 829
BshFI GGCC 3 cut(s) 5, 158, 779
BsiSI CCGG 2 cut(s) 707, 780
BslFI GGGAC 2 cut(s) 292, 660
BslI CCNNNNNNNGG 1 cut(s) 289
BsmFI GGGAC 2 cut(s) 292, 660
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 3 cut(s) 5, 158, 779
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 2 cut(s) 94, 476
Bsp19I CCATGG 1 cut(s) 215
BspACI CCGC 2 cut(s) 423, 570
BspANI GGCC 3 cut(s) 5, 158, 779
BspCNI CTCAG 2 cut(s) 119, 441
BspEI TCCGGA 1 cut(s) 706
BspLI GGNNCC 2 cut(s) 280, 281
BspPI GGATC 1 cut(s) 484
BsrBI CCGCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 1 cut(s) 779
BsrI ACTGG 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 779
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 2 cut(s) 94, 476
BssT1I CCWWGG 1 cut(s) 215
Bst2UI CCWGG 2 cut(s) 168, 444
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 137
BstC8I GCNNGC 2 cut(s) 486, 838
BstDEI CTNAG 4 cut(s) 9, 106, 428, 480
BstDSI CCRYGG 1 cut(s) 215
BstF5I GGATG 2 cut(s) 241, 802
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 2 cut(s) 97, 479
BstMBI GATC 2 cut(s) 94, 476
BstMWI GCNNNNNNNGC 5 cut(s) 32, 155, 385, 422, 494
BstNI CCWGG 2 cut(s) 168, 444
BstSCI CCNGG 2 cut(s) 166, 442
BstV1I GCAGC 4 cut(s) 444, 559, 668, 829
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
BsuRI GGCC 3 cut(s) 5, 158, 779
BtgI CCRYGG 1 cut(s) 215
BtgZI GCGATG 1 cut(s) 442
BtsCI GGATG 2 cut(s) 241, 802
BtsIMutI CAGTG 1 cut(s) 252
Cac8I GCNNGC 2 cut(s) 486, 838
CfoI GCGC 1 cut(s) 842
Cfr10I RCCGGY 1 cut(s) 779
Cfr13I GGNCC 2 cut(s) 157, 279
CviAII CATG 2 cut(s) 216, 833
DdeI CTNAG 4 cut(s) 9, 106, 428, 480
DpnI GATC 2 cut(s) 96, 478
DpnII GATC 2 cut(s) 94, 476
EaeI YGGCCR 1 cut(s) 777
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Eco130I CCWWGG 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 279
Eco57I CTGAAG 4 cut(s) 59, 276, 323, 533
Eco72I CACGTG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 166, 442
EcoT14I CCWWGG 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 215
FaeI CATG 2 cut(s) 219, 836
FaiI YATR 5 cut(s) 38, 210, 217, 678, 834
FaqI GGGAC 2 cut(s) 292, 660
FatI CATG 2 cut(s) 215, 832
Fnu4HI GCNGC 5 cut(s) 423, 433, 573, 682, 818
FokI GGATG 2 cut(s) 228, 809
Fsp4HI GCNGC 5 cut(s) 423, 433, 573, 682, 818
FspBI CTAG 1 cut(s) 246
GlaI GCGC 1 cut(s) 841
GluI GCNGC 5 cut(s) 423, 433, 573, 682, 818
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 3 cut(s) 5, 158, 779
HapII CCGG 2 cut(s) 707, 780
HhaI GCGC 1 cut(s) 842
Hin1II CATG 2 cut(s) 219, 836
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HinfI GANTC 2 cut(s) 648, 790
HpaII CCGG 2 cut(s) 707, 780
HphI GGTGA 3 cut(s) 359, 574, 647
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 4 cut(s) 99, 342, 614, 624
Hpy188III TCNNGA 3 cut(s) 77, 707, 794
Hpy8I GTNNAC 1 cut(s) 134
HpyAV CCTTC 2 cut(s) 83, 269
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 3 cut(s) 136, 153, 393
HpyCH4V TGCA 4 cut(s) 212, 379, 410, 509
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 155, 385, 422, 494
HpyF3I CTNAG 4 cut(s) 9, 106, 428, 480
HpySE526I ACGT 3 cut(s) 136, 153, 393
Hsp92II CATG 2 cut(s) 219, 836
HspAI GCGC 1 cut(s) 840
KflI GGGWCCC 1 cut(s) 279
Kpn2I TCCGGA 1 cut(s) 706
Kzo9I GATC 2 cut(s) 94, 476
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 4 cut(s) 444, 559, 668, 829
LweI GCATC 3 cut(s) 231, 465, 681
MaeI CTAG 1 cut(s) 246
MaeII ACGT 3 cut(s) 136, 153, 393
MaeIII GTNAC 3 cut(s) 139, 298, 752
MalI GATC 2 cut(s) 96, 478
MbiI CCGCTC 1 cut(s) 425
MboI GATC 2 cut(s) 94, 476
MboII GAAGA 5 cut(s) 36, 539, 552, 583, 735
MflI RGATCY 1 cut(s) 476
MluCI AATT 7 cut(s) 18, 162, 463, 556, 595, 700, 737
MlyI GAGTC 1 cut(s) 642
MmeI TCCRAC 3 cut(s) 330, 376, 748
MnlI CCTC 7 cut(s) 16, 281, 293, 598, 626, 636, 741
MroI TCCGGA 1 cut(s) 706
MseI TTAA 4 cut(s) 174, 405, 699, 828
MslI CAYNNNNRTG 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 2 cut(s) 707, 780
MspR9I CCNGG 2 cut(s) 168, 444
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 2 cut(s) 168, 444
MwoI GCNNNNNNNGC 5 cut(s) 32, 155, 385, 422, 494
NcoI CCATGG 1 cut(s) 215
NdeII GATC 2 cut(s) 94, 476
NlaIII CATG 2 cut(s) 219, 836
NlaIV GGNNCC 2 cut(s) 280, 281
NmuCI GTSAC 2 cut(s) 139, 298
OliI CACNNNNGTG 1 cut(s) 332
PctI GAATGC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 790
PfoI TCCNGGA 1 cut(s) 166
PkrI GCNGC 5 cut(s) 424, 434, 574, 683, 819
PleI GAGTC 1 cut(s) 642
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 1 cut(s) 642
Ppu21I YACGTR 1 cut(s) 137
PpuMI RGGWCCY 1 cut(s) 279
Psp1406I AACGTT 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 279
Psp6I CCWGG 2 cut(s) 166, 442
PspCI CACGTG 1 cut(s) 137
PspGI CCWGG 2 cut(s) 166, 442
PspN4I GGNNCC 2 cut(s) 280, 281
PspPI GGNCC 2 cut(s) 157, 279
PspPPI RGGWCCY 1 cut(s) 279
PsuI RGATCY 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 4 cut(s) 174, 405, 699, 828
SatI GCNGC 5 cut(s) 423, 433, 573, 682, 818
Sau3AI GATC 2 cut(s) 94, 476
Sau96I GGNCC 2 cut(s) 157, 279
SchI GAGTC 1 cut(s) 642
ScrFI CCNGG 2 cut(s) 168, 444
SfaNI GCATC 3 cut(s) 231, 465, 681
SinI GGWCC 1 cut(s) 279
SmiMI CAYNNNNRTG 1 cut(s) 332
Sse9I AATT 7 cut(s) 18, 162, 463, 556, 595, 700, 737
SsiI CCGC 2 cut(s) 423, 570
SspMI CTAG 1 cut(s) 246
StyD4I CCNGG 2 cut(s) 166, 442
StyI CCWWGG 1 cut(s) 215
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 3 cut(s) 139, 156, 396
TaqI TCGA 3 cut(s) 204, 273, 746
TasI AATT 7 cut(s) 18, 162, 463, 556, 595, 700, 737
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 1 cut(s) 790
Tru1I TTAA 4 cut(s) 174, 405, 699, 828
Tru9I TTAA 4 cut(s) 174, 405, 699, 828
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 2 cut(s) 139, 298
TseI GCWGC 4 cut(s) 432, 572, 681, 817
Tsp45I GTSAC 2 cut(s) 139, 298
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 279
XapI RAATTY 3 cut(s) 162, 556, 737
XcmI CCANNNNNNNNNTGG 2 cut(s) 392, 839
XspI CTAG 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.