MD10G1248200.v1.1

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000812
Physical Location & Seq
Forward (+)
34111264 .. 34111833
570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1248200.v1.1.491

Sequence Viewer

Length: 570 bp
ATGCTGATAATAATTTTTCTCATCATGGTTTCAGTACTGGTGAATGGTCGTGTTTGCAAGGACCCTAAGCTTGTTGAAGCCAACGATTTTTTCTTCAGTGGGCTTCACCTAGCAGGCAACACATCAAATGCAGTTGGTTCACGTGTGACTCCTGTTAGTGTAGCCCAAATTGGAGGACTTAATACCCTTGGCATCTCCGTTGCGCGTATTGATTTTGTCCCATGGGGTCTTAACCCTCCACATACACACCCTAGAGCTACTGAAATTTTGACAGTCCTGGAAGGGAGCCTCAAAGTTGGTTTTGTCACCTCCAATCCCGAAAACCGCCTCATCAGCAAGGTACTTCAGAAGGGTGATGTGTTTGTTTTCCCAGTAGGCCTTGTGCATTTCCAACAAAATGTTGGACACGGAAATGCAGTTGCCATTGTAGCTCTCAGCAGCCAAAATCCAGGTGTGATTGCCATTGCAAATGCTGTATTTGGATCAAACCCTGATATCCCTGCTGATATTCTTGCCAAGGCTTTCCAAGTGGATACAAACGCAATCTACGGTCTTCAGTCCAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

190

Amino Acids

20.01

Weight (kDa)

7.95

Isoelectric Point (pI)

14.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 35 - 180 7.5e-50 Cupin
Cupin_2 PF07883 76 - 143 5.3e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 205
AciI CCGC 1 cut(s) 325
AclWI GGATC 1 cut(s) 490
AcsI RAATTY 2 cut(s) 264, 563
AcuI CTGAAG 3 cut(s) 79, 329, 539
AcvI CACGTG 1 cut(s) 143
AfaI GTAC 2 cut(s) 36, 342
AflIII ACRYGT 1 cut(s) 142
AgsI TTSAA 1 cut(s) 77
AjnI CCWGG 2 cut(s) 276, 448
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 3 cut(s) 70, 257, 431
AluI AGCT 3 cut(s) 70, 257, 431
AlwI GGATC 1 cut(s) 490
AoxI GGCC 1 cut(s) 376
ApeKI GCWGC 1 cut(s) 438
ApoI RAATTY 2 cut(s) 264, 563
AspLEI GCGC 1 cut(s) 205
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 4 cut(s) 52, 98, 298, 365
AvaII GGWCC 1 cut(s) 61
BbrPI CACGTG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 545
BbvI GCAGC 1 cut(s) 450
BciT130I CCWGG 2 cut(s) 278, 450
BciVI GTATCC 1 cut(s) 526
BfaI CTAG 2 cut(s) 110, 252
BfuI GTATCC 1 cut(s) 526
BisI GCNGC 1 cut(s) 439
BlsI GCNGC 1 cut(s) 440
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 2 cut(s) 278, 450
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 2 cut(s) 63, 287
BmrFI CCNGG 2 cut(s) 278, 450
BmrI ACTGGG 1 cut(s) 365
BmsI GCATC 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 365
BpiI GAAGAC 1 cut(s) 545
Bpu10I CCTNAGC 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 143
BsaJI CCNNGG 3 cut(s) 187, 221, 516
Bse1I ACTGG 2 cut(s) 42, 371
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 2 cut(s) 278, 450
BseDI CCNNGG 3 cut(s) 187, 221, 516
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 1 cut(s) 448
BseNI ACTGG 2 cut(s) 42, 371
BseXI GCAGC 1 cut(s) 450
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 1 cut(s) 378
BslFI GGGAC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 1 cut(s) 378
Bsp143I GATC 1 cut(s) 482
Bsp19I CCATGG 1 cut(s) 221
BspACI CCGC 1 cut(s) 325
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 1 cut(s) 447
BspFNI CGCG 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 63, 287
BspPI GGATC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 2 cut(s) 42, 371
BssECI CCNNGG 3 cut(s) 187, 221, 516
BssMI GATC 1 cut(s) 482
BssT1I CCWWGG 3 cut(s) 187, 221, 516
Bst2UI CCWGG 2 cut(s) 278, 450
Bst4CI ACNGT 2 cut(s) 274, 551
BstBAI YACGTR 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 2 cut(s) 66, 434
BstDSI CCRYGG 1 cut(s) 221
BstFNI CGCG 1 cut(s) 205
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 1 cut(s) 485
BstMBI GATC 1 cut(s) 482
BstMWI GCNNNNNNNGC 2 cut(s) 333, 428
BstNI CCWGG 2 cut(s) 278, 450
BstSCI CCNGG 2 cut(s) 276, 448
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 450
BstV2I GAAGAC 1 cut(s) 545
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 1 cut(s) 378
BtgI CCRYGG 1 cut(s) 221
BtsIMutI CAGTG 1 cut(s) 103
Cac8I GCNNGC 1 cut(s) 115
CfoI GCGC 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 2 cut(s) 35, 341
CviAII CATG 2 cut(s) 25, 222
CviQI GTAC 2 cut(s) 35, 341
DdeI CTNAG 2 cut(s) 66, 434
DpnI GATC 1 cut(s) 484
DpnII GATC 1 cut(s) 482
Eco130I CCWWGG 3 cut(s) 187, 221, 516
Eco147I AGGCCT 1 cut(s) 378
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 61
Eco57I CTGAAG 3 cut(s) 79, 329, 539
Eco72I CACGTG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 61
EcoRII CCWGG 2 cut(s) 276, 448
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 3 cut(s) 187, 221, 516
ErhI CCWWGG 3 cut(s) 187, 221, 516
FaeI CATG 2 cut(s) 28, 225
FaiI YATR 3 cut(s) 26, 223, 243
FaqI GGGAC 1 cut(s) 203
FatI CATG 2 cut(s) 24, 221
Fnu4HI GCNGC 1 cut(s) 439
Fsp4HI GCNGC 1 cut(s) 439
FspBI CTAG 2 cut(s) 110, 252
GlaI GCGC 1 cut(s) 204
GluI GCNGC 1 cut(s) 439
HaeIII GGCC 1 cut(s) 378
HhaI GCGC 1 cut(s) 205
Hin1II CATG 2 cut(s) 28, 225
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 1 cut(s) 148
HphI GGTGA 4 cut(s) 52, 98, 298, 365
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 275, 343
HpyCH4III ACNGT 2 cut(s) 274, 551
HpyCH4IV ACGT 1 cut(s) 142
HpyCH4V TGCA 5 cut(s) 57, 131, 385, 416, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 428
HpyF3I CTNAG 2 cut(s) 66, 434
HpySE526I ACGT 1 cut(s) 142
Hsp92II CATG 2 cut(s) 28, 225
HspAI GCGC 1 cut(s) 203
Kzo9I GATC 1 cut(s) 482
LmnI GCTCC 1 cut(s) 285
Lsp1109I GCAGC 1 cut(s) 450
LweI GCATC 1 cut(s) 201
MaeI CTAG 2 cut(s) 110, 252
MaeII ACGT 1 cut(s) 142
MaeIII GTNAC 2 cut(s) 145, 304
MalI GATC 1 cut(s) 484
MboI GATC 1 cut(s) 482
MboII GAAGA 2 cut(s) 85, 545
MluCI AATT 4 cut(s) 12, 168, 264, 563
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 2 cut(s) 382, 415
MnlI CCTC 5 cut(s) 167, 246, 299, 319, 338
MseI TTAA 2 cut(s) 180, 231
MslI CAYNNNNRTG 1 cut(s) 411
MspR9I CCNGG 2 cut(s) 278, 450
MvaI CCWGG 2 cut(s) 278, 450
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 2 cut(s) 333, 428
NcoI CCATGG 1 cut(s) 221
NdeII GATC 1 cut(s) 482
NlaIII CATG 2 cut(s) 28, 225
NlaIV GGNNCC 2 cut(s) 63, 287
NmuCI GTSAC 2 cut(s) 145, 304
PceI AGGCCT 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 1 cut(s) 440
PleI GAGTC 1 cut(s) 142
PmaCI CACGTG 1 cut(s) 143
PmlI CACGTG 1 cut(s) 143
PpsI GAGTC 1 cut(s) 142
Ppu21I YACGTR 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 2 cut(s) 276, 448
PspCI CACGTG 1 cut(s) 143
PspGI CCWGG 2 cut(s) 276, 448
PspN4I GGNNCC 2 cut(s) 63, 287
PspPI GGNCC 1 cut(s) 61
PspPPI RGGWCCY 1 cut(s) 61
RsaI GTAC 2 cut(s) 36, 342
RsaNI GTAC 2 cut(s) 35, 341
RseI CAYNNNNRTG 1 cut(s) 411
SaqAI TTAA 2 cut(s) 180, 231
SatI GCNGC 1 cut(s) 439
Sau3AI GATC 1 cut(s) 482
Sau96I GGNCC 1 cut(s) 61
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 2 cut(s) 278, 450
SetI ASST 8 cut(s) 72, 111, 145, 259, 311, 342, 433, 454
SfaNI GCATC 1 cut(s) 201
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 411
Sse9I AATT 4 cut(s) 12, 168, 264, 563
SseBI AGGCCT 1 cut(s) 378
SsiI CCGC 1 cut(s) 325
SspMI CTAG 2 cut(s) 110, 252
StuI AGGCCT 1 cut(s) 378
StyD4I CCNGG 2 cut(s) 276, 448
StyI CCWWGG 3 cut(s) 187, 221, 516
TaaI ACNGT 2 cut(s) 274, 551
TaiI ACGT 1 cut(s) 145
TasI AATT 4 cut(s) 12, 168, 264, 563
TatI WGTACW 1 cut(s) 34
Tru1I TTAA 2 cut(s) 180, 231
Tru9I TTAA 2 cut(s) 180, 231
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 2 cut(s) 145, 304
TseI GCWGC 1 cut(s) 438
Tsp45I GTSAC 2 cut(s) 145, 304
TspGWI ACGGA 2 cut(s) 187, 423
TspRI CASTG 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 264, 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XspI CTAG 2 cut(s) 110, 252
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.