RLG00000032279

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000812
Physical Location & Seq
Reverse (-)
13020213 .. 13020967
755 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032279

Sequence Viewer

Length: 651 bp
ATGGCCTTTAAGTTTCTGTTGTTGGCATTCCTTGCCATGAGTGCTTGTTCCATTGCTTTGGCTTCTGACCCAAGTTCTCTTCAGGATTTCTGTGTTGCGGACCCAACAGGCTCAGTACCAGTGAATGGACATGTCTGCAAGGACCCCAAGGTTGTCAAAGCCAATGACTTCTTCTTCAGTGGACTTCACCTAGCAGGCAACACTTCAAACCCTGCTGGTTCAAAGGTGACCCCTGCTAATGTTGCCCAGATTCCAGGACTTAACACTTTTGGTATCTCCCTAGTTCGTATCGACTATGCACCATGGGGTATCAATGCTCCACACACTCATCCTAGAGCTACTGAAATCTTGACAGTCCTTGAAGGGAGCCTCCATGTGGGTTTTGTCACCTCCAACCCCGAAAACAGACTTATCACAAAGGTGCTTCAGAAGGGCGATGTGTTTGTGTTCCCAGTAGGACTTGTGCATTTCCAACACAATGTTGGAAATGTTAATGCGGTCGCCATTGCAGCTCTGAGCAGCCAAAATCCAGGTGCTATTACCATTGCCAATGCAGTGTTTGGATCAAAGGCTGCCATTTCTGCTGATGTTCTTGCAAAGGCATTCCAAGTGGACAAGGACACAATCTATGATCTCCAGTCCAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

217

Amino Acids

22.71

Weight (kDa)

7.05

Isoelectric Point (pI)

25.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 62 - 208 1.3e-51 Cupin
Cupin_2 PF07883 97 - 169 2.2e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 125
AciI CCGC 2 cut(s) 98, 497
AclWI GGATC 1 cut(s) 571
AcuI CTGAAG 3 cut(s) 65, 160, 410
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 2 cut(s) 125, 376
AflIII ACRYGT 1 cut(s) 130
AgsI TTSAA 3 cut(s) 207, 222, 362
AjnI CCWGG 2 cut(s) 253, 529
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 2 cut(s) 338, 512
AluI AGCT 2 cut(s) 338, 512
AlwI GGATC 1 cut(s) 571
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 509, 519, 572
AspS9I GGNCC 2 cut(s) 100, 142
AsuHPI GGTGA 3 cut(s) 179, 238, 379
AvaII GGWCC 2 cut(s) 100, 142
BbvI GCAGC 3 cut(s) 521, 531, 559
BciT130I CCWGG 2 cut(s) 255, 531
BfaI CTAG 4 cut(s) 191, 281, 333, 649
BisI GCNGC 3 cut(s) 510, 520, 573
BlsI GCNGC 3 cut(s) 511, 521, 574
Bme1390I CCNGG 2 cut(s) 255, 531
Bme18I GGWCC 2 cut(s) 100, 142
BmgT120I GGNCC 2 cut(s) 100, 142
BmiI GGNNCC 3 cut(s) 102, 144, 368
BmrFI CCNGG 2 cut(s) 255, 531
BmrI ACTGGG 1 cut(s) 446
BmuI ACTGGG 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 620
BsaJI CCNNGG 2 cut(s) 147, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 376
Bse1I ACTGG 3 cut(s) 119, 452, 637
Bse3DI GCAATG 3 cut(s) 51, 504, 543
BseBI CCWGG 2 cut(s) 255, 531
BseDI CCNNGG 2 cut(s) 147, 302
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 2 cut(s) 125, 376
BseMI GCAATG 3 cut(s) 51, 504, 543
BseMII CTCAG 2 cut(s) 126, 506
BseNI ACTGG 3 cut(s) 119, 452, 637
BseXI GCAGC 3 cut(s) 521, 531, 559
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 501
BslI CCNNNNNNNGG 2 cut(s) 125, 376
BsmI GAATGC 2 cut(s) 26, 602
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 563, 631
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 2 cut(s) 98, 497
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 125, 507
BspLI GGNNCC 3 cut(s) 102, 144, 368
BspPI GGATC 1 cut(s) 571
BsrDI GCAATG 3 cut(s) 51, 504, 543
BsrI ACTGG 3 cut(s) 119, 452, 637
BssECI CCNNGG 2 cut(s) 147, 302
BssMI GATC 2 cut(s) 563, 631
BssT1I CCWWGG 2 cut(s) 147, 302
Bst2UI CCWGG 2 cut(s) 255, 531
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 2 cut(s) 112, 515
BstDSI CCRYGG 1 cut(s) 302
BstEII GGTNACC 1 cut(s) 226
BstF5I GGATG 1 cut(s) 328
BstKTI GATC 2 cut(s) 566, 634
BstMBI GATC 2 cut(s) 563, 631
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 5 cut(s) 32, 41, 242, 509, 581
BstNI CCWGG 2 cut(s) 255, 531
BstNSI RCATGY 1 cut(s) 134
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 253, 529
BstV1I GCAGC 3 cut(s) 521, 531, 559
BstXI CCANNNNNNTGG 1 cut(s) 58
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 302
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 3 cut(s) 126, 184, 561
Cac8I GCNNGC 1 cut(s) 196
Cfr13I GGNCC 2 cut(s) 100, 142
Csp6I GTAC 1 cut(s) 116
CviAII CATG 4 cut(s) 37, 131, 303, 374
CviJI RGCY 9 cut(s) 5, 62, 111, 161, 338, 369, 512, 522, 572
CviKI_1 RGCY 9 cut(s) 5, 62, 111, 161, 338, 369, 512, 522, 572
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 2 cut(s) 112, 515
DpnI GATC 2 cut(s) 565, 633
DpnII GATC 2 cut(s) 563, 631
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 147, 302
Eco47I GGWCC 2 cut(s) 100, 142
Eco57I CTGAAG 3 cut(s) 65, 160, 410
Eco91I GGTNACC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 142
EcoO65I GGTNACC 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 253, 529
EcoT14I CCWWGG 2 cut(s) 147, 302
ErhI CCWWGG 2 cut(s) 147, 302
FaeI CATG 4 cut(s) 40, 134, 306, 377
FaiI YATR 6 cut(s) 38, 132, 297, 304, 375, 630
FatI CATG 4 cut(s) 36, 130, 302, 373
Fnu4HI GCNGC 3 cut(s) 510, 520, 573
FokI GGATG 1 cut(s) 315
Fsp4HI GCNGC 3 cut(s) 510, 520, 573
FspBI CTAG 4 cut(s) 191, 281, 333, 649
GluI GCNGC 3 cut(s) 510, 520, 573
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 4 cut(s) 40, 134, 306, 377
HinfI GANTC 1 cut(s) 250
HphI GGTGA 3 cut(s) 179, 238, 379
Hpy166II GTNNAC 2 cut(s) 182, 613
Hpy188I TCNGA 3 cut(s) 67, 429, 516
Hpy188III TCNNGA 2 cut(s) 83, 349
Hpy8I GTNNAC 2 cut(s) 182, 613
HpyAV CCTTC 2 cut(s) 356, 424
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4V TGCA 6 cut(s) 138, 299, 466, 509, 554, 596
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 41, 242, 509, 581
HpyF3I CTNAG 2 cut(s) 112, 515
Hsp92II CATG 4 cut(s) 40, 134, 306, 377
Kzo9I GATC 2 cut(s) 563, 631
LmnI GCTCC 2 cut(s) 322, 366
Lsp1109I GCAGC 3 cut(s) 521, 531, 559
MaeI CTAG 4 cut(s) 191, 281, 333, 649
MaeIII GTNAC 2 cut(s) 226, 385
MalI GATC 2 cut(s) 565, 633
MboI GATC 2 cut(s) 563, 631
MboII GAAGA 3 cut(s) 71, 163, 166
MmeI TCCRAC 3 cut(s) 417, 463, 496
MnlI CCTC 2 cut(s) 380, 400
MseI TTAA 3 cut(s) 9, 261, 492
MslI CAYNNNNRTG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 255, 531
Mva1269I GAATGC 2 cut(s) 26, 602
MvaI CCWGG 2 cut(s) 255, 531
MwoI GCNNNNNNNGC 5 cut(s) 32, 41, 242, 509, 581
NcoI CCATGG 1 cut(s) 302
NdeII GATC 2 cut(s) 563, 631
NlaIII CATG 4 cut(s) 40, 134, 306, 377
NlaIV GGNNCC 3 cut(s) 102, 144, 368
NmuCI GTSAC 2 cut(s) 226, 385
NspI RCATGY 1 cut(s) 134
OliI CACNNNNGTG 1 cut(s) 419
PciI ACATGT 1 cut(s) 130
PctI GAATGC 2 cut(s) 26, 602
PfeI GAWTC 1 cut(s) 250
PflMI CCANNNNNTGG 1 cut(s) 125
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 3 cut(s) 511, 521, 574
PpuMI RGGWCCY 1 cut(s) 142
PscI ACATGT 1 cut(s) 130
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 2 cut(s) 253, 529
PspEI GGTNACC 1 cut(s) 226
PspGI CCWGG 2 cut(s) 253, 529
PspN4I GGNNCC 3 cut(s) 102, 144, 368
PspPI GGNCC 2 cut(s) 100, 142
PspPPI RGGWCCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 3 cut(s) 9, 261, 492
SatI GCNGC 3 cut(s) 510, 520, 573
Sau3AI GATC 2 cut(s) 563, 631
Sau96I GGNCC 2 cut(s) 100, 142
ScrFI CCNGG 2 cut(s) 255, 531
SetI ASST 8 cut(s) 153, 192, 228, 340, 392, 423, 514, 535
SinI GGWCC 2 cut(s) 100, 142
SmiMI CAYNNNNRTG 1 cut(s) 419
SsiI CCGC 2 cut(s) 98, 497
SspMI CTAG 4 cut(s) 191, 281, 333, 649
StyD4I CCNGG 2 cut(s) 253, 529
StyI CCWWGG 2 cut(s) 147, 302
TaaI ACNGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 291
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 3 cut(s) 9, 261, 492
Tru9I TTAA 3 cut(s) 9, 261, 492
TscAI CASTG 3 cut(s) 126, 184, 561
TseFI GTSAC 2 cut(s) 226, 385
TseI GCWGC 3 cut(s) 509, 519, 572
Tsp45I GTSAC 2 cut(s) 226, 385
TspRI CASTG 3 cut(s) 126, 184, 561
Van91I CCANNNNNTGG 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 100, 142
XceI RCATGY 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XspI CTAG 4 cut(s) 191, 281, 333, 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.