MD10G1247900.v1.1

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000812
Physical Location & Seq
Reverse (-)
34084804 .. 34085626
823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1247900.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGCCTCTCAGCTTCTATTTTTCGCATTCCTTGCCGTAACTTCTTCCATTGCTTTGGCATCTGACCCAAGTTCTCTTCAGGACTTCTGCGTGGCTGATGCAACGAGCCCAGTACTGGTGAATGGTCGTGTTTGCAAGGACCCTAAGCTTGTTGAAGCCAACGATTTCTTCTTCAGTGGGCTTCACCTAGCAGGCAACACATCAAATGCAGTTGGTTCACGTGTGACTCCTGTTAGTGTAGCCCAAATTGGAGGACTTAATACCCTTGGCATCTCCGTTGCGCGTATTGATTTTGCCCCATGGGGTCTTAACCCTCCACATACACACCCTAGAGCTACTGAAATTTTGACAGTACTGGAAGGGAGCCTCAAAGTTGGTTTTGTCACCTCCAATCCTGAAAACCGCCTCATCAGCAAGGTGCTTCAGAAGGGTGATGTGTTTGTTTTCCCAGTAGGCCTTGTGCATTTCCAACAAAATGTTGGACACGGAAATGCAGTTGCCATTGTAGCTCTCAGCAGCCAAAATCCAGGTGTCATTGCCATTGCAAATGCTGTATTTGGATCAAACCCTGATATCCCTGCTGATATTCTTGCAAAGGCTTTCCAAGTGGATACGAACGCAATCTACGGTCTTCAGTCCAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

216

Amino Acids

22.51

Weight (kDa)

6.16

Isoelectric Point (pI)

22.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 61 - 206 1.2e-50 Cupin
Cupin_2 PF07883 97 - 169 3.1e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 283
AciI CCGC 1 cut(s) 403
AclWI GGATC 1 cut(s) 568
AcsI RAATTY 2 cut(s) 342, 641
AcuI CTGAAG 4 cut(s) 62, 157, 407, 617
AcvI CACGTG 1 cut(s) 221
AfaI GTAC 2 cut(s) 114, 354
AfiI CCNNNNNNNGG 1 cut(s) 115
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 1 cut(s) 155
AjnI CCWGG 1 cut(s) 526
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 4 cut(s) 13, 148, 335, 509
AluI AGCT 4 cut(s) 13, 148, 335, 509
AlwI GGATC 1 cut(s) 568
AoxI GGCC 2 cut(s) 3, 454
ApeKI GCWGC 1 cut(s) 516
ApoI RAATTY 2 cut(s) 342, 641
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 4 cut(s) 130, 176, 376, 443
AvaII GGWCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 110
BbrPI CACGTG 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 623
BbvI GCAGC 1 cut(s) 528
BceAI ACGGC 1 cut(s) 20
BciT130I CCWGG 1 cut(s) 528
BciVI GTATCC 1 cut(s) 604
BfaI CTAG 2 cut(s) 188, 330
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 1 cut(s) 517
BlsI GCNGC 1 cut(s) 518
BmcAI AGTACT 2 cut(s) 114, 354
Bme1390I CCNGG 1 cut(s) 528
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 2 cut(s) 141, 365
BmrFI CCNGG 1 cut(s) 528
BmrI ACTGGG 2 cut(s) 104, 443
BmsI GCATC 3 cut(s) 68, 88, 279
BmuI ACTGGG 2 cut(s) 104, 443
BpiI GAAGAC 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 265, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 4 cut(s) 110, 120, 360, 449
Bse3DI GCAATG 3 cut(s) 48, 534, 540
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 2 cut(s) 265, 299
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMI GCAATG 3 cut(s) 48, 534, 540
BseMII CTCAG 2 cut(s) 23, 526
BseNI ACTGG 4 cut(s) 110, 120, 360, 449
BseXI GCAGC 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 2 cut(s) 5, 456
BslI CCNNNNNNNGG 1 cut(s) 115
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 2 cut(s) 5, 456
Bsp1286I GDGCHC 1 cut(s) 110
Bsp143I GATC 1 cut(s) 560
Bsp19I CCATGG 1 cut(s) 299
BspACI CCGC 1 cut(s) 403
BspANI GGCC 2 cut(s) 5, 456
BspCNI CTCAG 2 cut(s) 22, 525
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 141, 365
BspPI GGATC 1 cut(s) 568
BsrDI GCAATG 3 cut(s) 48, 534, 540
BsrI ACTGG 4 cut(s) 110, 120, 360, 449
BssECI CCNNGG 2 cut(s) 265, 299
BssMI GATC 1 cut(s) 560
BssT1I CCWWGG 2 cut(s) 265, 299
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 2 cut(s) 352, 629
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 3 cut(s) 9, 144, 512
BstDSI CCRYGG 1 cut(s) 299
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 1 cut(s) 563
BstMBI GATC 1 cut(s) 560
BstMWI GCNNNNNNNGC 3 cut(s) 32, 411, 506
BstNI CCWGG 1 cut(s) 528
BstSCI CCNGG 1 cut(s) 526
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 528
BstV2I GAAGAC 1 cut(s) 623
BstXI CCANNNNNNTGG 1 cut(s) 55
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 2 cut(s) 5, 456
BtgI CCRYGG 1 cut(s) 299
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 113, 353
CviAII CATG 1 cut(s) 300
CviQI GTAC 2 cut(s) 113, 353
DdeI CTNAG 3 cut(s) 9, 144, 512
DpnI GATC 1 cut(s) 562
DpnII GATC 1 cut(s) 560
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 265, 299
Eco147I AGGCCT 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 110
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 139
Eco57I CTGAAG 4 cut(s) 62, 157, 407, 617
Eco72I CACGTG 1 cut(s) 221
EcoO109I RGGNCCY 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 526
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 2 cut(s) 265, 299
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 2 cut(s) 265, 299
FaeI CATG 1 cut(s) 303
FaiI YATR 2 cut(s) 301, 321
FatI CATG 1 cut(s) 299
Fnu4HI GCNGC 1 cut(s) 517
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 1 cut(s) 517
FspBI CTAG 2 cut(s) 188, 330
GlaI GCGC 1 cut(s) 282
GluI GCNGC 1 cut(s) 517
HaeIII GGCC 2 cut(s) 5, 456
HhaI GCGC 1 cut(s) 283
Hin1II CATG 1 cut(s) 303
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 1 cut(s) 226
HphI GGTGA 4 cut(s) 130, 176, 376, 443
Hpy166II GTNNAC 1 cut(s) 218
Hpy188I TCNGA 2 cut(s) 64, 426
Hpy188III TCNNGA 2 cut(s) 80, 395
Hpy8I GTNNAC 1 cut(s) 218
HpyAV CCTTC 2 cut(s) 353, 421
HpyCH4III ACNGT 2 cut(s) 352, 629
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 7 cut(s) 101, 135, 209, 463, 494, 545, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 411, 506
HpyF3I CTNAG 3 cut(s) 9, 144, 512
HpySE526I ACGT 1 cut(s) 220
Hsp92II CATG 1 cut(s) 303
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 1 cut(s) 560
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 1 cut(s) 528
LweI GCATC 3 cut(s) 68, 88, 279
MaeI CTAG 2 cut(s) 188, 330
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 3 cut(s) 37, 223, 382
MalI GATC 1 cut(s) 562
MboI GATC 1 cut(s) 560
MboII GAAGA 5 cut(s) 36, 68, 160, 163, 623
MhlI GDGCHC 1 cut(s) 110
MluCI AATT 3 cut(s) 246, 342, 641
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 2 cut(s) 460, 493
MnlI CCTC 6 cut(s) 16, 245, 324, 377, 397, 416
MseI TTAA 2 cut(s) 258, 309
MslI CAYNNNNRTG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 1 cut(s) 528
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 3 cut(s) 32, 411, 506
NcoI CCATGG 1 cut(s) 299
NdeII GATC 1 cut(s) 560
NlaIII CATG 1 cut(s) 303
NlaIV GGNNCC 2 cut(s) 141, 365
NmuCI GTSAC 2 cut(s) 223, 382
PceI AGGCCT 1 cut(s) 456
PctI GAATGC 1 cut(s) 26
PkrI GCNGC 1 cut(s) 518
PleI GAGTC 1 cut(s) 220
PmaCI CACGTG 1 cut(s) 221
PmlI CACGTG 1 cut(s) 221
PpsI GAGTC 1 cut(s) 220
Ppu21I YACGTR 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 526
PspCI CACGTG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 2 cut(s) 141, 365
PspPI GGNCC 1 cut(s) 139
PspPPI RGGWCCY 1 cut(s) 139
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
RsaI GTAC 2 cut(s) 114, 354
RsaNI GTAC 2 cut(s) 113, 353
RseI CAYNNNNRTG 1 cut(s) 489
SaqAI TTAA 2 cut(s) 258, 309
SatI GCNGC 1 cut(s) 517
Sau3AI GATC 1 cut(s) 560
Sau96I GGNCC 1 cut(s) 139
ScaI AGTACT 2 cut(s) 114, 354
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 528
SduI GDGCHC 1 cut(s) 110
SetI ASST 9 cut(s) 15, 150, 189, 223, 337, 389, 420, 511, 532
SfaNI GCATC 3 cut(s) 68, 88, 279
SinI GGWCC 1 cut(s) 139
SmiMI CAYNNNNRTG 1 cut(s) 489
Sse9I AATT 3 cut(s) 246, 342, 641
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 1 cut(s) 403
SspMI CTAG 2 cut(s) 188, 330
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 1 cut(s) 526
StyI CCWWGG 2 cut(s) 265, 299
TaaI ACNGT 2 cut(s) 352, 629
TaiI ACGT 1 cut(s) 223
TasI AATT 3 cut(s) 246, 342, 641
TatI WGTACW 2 cut(s) 112, 352
Tru1I TTAA 2 cut(s) 258, 309
Tru9I TTAA 2 cut(s) 258, 309
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 2 cut(s) 223, 382
TseI GCWGC 1 cut(s) 516
Tsp45I GTSAC 2 cut(s) 223, 382
TspGWI ACGGA 2 cut(s) 265, 501
TspRI CASTG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 139
XapI RAATTY 2 cut(s) 342, 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XspI CTAG 2 cut(s) 188, 330
ZrmI AGTACT 2 cut(s) 114, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.