MD05G1269600.v1.1

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000812
Physical Location & Seq
Forward (+)
40471588 .. 40472367
780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1269600.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGCCTCTCAGTTCCTATTTTTCGCATTCCTTGCCGTAACTTTTTCCGTTGCTTTGGCATCGGACCCGAGTTCTCTTCAGGATTTCTGCGTGGCAGATGCAACGAGCTCAGTTCTGGTTAATGGTCGTGTTTGCAAGGACCCTAAGCTTGTTGAAGCCAACGATTTCTTTTTCAGTGGGCTTCACCTAGCAGGCAACACATCAAACGCAGTTGGTTCACGTGTGACTCCTGTTGGTGTAGCTCAAATTGGAGGACTTAATGCCCTTGGCATCACTGCTGCGCGTATTGATTTTGCCGCATGGGGTCTTAACCCTCCACATACACACCCTAGAGCTACTGAGATTTTGACAGTCCTGGAAGGGAGCCTCAAAGTTGGTTTTGTCACCTCGAACACTGAAAACCGCCTCATCACCAAGGTACTTCAGAAGGGTGATGTTTTTGTGTTCCCAGTCGGTCTTGTGCATTTCCAGCAGAATGTTGGAAATGGTAATGCAGTTGCCCTTGTAGCTCTCAGCAGCCAAAATCCAGGTGTCATTACCATTGCAAATGCAGTATTTGGATCAAATCCTGATATCCCTGCTGATATTCTTGCAAAGGCTTTCCAAGTGGATACGAACGCGATCTACAGTCTTCAGTCCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

216

Amino Acids

22.54

Weight (kDa)

6.03

Isoelectric Point (pI)

18.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 61 - 206 8.3e-49 Cupin
Cupin_2 PF07883 104 - 169 1.4e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 283, 620
AciI CCGC 2 cut(s) 297, 403
AclWI GGATC 1 cut(s) 568
AcuI CTGAAG 3 cut(s) 62, 407, 617
AcvI CACGTG 1 cut(s) 221
AfaI GTAC 1 cut(s) 420
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 1 cut(s) 155
AjnI CCWGG 2 cut(s) 354, 526
AluBI AGCT 5 cut(s) 108, 148, 242, 335, 509
AluI AGCT 5 cut(s) 108, 148, 242, 335, 509
Alw21I GWGCWC 1 cut(s) 110
AlwI GGATC 1 cut(s) 568
Ama87I CYCGRG 1 cut(s) 67
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 278, 516
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 64, 139
AsuHPI GGTGA 4 cut(s) 176, 376, 403, 443
AvaI CYCGRG 1 cut(s) 67
AvaII GGWCC 2 cut(s) 64, 139
BanII GRGCYC 1 cut(s) 110
BbrPI CACGTG 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 623
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 265, 528
BceAI ACGGC 1 cut(s) 20
BciT130I CCWGG 2 cut(s) 356, 528
BciVI GTATCC 1 cut(s) 604
BfaI CTAG 2 cut(s) 188, 330
BfmI CTRYAG 1 cut(s) 625
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 3 cut(s) 279, 297, 517
BlsI GCNGC 3 cut(s) 280, 298, 518
Bme1390I CCNGG 2 cut(s) 356, 528
Bme18I GGWCC 2 cut(s) 64, 139
BmeT110I CYCGRG 1 cut(s) 67
BmgT120I GGNCC 2 cut(s) 64, 139
BmiI GGNNCC 3 cut(s) 66, 141, 365
BmrFI CCNGG 2 cut(s) 356, 528
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 3 cut(s) 68, 88, 279
BmuI ACTGGG 1 cut(s) 443
BpiI GAAGAC 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 265, 414
Bse1I ACTGG 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 540
BseBI CCWGG 2 cut(s) 356, 528
BseDI CCNNGG 2 cut(s) 265, 414
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 4 cut(s) 23, 123, 330, 526
BseNI ACTGG 1 cut(s) 449
BseXI GCAGC 2 cut(s) 265, 528
Bsh1236I CGCG 2 cut(s) 283, 620
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 67
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 67
Bsp1286I GDGCHC 1 cut(s) 110
Bsp143I GATC 2 cut(s) 560, 621
BspACI CCGC 2 cut(s) 297, 403
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 22, 122, 331, 525
BspFNI CGCG 2 cut(s) 283, 620
BspLI GGNNCC 3 cut(s) 66, 141, 365
BspPI GGATC 1 cut(s) 568
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 265, 414
BssMI GATC 2 cut(s) 560, 621
BssT1I CCWWGG 2 cut(s) 265, 414
Bst2UI CCWGG 2 cut(s) 356, 528
Bst4CI ACNGT 2 cut(s) 352, 629
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 5 cut(s) 9, 109, 144, 339, 512
BstFNI CGCG 2 cut(s) 283, 620
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 2 cut(s) 563, 624
BstMBI GATC 2 cut(s) 560, 621
BstMWI GCNNNNNNNGC 3 cut(s) 32, 469, 506
BstNI CCWGG 2 cut(s) 356, 528
BstSCI CCNGG 2 cut(s) 354, 526
BstSFI CTRYAG 1 cut(s) 625
BstUI CGCG 2 cut(s) 283, 620
BstV1I GCAGC 2 cut(s) 265, 528
BstV2I GAAGAC 1 cut(s) 623
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 1 cut(s) 5
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 181, 273, 393
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 64, 139
Csp6I GTAC 1 cut(s) 419
CviAII CATG 1 cut(s) 300
CviQI GTAC 1 cut(s) 419
DdeI CTNAG 5 cut(s) 9, 109, 144, 339, 512
DpnI GATC 2 cut(s) 562, 623
DpnII GATC 2 cut(s) 560, 621
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 2 cut(s) 265, 414
Eco24I GRGCYC 1 cut(s) 110
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 2 cut(s) 64, 139
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 3 cut(s) 62, 407, 617
Eco72I CACGTG 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 67
EcoICRI GAGCTC 1 cut(s) 108
EcoO109I RGGNCCY 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 354, 526
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 2 cut(s) 265, 414
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 2 cut(s) 265, 414
FaeI CATG 1 cut(s) 303
FaiI YATR 2 cut(s) 301, 321
FatI CATG 1 cut(s) 299
Fnu4HI GCNGC 3 cut(s) 279, 297, 517
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 3 cut(s) 279, 297, 517
FspBI CTAG 2 cut(s) 188, 330
GlaI GCGC 1 cut(s) 282
GluI GCNGC 3 cut(s) 279, 297, 517
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 283
Hin1II CATG 1 cut(s) 303
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 1 cut(s) 226
HphI GGTGA 4 cut(s) 176, 376, 403, 443
Hpy166II GTNNAC 1 cut(s) 218
Hpy188I TCNGA 2 cut(s) 64, 426
Hpy188III TCNNGA 2 cut(s) 80, 569
Hpy8I GTNNAC 1 cut(s) 218
HpyAV CCTTC 2 cut(s) 353, 421
HpyCH4III ACNGT 2 cut(s) 352, 629
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 7 cut(s) 101, 135, 463, 494, 545, 551, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 469, 506
HpyF3I CTNAG 5 cut(s) 9, 109, 144, 339, 512
HpySE526I ACGT 1 cut(s) 220
Hsp92II CATG 1 cut(s) 303
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 2 cut(s) 560, 621
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 2 cut(s) 265, 528
LweI GCATC 3 cut(s) 68, 88, 279
MaeI CTAG 2 cut(s) 188, 330
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 3 cut(s) 37, 223, 382
MalI GATC 2 cut(s) 562, 623
MboI GATC 2 cut(s) 560, 621
MboII GAAGA 2 cut(s) 68, 623
MhlI GDGCHC 1 cut(s) 110
MluCI AATT 1 cut(s) 246
MlyI GAGTC 1 cut(s) 220
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 6 cut(s) 16, 245, 324, 377, 397, 416
MseI TTAA 3 cut(s) 120, 258, 309
MspR9I CCNGG 2 cut(s) 356, 528
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 2 cut(s) 356, 528
MvnI CGCG 2 cut(s) 283, 620
MwoI GCNNNNNNNGC 3 cut(s) 32, 469, 506
NdeII GATC 2 cut(s) 560, 621
NlaIII CATG 1 cut(s) 303
NlaIV GGNNCC 3 cut(s) 66, 141, 365
NmuCI GTSAC 2 cut(s) 223, 382
PctI GAATGC 1 cut(s) 26
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 3 cut(s) 280, 298, 518
PleI GAGTC 1 cut(s) 220
PmaCI CACGTG 1 cut(s) 221
PmlI CACGTG 1 cut(s) 221
PpsI GAGTC 1 cut(s) 220
Ppu21I YACGTR 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 2 cut(s) 354, 526
PspCI CACGTG 1 cut(s) 221
PspGI CCWGG 2 cut(s) 354, 526
PspN4I GGNNCC 3 cut(s) 66, 141, 365
PspPI GGNCC 2 cut(s) 64, 139
PspPPI RGGWCCY 1 cut(s) 139
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 3 cut(s) 120, 258, 309
SatI GCNGC 3 cut(s) 279, 297, 517
Sau3AI GATC 2 cut(s) 560, 621
Sau96I GGNCC 2 cut(s) 64, 139
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 2 cut(s) 356, 528
SduI GDGCHC 1 cut(s) 110
SfaNI GCATC 3 cut(s) 68, 88, 279
SfcI CTRYAG 1 cut(s) 625
SinI GGWCC 2 cut(s) 64, 139
Sse9I AATT 1 cut(s) 246
SsiI CCGC 2 cut(s) 297, 403
SspMI CTAG 2 cut(s) 188, 330
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 2 cut(s) 354, 526
StyI CCWWGG 2 cut(s) 265, 414
TaaI ACNGT 2 cut(s) 352, 629
TaiI ACGT 1 cut(s) 223
TaqI TCGA 1 cut(s) 389
TaqII GACCGA 1 cut(s) 443
TasI AATT 1 cut(s) 246
TauI GCSGC 1 cut(s) 299
Tru1I TTAA 3 cut(s) 120, 258, 309
Tru9I TTAA 3 cut(s) 120, 258, 309
TscAI CASTG 3 cut(s) 181, 280, 400
TseFI GTSAC 2 cut(s) 223, 382
TseI GCWGC 2 cut(s) 278, 516
Tsp45I GTSAC 2 cut(s) 223, 382
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 3 cut(s) 181, 280, 400
VpaK11BI GGWCC 2 cut(s) 64, 139
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XspI CTAG 2 cut(s) 188, 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.