Rorug05G0034300

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000812
Physical Location & Seq
Forward (+)
2363280 .. 2399238
35959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0034300.1

Sequence Viewer

Length: 1581 bp
ATGGTTTCCTCAATATTTCCATCGATATTACTCTCTCTCATTCTGTTACTCCTGACCATCATCCCTCAAACCCTTGCTTTTCTATACCATGAATGCGTAGATGACAAAGGCAATTACACACCTAATAGCACGTATGAAGAAAACCTCAAAACGCTTCTCTCCTCTCTTCCCGCTAGCAACGGTAACGGCTATGGCTTTTACAATTCTTCCTATGGCAGCGGGGAAAGCTCTAAAGATCAAGTTCATGTAATTGCGCTTTGCAGAGGCGATGTTCCGCTGGTTGAATGCCGTAGCTGCCTCAATGATTCAACAAAGGCTCTCCCAGAGCTCTGTCCCAACCAAAAGGAAGCTATTGGTTGGTCCGACAGTTGTATGCTTCGCTATTCAAACCGCTCCATTTATGGAACCTTAGAAACTTTGCCTTTTTTCTCTTTAGTGAACCCATTTAACGTCTCAGCGTCAAGCCTGGATGGCTTCAACCAAGAGCTAAGGAAGTTGCTCAAAATCCTAAAAAGCGAAGCCGCAAAAGATGGTTCCCTTATTAAGTTTGCAAATGGAAACGCCAGCGCTCCAGGTGTCACAACAATATATGCACTTGTACAGTGTACGCCGGATTTGTCCCAGCTAGAGTGCAATGACTGCTTAGACGGTGCCTTTGTAGACATCCCAACCTGTTGTGATGGGAGGAAAGGTGGGAGAGTCATTAGACCCAGCTGTAACTTCAGATATGAAGACTTCCGTTTTTTTGACCCTAACGTGGTACTGCCATCGCCACCACTGCCATCAACAATATCTCCTCAGTCATCAACAATATCTCCTCAGCCAACAACAAATACTTCCGCAACTCCCGCAGGACTGAAGAATGATAAATCGCGATCTCGAACTGTCATCATTATTGTTGTGCCAATCGTTGTCTCTCTGGTGCTAATTATATCCCTCTACGTTTGCTTAAAAGCAAGGAATGAGAGAAAGGAAAAACTTGAAATACTCCCAGGTGAAGACAGTCAAGATACCGATGAAATTAGAACTGCGGATTCCTTGCAATTTGACTTTGACACCATCAGAGTTGCAACAAATAACTTTTCTGAAGCAAATAAACTTGGCCAAGGCGGATTTGGTTCTGTTTACAAGGGTACGCTTCTCAATGGAGAAGATATAGCAGTAAAAAGGCTTTCTATAAATTCTGGGCAAGGAAATTTAGAATTTAAAAATGAAGTGTTGTTAGTAGCCAAGCTTCAGCACAGGAATTTAGTGAGGCTTCTTGGTTTTTGCTTGGAAAGAAACGAAAGACTTCTTGTGTATGAGTTTGTTCCTAACGCAAGTCTTGATCACATCATATTTGACCGTATCAAGCGTGAACATTTAGATTGGGCTATGCGCTATAAAATCATAGTAGGCATTACTCGAGGGCTTCTTTATCTTCATGAAGATTCTCGCCTCAGGATCATACACCGTGATCTCAAAGCCAGTAACATCCTAATACATGCAAAAATGAATCCTAAAATATCAGACTTTGGCATGGCAAAGTTGTTCCAGCTTGATCAAACTCATGGCAATACGAGTCGAATTGTTGGGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

526

Amino Acids

58.33

Weight (kDa)

7.08

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 32 - 131 4.8e-25 Salt stress response/antifungal
Stress-antifung PF01657 157 - 245 7.3e-13 Salt stress response/antifungal
Pkinase PF00069 362 - 525 3.7e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 364 - 517 4e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 650
AccBSI CCGCTC 1 cut(s) 393
AccI GTMKAC 1 cut(s) 660
AccII CGCG 1 cut(s) 874
AciI CCGC 9 cut(s) 171, 219, 275, 391, 522, 840, 849, 1031, 1110
AclWI GGATC 1 cut(s) 1451
AcoI YGGCCR 1 cut(s) 1102
AcsI RAATTY 4 cut(s) 1180, 1195, 1202, 1246
AcuI CTGAAG 4 cut(s) 706, 878, 1107, 1220
AfaI GTAC 4 cut(s) 600, 607, 762, 1135
AfeI AGCGCT 1 cut(s) 568
AfiI CCNNNNNNNGG 1 cut(s) 757
AgsI TTSAA 5 cut(s) 284, 309, 387, 478, 983
AjnI CCWGG 3 cut(s) 465, 571, 991
AluBI AGCT 9 cut(s) 228, 294, 328, 350, 487, 625, 714, 1234, 1537
AluI AGCT 9 cut(s) 228, 294, 328, 350, 487, 625, 714, 1234, 1537
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 2 cut(s) 457, 919
AlwI GGATC 1 cut(s) 1451
Ama87I CYCGRG 1 cut(s) 1404
Aor51HI AGCGCT 1 cut(s) 568
AoxI GGCC 1 cut(s) 1102
ApeKI GCWGC 2 cut(s) 216, 294
ApoI RAATTY 4 cut(s) 1180, 1195, 1202, 1246
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
Asp700I GAANNNNTTC 1 cut(s) 1290
AspLEI GCGC 3 cut(s) 256, 569, 1380
AspS9I GGNCC 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 1007
AsuNHI GCTAGC 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 1404
AvaII GGWCC 1 cut(s) 360
AxyI CCTNAGG 1 cut(s) 1439
BalI TGGCCA 1 cut(s) 1104
BanI GGYRCC 1 cut(s) 650
BanII GRGCYC 1 cut(s) 330
BarI GAAGNNNNNNTAC 2 cut(s) 1218, 1250
BbsI GAAGAC 2 cut(s) 738, 1005
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 819
BbvI GCAGC 2 cut(s) 228, 281
BccI CCATC 8 cut(s) 28, 65, 464, 524, 674, 775, 790, 1067
BceAI ACGGC 2 cut(s) 202, 273
BciT130I CCWGG 3 cut(s) 467, 573, 993
BclI TGATCA 2 cut(s) 1327, 1540
BcoDI GTCTC 2 cut(s) 457, 919
BfaI CTAG 2 cut(s) 174, 626
BfoI RGCGCY 1 cut(s) 570
BglI GCCNNNNNGGC 1 cut(s) 471
BisI GCNGC 3 cut(s) 217, 295, 522
BlsI GCNGC 3 cut(s) 218, 296, 523
Bme1390I CCNGG 3 cut(s) 467, 573, 993
Bme18I GGWCC 1 cut(s) 360
BmeT110I CYCGRG 1 cut(s) 1404
BmgT120I GGNCC 1 cut(s) 360
BmiI GGNNCC 3 cut(s) 406, 535, 652
BmrFI CCNGG 3 cut(s) 467, 573, 993
BmtI GCTAGC 1 cut(s) 177
BpiI GAAGAC 2 cut(s) 738, 1005
BpmI CTGGAG 1 cut(s) 555
Bpu10I CCTNAGC 2 cut(s) 488, 819
Bsa29I ATCGAT 1 cut(s) 23
BsaAI YACGTR 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 991, 1105
BsaXI ACNNNNNCTCC 6 cut(s) 676, 706, 778, 799, 808, 829
Bsc4I CCNNNNNNNGG 1 cut(s) 757
Bse1I ACTGG 1 cut(s) 1467
Bse21I CCTNAGG 1 cut(s) 1439
Bse3DI GCAATG 1 cut(s) 640
BseBI CCWGG 3 cut(s) 467, 573, 993
BseCI ATCGAT 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 991, 1105
BseGI GGATG 4 cut(s) 60, 475, 663, 1473
BseLI CCNNNNNNNGG 1 cut(s) 757
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 4 cut(s) 468, 812, 833, 1453
BseNI ACTGG 1 cut(s) 1467
BseRI GAGGAG 3 cut(s) 151, 786, 807
BseXI GCAGC 2 cut(s) 228, 281
BseYI CCCAGC 2 cut(s) 621, 710
Bsh1236I CGCG 1 cut(s) 874
BshFI GGCC 1 cut(s) 1104
BshNI GGYRCC 1 cut(s) 650
BshVI ATCGAT 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 1404
BsiSI CCGG 1 cut(s) 611
BslFI GGGAC 2 cut(s) 318, 604
BslI CCNNNNNNNGG 1 cut(s) 757
BsmAI GTCTC 2 cut(s) 457, 919
BsmBI CGTCTC 1 cut(s) 457
BsmFI GGGAC 2 cut(s) 318, 604
BsmI GAATGC 2 cut(s) 98, 290
BsnI GGCC 1 cut(s) 1104
BsoBI CYCGRG 1 cut(s) 1404
Bsp1286I GDGCHC 1 cut(s) 330
Bsp1407I TGTACA 1 cut(s) 598
Bsp143I GATC 6 cut(s) 235, 875, 1327, 1443, 1456, 1540
Bsp68I TCGCGA 1 cut(s) 874
BspACI CCGC 9 cut(s) 171, 219, 275, 391, 522, 840, 849, 1031, 1110
BspANI GGCC 1 cut(s) 1104
BspCNI CTCAG 4 cut(s) 467, 811, 832, 1452
BspDI ATCGAT 1 cut(s) 23
BspFNI CGCG 1 cut(s) 874
BspHI TCATGA 1 cut(s) 1423
BspLI GGNNCC 3 cut(s) 406, 535, 652
BspOI GCTAGC 1 cut(s) 177
BspPI GGATC 1 cut(s) 1451
BspT107I GGYRCC 1 cut(s) 650
BsrBI CCGCTC 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 640
BsrGI TGTACA 1 cut(s) 598
BsrI ACTGG 1 cut(s) 1467
BssECI CCNNGG 2 cut(s) 991, 1105
BssMI GATC 6 cut(s) 235, 875, 1327, 1443, 1456, 1540
BssT1I CCWWGG 1 cut(s) 1105
Bst2UI CCWGG 3 cut(s) 467, 573, 993
Bst4CI ACNGT 8 cut(s) 182, 368, 603, 650, 886, 1004, 1346, 1454
Bst6I CTCTTC 1 cut(s) 171
BstAPI GCANNNNNTGC 1 cut(s) 639
BstAUI TGTACA 1 cut(s) 598
BstBAI YACGTR 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 175, 565
BstDEI CTNAG 8 cut(s) 409, 454, 488, 643, 798, 819, 1439, 1578
BstF5I GGATG 4 cut(s) 60, 475, 663, 1473
BstFNI CGCG 1 cut(s) 874
BstH2I RGCGCY 1 cut(s) 570
BstHHI GCGC 3 cut(s) 256, 569, 1380
BstKTI GATC 6 cut(s) 238, 878, 1330, 1446, 1459, 1543
BstMAI GTCTC 2 cut(s) 457, 919
BstMBI GATC 6 cut(s) 235, 875, 1327, 1443, 1456, 1540
BstMWI GCNNNNNNNGC 6 cut(s) 225, 294, 471, 639, 778, 848
BstNI CCWGG 3 cut(s) 467, 573, 993
BstNSI RCATGY 1 cut(s) 1487
BstSCI CCNGG 3 cut(s) 465, 571, 991
BstUI CGCG 1 cut(s) 874
BstV1I GCAGC 2 cut(s) 228, 281
BstV2I GAAGAC 2 cut(s) 738, 1005
Bsu15I ATCGAT 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 1439
BsuRI GGCC 1 cut(s) 1104
BsuTUI ATCGAT 1 cut(s) 23
BtgZI GCGATG 2 cut(s) 282, 753
BtsCI GGATG 4 cut(s) 60, 475, 663, 1473
BtsI GCAGTG 1 cut(s) 776
BtsIMutI CAGTG 2 cut(s) 608, 776
BtuMI TCGCGA 1 cut(s) 874
Cac8I GCNNGC 2 cut(s) 175, 565
CciI TCATGA 1 cut(s) 1423
CfoI GCGC 3 cut(s) 256, 569, 1380
Cfr13I GGNCC 1 cut(s) 360
ClaI ATCGAT 1 cut(s) 23
CseI GACGC 1 cut(s) 447
Csp6I GTAC 4 cut(s) 599, 606, 761, 1134
CviAII CATG 6 cut(s) 89, 245, 1424, 1484, 1519, 1550
CviQI GTAC 4 cut(s) 599, 606, 761, 1134
DdeI CTNAG 8 cut(s) 409, 454, 488, 643, 798, 819, 1439, 1578
DpnI GATC 6 cut(s) 237, 877, 1329, 1445, 1458, 1542
DpnII GATC 6 cut(s) 235, 875, 1327, 1443, 1456, 1540
DraI TTTAAA 1 cut(s) 1207
EaeI YGGCCR 1 cut(s) 1102
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
EciI GGCGGA 1 cut(s) 1125
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 1105
Eco24I GRGCYC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 360
Eco47III AGCGCT 1 cut(s) 568
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 4 cut(s) 706, 878, 1107, 1220
Eco81I CCTNAGG 1 cut(s) 1439
Eco88I CYCGRG 1 cut(s) 1404
EcoICRI GAGCTC 1 cut(s) 328
EcoRII CCWGG 3 cut(s) 465, 571, 991
EcoT14I CCWWGG 1 cut(s) 1105
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 1105
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 6 cut(s) 92, 248, 1427, 1487, 1522, 1553
FaqI GGGAC 2 cut(s) 318, 604
FatI CATG 6 cut(s) 88, 244, 1423, 1483, 1518, 1549
FauI CCCGC 3 cut(s) 178, 212, 856
FbaI TGATCA 2 cut(s) 1327, 1540
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 3 cut(s) 217, 295, 522
FokI GGATG 4 cut(s) 47, 482, 650, 1460
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 3 cut(s) 217, 295, 522
FspBI CTAG 2 cut(s) 174, 626
GlaI GCGC 3 cut(s) 255, 568, 1379
GluI GCNGC 3 cut(s) 217, 295, 522
GsaI CCCAGC 2 cut(s) 625, 714
GsuI CTGGAG 1 cut(s) 555
HaeII RGCGCY 1 cut(s) 570
HaeIII GGCC 1 cut(s) 1104
HapII CCGG 1 cut(s) 611
HgaI GACGC 1 cut(s) 447
HhaI GCGC 3 cut(s) 256, 569, 1380
Hin1II CATG 6 cut(s) 92, 248, 1427, 1487, 1522, 1553
Hin6I GCGC 3 cut(s) 254, 567, 1378
HinP1I GCGC 3 cut(s) 254, 567, 1378
HindIII AAGCTT 1 cut(s) 1232
HinfI GANTC 6 cut(s) 305, 699, 1034, 1430, 1495, 1561
HpaII CCGG 1 cut(s) 611
HphI GGTGA 1 cut(s) 1007
Hpy166II GTNNAC 5 cut(s) 439, 606, 661, 1126, 1358
Hpy188I TCNGA 5 cut(s) 364, 725, 1064, 1087, 1510
Hpy188III TCNNGA 7 cut(s) 52, 873, 879, 1007, 1325, 1424, 1441
Hpy8I GTNNAC 5 cut(s) 439, 606, 661, 1126, 1358
HpyCH4III ACNGT 8 cut(s) 182, 368, 603, 650, 886, 1004, 1346, 1454
HpyCH4IV ACGT 4 cut(s) 131, 450, 756, 942
HpyCH4V TGCA 7 cut(s) 261, 551, 593, 633, 1042, 1070, 1487
HpyF10VI GCNNNNNNNGC 6 cut(s) 225, 294, 471, 639, 778, 848
HpyF3I CTNAG 8 cut(s) 409, 454, 488, 643, 798, 819, 1439, 1578
HpySE526I ACGT 4 cut(s) 131, 450, 756, 942
Hsp92II CATG 6 cut(s) 92, 248, 1427, 1487, 1522, 1553
HspAI GCGC 3 cut(s) 254, 567, 1378
Ksp22I TGATCA 2 cut(s) 1327, 1540
Kzo9I GATC 6 cut(s) 235, 875, 1327, 1443, 1456, 1540
LmnI GCTCC 2 cut(s) 398, 574
Lsp1109I GCAGC 2 cut(s) 228, 281
MaeI CTAG 2 cut(s) 174, 626
MaeII ACGT 4 cut(s) 131, 450, 756, 942
MaeIII GTNAC 5 cut(s) 45, 182, 577, 716, 1469
MalI GATC 6 cut(s) 237, 877, 1329, 1445, 1458, 1542
MbiI CCGCTC 1 cut(s) 393
MboI GATC 6 cut(s) 235, 875, 1327, 1443, 1456, 1540
MboII GAAGA 9 cut(s) 149, 158, 198, 743, 871, 1010, 1163, 1412, 1439
MhlI GDGCHC 1 cut(s) 330
MlsI TGGCCA 1 cut(s) 1104
MluNI TGGCCA 1 cut(s) 1104
MlyI GAGTC 2 cut(s) 708, 1570
MmeI TCCRAC 1 cut(s) 387
Mox20I TGGCCA 1 cut(s) 1104
MroXI GAANNNNTTC 1 cut(s) 1290
MscI TGGCCA 1 cut(s) 1104
MseI TTAA 4 cut(s) 447, 543, 950, 1206
Msp20I TGGCCA 1 cut(s) 1104
MspA1I CMGCKG 3 cut(s) 219, 277, 714
MspI CCGG 1 cut(s) 611
MspR9I CCNGG 3 cut(s) 467, 573, 993
Mva1269I GAATGC 2 cut(s) 98, 290
MvaI CCWGG 3 cut(s) 467, 573, 993
MvnI CGCG 1 cut(s) 874
MwoI GCNNNNNNNGC 6 cut(s) 225, 294, 471, 639, 778, 848
NdeII GATC 6 cut(s) 235, 875, 1327, 1443, 1456, 1540
NheI GCTAGC 1 cut(s) 173
NlaIII CATG 6 cut(s) 92, 248, 1427, 1487, 1522, 1553
NlaIV GGNNCC 3 cut(s) 406, 535, 652
NmuCI GTSAC 1 cut(s) 577
NruI TCGCGA 1 cut(s) 874
NspI RCATGY 1 cut(s) 1487
PaeR7I CTCGAG 1 cut(s) 1404
PagI TCATGA 1 cut(s) 1423
PctI GAATGC 2 cut(s) 98, 290
PdmI GAANNNNTTC 1 cut(s) 1290
PfeI GAWTC 4 cut(s) 305, 1034, 1430, 1495
PkrI GCNGC 3 cut(s) 218, 296, 523
PleI GAGTC 2 cut(s) 707, 1569
PpsI GAGTC 2 cut(s) 707, 1569
Ppu21I YACGTR 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 465, 571, 991
PspFI CCCAGC 2 cut(s) 621, 710
PspGI CCWGG 3 cut(s) 465, 571, 991
PspN4I GGNNCC 3 cut(s) 406, 535, 652
PspPI GGNCC 1 cut(s) 360
PspXI VCTCGAGB 1 cut(s) 1404
PvuII CAGCTG 1 cut(s) 714
RruI TCGCGA 1 cut(s) 874
RsaI GTAC 4 cut(s) 600, 607, 762, 1135
RsaNI GTAC 4 cut(s) 599, 606, 761, 1134
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 4 cut(s) 447, 543, 950, 1206
SatI GCNGC 3 cut(s) 217, 295, 522
Sau3AI GATC 6 cut(s) 235, 875, 1327, 1443, 1456, 1540
Sau96I GGNCC 1 cut(s) 360
SchI GAGTC 2 cut(s) 708, 1570
ScrFI CCNGG 3 cut(s) 467, 573, 993
SduI GDGCHC 1 cut(s) 330
Sfr274I CTCGAG 1 cut(s) 1404
SinI GGWCC 1 cut(s) 360
SlaI CTCGAG 1 cut(s) 1404
SmlI CTYRAG 1 cut(s) 1404
SmoI CTYRAG 1 cut(s) 1404
SsiI CCGC 9 cut(s) 171, 219, 275, 391, 522, 840, 849, 1031, 1110
SspI AATATT 1 cut(s) 15
SspMI CTAG 2 cut(s) 174, 626
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 465, 571, 991
StyI CCWWGG 1 cut(s) 1105
TaaI ACNGT 8 cut(s) 182, 368, 603, 650, 886, 1004, 1346, 1454
TaiI ACGT 4 cut(s) 134, 453, 759, 945
TaqI TCGA 4 cut(s) 23, 880, 1405, 1564
TatI WGTACW 1 cut(s) 598
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 4 cut(s) 305, 1034, 1430, 1495
Tru1I TTAA 4 cut(s) 447, 543, 950, 1206
Tru9I TTAA 4 cut(s) 447, 543, 950, 1206
TscAI CASTG 2 cut(s) 608, 783
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 2 cut(s) 216, 294
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 9 cut(s) 105, 150, 233, 744, 1032, 1227, 1412, 1440, 1508
TspGWI ACGGA 1 cut(s) 728
TspRI CASTG 2 cut(s) 608, 783
VpaK11BI GGWCC 1 cut(s) 360
XapI RAATTY 4 cut(s) 1180, 1195, 1202, 1246
XceI RCATGY 1 cut(s) 1487
XcmI CCANNNNNNNNNTGG 1 cut(s) 1112
XhoI CTCGAG 1 cut(s) 1404
XmiI GTMKAC 1 cut(s) 660
XmnI GAANNNNTTC 1 cut(s) 1290
XspI CTAG 2 cut(s) 174, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.