Prupe.4G095100_v2.0.a1

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000812
Physical Location & Seq
Reverse (-)
4784283 .. 4785058
776 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G095100.1

Sequence Viewer

Length: 648 bp
ATGGCCTCCAAGTTTCTCTTGCTAGCATTGCTTGCCATAACTTCCTCCATTGCTTTTGCATCTGACCCGAGTTCTCTTCAGGATTTCTGCGTGGCTGACGCAATGAACTCAGTACTTGTGAATGGTCTTGTCTGCAAGGATCCCAAGCTTGCTGAAGCCAATGACTTCTTCTTCAGTGGGCTTCACCTAGCAGGCAACACATCAAATGCAGTTGGTTCGCGTGTGACCCCTGTCAACGTAGCCCAAATTGCAGGGCTTAACGCTCTTGGTATCTCCATTGCGCGTATCGACTATGCGCCATGGGGTATTAACCCTCCCCACACACATCCTCGAGCTAGTGAAATCTTGACAGTCCTGGAAGGGAGCCTCAAAGTGGGTTTTGTCACTTCCAACCCTGAAAACCGTCTCATCTCCAAGGTACTTGAGAAGGGTGATGTGTTTGTGTTCCCTGTCGGTCTCGTGCATTTCCAACAAAATGTGGGATATGGTAATGCAGTTGCCATTGCAGCTCTCAGCAGCCAAAATCCAGGTGTCATTACCATTGCAAATGCAGTATTTGGATCAAAGCCCGACATCTCTGCTGATATTCTTGCAAAGGCTTTCCAAGTGGAGAAGAACACAGTCTACAATTTGCAGTCGAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

216

Amino Acids

22.64

Weight (kDa)

6.4

Isoelectric Point (pI)

18.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 624
AccII CGCG 2 cut(s) 220, 283
AclWI GGATC 3 cut(s) 134, 147, 568
AcuI CTGAAG 3 cut(s) 62, 157, 174
AfaI GTAC 2 cut(s) 114, 420
AfiI CCNNNNNNNGG 1 cut(s) 373
AjnI CCWGG 2 cut(s) 354, 526
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AluBI AGCT 3 cut(s) 148, 335, 509
AluI AGCT 3 cut(s) 148, 335, 509
Alw26I GTCTC 2 cut(s) 410, 461
AlwI GGATC 3 cut(s) 134, 147, 568
Ama87I CYCGRG 2 cut(s) 67, 330
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 506, 516
AspLEI GCGC 2 cut(s) 283, 298
AsuHPI GGTGA 2 cut(s) 176, 443
AsuNHI GCTAGC 1 cut(s) 22
AvaI CYCGRG 2 cut(s) 67, 330
BamHI GGATCC 1 cut(s) 139
BauI CACGAG 1 cut(s) 458
BbvI GCAGC 2 cut(s) 518, 528
BcgI CGANNNNNNTGC 4 cut(s) 198, 232, 560, 594
BciT130I CCWGG 2 cut(s) 356, 528
BcoDI GTCTC 2 cut(s) 410, 461
BfaI CTAG 3 cut(s) 23, 188, 336
BisI GCNGC 2 cut(s) 507, 517
BlsI GCNGC 2 cut(s) 508, 518
BmcAI AGTACT 1 cut(s) 114
Bme1390I CCNGG 2 cut(s) 356, 528
BmeT110I CYCGRG 2 cut(s) 67, 330
BmiI GGNNCC 2 cut(s) 141, 365
BmrFI CCNGG 2 cut(s) 356, 528
BmsI GCATC 1 cut(s) 68
BmtI GCTAGC 1 cut(s) 26
BoxI GACNNNNGTC 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 2 cut(s) 299, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse3DI GCAATG 6 cut(s) 26, 48, 108, 276, 501, 540
BseBI CCWGG 2 cut(s) 356, 528
BseDI CCNNGG 2 cut(s) 299, 414
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMI GCAATG 6 cut(s) 26, 48, 108, 276, 501, 540
BseMII CTCAG 2 cut(s) 123, 526
BseXI GCAGC 2 cut(s) 518, 528
Bsh1236I CGCG 2 cut(s) 220, 283
BshFI GGCC 1 cut(s) 5
BsiHKCI CYCGRG 2 cut(s) 67, 330
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 2 cut(s) 410, 461
BsmBI CGTCTC 1 cut(s) 410
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 2 cut(s) 67, 330
Bsp143I GATC 2 cut(s) 139, 560
Bsp19I CCATGG 1 cut(s) 299
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 122, 525
BspFNI CGCG 2 cut(s) 220, 283
BspLI GGNNCC 2 cut(s) 141, 365
BspOI GCTAGC 1 cut(s) 26
BspPI GGATC 3 cut(s) 134, 147, 568
BspTNI GGTCTC 1 cut(s) 461
BsrDI GCAATG 6 cut(s) 26, 48, 108, 276, 501, 540
BssECI CCNNGG 2 cut(s) 299, 414
BssMI GATC 2 cut(s) 139, 560
BssSI CACGAG 1 cut(s) 458
BssT1I CCWWGG 2 cut(s) 299, 414
Bst2BI CACGAG 1 cut(s) 458
Bst2UI CCWGG 2 cut(s) 356, 528
Bst4CI ACNGT 3 cut(s) 352, 404, 622
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 4 cut(s) 24, 33, 150, 193
BstDEI CTNAG 2 cut(s) 109, 512
BstDSI CCRYGG 1 cut(s) 299
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 2 cut(s) 220, 283
BstHHI GCGC 2 cut(s) 283, 298
BstKTI GATC 2 cut(s) 142, 563
BstMAI GTCTC 2 cut(s) 410, 461
BstMBI GATC 2 cut(s) 139, 560
BstMWI GCNNNNNNNGC 4 cut(s) 28, 32, 248, 506
BstNI CCWGG 2 cut(s) 356, 528
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 354, 526
BstUI CGCG 2 cut(s) 220, 283
BstV1I GCAGC 2 cut(s) 518, 528
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 299
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 4 cut(s) 24, 33, 150, 193
CfoI GCGC 2 cut(s) 283, 298
CseI GACGC 1 cut(s) 107
Csp6I GTAC 2 cut(s) 113, 419
CviAII CATG 1 cut(s) 300
CviQI GTAC 2 cut(s) 113, 419
DdeI CTNAG 2 cut(s) 109, 512
DpnI GATC 2 cut(s) 141, 562
DpnII GATC 2 cut(s) 139, 560
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 299, 414
Eco31I GGTCTC 1 cut(s) 461
Eco57I CTGAAG 3 cut(s) 62, 157, 174
Eco88I CYCGRG 2 cut(s) 67, 330
EcoRII CCWGG 2 cut(s) 354, 526
EcoT14I CCWWGG 2 cut(s) 299, 414
ErhI CCWWGG 2 cut(s) 299, 414
Esp3I CGTCTC 1 cut(s) 410
FaeI CATG 1 cut(s) 303
FaiI YATR 4 cut(s) 38, 294, 301, 486
FalI AAGNNNNNCTT 1 cut(s) 34
FatI CATG 1 cut(s) 299
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 2 cut(s) 507, 517
FokI GGATG 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 507, 517
FspBI CTAG 3 cut(s) 23, 188, 336
GlaI GCGC 2 cut(s) 282, 297
GluI GCNGC 2 cut(s) 507, 517
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 283, 298
Hin1II CATG 1 cut(s) 303
Hin6I GCGC 2 cut(s) 281, 296
HinP1I GCGC 2 cut(s) 281, 296
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 146
HphI GGTGA 2 cut(s) 176, 443
Hpy166II GTNNAC 2 cut(s) 235, 625
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 2 cut(s) 80, 346
Hpy8I GTNNAC 2 cut(s) 235, 625
HpyAV CCTTC 2 cut(s) 353, 421
HpyCH4III ACNGT 3 cut(s) 352, 404, 622
HpyCH4IV ACGT 1 cut(s) 237
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 32, 248, 506
HpyF3I CTNAG 2 cut(s) 109, 512
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 1 cut(s) 303
HspAI GCGC 2 cut(s) 281, 296
Kzo9I GATC 2 cut(s) 139, 560
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 2 cut(s) 518, 528
LweI GCATC 1 cut(s) 68
MaeI CTAG 3 cut(s) 23, 188, 336
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 223, 382
MalI GATC 2 cut(s) 141, 562
MboI GATC 2 cut(s) 139, 560
MboII GAAGA 4 cut(s) 68, 160, 163, 625
MflI RGATCY 1 cut(s) 139
MluCI AATT 2 cut(s) 246, 628
MmeI TCCRAC 2 cut(s) 414, 493
MnlI CCTC 5 cut(s) 16, 55, 324, 339, 377
MseI TTAA 2 cut(s) 258, 309
MspR9I CCNGG 2 cut(s) 356, 528
MvaI CCWGG 2 cut(s) 356, 528
MvnI CGCG 2 cut(s) 220, 283
MwoI GCNNNNNNNGC 4 cut(s) 28, 32, 248, 506
NcoI CCATGG 1 cut(s) 299
NdeII GATC 2 cut(s) 139, 560
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 1 cut(s) 303
NlaIV GGNNCC 2 cut(s) 141, 365
NmuCI GTSAC 2 cut(s) 223, 382
PaeR7I CTCGAG 1 cut(s) 330
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 2 cut(s) 508, 518
PshAI GACNNNNGTC 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 354, 526
PspGI CCWGG 2 cut(s) 354, 526
PspN4I GGNNCC 2 cut(s) 141, 365
PspXI VCTCGAGB 1 cut(s) 330
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 2 cut(s) 114, 420
RsaNI GTAC 2 cut(s) 113, 419
SaqAI TTAA 2 cut(s) 258, 309
SatI GCNGC 2 cut(s) 507, 517
Sau3AI GATC 2 cut(s) 139, 560
ScaI AGTACT 1 cut(s) 114
ScrFI CCNGG 2 cut(s) 356, 528
SetI ASST 7 cut(s) 150, 189, 240, 337, 420, 511, 532
SfaNI GCATC 1 cut(s) 68
Sfr274I CTCGAG 1 cut(s) 330
SlaI CTCGAG 1 cut(s) 330
SmlI CTYRAG 2 cut(s) 330, 422
SmoI CTYRAG 2 cut(s) 330, 422
Sse9I AATT 2 cut(s) 246, 628
SspMI CTAG 3 cut(s) 23, 188, 336
StyD4I CCNGG 2 cut(s) 354, 526
StyI CCWWGG 2 cut(s) 299, 414
TaaI ACNGT 3 cut(s) 352, 404, 622
TaiI ACGT 1 cut(s) 240
TaqI TCGA 3 cut(s) 288, 331, 638
TaqII GACCGA 1 cut(s) 443
TasI AATT 2 cut(s) 246, 628
TatI WGTACW 1 cut(s) 112
Tru1I TTAA 2 cut(s) 258, 309
Tru9I TTAA 2 cut(s) 258, 309
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 2 cut(s) 223, 382
TseI GCWGC 2 cut(s) 506, 516
Tsp45I GTSAC 2 cut(s) 223, 382
TspDTI ATGAA 1 cut(s) 119
TspRI CASTG 1 cut(s) 181
XhoI CTCGAG 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 624
XspI CTAG 3 cut(s) 23, 188, 336
ZrmI AGTACT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.