Prupe.4G094900_v2.0.a1

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000812
Physical Location & Seq
Reverse (-)
4778933 .. 4780438
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G094900.1

Sequence Viewer

Length: 648 bp
ATGGCCTCTCAGCTTCTGTTGTTGGCTTTCCTTGCCATAACTTCTTCCATTGCTTTGGCATCTGACCCAAGTTCTCTTCAAGATTTCTGCGTGGCTGATCCGAAAACCTCAGTACTTGTGAATGGTGTTGTCTGCAAGGATTCCAAACTTGTTGATGTCAATGACTTCTTCTTCAGTGGGCTTCACCTAGCAGGAAACACATCAAACGCAGTTGGCTCAAGCGTGACCTCTGTTAATGTAGCTCAAATAGCAGGACTTAATACTCTTAGCATCTCCATTGCGCGAATCGACTACGCACCATGGGGAATTAATCCTCCCCACACGCATCCTCGAGCAAGCGAAATCTTAACAGTCCTGGAAGGGAGCCTCAAAGTGGGTTTTGTCACCTCCAACCCTGAAAACCGCCACATCACCAAGGTGCTTCAGAAGGGTGATGTGTTTGTGTTCCCAGTAGGCCTTGTGCATTACCAACAAAATGTCGGATATGGTAATGCAGTTGCTATCGCAGCTCTCAGCAGCCAAAATCCAGGTGTCATTACCATAGCAAATGCGGTATTTGGATCAAAGCCGGACATCTCTGCTGATATTGTTGCAAAGGCTTTCCAAGTGGATAAGAATGCAGTCTACAATTTCCAGTCCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

216

Amino Acids

22.64

Weight (kDa)

6.49

Isoelectric Point (pI)

21.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 624
AccII CGCG 1 cut(s) 283
AciI CCGC 2 cut(s) 403, 551
AclWI GGATC 2 cut(s) 92, 568
AcuI CTGAAG 2 cut(s) 157, 407
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 1 cut(s) 373
AgsI TTSAA 1 cut(s) 80
AjnI CCWGG 2 cut(s) 354, 526
AleI CACNNNNGTG 1 cut(s) 416
AluBI AGCT 3 cut(s) 13, 242, 509
AluI AGCT 3 cut(s) 13, 242, 509
AlwI GGATC 2 cut(s) 92, 568
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 2 cut(s) 3, 454
ApeKI GCWGC 2 cut(s) 506, 516
AseI ATTAAT 1 cut(s) 309
AspLEI GCGC 1 cut(s) 283
AsuHPI GGTGA 4 cut(s) 176, 376, 403, 443
AvaI CYCGRG 1 cut(s) 330
BbvI GCAGC 2 cut(s) 518, 528
BciT130I CCWGG 2 cut(s) 356, 528
BfaI CTAG 1 cut(s) 188
BisI GCNGC 2 cut(s) 507, 517
BlsI GCNGC 2 cut(s) 508, 518
BmcAI AGTACT 1 cut(s) 114
Bme1390I CCNGG 2 cut(s) 356, 528
BmeT110I CYCGRG 1 cut(s) 330
BmiI GGNNCC 1 cut(s) 365
BmrFI CCNGG 2 cut(s) 356, 528
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 3 cut(s) 68, 279, 334
BmuI ACTGGG 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 299, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse1I ACTGG 2 cut(s) 449, 634
Bse3DI GCAATG 2 cut(s) 48, 276
BseBI CCWGG 2 cut(s) 356, 528
BseDI CCNNGG 2 cut(s) 299, 414
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMI GCAATG 2 cut(s) 48, 276
BseMII CTCAG 3 cut(s) 23, 123, 526
BseNI ACTGG 2 cut(s) 449, 634
BseXI GCAGC 2 cut(s) 518, 528
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 2 cut(s) 5, 456
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 1 cut(s) 569
BslI CCNNNNNNNGG 1 cut(s) 373
BsmI GAATGC 1 cut(s) 622
BsnI GGCC 2 cut(s) 5, 456
BsoBI CYCGRG 1 cut(s) 330
Bsp143I GATC 2 cut(s) 97, 560
Bsp19I CCATGG 1 cut(s) 299
BspACI CCGC 2 cut(s) 403, 551
BspANI GGCC 2 cut(s) 5, 456
BspCNI CTCAG 3 cut(s) 22, 122, 525
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 365
BspPI GGATC 2 cut(s) 92, 568
BsrDI GCAATG 2 cut(s) 48, 276
BsrI ACTGG 2 cut(s) 449, 634
BssECI CCNNGG 2 cut(s) 299, 414
BssMI GATC 2 cut(s) 97, 560
BssT1I CCWWGG 2 cut(s) 299, 414
Bst2UI CCWGG 2 cut(s) 356, 528
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 4 cut(s) 9, 109, 266, 512
BstDSI CCRYGG 1 cut(s) 299
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 2 cut(s) 100, 563
BstMBI GATC 2 cut(s) 97, 560
BstMWI GCNNNNNNNGC 3 cut(s) 32, 248, 506
BstNI CCWGG 2 cut(s) 356, 528
BstSCI CCNGG 2 cut(s) 354, 526
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 2 cut(s) 518, 528
BstXI CCANNNNNNTGG 1 cut(s) 55
BsuRI GGCC 2 cut(s) 5, 456
BtgI CCRYGG 1 cut(s) 299
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 337
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 113
CviAII CATG 1 cut(s) 300
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 4 cut(s) 9, 109, 266, 512
DpnI GATC 2 cut(s) 99, 562
DpnII GATC 2 cut(s) 97, 560
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 299, 414
Eco147I AGGCCT 1 cut(s) 456
Eco57I CTGAAG 2 cut(s) 157, 407
Eco88I CYCGRG 1 cut(s) 330
EcoRII CCWGG 2 cut(s) 354, 526
EcoT14I CCWWGG 2 cut(s) 299, 414
ErhI CCWWGG 2 cut(s) 299, 414
FaeI CATG 1 cut(s) 303
FaiI YATR 4 cut(s) 38, 301, 486, 542
FatI CATG 1 cut(s) 299
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 2 cut(s) 507, 517
FokI GGATG 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 507, 517
FspBI CTAG 1 cut(s) 188
GlaI GCGC 1 cut(s) 282
GluI GCNGC 2 cut(s) 507, 517
HaeIII GGCC 2 cut(s) 5, 456
HapII CCGG 1 cut(s) 569
HhaI GCGC 1 cut(s) 283
Hin1II CATG 1 cut(s) 303
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 2 cut(s) 140, 285
HpaII CCGG 1 cut(s) 569
HphI GGTGA 4 cut(s) 176, 376, 403, 443
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 4 cut(s) 64, 102, 426, 482
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 625
HpyAV CCTTC 2 cut(s) 353, 421
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 5 cut(s) 135, 463, 494, 593, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 248, 506
HpyF3I CTNAG 4 cut(s) 9, 109, 266, 512
Hsp92II CATG 1 cut(s) 303
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 2 cut(s) 97, 560
LmnI GCTCC 1 cut(s) 363
LpnPI CCDG 9 cut(s) 177, 237, 341, 368, 408, 462, 513, 540, 582
Lsp1109I GCAGC 2 cut(s) 518, 528
LweI GCATC 3 cut(s) 68, 279, 334
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 2 cut(s) 223, 382
MalI GATC 2 cut(s) 99, 562
MboI GATC 2 cut(s) 97, 560
MboII GAAGA 4 cut(s) 36, 68, 160, 163
MluCI AATT 2 cut(s) 306, 628
MmeI TCCRAC 2 cut(s) 414, 460
MnlI CCTC 7 cut(s) 16, 118, 238, 324, 339, 377, 397
MseI TTAA 4 cut(s) 234, 258, 309, 347
MslI CAYNNNNRTG 1 cut(s) 416
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 2 cut(s) 356, 528
Mva1269I GAATGC 1 cut(s) 622
MvaI CCWGG 2 cut(s) 356, 528
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 3 cut(s) 32, 248, 506
NcoI CCATGG 1 cut(s) 299
NdeII GATC 2 cut(s) 97, 560
NlaIII CATG 1 cut(s) 303
NlaIV GGNNCC 1 cut(s) 365
NmuCI GTSAC 2 cut(s) 223, 382
OliI CACNNNNGTG 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 330
PceI AGGCCT 1 cut(s) 456
PctI GAATGC 1 cut(s) 622
PfeI GAWTC 2 cut(s) 140, 285
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 2 cut(s) 508, 518
PshBI ATTAAT 1 cut(s) 309
Psp6I CCWGG 2 cut(s) 354, 526
PspGI CCWGG 2 cut(s) 354, 526
PspN4I GGNNCC 1 cut(s) 365
PspXI VCTCGAGB 1 cut(s) 330
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
RseI CAYNNNNRTG 1 cut(s) 416
SaqAI TTAA 4 cut(s) 234, 258, 309, 347
SatI GCNGC 2 cut(s) 507, 517
Sau3AI GATC 2 cut(s) 97, 560
ScaI AGTACT 1 cut(s) 114
ScrFI CCNGG 2 cut(s) 356, 528
SetI ASST 9 cut(s) 15, 110, 189, 230, 244, 389, 420, 511, 532
SfaNI GCATC 3 cut(s) 68, 279, 334
Sfr274I CTCGAG 1 cut(s) 330
SlaI CTCGAG 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 217, 330
SmoI CTYRAG 2 cut(s) 217, 330
Sse9I AATT 2 cut(s) 306, 628
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 2 cut(s) 403, 551
SspMI CTAG 1 cut(s) 188
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 2 cut(s) 354, 526
StyI CCWWGG 2 cut(s) 299, 414
TaaI ACNGT 1 cut(s) 352
TaqI TCGA 2 cut(s) 288, 331
TasI AATT 2 cut(s) 306, 628
TatI WGTACW 1 cut(s) 112
TfiI GAWTC 2 cut(s) 140, 285
Tru1I TTAA 4 cut(s) 234, 258, 309, 347
Tru9I TTAA 4 cut(s) 234, 258, 309, 347
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 2 cut(s) 223, 382
TseI GCWGC 2 cut(s) 506, 516
Tsp45I GTSAC 2 cut(s) 223, 382
TspRI CASTG 1 cut(s) 181
VspI ATTAAT 1 cut(s) 309
XhoI CTCGAG 1 cut(s) 330
XmiI GTMKAC 1 cut(s) 624
XspI CTAG 1 cut(s) 188
ZrmI AGTACT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.