pycom05g25250

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000812
Physical Location & Seq
Forward (+)
26693558 .. 26694139
582 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25250.1

Sequence Viewer

Length: 582 bp
ATGTATACTGCTAATGTTAATGTACTTTTTCTCATCATGGTTTCAGTACTGGTTAATGGTCGTGTTTGCAAGGACCCTAAACTAGTTGAAGCCAACGAGTTCTTTTTCAGTGGGCTTCACCTAGCAAGCAACACATCAAACGCAGTTGGTTCACGTGTGACTTCTGTTGGTGTAGCTCAAATTGGAGGACTTAATGCCCTTGGCATCACCACTGCGCGTATTGATTTTGCCACATGGGGTCTTAACCCTCCACATACACACCCTAGAGCTACTGAGATTTTGACGGTCCTGGAAGGGAGCCTCAAAGTTGGTTTTGTCACCTCGAACACTGAAAACCGCCTCATCACCAAGGTACTTCGGAAGGGTGATGTTTTTGTGTTCCCAGTCGGTCTTGTGCATTTCCAGCAGAATGTTGGAAATGGTAATGCAGTTGCCCTTGTGGCTCTCAGCAGCCAAAATCCAGGTGTCATTACCATTGCAAATGCAGTATTTGGATCCAATCCTGATATCCCTGCTGATATTCTTGCAAAGGCTTTCCAAATGGGTACGAACGCGATCTATAGCCTTCAGTCCAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

194

Amino Acids

20.45

Weight (kDa)

9.25

Isoelectric Point (pI)

14.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 39 - 184 1.2e-47 Cupin
Cupin_2 PF07883 82 - 147 1.1e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 217, 554
AciI CCGC 1 cut(s) 337
AclWI GGATC 2 cut(s) 489, 502
AcuI CTGAAG 1 cut(s) 551
AcvI CACGTG 1 cut(s) 155
AfaI GTAC 4 cut(s) 24, 48, 354, 547
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 1 cut(s) 89
AhlI ACTAGT 1 cut(s) 82
AjnI CCWGG 2 cut(s) 288, 460
AluBI AGCT 2 cut(s) 176, 269
AluI AGCT 2 cut(s) 176, 269
AlwI GGATC 2 cut(s) 489, 502
ApeKI GCWGC 1 cut(s) 450
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 73, 286
AsuHPI GGTGA 5 cut(s) 110, 199, 310, 337, 377
AvaII GGWCC 2 cut(s) 73, 286
BamHI GGATCC 1 cut(s) 494
BbrPI CACGTG 1 cut(s) 155
BbvI GCAGC 1 cut(s) 462
BciT130I CCWGG 2 cut(s) 290, 462
BcuI ACTAGT 1 cut(s) 82
BfaI CTAG 4 cut(s) 83, 122, 264, 580
BfmI CTRYAG 1 cut(s) 559
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 1 cut(s) 451
BlsI GCNGC 1 cut(s) 452
BmcAI AGTACT 1 cut(s) 48
Bme1390I CCNGG 2 cut(s) 290, 462
Bme18I GGWCC 2 cut(s) 73, 286
BmgT120I GGNCC 2 cut(s) 73, 286
BmiI GGNNCC 3 cut(s) 75, 299, 496
BmrFI CCNGG 2 cut(s) 290, 462
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 377
BsaAI YACGTR 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 199, 348
Bse1I ACTGG 2 cut(s) 54, 383
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 2 cut(s) 290, 462
BseDI CCNNGG 2 cut(s) 199, 348
BseMI GCAATG 1 cut(s) 474
BseMII CTCAG 2 cut(s) 264, 460
BseNI ACTGG 2 cut(s) 54, 383
BseXI GCAGC 1 cut(s) 462
Bsh1236I CGCG 2 cut(s) 217, 554
Bsp143I GATC 2 cut(s) 494, 555
BspACI CCGC 1 cut(s) 337
BspCNI CTCAG 2 cut(s) 265, 459
BspFNI CGCG 2 cut(s) 217, 554
BspLI GGNNCC 3 cut(s) 75, 299, 496
BspPI GGATC 2 cut(s) 489, 502
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 2 cut(s) 54, 383
BssECI CCNNGG 2 cut(s) 199, 348
BssMI GATC 2 cut(s) 494, 555
BssNAI GTATAC 1 cut(s) 6
BssT1I CCWWGG 2 cut(s) 199, 348
Bst1107I GTATAC 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 290, 462
Bst4CI ACNGT 1 cut(s) 286
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 2 cut(s) 273, 446
BstFNI CGCG 2 cut(s) 217, 554
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 2 cut(s) 497, 558
BstMBI GATC 2 cut(s) 494, 555
BstMWI GCNNNNNNNGC 2 cut(s) 403, 440
BstNI CCWGG 2 cut(s) 290, 462
BstSCI CCNGG 2 cut(s) 288, 460
BstSFI CTRYAG 1 cut(s) 559
BstUI CGCG 2 cut(s) 217, 554
BstV1I GCAGC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 494
BstYI RGATCY 1 cut(s) 494
BstZ17I GTATAC 1 cut(s) 6
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 3 cut(s) 115, 210, 327
Cac8I GCNNGC 1 cut(s) 127
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 73, 286
Csp6I GTAC 4 cut(s) 23, 47, 353, 546
CviAII CATG 2 cut(s) 37, 234
CviJI RGCY 9 cut(s) 92, 115, 176, 269, 300, 443, 453, 533, 564
CviKI_1 RGCY 9 cut(s) 92, 115, 176, 269, 300, 443, 453, 533, 564
CviQI GTAC 4 cut(s) 23, 47, 353, 546
DdeI CTNAG 2 cut(s) 273, 446
DpnI GATC 2 cut(s) 496, 557
DpnII GATC 2 cut(s) 494, 555
Eco130I CCWWGG 2 cut(s) 199, 348
Eco32I GATATC 1 cut(s) 508
Eco47I GGWCC 2 cut(s) 73, 286
Eco57I CTGAAG 1 cut(s) 551
Eco72I CACGTG 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 2 cut(s) 288, 460
EcoRV GATATC 1 cut(s) 508
EcoT14I CCWWGG 2 cut(s) 199, 348
ErhI CCWWGG 2 cut(s) 199, 348
FaeI CATG 2 cut(s) 40, 237
FaiI YATR 5 cut(s) 6, 38, 235, 255, 561
FatI CATG 2 cut(s) 36, 233
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 451
Fsp4HI GCNGC 1 cut(s) 451
FspBI CTAG 4 cut(s) 83, 122, 264, 580
GlaI GCGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 451
HhaI GCGC 1 cut(s) 217
Hin1II CATG 2 cut(s) 40, 237
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HphI GGTGA 5 cut(s) 110, 199, 310, 337, 377
Hpy166II GTNNAC 2 cut(s) 6, 152
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 1 cut(s) 503
Hpy8I GTNNAC 2 cut(s) 6, 152
HpyAV CCTTC 3 cut(s) 287, 355, 575
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 6 cut(s) 69, 397, 428, 479, 485, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 403, 440
HpyF3I CTNAG 2 cut(s) 273, 446
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 2 cut(s) 40, 237
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 2 cut(s) 494, 555
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 9 cut(s) 35, 275, 302, 396, 416, 447, 474, 516, 525
Lsp1109I GCAGC 1 cut(s) 462
LweI GCATC 1 cut(s) 213
MaeI CTAG 4 cut(s) 83, 122, 264, 580
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 2 cut(s) 157, 316
MalI GATC 2 cut(s) 496, 557
MboI GATC 2 cut(s) 494, 555
MflI RGATCY 1 cut(s) 494
MluCI AATT 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 394
MnlI CCTC 5 cut(s) 179, 258, 311, 331, 350
MseI TTAA 4 cut(s) 18, 54, 192, 243
MspR9I CCNGG 2 cut(s) 290, 462
MvaI CCWGG 2 cut(s) 290, 462
MvnI CGCG 2 cut(s) 217, 554
MwoI GCNNNNNNNGC 2 cut(s) 403, 440
NdeII GATC 2 cut(s) 494, 555
NlaIII CATG 2 cut(s) 40, 237
NlaIV GGNNCC 3 cut(s) 75, 299, 496
NmuCI GTSAC 2 cut(s) 157, 316
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 1 cut(s) 452
PmaCI CACGTG 1 cut(s) 155
PmlI CACGTG 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 2 cut(s) 288, 460
PspCI CACGTG 1 cut(s) 155
PspGI CCWGG 2 cut(s) 288, 460
PspN4I GGNNCC 3 cut(s) 75, 299, 496
PspPI GGNCC 2 cut(s) 73, 286
PspPPI RGGWCCY 1 cut(s) 73
PsuI RGATCY 1 cut(s) 494
RsaI GTAC 4 cut(s) 24, 48, 354, 547
RsaNI GTAC 4 cut(s) 23, 47, 353, 546
SaqAI TTAA 4 cut(s) 18, 54, 192, 243
SatI GCNGC 1 cut(s) 451
Sau3AI GATC 2 cut(s) 494, 555
Sau96I GGNCC 2 cut(s) 73, 286
ScaI AGTACT 1 cut(s) 48
ScrFI CCNGG 2 cut(s) 290, 462
SetI ASST 7 cut(s) 123, 157, 178, 271, 323, 354, 466
SfaNI GCATC 1 cut(s) 213
SfcI CTRYAG 1 cut(s) 559
SinI GGWCC 2 cut(s) 73, 286
SpeI ACTAGT 1 cut(s) 82
Sse9I AATT 1 cut(s) 180
SsiI CCGC 1 cut(s) 337
SspMI CTAG 4 cut(s) 83, 122, 264, 580
StyD4I CCNGG 2 cut(s) 288, 460
StyI CCWWGG 2 cut(s) 199, 348
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 157
TaqI TCGA 1 cut(s) 323
TaqII GACCGA 1 cut(s) 377
TasI AATT 1 cut(s) 180
TatI WGTACW 2 cut(s) 22, 46
Tru1I TTAA 4 cut(s) 18, 54, 192, 243
Tru9I TTAA 4 cut(s) 18, 54, 192, 243
TscAI CASTG 3 cut(s) 115, 217, 334
TseFI GTSAC 2 cut(s) 157, 316
TseI GCWGC 1 cut(s) 450
Tsp45I GTSAC 2 cut(s) 157, 316
TspRI CASTG 3 cut(s) 115, 217, 334
VpaK11BI GGWCC 2 cut(s) 73, 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 4 cut(s) 83, 122, 264, 580
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.