Rh5CG140400

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000812
Physical Location & Seq
Reverse (-)
12223583 .. 12224892
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG140400.1

Sequence Viewer

Length: 648 bp
ATGGCCTCTCAGCTTCTATTGTTGGCATTCCTTGCCATAACTTGTTCCGTTGCTTTTGCTTCTGACCCAAGCTCTCTTCAGGACTTTTGTGTGGCTGAGTCTACAAGCTCAGTACCAGTGAATGGACATGTGTGCAAGGACCCCAAGCTTGTCGAAGCCAATGACTTCTTCCTCAGTGGACTGCACCTAGCAGGCAACACATCAAACCCTGCTGGTTCGAAGGTGACCCCTGCTAATGTAGCCCAGATTCCAGGACTCAACACTTTTGGTATCTCCCTAGTTCGTATCGACTATGCACCAGGGGGTATAAATGCTCCACACACTCATCCTAGAGCTACTGAAATCTTGACAGTGGTTGAAGGTAGCCTAGAAGTGGGTTTTGTCACCTCCAACCCTGAAAACCGTCTCATCTCCAAGGTGCTTCAGAAGGGTGATGTGTTTGTGTTCCCAGTAGGACTAGCGCATTTCCAGCACAATGTTGGAAAAGGTAATGCAGTCGCCATTGCAGCTCTCAGCAGCCAAAATCCAGGTGCTATTACCCTTGCCAACGCAGTGTTCGGGTCAAAGCCAGACATCTCTGCCGATGTTCTTGCAAAGGCTTTCCAAGTGGATAAGAACATAGTCTTCGATTTTCAAGCCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

215

Amino Acids

22.43

Weight (kDa)

6.03

Isoelectric Point (pI)

22.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 61 - 207 9e-53 Cupin
Cupin_2 PF07883 96 - 168 9.6e-10 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 122
AccI GTMKAC 1 cut(s) 101
AcuI CTGAAG 2 cut(s) 62, 407
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 2 cut(s) 122, 373
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 2 cut(s) 359, 635
AjnI CCWGG 3 cut(s) 250, 298, 526
AjuI GAANNNNNNNTTGG 4 cut(s) 152, 184, 539, 571
AluBI AGCT 6 cut(s) 13, 72, 108, 148, 335, 509
AluI AGCT 6 cut(s) 13, 72, 108, 148, 335, 509
Alw26I GTCTC 1 cut(s) 410
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 506, 516
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 3 cut(s) 235, 376, 443
AsuII TTCGAA 1 cut(s) 218
AvaII GGWCC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 616
BbvI GCAGC 2 cut(s) 518, 528
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 3 cut(s) 252, 300, 528
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 5 cut(s) 188, 278, 330, 368, 458
BisI GCNGC 2 cut(s) 507, 517
BlsI GCNGC 2 cut(s) 508, 518
Bme1390I CCNGG 3 cut(s) 252, 300, 528
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 3 cut(s) 252, 300, 528
BmrI ACTGGG 1 cut(s) 443
BmuI ACTGGG 1 cut(s) 443
BpiI GAAGAC 1 cut(s) 616
Bpu14I TTCGAA 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 299, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 373
Bse1I ACTGG 2 cut(s) 116, 449
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 3 cut(s) 252, 300, 528
BseDI CCNNGG 2 cut(s) 299, 414
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 2 cut(s) 122, 373
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 5 cut(s) 23, 87, 123, 187, 526
BseNI ACTGG 2 cut(s) 116, 449
BseXI GCAGC 2 cut(s) 518, 528
BsgI GTGCAG 1 cut(s) 167
BshFI GGCC 1 cut(s) 5
BslI CCNNNNNNNGG 2 cut(s) 122, 373
BsmAI GTCTC 1 cut(s) 410
BsmBI CGTCTC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 218
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 5 cut(s) 22, 88, 122, 186, 525
BspLI GGNNCC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 2 cut(s) 116, 449
BssECI CCNNGG 2 cut(s) 299, 414
BssT1I CCWWGG 1 cut(s) 414
Bst2UI CCWGG 3 cut(s) 252, 300, 528
Bst4CI ACNGT 2 cut(s) 352, 404
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 5 cut(s) 9, 96, 109, 173, 512
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 1 cut(s) 325
BstHHI GCGC 1 cut(s) 463
BstMAI GTCTC 1 cut(s) 410
BstMWI GCNNNNNNNGC 4 cut(s) 32, 239, 469, 506
BstNI CCWGG 3 cut(s) 252, 300, 528
BstNSI RCATGY 1 cut(s) 131
BstPI GGTNACC 1 cut(s) 223
BstSCI CCNGG 3 cut(s) 250, 298, 526
BstV1I GCAGC 2 cut(s) 518, 528
BstV2I GAAGAC 1 cut(s) 616
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 325
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 4 cut(s) 123, 181, 357, 558
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 113
CviAII CATG 1 cut(s) 128
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 5 cut(s) 9, 96, 109, 173, 512
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 62, 407
Eco91I GGTNACC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 139
EcoO65I GGTNACC 1 cut(s) 223
EcoRII CCWGG 3 cut(s) 250, 298, 526
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 410
FaeI CATG 1 cut(s) 131
FaiI YATR 5 cut(s) 38, 129, 294, 308, 620
FatI CATG 1 cut(s) 127
FblI GTMKAC 1 cut(s) 101
Fnu4HI GCNGC 2 cut(s) 507, 517
FokI GGATG 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 507, 517
FspBI CTAG 5 cut(s) 188, 278, 330, 368, 458
GlaI GCGC 1 cut(s) 462
GluI GCNGC 2 cut(s) 507, 517
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 463
Hin1II CATG 1 cut(s) 131
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 3 cut(s) 98, 247, 255
HphI GGTGA 3 cut(s) 235, 376, 443
Hpy166II GTNNAC 2 cut(s) 102, 179
Hpy188I TCNGA 2 cut(s) 64, 426
Hpy188III TCNNGA 2 cut(s) 80, 346
Hpy8I GTNNAC 2 cut(s) 102, 179
HpyAV CCTTC 3 cut(s) 214, 353, 421
HpyCH4III ACNGT 2 cut(s) 352, 404
HpyCH4V TGCA 6 cut(s) 135, 184, 296, 494, 506, 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 239, 469, 506
HpyF3I CTNAG 5 cut(s) 9, 96, 109, 173, 512
Hsp92II CATG 1 cut(s) 131
HspAI GCGC 1 cut(s) 461
LmnI GCTCC 1 cut(s) 319
Lsp1109I GCAGC 2 cut(s) 518, 528
MaeI CTAG 5 cut(s) 188, 278, 330, 368, 458
MaeIII GTNAC 2 cut(s) 223, 382
MboII GAAGA 3 cut(s) 68, 160, 616
MlyI GAGTC 2 cut(s) 107, 249
MmeI TCCRAC 2 cut(s) 414, 460
MnlI CCTC 3 cut(s) 16, 182, 397
MspR9I CCNGG 3 cut(s) 252, 300, 528
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 3 cut(s) 252, 300, 528
MwoI GCNNNNNNNGC 4 cut(s) 32, 239, 469, 506
NlaIII CATG 1 cut(s) 131
NlaIV GGNNCC 1 cut(s) 141
NmuCI GTSAC 2 cut(s) 223, 382
NspI RCATGY 1 cut(s) 131
NspV TTCGAA 1 cut(s) 218
PciI ACATGT 1 cut(s) 127
PctI GAATGC 1 cut(s) 26
PfeI GAWTC 1 cut(s) 247
PflMI CCANNNNNTGG 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 2 cut(s) 508, 518
PleI GAGTC 2 cut(s) 106, 249
PpsI GAGTC 2 cut(s) 106, 249
PpuMI RGGWCCY 1 cut(s) 139
PscI ACATGT 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 250, 298, 526
PspEI GGTNACC 1 cut(s) 223
PspGI CCWGG 3 cut(s) 250, 298, 526
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 139
PspPPI RGGWCCY 1 cut(s) 139
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SatI GCNGC 2 cut(s) 507, 517
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 2 cut(s) 107, 249
ScrFI CCNGG 3 cut(s) 252, 300, 528
SfuI TTCGAA 1 cut(s) 218
SinI GGWCC 1 cut(s) 139
SspMI CTAG 5 cut(s) 188, 278, 330, 368, 458
StyD4I CCNGG 3 cut(s) 250, 298, 526
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 352, 404
TaqI TCGA 4 cut(s) 153, 218, 288, 627
TfiI GAWTC 1 cut(s) 247
TscAI CASTG 4 cut(s) 123, 181, 357, 558
TseFI GTSAC 2 cut(s) 223, 382
TseI GCWGC 2 cut(s) 506, 516
Tsp45I GTSAC 2 cut(s) 223, 382
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 4 cut(s) 123, 181, 357, 558
Van91I CCANNNNNTGG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 139
XceI RCATGY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 101
XspI CTAG 5 cut(s) 188, 278, 330, 368, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.