Rmu_sc0005319.1_g000003

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005319.1
OG0000812
Physical Location & Seq
Forward (+)
8040 .. 8794
755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005319.1_g000003.1.cds

Sequence Viewer

Length: 651 bp
atggcctttaagtttctgttgttggcattccttgccataagtgcttgttccattgctttggcttctgacccaagttctcttcaggatttctgtgttgcggacccaacaggctcagtaccagtgaatggacatgtctgcaaggaccccaaggttgtcgaagccaatgacttcttcttcagtggacttcacctagcaggcaacacgtcaaaccctgctggttcaaaggtgacgcctgctaacgttgcccagattccaggacttaatacttttggtatctccctagttcgtatcgactatgcacgatggggtatcaatgctccacacactcatcctagagctactgaaatcttgacagtccttgaagggagcctcgaagtgggttttgtcacctccaaccccgaaaacaggcttatcacaaaggtgcttcagaagggcgatgtgtttgtgttcccagtaggacttgtgcatttccaacacaatgttggaaatgttaatgcggtcgccattgcagctctgagcagccaaaatccaggtgctattaccattgccaatgcagtgtttggatcaaagcctgccatttctgctgatgttcttgcaaaggcattccaagcggacaaggacacaatctatgatctccagtccaagttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

216

Amino Acids

22.75

Weight (kDa)

6.27

Isoelectric Point (pI)

26.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 125
AciI CCGC 3 cut(s) 98, 497, 611
AclI AACGTT 1 cut(s) 240
AclWI GGATC 1 cut(s) 571
AcuI CTGAAG 3 cut(s) 65, 160, 410
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 3 cut(s) 125, 376, 405
AflIII ACRYGT 2 cut(s) 130, 201
AgsI TTSAA 2 cut(s) 222, 362
AjiI CACGTC 1 cut(s) 204
AjnI CCWGG 2 cut(s) 253, 529
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 2 cut(s) 338, 512
AluI AGCT 2 cut(s) 338, 512
AlwI GGATC 1 cut(s) 571
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 509, 519
AspS9I GGNCC 2 cut(s) 100, 142
AsuHPI GGTGA 3 cut(s) 179, 238, 379
AvaII GGWCC 2 cut(s) 100, 142
BbvI GCAGC 2 cut(s) 521, 531
BccI CCATC 1 cut(s) 297
BciT130I CCWGG 2 cut(s) 255, 531
BfaI CTAG 4 cut(s) 191, 281, 333, 649
BisI GCNGC 2 cut(s) 510, 520
BlsI GCNGC 2 cut(s) 511, 521
Bme1390I CCNGG 2 cut(s) 255, 531
Bme18I GGWCC 2 cut(s) 100, 142
BmgBI CACGTC 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 100, 142
BmiI GGNNCC 3 cut(s) 102, 144, 368
BmrFI CCNGG 2 cut(s) 255, 531
BmrI ACTGGG 1 cut(s) 446
BmuI ACTGGG 1 cut(s) 446
BpmI CTGGAG 1 cut(s) 620
BsaHI GRCGYC 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 147
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 376, 405
Bse1I ACTGG 3 cut(s) 119, 452, 637
Bse3DI GCAATG 3 cut(s) 51, 504, 543
BseBI CCWGG 2 cut(s) 255, 531
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 328
BseLI CCNNNNNNNGG 3 cut(s) 125, 376, 405
BseMI GCAATG 3 cut(s) 51, 504, 543
BseMII CTCAG 2 cut(s) 126, 506
BseNI ACTGG 3 cut(s) 119, 452, 637
BseXI GCAGC 2 cut(s) 521, 531
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 501
BslI CCNNNNNNNGG 3 cut(s) 125, 376, 405
BsmI GAATGC 2 cut(s) 26, 602
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 563, 631
BspACI CCGC 3 cut(s) 98, 497, 611
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 125, 507
BspLI GGNNCC 3 cut(s) 102, 144, 368
BspPI GGATC 1 cut(s) 571
BsrDI GCAATG 3 cut(s) 51, 504, 543
BsrI ACTGG 3 cut(s) 119, 452, 637
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 2 cut(s) 563, 631
BssNI GRCGYC 1 cut(s) 230
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 2 cut(s) 255, 531
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 84
BstACI GRCGYC 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 3 cut(s) 196, 234, 573
BstDEI CTNAG 2 cut(s) 112, 515
BstF5I GGATG 1 cut(s) 328
BstKTI GATC 2 cut(s) 566, 634
BstMBI GATC 2 cut(s) 563, 631
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 6 cut(s) 32, 41, 242, 509, 581, 608
BstNI CCWGG 2 cut(s) 255, 531
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 253, 529
BstV1I GCAGC 2 cut(s) 521, 531
BstXI CCANNNNNNTGG 1 cut(s) 58
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 450
BtrI CACGTC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 3 cut(s) 126, 184, 561
Cac8I GCNNGC 3 cut(s) 196, 234, 573
Cfr13I GGNCC 2 cut(s) 100, 142
CseI GACGC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 116
CviAII CATG 1 cut(s) 131
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 2 cut(s) 112, 515
DpnI GATC 2 cut(s) 565, 633
DpnII GATC 2 cut(s) 563, 631
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 147
Eco47I GGWCC 2 cut(s) 100, 142
Eco57I CTGAAG 3 cut(s) 65, 160, 410
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 2 cut(s) 253, 529
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 1 cut(s) 134
FaiI YATR 4 cut(s) 38, 132, 297, 630
FatI CATG 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 510, 520
FokI GGATG 1 cut(s) 315
Fsp4HI GCNGC 2 cut(s) 510, 520
FspBI CTAG 4 cut(s) 191, 281, 333, 649
GluI GCNGC 2 cut(s) 510, 520
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 1 cut(s) 134
HinfI GANTC 1 cut(s) 250
HphI GGTGA 3 cut(s) 179, 238, 379
Hpy166II GTNNAC 1 cut(s) 182
Hpy188I TCNGA 3 cut(s) 67, 429, 516
Hpy188III TCNNGA 2 cut(s) 83, 349
Hpy8I GTNNAC 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 356, 424
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 2 cut(s) 203, 240
HpyCH4V TGCA 6 cut(s) 138, 299, 466, 509, 554, 596
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 41, 242, 509, 581, 608
HpyF3I CTNAG 2 cut(s) 112, 515
HpySE526I ACGT 2 cut(s) 203, 240
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 1 cut(s) 134
Kzo9I GATC 2 cut(s) 563, 631
LmnI GCTCC 2 cut(s) 322, 366
Lsp1109I GCAGC 2 cut(s) 521, 531
MaeI CTAG 4 cut(s) 191, 281, 333, 649
MaeII ACGT 2 cut(s) 203, 240
MaeIII GTNAC 2 cut(s) 226, 385
MalI GATC 2 cut(s) 565, 633
MboI GATC 2 cut(s) 563, 631
MboII GAAGA 3 cut(s) 71, 163, 166
MmeI TCCRAC 3 cut(s) 417, 463, 496
MnlI CCTC 2 cut(s) 380, 400
MseI TTAA 3 cut(s) 9, 261, 492
MslI CAYNNNNRTG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 255, 531
Mva1269I GAATGC 2 cut(s) 26, 602
MvaI CCWGG 2 cut(s) 255, 531
MwoI GCNNNNNNNGC 6 cut(s) 32, 41, 242, 509, 581, 608
NdeII GATC 2 cut(s) 563, 631
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 3 cut(s) 102, 144, 368
NmuCI GTSAC 2 cut(s) 226, 385
NspI RCATGY 1 cut(s) 134
OliI CACNNNNGTG 1 cut(s) 419
PciI ACATGT 1 cut(s) 130
PctI GAATGC 2 cut(s) 26, 602
PfeI GAWTC 1 cut(s) 250
PflMI CCANNNNNTGG 1 cut(s) 125
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 2 cut(s) 511, 521
PpuMI RGGWCCY 1 cut(s) 142
PscI ACATGT 1 cut(s) 130
Psp1406I AACGTT 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 2 cut(s) 253, 529
PspGI CCWGG 2 cut(s) 253, 529
PspN4I GGNNCC 3 cut(s) 102, 144, 368
PspPI GGNCC 2 cut(s) 100, 142
PspPPI RGGWCCY 1 cut(s) 142
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 3 cut(s) 9, 261, 492
SatI GCNGC 2 cut(s) 510, 520
Sau3AI GATC 2 cut(s) 563, 631
Sau96I GGNCC 2 cut(s) 100, 142
ScrFI CCNGG 2 cut(s) 255, 531
SinI GGWCC 2 cut(s) 100, 142
SmiMI CAYNNNNRTG 1 cut(s) 419
SsiI CCGC 3 cut(s) 98, 497, 611
SspMI CTAG 4 cut(s) 191, 281, 333, 649
StyD4I CCNGG 2 cut(s) 253, 529
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 2 cut(s) 206, 243
TaqI TCGA 3 cut(s) 156, 291, 372
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 3 cut(s) 9, 261, 492
Tru9I TTAA 3 cut(s) 9, 261, 492
TscAI CASTG 3 cut(s) 126, 184, 561
TseFI GTSAC 2 cut(s) 226, 385
TseI GCWGC 2 cut(s) 509, 519
Tsp45I GTSAC 2 cut(s) 226, 385
TspRI CASTG 3 cut(s) 126, 184, 561
Van91I CCANNNNNTGG 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 100, 142
XceI RCATGY 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XspI CTAG 4 cut(s) 191, 281, 333, 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.