Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 155 |
| AccII | CGCG | 1 cut(s) | 185 |
| AciI | CCGC | 2 cut(s) | 153, 185 |
| AcsI | RAATTY | 1 cut(s) | 177 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 342 |
| AgsI | TTSAA | 3 cut(s) | 166, 544, 631 |
| AluBI | AGCT | 2 cut(s) | 399, 559 |
| AluI | AGCT | 2 cut(s) | 399, 559 |
| Alw21I | GWGCWC | 1 cut(s) | 401 |
| Alw26I | GTCTC | 1 cut(s) | 474 |
| Ama87I | CYCGRG | 1 cut(s) | 394 |
| AoxI | GGCC | 2 cut(s) | 349, 386 |
| ApaI | GGGCCC | 1 cut(s) | 390 |
| ApeKI | GCWGC | 1 cut(s) | 507 |
| ApoI | RAATTY | 1 cut(s) | 177 |
| AspLEI | GCGC | 1 cut(s) | 185 |
| AspS9I | GGNCC | 2 cut(s) | 386, 387 |
| AvaI | CYCGRG | 1 cut(s) | 394 |
| BaeGI | GKGCMC | 1 cut(s) | 390 |
| BanII | GRGCYC | 3 cut(s) | 390, 401, 421 |
| BbsI | GAAGAC | 1 cut(s) | 456 |
| Bbv12I | GWGCWC | 1 cut(s) | 401 |
| BbvI | GCAGC | 1 cut(s) | 519 |
| BccI | CCATC | 3 cut(s) | 52, 278, 517 |
| BceAI | ACGGC | 2 cut(s) | 287, 520 |
| BcoDI | GTCTC | 1 cut(s) | 474 |
| BfaI | CTAG | 3 cut(s) | 146, 597, 611 |
| BfmI | CTRYAG | 1 cut(s) | 654 |
| BisI | GCNGC | 1 cut(s) | 508 |
| BlsI | GCNGC | 1 cut(s) | 509 |
| BmeT110I | CYCGRG | 1 cut(s) | 394 |
| BmgT120I | GGNCC | 2 cut(s) | 386, 387 |
| BmiI | GGNNCC | 1 cut(s) | 388 |
| BmsI | GCATC | 3 cut(s) | 54, 158, 640 |
| BpiI | GAAGAC | 1 cut(s) | 456 |
| BpuEI | CTTGAG | 1 cut(s) | 405 |
| BsaJI | CCNNGG | 1 cut(s) | 382 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 342 |
| BseDI | CCNNGG | 1 cut(s) | 382 |
| BseGI | GGATG | 1 cut(s) | 149 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 342 |
| BseMII | CTCAG | 3 cut(s) | 170, 485, 635 |
| BseRI | GAGGAG | 1 cut(s) | 322 |
| BseSI | GKGCMC | 1 cut(s) | 390 |
| BseXI | GCAGC | 1 cut(s) | 519 |
| Bsh1236I | CGCG | 1 cut(s) | 185 |
| BshFI | GGCC | 2 cut(s) | 351, 388 |
| BsiHKAI | GWGCWC | 1 cut(s) | 401 |
| BsiHKCI | CYCGRG | 1 cut(s) | 394 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 342 |
| BsmAI | GTCTC | 1 cut(s) | 474 |
| BsnI | GGCC | 2 cut(s) | 351, 388 |
| BsoBI | CYCGRG | 1 cut(s) | 394 |
| Bsp120I | GGGCCC | 1 cut(s) | 386 |
| Bsp1286I | GDGCHC | 3 cut(s) | 390, 401, 421 |
| Bsp143I | GATC | 2 cut(s) | 72, 537 |
| BspACI | CCGC | 2 cut(s) | 153, 185 |
| BspANI | GGCC | 2 cut(s) | 351, 388 |
| BspCNI | CTCAG | 3 cut(s) | 169, 484, 634 |
| BspFNI | CGCG | 1 cut(s) | 185 |
| BspLI | GGNNCC | 1 cut(s) | 388 |
| BsrBI | CCGCTC | 1 cut(s) | 155 |
| BssECI | CCNNGG | 1 cut(s) | 382 |
| BssMI | GATC | 2 cut(s) | 72, 537 |
| BssT1I | CCWWGG | 1 cut(s) | 382 |
| Bst4CI | ACNGT | 1 cut(s) | 41 |
| BstDEI | CTNAG | 4 cut(s) | 6, 156, 471, 621 |
| BstF5I | GGATG | 1 cut(s) | 149 |
| BstFNI | CGCG | 1 cut(s) | 185 |
| BstHHI | GCGC | 1 cut(s) | 185 |
| BstKTI | GATC | 2 cut(s) | 75, 540 |
| BstMAI | GTCTC | 1 cut(s) | 474 |
| BstMBI | GATC | 2 cut(s) | 72, 537 |
| BstSFI | CTRYAG | 1 cut(s) | 654 |
| BstSLI | GKGCMC | 1 cut(s) | 390 |
| BstUI | CGCG | 1 cut(s) | 185 |
| BstV1I | GCAGC | 1 cut(s) | 519 |
| BstV2I | GAAGAC | 1 cut(s) | 456 |
| BsuRI | GGCC | 2 cut(s) | 351, 388 |
| BtsCI | GGATG | 1 cut(s) | 149 |
| BtsIMutI | CAGTG | 1 cut(s) | 216 |
| CfoI | GCGC | 1 cut(s) | 185 |
| Cfr13I | GGNCC | 2 cut(s) | 386, 387 |
| CviAII | CATG | 1 cut(s) | 491 |
| CviJI | RGCY | 6 cut(s) | 5, 351, 388, 399, 419, 559 |
| CviKI_1 | RGCY | 6 cut(s) | 5, 351, 388, 399, 419, 559 |
| DdeI | CTNAG | 4 cut(s) | 6, 156, 471, 621 |
| DpnI | GATC | 2 cut(s) | 74, 539 |
| DpnII | GATC | 2 cut(s) | 72, 537 |
| DraI | TTTAAA | 1 cut(s) | 234 |
| Ecl136II | GAGCTC | 1 cut(s) | 399 |
| Eco130I | CCWWGG | 1 cut(s) | 382 |
| Eco24I | GRGCYC | 3 cut(s) | 390, 401, 421 |
| Eco53kI | GAGCTC | 1 cut(s) | 399 |
| Eco88I | CYCGRG | 1 cut(s) | 394 |
| EcoICRI | GAGCTC | 1 cut(s) | 399 |
| EcoO109I | RGGNCCY | 1 cut(s) | 386 |
| EcoT14I | CCWWGG | 1 cut(s) | 382 |
| EcoT38I | GRGCYC | 3 cut(s) | 390, 401, 421 |
| ErhI | CCWWGG | 1 cut(s) | 382 |
| FaeI | CATG | 1 cut(s) | 494 |
| FatI | CATG | 1 cut(s) | 490 |
| Fnu4HI | GCNGC | 1 cut(s) | 508 |
| FokI | GGATG | 1 cut(s) | 136 |
| FriOI | GRGCYC | 3 cut(s) | 390, 401, 421 |
| Fsp4HI | GCNGC | 1 cut(s) | 508 |
| FspBI | CTAG | 3 cut(s) | 146, 597, 611 |
| GlaI | GCGC | 1 cut(s) | 184 |
| GluI | GCNGC | 1 cut(s) | 508 |
| HaeIII | GGCC | 2 cut(s) | 351, 388 |
| HhaI | GCGC | 1 cut(s) | 185 |
| Hin1II | CATG | 1 cut(s) | 494 |
| Hin6I | GCGC | 1 cut(s) | 183 |
| HinP1I | GCGC | 1 cut(s) | 183 |
| Hpy166II | GTNNAC | 2 cut(s) | 214, 581 |
| Hpy188I | TCNGA | 3 cut(s) | 57, 474, 522 |
| Hpy8I | GTNNAC | 2 cut(s) | 214, 581 |
| Hpy99I | CGWCG | 1 cut(s) | 278 |
| HpyAV | CCTTC | 1 cut(s) | 408 |
| HpyCH4III | ACNGT | 1 cut(s) | 41 |
| HpyCH4IV | ACGT | 1 cut(s) | 276 |
| HpyCH4V | TGCA | 2 cut(s) | 316, 682 |
| HpyF3I | CTNAG | 4 cut(s) | 6, 156, 471, 621 |
| HpySE526I | ACGT | 1 cut(s) | 276 |
| Hsp92II | CATG | 1 cut(s) | 494 |
| HspAI | GCGC | 1 cut(s) | 183 |
| Kzo9I | GATC | 2 cut(s) | 72, 537 |
| LmnI | GCTCC | 1 cut(s) | 556 |
| LpnPI | CCDG | 4 cut(s) | 11, 109, 395, 574 |
| Lsp1109I | GCAGC | 1 cut(s) | 519 |
| LweI | GCATC | 3 cut(s) | 54, 158, 640 |
| MaeI | CTAG | 3 cut(s) | 146, 597, 611 |
| MaeII | ACGT | 1 cut(s) | 276 |
| MaeIII | GTNAC | 2 cut(s) | 304, 613 |
| MalI | GATC | 2 cut(s) | 74, 539 |
| MbiI | CCGCTC | 1 cut(s) | 155 |
| MboI | GATC | 2 cut(s) | 72, 537 |
| MboII | GAAGA | 4 cut(s) | 200, 461, 532, 659 |
| MhlI | GDGCHC | 3 cut(s) | 390, 401, 421 |
| MluCI | AATT | 1 cut(s) | 177 |
| MseI | TTAA | 3 cut(s) | 78, 233, 531 |
| MvnI | CGCG | 1 cut(s) | 185 |
| NdeII | GATC | 2 cut(s) | 72, 537 |
| NlaIII | CATG | 1 cut(s) | 494 |
| NlaIV | GGNNCC | 1 cut(s) | 388 |
| PkrI | GCNGC | 1 cut(s) | 509 |
| Psp124BI | GAGCTC | 1 cut(s) | 401 |
| PspN4I | GGNNCC | 1 cut(s) | 388 |
| PspOMI | GGGCCC | 1 cut(s) | 386 |
| PspPI | GGNCC | 2 cut(s) | 386, 387 |
| SacI | GAGCTC | 1 cut(s) | 401 |
| SaqAI | TTAA | 3 cut(s) | 78, 233, 531 |
| SatI | GCNGC | 1 cut(s) | 508 |
| Sau3AI | GATC | 2 cut(s) | 72, 537 |
| Sau96I | GGNCC | 2 cut(s) | 386, 387 |
| SduI | GDGCHC | 3 cut(s) | 390, 401, 421 |
| SetI | ASST | 7 cut(s) | 96, 128, 279, 347, 401, 561, 593 |
| SfaNI | GCATC | 3 cut(s) | 54, 158, 640 |
| SfcI | CTRYAG | 1 cut(s) | 654 |
| SmlI | CTYRAG | 1 cut(s) | 420 |
| SmoI | CTYRAG | 1 cut(s) | 420 |
| Sse9I | AATT | 1 cut(s) | 177 |
| SsiI | CCGC | 2 cut(s) | 153, 185 |
| SspMI | CTAG | 3 cut(s) | 146, 597, 611 |
| SstI | GAGCTC | 1 cut(s) | 401 |
| StyI | CCWWGG | 1 cut(s) | 382 |
| TaaI | ACNGT | 1 cut(s) | 41 |
| TaiI | ACGT | 1 cut(s) | 279 |
| TaqI | TCGA | 3 cut(s) | 71, 366, 536 |
| TasI | AATT | 1 cut(s) | 177 |
| Tru1I | TTAA | 3 cut(s) | 78, 233, 531 |
| Tru9I | TTAA | 3 cut(s) | 78, 233, 531 |
| TscAI | CASTG | 1 cut(s) | 216 |
| TseI | GCWGC | 1 cut(s) | 507 |
| TspDTI | ATGAA | 1 cut(s) | 479 |
| TspRI | CASTG | 1 cut(s) | 216 |
| XapI | RAATTY | 1 cut(s) | 177 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 520 |
| XspI | CTAG | 3 cut(s) | 146, 597, 611 |