Rh5AG524100

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000726
Physical Location & Seq
Forward (+)
89870693 .. 89882227
11535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG524100.1

Sequence Viewer

Length: 597 bp
ATGGCCAGAATACCTCCCATCTTTGCTACACAGTTCATAGTTCTCTCCCTTCTTTCTTCCTTTGCCCTAATTCTGGTCCAGGGAGAAGATGATCACGATTTCGTGAGAGCCATGGACCGCGATCTCTTGGGGCTCAAGAAAGAAAAGCTCAGCCACTTCAAGTTGTACTGGCATGACATTTTGAGCGGCAAAAACCCAAGCTCAATCGGAGTGGTGAAATCACCCGTTAACTCATCAACGCTCTTCGGATTCGTGAACATGATCGACAATCCCCTCACCGAAAAGCCGGAGCTGAGCTCGAAGCTGCTGGGGAGGGCTCAAGGGTTCTACGCATCAGCTTCACAACAGGAGTTCGGGCTGTTGATGGCCATGAACTTTCATTTTATTCAGGGCAAGTACAACGGGAGCACCATAACTGTGCTGGGGAGGAACCCGGTGTTCAACAAGGTGAGGGAAATGCCGGTGATCGGAGGAAGTGGACTTTTCAGGTTTGCAAGAGGTTATGTTGAAGCAAGAACTCACACATTCAATCTGAGCACCCGAAATGCCATCGTTGAAAATCCAGGAGATACTGAAGCTTCTGATTACTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

198

Amino Acids

22.07

Weight (kDa)

9.15

Isoelectric Point (pI)

27.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 186 2.4e-53 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 1 cut(s) 120
AciI CCGC 2 cut(s) 118, 186
AcoI YGGCCR 2 cut(s) 3, 366
AcuI CTGAAG 1 cut(s) 594
AfaI GTAC 2 cut(s) 167, 398
AfiI CCNNNNNNNGG 2 cut(s) 73, 467
AgsI TTSAA 6 cut(s) 160, 442, 509, 529, 557, 592
AjnI CCWGG 2 cut(s) 78, 562
AluBI AGCT 7 cut(s) 148, 201, 292, 297, 304, 338, 578
AluI AGCT 7 cut(s) 148, 201, 292, 297, 304, 338, 578
Alw21I GWGCWC 3 cut(s) 299, 410, 539
AoxI GGCC 2 cut(s) 3, 366
ApeKI GCWGC 1 cut(s) 304
AspS9I GGNCC 2 cut(s) 76, 115
AsuC2I CCSGG 1 cut(s) 434
AsuHPI GGTGA 5 cut(s) 213, 226, 268, 460, 475
AvaII GGWCC 2 cut(s) 76, 115
BalI TGGCCA 2 cut(s) 5, 368
BanII GRGCYC 3 cut(s) 135, 299, 319
Bbv12I GWGCWC 3 cut(s) 299, 410, 539
BbvI GCAGC 1 cut(s) 291
BccI CCATC 3 cut(s) 26, 358, 557
BciT130I CCWGG 2 cut(s) 80, 564
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 1 cut(s) 434
BisI GCNGC 2 cut(s) 187, 305
BlpI GCTNAGC 2 cut(s) 149, 293
BlsI GCNGC 2 cut(s) 188, 306
Bme1390I CCNGG 3 cut(s) 80, 434, 564
Bme18I GGWCC 2 cut(s) 76, 115
BmgT120I GGNCC 2 cut(s) 76, 115
BmiI GGNNCC 1 cut(s) 431
BmrFI CCNGG 3 cut(s) 80, 434, 564
BmsI GCATC 1 cut(s) 341
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 2 cut(s) 149, 293
BpuEI CTTGAG 2 cut(s) 119, 303
BpuMI CCSGG 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 79, 111
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 467
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 2 cut(s) 80, 564
BseDI CCNNGG 2 cut(s) 79, 111
BseLI CCNNNNNNNGG 2 cut(s) 73, 467
BseMII CTCAG 3 cut(s) 163, 284, 524
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 291
BseYI CCCAGC 2 cut(s) 307, 421
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 5, 368
BsiHKAI GWGCWC 3 cut(s) 299, 410, 539
BsiSI CCGG 3 cut(s) 287, 434, 461
BslI CCNNNNNNNGG 2 cut(s) 73, 467
BsnI GGCC 2 cut(s) 5, 368
Bsp1286I GDGCHC 5 cut(s) 135, 299, 319, 410, 539
Bsp143I GATC 4 cut(s) 91, 121, 261, 465
Bsp1720I GCTNAGC 2 cut(s) 149, 293
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 2 cut(s) 118, 186
BspANI GGCC 2 cut(s) 5, 368
BspCNI CTCAG 3 cut(s) 162, 285, 525
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 431
BspQI GCTCTTC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 79, 111
BssMI GATC 4 cut(s) 91, 121, 261, 465
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 2 cut(s) 80, 564
Bst4CI ACNGT 2 cut(s) 33, 418
Bst6I CTCTTC 1 cut(s) 248
BstDEI CTNAG 3 cut(s) 149, 293, 533
BstDSI CCRYGG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 4 cut(s) 94, 124, 264, 468
BstMBI GATC 4 cut(s) 91, 121, 261, 465
BstNI CCWGG 2 cut(s) 80, 564
BstSCI CCNGG 3 cut(s) 78, 432, 562
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 291
BsuRI GGCC 2 cut(s) 5, 368
BtgI CCRYGG 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 76, 115
Csp6I GTAC 2 cut(s) 166, 397
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 4 cut(s) 112, 173, 259, 370
CviQI GTAC 2 cut(s) 166, 397
DdeI CTNAG 3 cut(s) 149, 293, 533
DpnI GATC 4 cut(s) 93, 123, 263, 467
DpnII GATC 4 cut(s) 91, 121, 261, 465
EaeI YGGCCR 2 cut(s) 3, 366
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 3 cut(s) 135, 299, 319
Eco47I GGWCC 2 cut(s) 76, 115
Eco53kI GAGCTC 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 594
EcoICRI GAGCTC 1 cut(s) 297
EcoRII CCWGG 2 cut(s) 78, 562
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 3 cut(s) 135, 299, 319
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 4 cut(s) 115, 176, 262, 373
FaiI YATR 7 cut(s) 38, 113, 174, 260, 371, 413, 504
FatI CATG 4 cut(s) 111, 172, 258, 369
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 187, 305
FriOI GRGCYC 3 cut(s) 135, 299, 319
Fsp4HI GCNGC 2 cut(s) 187, 305
GluI GCNGC 2 cut(s) 187, 305
GsaI CCCAGC 2 cut(s) 311, 425
HaeIII GGCC 2 cut(s) 5, 368
HapII CCGG 3 cut(s) 287, 434, 461
Hin1II CATG 4 cut(s) 115, 176, 262, 373
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 576
HinfI GANTC 1 cut(s) 249
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 3 cut(s) 287, 434, 461
HphI GGTGA 5 cut(s) 213, 226, 268, 460, 475
Hpy166II GTNNAC 3 cut(s) 229, 256, 479
Hpy188I TCNGA 5 cut(s) 209, 248, 470, 534, 583
Hpy188III TCNNGA 4 cut(s) 95, 103, 136, 253
Hpy8I GTNNAC 3 cut(s) 229, 256, 479
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 33, 418
HpyCH4V TGCA 1 cut(s) 494
HpyF3I CTNAG 3 cut(s) 149, 293, 533
Hsp92II CATG 4 cut(s) 115, 176, 262, 373
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 4 cut(s) 91, 121, 261, 465
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 2 cut(s) 289, 405
Lsp1109I GCAGC 1 cut(s) 291
LweI GCATC 1 cut(s) 341
MalI GATC 4 cut(s) 93, 123, 263, 467
MbiI CCGCTC 1 cut(s) 186
MboI GATC 4 cut(s) 91, 121, 261, 465
MboII GAAGA 3 cut(s) 48, 98, 235
MhlI GDGCHC 5 cut(s) 135, 299, 319, 410, 539
MlsI TGGCCA 2 cut(s) 5, 368
MluCI AATT 1 cut(s) 69
MluNI TGGCCA 2 cut(s) 5, 368
MnlI CCTC 7 cut(s) 24, 284, 306, 420, 444, 464, 491
Mox20I TGGCCA 2 cut(s) 5, 368
MscI TGGCCA 2 cut(s) 5, 368
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 2 cut(s) 5, 368
MspI CCGG 3 cut(s) 287, 434, 461
MspR9I CCNGG 3 cut(s) 80, 434, 564
MvaI CCWGG 2 cut(s) 80, 564
MvnI CGCG 1 cut(s) 120
NciI CCSGG 1 cut(s) 434
NcoI CCATGG 1 cut(s) 111
NdeII GATC 4 cut(s) 91, 121, 261, 465
NlaIII CATG 4 cut(s) 115, 176, 262, 373
NlaIV GGNNCC 1 cut(s) 431
PciSI GCTCTTC 1 cut(s) 248
PfeI GAWTC 1 cut(s) 249
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 2 cut(s) 188, 306
Psp124BI GAGCTC 1 cut(s) 299
Psp6I CCWGG 2 cut(s) 78, 562
PspFI CCCAGC 2 cut(s) 307, 421
PspGI CCWGG 2 cut(s) 78, 562
PspN4I GGNNCC 1 cut(s) 431
PspPI GGNCC 2 cut(s) 76, 115
RsaI GTAC 2 cut(s) 167, 398
RsaNI GTAC 2 cut(s) 166, 397
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 187, 305
Sau3AI GATC 4 cut(s) 91, 121, 261, 465
Sau96I GGNCC 2 cut(s) 76, 115
ScrFI CCNGG 3 cut(s) 80, 434, 564
SduI GDGCHC 5 cut(s) 135, 299, 319, 410, 539
SfaNI GCATC 1 cut(s) 341
SinI GGWCC 2 cut(s) 76, 115
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 134, 318
SmoI CTYRAG 2 cut(s) 134, 318
Sse9I AATT 1 cut(s) 69
SsiI CCGC 2 cut(s) 118, 186
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 3 cut(s) 78, 432, 562
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 33, 418
TaqI TCGA 2 cut(s) 264, 299
TasI AATT 1 cut(s) 69
TatI WGTACW 2 cut(s) 165, 396
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseI GCWGC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 25, 368, 386
VpaK11BI GGWCC 2 cut(s) 76, 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.