pycom03g00940

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000726
Physical Location & Seq
Forward (+)
749156 .. 749542
387 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00940.1

Sequence Viewer

Length: 387 bp
ATGATTGATGATCCACTTACTGAAGGGCCTGAAATGAGCTCAAAATTGTTGGGAAGGGCTCAAGGGTTTTATGGATCTGCTTCACAAAAAGAGGCTGGCCTATTGCTGGCCATGAATTTTGCTTTCATTCAAGGCAAGTATAATGGTAGCACCATAACTGTGCTAGGAAGGAACCAAGTATTCAACAAGGTGAGGGAGATGCTAGTGATCGGAGGCAGCGGGCTTTTCTGGTTTGCGACGGGTTATGTTGAAGCAAGAACTCACTATTTCAACCTCACTACTGGGGATGCAGTTGAGTATAATGTCTATGTGCTGCATTACTTATCATTCTTATTTTCTGGGCACATTTACTTTTTCCTTTGCTTGTCTAGCTTGCTTTTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

129

Amino Acids

14.31

Weight (kDa)

6.25

Isoelectric Point (pI)

30.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 1 - 104 1.9e-39 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 219
AclWI GGATC 2 cut(s) 5, 82
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 115
AcuI CTGAAG 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 4 cut(s) 131, 184, 251, 271
AluBI AGCT 2 cut(s) 39, 372
AluI AGCT 2 cut(s) 39, 372
Alw21I GWGCWC 1 cut(s) 41
AlwI GGATC 2 cut(s) 5, 82
AoxI GGCC 3 cut(s) 26, 97, 108
ApeKI GCWGC 2 cut(s) 216, 313
ApoI RAATTY 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 1 cut(s) 110
BanII GRGCYC 2 cut(s) 41, 61
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 228, 300
BfaI CTAG 3 cut(s) 164, 203, 369
BisI GCNGC 2 cut(s) 217, 314
BlsI GCNGC 2 cut(s) 218, 315
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 2 cut(s) 189, 277
BmuI ACTGGG 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 286
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 1 cut(s) 106
BseNI ACTGG 1 cut(s) 286
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 2 cut(s) 228, 300
BshFI GGCC 3 cut(s) 28, 99, 110
BsiHKAI GWGCWC 1 cut(s) 41
BslI CCNNNNNNNGG 1 cut(s) 106
BsnI GGCC 3 cut(s) 28, 99, 110
Bsp1286I GDGCHC 3 cut(s) 41, 61, 345
Bsp143I GATC 3 cut(s) 10, 74, 207
BspACI CCGC 1 cut(s) 219
BspANI GGCC 3 cut(s) 28, 99, 110
BspLI GGNNCC 1 cut(s) 173
BspPI GGATC 2 cut(s) 5, 82
BsrI ACTGG 1 cut(s) 286
BssMI GATC 3 cut(s) 10, 74, 207
Bst4CI ACNGT 1 cut(s) 160
BstC8I GCNNGC 4 cut(s) 97, 108, 221, 374
BstF5I GGATG 1 cut(s) 292
BstKTI GATC 3 cut(s) 13, 77, 210
BstMBI GATC 3 cut(s) 10, 74, 207
BstMWI GCNNNNNNNGC 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 228, 300
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
BsuRI GGCC 3 cut(s) 28, 99, 110
BtsCI GGATG 1 cut(s) 292
Cac8I GCNNGC 4 cut(s) 97, 108, 221, 374
Cfr13I GGNCC 1 cut(s) 26
CviAII CATG 1 cut(s) 112
CviJI RGCY 8 cut(s) 28, 39, 59, 95, 99, 110, 223, 372
CviKI_1 RGCY 8 cut(s) 28, 39, 59, 95, 99, 110, 223, 372
DpnI GATC 3 cut(s) 12, 76, 209
DpnII GATC 3 cut(s) 10, 74, 207
EaeI YGGCCR 1 cut(s) 108
Ecl136II GAGCTC 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 41, 61
Eco53kI GAGCTC 1 cut(s) 39
Eco57I CTGAAG 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 26
EcoT38I GRGCYC 2 cut(s) 41, 61
FaeI CATG 1 cut(s) 115
FaiI YATR 7 cut(s) 72, 113, 141, 155, 246, 300, 309
FatI CATG 1 cut(s) 111
FauI CCCGC 1 cut(s) 212
Fnu4HI GCNGC 2 cut(s) 217, 314
FokI GGATG 1 cut(s) 299
FriOI GRGCYC 2 cut(s) 41, 61
Fsp4HI GCNGC 2 cut(s) 217, 314
FspBI CTAG 3 cut(s) 164, 203, 369
GluI GCNGC 2 cut(s) 217, 314
HaeIII GGCC 3 cut(s) 28, 99, 110
Hin1II CATG 1 cut(s) 115
HphI GGTGA 1 cut(s) 202
Hpy188I TCNGA 1 cut(s) 212
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 3 cut(s) 17, 48, 162
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 2 cut(s) 290, 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 369
Hsp92II CATG 1 cut(s) 115
Kzo9I GATC 3 cut(s) 10, 74, 207
LpnPI CCDG 6 cut(s) 42, 81, 92, 214, 267, 324
Lsp1109I GCAGC 2 cut(s) 228, 300
LweI GCATC 2 cut(s) 189, 277
MaeI CTAG 3 cut(s) 164, 203, 369
MalI GATC 3 cut(s) 12, 76, 209
MboI GATC 3 cut(s) 10, 74, 207
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 3 cut(s) 41, 61, 345
MlsI TGGCCA 1 cut(s) 110
MluCI AATT 2 cut(s) 44, 115
MluNI TGGCCA 1 cut(s) 110
MnlI CCTC 4 cut(s) 85, 186, 206, 284
Mox20I TGGCCA 1 cut(s) 110
MscI TGGCCA 1 cut(s) 110
MseI TTAA 1 cut(s) 385
MslI CAYNNNNRTG 1 cut(s) 158
Msp20I TGGCCA 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 219
MwoI GCNNNNNNNGC 1 cut(s) 369
NdeII GATC 3 cut(s) 10, 74, 207
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 1 cut(s) 173
PkrI GCNGC 2 cut(s) 218, 315
Psp124BI GAGCTC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 173
PspPI GGNCC 1 cut(s) 26
PsuI RGATCY 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 158
SacI GAGCTC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 385
SatI GCNGC 2 cut(s) 217, 314
Sau3AI GATC 3 cut(s) 10, 74, 207
Sau96I GGNCC 1 cut(s) 26
SduI GDGCHC 3 cut(s) 41, 61, 345
SetI ASST 4 cut(s) 41, 192, 276, 374
SfaNI GCATC 2 cut(s) 189, 277
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 60
SmoI CTYRAG 1 cut(s) 60
Sse9I AATT 2 cut(s) 44, 115
SsiI CCGC 1 cut(s) 219
SspMI CTAG 3 cut(s) 164, 203, 369
SstI GAGCTC 1 cut(s) 41
TaaI ACNGT 1 cut(s) 160
TasI AATT 2 cut(s) 44, 115
Tru1I TTAA 1 cut(s) 385
Tru9I TTAA 1 cut(s) 385
TseI GCWGC 2 cut(s) 216, 313
TspDTI ATGAA 2 cut(s) 115, 128
XapI RAATTY 1 cut(s) 115
XspI CTAG 3 cut(s) 164, 203, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.