pycom11g00810

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000726
Physical Location & Seq
Forward (+)
599432 .. 600221
790 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g00810.1

Sequence Viewer

Length: 732 bp
ATGACTCCACGATTTATATCACAACTAATTCCTTCTTATTATTCCATTCCAGTCAATAAAGGACTTTTGCTTAACGCGGTATTATTATTCTTTTGGCAAGTCGCATCATTTGAAAAAGACTTTTGCTTAACACATACCAGCAATACCATGGCTAGGCTGTCTCAAATGTCAGCTTTCCAATTCACAATTTTCTCCCTCCTCTCTTCCTTTACCTTGATCTTAGTTTCTGGAGAAAATTTAGGGTTTCACAGCACCGTGGACCGTAAGCTTCTCGGGCTGCAGAAAGAAAAGCTCACCCACGTTCGCTTATACTGGCATGACATTGTTGGTGGGTCCCATCCCTCTGCCATAGAGGTTGTCCCGGCACCCTCCAATAAGTCCCAGACGAATTTTGGGTTAGTGAGAATGTTTGATAATGCACTAACTGAAGGTCCTGAGTTGAGCTCAAAATTGGTGGCTAGGGCTCAAGGGTTTTACGGATTTGCAGGGCAGGTGGAGCTCAGCCTCCTGATGGCACAGAACTTTGCTTTTGTTGCAGGTGAGTTTAATGGAAGCACCATAACTTTGCTAGGAAGAAATTCAATATTAAACAAGGTGAGGGAGCTGCCTGTGATTGGTGGAAGTGGGGCTTTCAGGTTTGCTAGGGGTTATGCTGAATTGAGAACTCACACTTTTAATCTCACCTCAAGTGATGCTATCGTGGAGTATAATATTTACGTGTTGCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

244

Amino Acids

27.02

Weight (kDa)

9.01

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 99 - 240 2.4e-53 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 481, 527
Acc36I ACCTGC 2 cut(s) 481, 527
AccB1I GGYRCC 1 cut(s) 364
AccII CGCG 1 cut(s) 77
AciI CCGC 1 cut(s) 77
AcsI RAATTY 3 cut(s) 235, 388, 577
AcuI CTGAAG 1 cut(s) 447
AfiI CCNNNNNNNGG 3 cut(s) 153, 511, 614
AflIII ACRYGT 1 cut(s) 717
AgsI TTSAA 2 cut(s) 113, 582
AluBI AGCT 6 cut(s) 173, 268, 292, 444, 499, 604
AluI AGCT 6 cut(s) 173, 268, 292, 444, 499, 604
Alw21I GWGCWC 2 cut(s) 446, 501
Alw26I GTCTC 1 cut(s) 165
Ama87I CYCGRG 1 cut(s) 272
ApeKI GCWGC 2 cut(s) 277, 604
ApoI RAATTY 3 cut(s) 235, 388, 577
Asp700I GAANNNNTTC 1 cut(s) 577
AspS9I GGNCC 3 cut(s) 259, 333, 431
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 4 cut(s) 286, 551, 607, 673
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 3 cut(s) 259, 333, 431
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 3 cut(s) 446, 466, 501
Bbv12I GWGCWC 2 cut(s) 446, 501
BbvI GCAGC 2 cut(s) 264, 591
BccI CCATC 2 cut(s) 345, 505
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 4 cut(s) 153, 459, 569, 642
BfmI CTRYAG 1 cut(s) 278
BfuAI ACCTGC 2 cut(s) 481, 527
BisI GCNGC 2 cut(s) 278, 605
BlpI GCTNAGC 1 cut(s) 500
BlsI GCNGC 2 cut(s) 279, 606
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 3 cut(s) 259, 333, 431
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 3 cut(s) 259, 333, 431
BmiI GGNNCC 3 cut(s) 334, 335, 366
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 2 cut(s) 113, 682
BplI GAGNNNNNCTC 2 cut(s) 428, 460
BpmI CTGGAG 1 cut(s) 249
Bpu1102I GCTNAGC 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 450, 670
BpuMI CCSGG 1 cut(s) 362
BsaAI YACGTR 1 cut(s) 718
BsaBI GATNNNNATC 1 cut(s) 16
BsaJI CCNNGG 2 cut(s) 147, 255
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 511, 614
Bse1I ACTGG 2 cut(s) 50, 317
Bse8I GATNNNNATC 1 cut(s) 16
BseDI CCNNGG 2 cut(s) 147, 255
BseGI GGATG 1 cut(s) 337
BseJI GATNNNNATC 1 cut(s) 16
BseLI CCNNNNNNNGG 3 cut(s) 153, 511, 614
BseMII CTCAG 2 cut(s) 426, 514
BseNI ACTGG 2 cut(s) 50, 317
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 2 cut(s) 264, 591
Bsh1236I CGCG 1 cut(s) 77
BshNI GGYRCC 1 cut(s) 364
BsiHKAI GWGCWC 2 cut(s) 446, 501
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 3 cut(s) 319, 344, 364
BslI CCNNNNNNNGG 3 cut(s) 153, 511, 614
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 3 cut(s) 319, 344, 364
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 3 cut(s) 446, 466, 501
Bsp143I GATC 1 cut(s) 216
Bsp1720I GCTNAGC 1 cut(s) 500
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 1 cut(s) 77
BspCNI CTCAG 2 cut(s) 427, 513
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 3 cut(s) 334, 335, 366
BspMAI CTGCAG 1 cut(s) 282
BspMI ACCTGC 2 cut(s) 481, 527
BspT107I GGYRCC 1 cut(s) 364
BsrI ACTGG 2 cut(s) 50, 317
BssECI CCNNGG 2 cut(s) 147, 255
BssMI GATC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 256, 263
Bst6I CTCTTC 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 718
BstDEI CTNAG 3 cut(s) 220, 435, 500
BstDSI CCRYGG 2 cut(s) 147, 255
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 3 cut(s) 274, 496, 533
BstSCI CCNGG 1 cut(s) 360
BstSFI CTRYAG 1 cut(s) 278
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 264, 591
BtgI CCRYGG 2 cut(s) 147, 255
BtsCI GGATG 1 cut(s) 337
BveI ACCTGC 2 cut(s) 481, 527
Cfr13I GGNCC 3 cut(s) 259, 333, 431
CspCI CAANNNNNGTGG 2 cut(s) 435, 470
CviAII CATG 2 cut(s) 148, 317
DdeI CTNAG 3 cut(s) 220, 435, 500
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
Ecl136II GAGCTC 2 cut(s) 444, 499
Eco130I CCWWGG 1 cut(s) 147
Eco24I GRGCYC 3 cut(s) 446, 466, 501
Eco47I GGWCC 3 cut(s) 259, 333, 431
Eco53kI GAGCTC 2 cut(s) 444, 499
Eco57I CTGAAG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 272
EcoICRI GAGCTC 2 cut(s) 444, 499
EcoO109I RGGNCCY 2 cut(s) 333, 431
EcoT14I CCWWGG 1 cut(s) 147
EcoT38I GRGCYC 3 cut(s) 446, 466, 501
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 2 cut(s) 151, 320
FaiI YATR 9 cut(s) 17, 135, 149, 310, 318, 350, 560, 651, 708
FalI AAGNNNNNCTT 2 cut(s) 613, 645
FaqI GGGAC 3 cut(s) 319, 344, 364
FatI CATG 2 cut(s) 147, 316
Fnu4HI GCNGC 2 cut(s) 278, 605
FokI GGATG 1 cut(s) 324
FriOI GRGCYC 3 cut(s) 446, 466, 501
Fsp4HI GCNGC 2 cut(s) 278, 605
FspBI CTAG 4 cut(s) 153, 459, 569, 642
GluI GCNGC 2 cut(s) 278, 605
GsuI CTGGAG 1 cut(s) 249
HapII CCGG 1 cut(s) 362
Hin1II CATG 2 cut(s) 151, 320
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 362
HphI GGTGA 4 cut(s) 286, 551, 607, 673
Hpy166II GTNNAC 1 cut(s) 259
Hpy188III TCNNGA 3 cut(s) 228, 434, 508
Hpy8I GTNNAC 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 42, 422
HpyCH4III ACNGT 2 cut(s) 256, 263
HpyCH4IV ACGT 2 cut(s) 300, 717
HpyCH4V TGCA 5 cut(s) 280, 419, 485, 536, 724
HpyF10VI GCNNNNNNNGC 3 cut(s) 274, 496, 533
HpyF3I CTNAG 3 cut(s) 220, 435, 500
HpySE526I ACGT 2 cut(s) 300, 717
Hsp92II CATG 2 cut(s) 151, 320
KflI GGGWCCC 1 cut(s) 333
Kzo9I GATC 1 cut(s) 216
LmnI GCTCC 2 cut(s) 496, 601
Lsp1109I GCAGC 2 cut(s) 264, 591
LweI GCATC 2 cut(s) 113, 682
MaeI CTAG 4 cut(s) 153, 459, 569, 642
MaeII ACGT 2 cut(s) 300, 717
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 2 cut(s) 195, 585
MhlI GDGCHC 3 cut(s) 446, 466, 501
MluCI AATT 8 cut(s) 27, 179, 186, 235, 388, 449, 577, 656
MnlI CCTC 8 cut(s) 206, 209, 346, 352, 379, 515, 591, 694
MroXI GAANNNNTTC 1 cut(s) 577
MseI TTAA 5 cut(s) 72, 128, 546, 587, 675
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 362
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 3 cut(s) 274, 496, 533
NciI CCSGG 1 cut(s) 362
NcoI CCATGG 1 cut(s) 147
NdeII GATC 1 cut(s) 216
NlaIII CATG 2 cut(s) 151, 320
NlaIV GGNNCC 3 cut(s) 334, 335, 366
PaqCI CACCTGC 2 cut(s) 481, 527
PdmI GAANNNNTTC 1 cut(s) 577
PkrI GCNGC 2 cut(s) 279, 606
Ppu21I YACGTR 1 cut(s) 718
PpuMI RGGWCCY 2 cut(s) 333, 431
Psp124BI GAGCTC 2 cut(s) 446, 501
Psp5II RGGWCCY 2 cut(s) 333, 431
PspN4I GGNNCC 3 cut(s) 334, 335, 366
PspPI GGNCC 3 cut(s) 259, 333, 431
PspPPI RGGWCCY 2 cut(s) 333, 431
PstI CTGCAG 1 cut(s) 282
SacI GAGCTC 2 cut(s) 446, 501
SaqAI TTAA 5 cut(s) 72, 128, 546, 587, 675
SatI GCNGC 2 cut(s) 278, 605
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 3 cut(s) 259, 333, 431
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 3 cut(s) 446, 466, 501
SfaNI GCATC 2 cut(s) 113, 682
SfcI CTRYAG 1 cut(s) 278
SinI GGWCC 3 cut(s) 259, 333, 431
SmlI CTYRAG 2 cut(s) 465, 685
SmoI CTYRAG 2 cut(s) 465, 685
Sse9I AATT 8 cut(s) 27, 179, 186, 235, 388, 449, 577, 656
SsiI CCGC 1 cut(s) 77
SspI AATATT 2 cut(s) 585, 712
SspMI CTAG 4 cut(s) 153, 459, 569, 642
SstI GAGCTC 2 cut(s) 446, 501
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 2 cut(s) 256, 263
TaiI ACGT 2 cut(s) 303, 720
TasI AATT 8 cut(s) 27, 179, 186, 235, 388, 449, 577, 656
Tru1I TTAA 5 cut(s) 72, 128, 546, 587, 675
Tru9I TTAA 5 cut(s) 72, 128, 546, 587, 675
TseI GCWGC 2 cut(s) 277, 604
TspGWI ACGGA 1 cut(s) 492
VpaK11BI GGWCC 3 cut(s) 259, 333, 431
XapI RAATTY 3 cut(s) 235, 388, 577
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 389
XmnI GAANNNNTTC 1 cut(s) 577
XspI CTAG 4 cut(s) 153, 459, 569, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.