FvH4_3g44790

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000726
Physical Location & Seq
Reverse (-)
37269834 .. 37271167
1334 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44790.t1

Sequence Viewer

Length: 585 bp
ATGTCCAGAATACCTCACATCTTTGCTACACCACTCATAGTCGTCTCCCTTCTTTCTTCATTTGCCCTCATTCTGGTCCAGGGAGAAGATGATCACGATTTCGTGAGAGCCATGGACCGCGATCTCTTGGGTCTCAAGAAAGAAAAGCTCAGCCACTTCCGGTTGTACTGGCATGACATTGCCAGCGGCAAAAACCCAAGCTCAGTCCCAGTGGTGAATTCACCCGGTAACTCATCAACGTTCTTCGGAATGCTGATAATGATCGACGATCCCCTCACCGAAAAGCCGGAGCTGAGCTCGAAACTGCTGGGGAGGGCTCAAGGGTTCTACGCATCAGCTTCACAAAAGGAGTTGGGACTGTTGATGGCCATGAACTTTCATTTTATCAAGGGCAAGTATAATGGGAGCACCATAACTGTGTTGGGGAGGAACCAGGTGTTCAACAAGGTGAGAGAGATGCCGGTGATCGGAGGAAGCGGGCTTTTCAGGTTTGCAAGAGGTTATGTGGAAGCAAGAACTCATACATTTAATATGAGCACCGGAGATGCCATTGTTGAGTACAACTGCTATGTGCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

195

Amino Acids

21.68

Weight (kDa)

8.99

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 191 1.4e-58 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 120
AciI CCGC 3 cut(s) 118, 186, 477
AclI AACGTT 1 cut(s) 239
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 217
AfaI GTAC 2 cut(s) 167, 560
AfiI CCNNNNNNNGG 2 cut(s) 73, 467
AgsI TTSAA 1 cut(s) 442
AjnI CCWGG 2 cut(s) 78, 432
AluBI AGCT 5 cut(s) 148, 201, 292, 297, 338
AluI AGCT 5 cut(s) 148, 201, 292, 297, 338
Alw21I GWGCWC 4 cut(s) 299, 410, 539, 576
Alw26I GTCTC 2 cut(s) 49, 137
AlwI GGATC 1 cut(s) 263
AoxI GGCC 1 cut(s) 366
ApoI RAATTY 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 76, 115
AsuC2I CCSGG 1 cut(s) 225
AsuHPI GGTGA 5 cut(s) 213, 226, 268, 460, 475
AvaII GGWCC 2 cut(s) 76, 115
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 2 cut(s) 299, 319
Bbv12I GWGCWC 4 cut(s) 299, 410, 539, 576
BccI CCATC 1 cut(s) 358
BciT130I CCWGG 2 cut(s) 80, 434
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 2 cut(s) 49, 137
BisI GCNGC 1 cut(s) 187
BlpI GCTNAGC 2 cut(s) 149, 293
BlsI GCNGC 1 cut(s) 188
Bme1390I CCNGG 3 cut(s) 80, 225, 434
Bme18I GGWCC 2 cut(s) 76, 115
BmgT120I GGNCC 2 cut(s) 76, 115
BmiI GGNNCC 1 cut(s) 431
BmrFI CCNGG 3 cut(s) 80, 225, 434
BmrI ACTGGG 1 cut(s) 203
BmsI GCATC 3 cut(s) 341, 447, 535
BmuI ACTGGG 1 cut(s) 203
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 2 cut(s) 149, 293
BpuEI CTTGAG 2 cut(s) 119, 303
BpuMI CCSGG 1 cut(s) 225
BsaBI GATNNNNATC 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 79, 111
BsaWI WCCGGW 2 cut(s) 159, 539
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 467
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 173, 209
Bse3DI GCAATG 1 cut(s) 177
Bse8I GATNNNNATC 1 cut(s) 260
BseBI CCWGG 2 cut(s) 80, 434
BseDI CCNNGG 2 cut(s) 79, 111
BseJI GATNNNNATC 1 cut(s) 260
BseLI CCNNNNNNNGG 2 cut(s) 73, 467
BseMI GCAATG 1 cut(s) 177
BseMII CTCAG 3 cut(s) 163, 216, 284
BseNI ACTGG 2 cut(s) 173, 209
BseYI CCCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 368
BsiHKAI GWGCWC 4 cut(s) 299, 410, 539, 576
BsiSI CCGG 5 cut(s) 160, 225, 287, 461, 540
BslFI GGGAC 2 cut(s) 191, 369
BslI CCNNNNNNNGG 2 cut(s) 73, 467
BsmAI GTCTC 2 cut(s) 49, 137
BsmBI CGTCTC 1 cut(s) 49
BsmFI GGGAC 2 cut(s) 191, 369
BsmI GAATGC 1 cut(s) 255
BsnI GGCC 1 cut(s) 368
Bso31I GGTCTC 1 cut(s) 137
Bsp1286I GDGCHC 5 cut(s) 299, 319, 410, 539, 576
Bsp143I GATC 5 cut(s) 91, 121, 261, 268, 465
Bsp1720I GCTNAGC 2 cut(s) 149, 293
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 3 cut(s) 118, 186, 477
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 3 cut(s) 162, 215, 285
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 431
BspPI GGATC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 177
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 2 cut(s) 173, 209
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 79, 111
BssMI GATC 5 cut(s) 91, 121, 261, 268, 465
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 2 cut(s) 80, 434
Bst4CI ACNGT 2 cut(s) 360, 418
BstC8I GCNNGC 2 cut(s) 184, 479
BstDEI CTNAG 3 cut(s) 149, 202, 293
BstDSI CCRYGG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 5 cut(s) 94, 124, 264, 271, 468
BstMAI GTCTC 2 cut(s) 49, 137
BstMBI GATC 5 cut(s) 91, 121, 261, 268, 465
BstNI CCWGG 2 cut(s) 80, 434
BstSCI CCNGG 3 cut(s) 78, 223, 432
BstUI CGCG 1 cut(s) 120
BsuRI GGCC 1 cut(s) 368
BtgI CCRYGG 1 cut(s) 111
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 184, 479
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 76, 115
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 2 cut(s) 166, 559
CviAII CATG 3 cut(s) 112, 173, 370
CviQI GTAC 2 cut(s) 166, 559
DdeI CTNAG 3 cut(s) 149, 202, 293
DpnI GATC 5 cut(s) 93, 123, 263, 270, 467
DpnII GATC 5 cut(s) 91, 121, 261, 268, 465
EaeI YGGCCR 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 2 cut(s) 299, 319
Eco31I GGTCTC 1 cut(s) 137
Eco47I GGWCC 2 cut(s) 76, 115
Eco53kI GAGCTC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 217
EcoRII CCWGG 2 cut(s) 78, 432
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 2 cut(s) 299, 319
ErhI CCWWGG 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 3 cut(s) 115, 176, 373
FaqI GGGAC 2 cut(s) 191, 369
FatI CATG 3 cut(s) 111, 172, 369
FauI CCCGC 1 cut(s) 470
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 1 cut(s) 187
FriOI GRGCYC 2 cut(s) 299, 319
Fsp4HI GCNGC 1 cut(s) 187
GluI GCNGC 1 cut(s) 187
GsaI CCCAGC 1 cut(s) 311
HaeIII GGCC 1 cut(s) 368
HapII CCGG 5 cut(s) 160, 225, 287, 461, 540
Hin1II CATG 3 cut(s) 115, 176, 373
HpaII CCGG 5 cut(s) 160, 225, 287, 461, 540
HphI GGTGA 5 cut(s) 213, 226, 268, 460, 475
Hpy188I TCNGA 2 cut(s) 248, 470
Hpy188III TCNNGA 4 cut(s) 6, 95, 103, 136
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 360, 418
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 1 cut(s) 494
HpyF3I CTNAG 3 cut(s) 149, 202, 293
HpySE526I ACGT 1 cut(s) 239
Hsp92II CATG 3 cut(s) 115, 176, 373
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 5 cut(s) 91, 121, 261, 268, 465
LmnI GCTCC 3 cut(s) 289, 405, 579
LweI GCATC 3 cut(s) 341, 447, 535
MabI ACCWGGT 1 cut(s) 432
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 227
MalI GATC 5 cut(s) 93, 123, 263, 270, 467
MboI GATC 5 cut(s) 91, 121, 261, 268, 465
MboII GAAGA 3 cut(s) 48, 98, 235
MhlI GDGCHC 5 cut(s) 299, 319, 410, 539, 576
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 1 cut(s) 217
MluNI TGGCCA 1 cut(s) 368
MnlI CCTC 7 cut(s) 24, 77, 284, 306, 420, 464, 491
Mox20I TGGCCA 1 cut(s) 368
MscI TGGCCA 1 cut(s) 368
MseI TTAA 1 cut(s) 528
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 186
MspI CCGG 5 cut(s) 160, 225, 287, 461, 540
MspR9I CCNGG 3 cut(s) 80, 225, 434
Mva1269I GAATGC 1 cut(s) 255
MvaI CCWGG 2 cut(s) 80, 434
MvnI CGCG 1 cut(s) 120
NciI CCSGG 1 cut(s) 225
NcoI CCATGG 1 cut(s) 111
NdeII GATC 5 cut(s) 91, 121, 261, 268, 465
NlaIII CATG 3 cut(s) 115, 176, 373
NlaIV GGNNCC 1 cut(s) 431
PctI GAATGC 1 cut(s) 255
PkrI GCNGC 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 299
Psp1406I AACGTT 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 78, 432
PspFI CCCAGC 1 cut(s) 307
PspGI CCWGG 2 cut(s) 78, 432
PspN4I GGNNCC 1 cut(s) 431
PspPI GGNCC 2 cut(s) 76, 115
RsaI GTAC 2 cut(s) 167, 560
RsaNI GTAC 2 cut(s) 166, 559
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 299
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 5 cut(s) 91, 121, 261, 268, 465
Sau96I GGNCC 2 cut(s) 76, 115
ScrFI CCNGG 3 cut(s) 80, 225, 434
SduI GDGCHC 5 cut(s) 299, 319, 410, 539, 576
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 3 cut(s) 341, 447, 535
SinI GGWCC 2 cut(s) 76, 115
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 134, 318
SmoI CTYRAG 2 cut(s) 134, 318
Sse9I AATT 1 cut(s) 217
SsiI CCGC 3 cut(s) 118, 186, 477
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 3 cut(s) 78, 223, 432
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 360, 418
TaiI ACGT 1 cut(s) 242
TaqI TCGA 2 cut(s) 264, 299
TasI AATT 1 cut(s) 217
TatI WGTACW 2 cut(s) 165, 558
TauI GCSGC 1 cut(s) 189
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 1 cut(s) 216
TspDTI ATGAA 3 cut(s) 48, 368, 386
TspRI CASTG 1 cut(s) 216
VpaK11BI GGWCC 2 cut(s) 76, 115
XapI RAATTY 1 cut(s) 217
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.