Rh5DG562500

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000726
Physical Location & Seq
Reverse (-)
86695832 .. 86708025
12194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG562500.1

Sequence Viewer

Length: 669 bp
ATGTTGAGGTCAAGCAGTATGACTACTCAATCATTTCACTATATATGTGGACACAACCCTCTCAATTGGAAAAAGGTTCCAACAATGGCCAGAATATTTCCCATCTTTGCTACACAGTTCATAGTTCTCTCCCTTCTTTCTTCCTTTGCTCTAATTCTGGTCCAGGGAGAAGATGATCACGATTTCGTGAAAGCCATGGACCGAGATCTCTTGGGGCTCAAGAAAGAAAAGTTCAGCCACTTCAAGTTGTACTGGCATGACATTCAGAGCGGCAAAAACCCAAGCTCAATCGGAGTGGTGAAATCACCCGTCAACTCATCGACGTTCTTCGGATTGGTGAACATGATCGACAATCCCCTCACCGAAAAGCCGGAGCTGAGCTCGAAGCTACTGGGGAGGGCTCAAGGGTTCTACGCATCAGCTTCACAACAGGAGGTCGGGCTGTTGATGGCTATGAACTTTCATTTTATTCAGGGCAAGTACAACGGGAGCACCATAACCGTGCTGGGGAGGAACCCAGTGTTCAACAAGGTGAGGGAGATGCCGGTGATCGGAGGAAGTGGGCTTTTCAGGTTTGCAAGAGGTTATGTTGAAGCAAGAACTCATACATTCACTGCGAGCACCGGAGATGCCATCGTTGAGTACAATGCCTATGTGCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

222

Amino Acids

24.88

Weight (kDa)

9.41

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 79 - 219 7.6e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 270
AciI CCGC 1 cut(s) 270
AcoI YGGCCR 1 cut(s) 87
AfaI GTAC 3 cut(s) 251, 482, 644
AfiI CCNNNNNNNGG 2 cut(s) 507, 551
AgsI TTSAA 3 cut(s) 244, 526, 593
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 5 cut(s) 285, 376, 381, 388, 422
AluI AGCT 5 cut(s) 285, 376, 381, 388, 422
Alw21I GWGCWC 4 cut(s) 383, 494, 623, 660
AoxI GGCC 1 cut(s) 87
AspS9I GGNCC 2 cut(s) 160, 199
AsuHPI GGTGA 6 cut(s) 297, 310, 349, 352, 544, 559
AvaII GGWCC 2 cut(s) 160, 199
BalI TGGCCA 1 cut(s) 89
BanII GRGCYC 3 cut(s) 219, 383, 403
Bbv12I GWGCWC 4 cut(s) 383, 494, 623, 660
BccI CCATC 3 cut(s) 110, 442, 641
BciT130I CCWGG 1 cut(s) 164
BclI TGATCA 1 cut(s) 175
BglII AGATCT 1 cut(s) 205
BisI GCNGC 1 cut(s) 271
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 2 cut(s) 160, 199
BmgT120I GGNCC 2 cut(s) 160, 199
BmiI GGNNCC 2 cut(s) 78, 515
BmrFI CCNGG 1 cut(s) 164
BmrI ACTGGG 2 cut(s) 401, 512
BmsI GCATC 3 cut(s) 425, 531, 619
BmuI ACTGGG 2 cut(s) 401, 512
BplI GAGNNNNNCTC 2 cut(s) 365, 397
Bpu1102I GCTNAGC 1 cut(s) 377
BpuEI CTTGAG 2 cut(s) 203, 387
BsaJI CCNNGG 2 cut(s) 163, 195
BsaWI WCCGGW 1 cut(s) 623
Bsc4I CCNNNNNNNGG 2 cut(s) 507, 551
Bse118I RCCGGY 1 cut(s) 544
Bse1I ACTGG 3 cut(s) 257, 396, 518
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 163, 195
BseLI CCNNNNNNNGG 2 cut(s) 507, 551
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 3 cut(s) 257, 396, 518
BseYI CCCAGC 1 cut(s) 505
BshFI GGCC 1 cut(s) 89
BsiHKAI GWGCWC 4 cut(s) 383, 494, 623, 660
BsiSI CCGG 3 cut(s) 371, 545, 624
BslI CCNNNNNNNGG 2 cut(s) 507, 551
BsnI GGCC 1 cut(s) 89
Bsp1286I GDGCHC 6 cut(s) 219, 383, 403, 494, 623, 660
Bsp143I GATC 4 cut(s) 175, 205, 345, 549
Bsp1720I GCTNAGC 1 cut(s) 377
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 1 cut(s) 270
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 78, 515
BsrBI CCGCTC 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 544
BsrI ACTGG 3 cut(s) 257, 396, 518
BssAI RCCGGY 1 cut(s) 544
BssECI CCNNGG 2 cut(s) 163, 195
BssMI GATC 4 cut(s) 175, 205, 345, 549
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 117, 502
BstC8I GCNNGC 1 cut(s) 619
BstDEI CTNAG 1 cut(s) 377
BstDSI CCRYGG 1 cut(s) 195
BstKTI GATC 4 cut(s) 178, 208, 348, 552
BstMBI GATC 4 cut(s) 175, 205, 345, 549
BstNI CCWGG 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 195
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 2 cut(s) 525, 612
Cac8I GCNNGC 1 cut(s) 619
Cfr10I RCCGGY 1 cut(s) 544
Cfr13I GGNCC 2 cut(s) 160, 199
Csp6I GTAC 3 cut(s) 250, 481, 643
CspCI CAANNNNNGTGG 2 cut(s) 276, 311
CviAII CATG 3 cut(s) 196, 257, 343
CviQI GTAC 3 cut(s) 250, 481, 643
DdeI CTNAG 1 cut(s) 377
DpnI GATC 4 cut(s) 177, 207, 347, 551
DpnII GATC 4 cut(s) 175, 205, 345, 549
EaeI YGGCCR 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 3 cut(s) 219, 383, 403
Eco47I GGWCC 2 cut(s) 160, 199
Eco53kI GAGCTC 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 3 cut(s) 219, 383, 403
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 3 cut(s) 199, 260, 346
FatI CATG 3 cut(s) 195, 256, 342
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 1 cut(s) 271
FriOI GRGCYC 3 cut(s) 219, 383, 403
Fsp4HI GCNGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 271
GsaI CCCAGC 1 cut(s) 509
HaeIII GGCC 1 cut(s) 89
HapII CCGG 3 cut(s) 371, 545, 624
Hin1II CATG 3 cut(s) 199, 260, 346
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HpaII CCGG 3 cut(s) 371, 545, 624
HphI GGTGA 6 cut(s) 297, 310, 349, 352, 544, 559
Hpy166II GTNNAC 3 cut(s) 50, 313, 340
Hpy188I TCNGA 4 cut(s) 267, 293, 332, 554
Hpy188III TCNNGA 3 cut(s) 179, 187, 220
Hpy8I GTNNAC 3 cut(s) 50, 313, 340
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 117, 502
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 1 cut(s) 578
HpyF3I CTNAG 1 cut(s) 377
HpySE526I ACGT 1 cut(s) 323
Hsp92II CATG 3 cut(s) 199, 260, 346
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 4 cut(s) 175, 205, 345, 549
LmnI GCTCC 3 cut(s) 373, 489, 663
LweI GCATC 3 cut(s) 425, 531, 619
MaeII ACGT 1 cut(s) 323
MalI GATC 4 cut(s) 177, 207, 347, 551
MbiI CCGCTC 1 cut(s) 270
MboI GATC 4 cut(s) 175, 205, 345, 549
MboII GAAGA 3 cut(s) 132, 182, 319
MfeI CAATTG 1 cut(s) 64
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 6 cut(s) 219, 383, 403, 494, 623, 660
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 2 cut(s) 64, 153
MluNI TGGCCA 1 cut(s) 89
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 8 cut(s) 69, 368, 390, 427, 504, 528, 548, 575
Mox20I TGGCCA 1 cut(s) 89
MscI TGGCCA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 500
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 3 cut(s) 371, 545, 624
MspR9I CCNGG 1 cut(s) 164
MunI CAATTG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 164
NcoI CCATGG 1 cut(s) 195
NdeII GATC 4 cut(s) 175, 205, 345, 549
NlaIII CATG 3 cut(s) 199, 260, 346
NlaIV GGNNCC 2 cut(s) 78, 515
PkrI GCNGC 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 162
PspFI CCCAGC 1 cut(s) 505
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 78, 515
PspPI GGNCC 2 cut(s) 160, 199
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 3 cut(s) 251, 482, 644
RsaNI GTAC 3 cut(s) 250, 481, 643
RseI CAYNNNNRTG 1 cut(s) 500
SacI GAGCTC 1 cut(s) 383
SatI GCNGC 1 cut(s) 271
Sau3AI GATC 4 cut(s) 175, 205, 345, 549
Sau96I GGNCC 2 cut(s) 160, 199
ScrFI CCNGG 1 cut(s) 164
SduI GDGCHC 6 cut(s) 219, 383, 403, 494, 623, 660
SfaNI GCATC 3 cut(s) 425, 531, 619
SinI GGWCC 2 cut(s) 160, 199
SmiMI CAYNNNNRTG 1 cut(s) 500
SmlI CTYRAG 2 cut(s) 218, 402
SmoI CTYRAG 2 cut(s) 218, 402
Sse9I AATT 2 cut(s) 64, 153
SsiI CCGC 1 cut(s) 270
SspI AATATT 1 cut(s) 96
SstI GAGCTC 1 cut(s) 383
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 117, 502
TaiI ACGT 1 cut(s) 326
TaqI TCGA 3 cut(s) 320, 348, 383
TaqII GACCGA 1 cut(s) 216
TasI AATT 2 cut(s) 64, 153
TatI WGTACW 3 cut(s) 249, 480, 642
TauI GCSGC 1 cut(s) 273
TscAI CASTG 2 cut(s) 525, 619
TspDTI ATGAA 3 cut(s) 109, 452, 470
TspRI CASTG 2 cut(s) 525, 619
VpaK11BI GGWCC 2 cut(s) 160, 199
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.