MD03G1010600.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000726
Physical Location & Seq
Forward (+)
809715 .. 810284
570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1010600.v1.1.491

Sequence Viewer

Length: 570 bp
ATGACCAAAATTCCTCCCCTCTTAGCTACCCAAGTCATAGTGCTTCTTTCTTCATTTTCCATCATTTCGGTCCAAGGAAAAAACCCAGGTGTTGTTAGAACAAAGCTTTTGGGGCTGAAAAAAGAAGAAAAACTTAGCCACTTTCGGTTGTATTGGCACGACATTGTTAGCGGTCCAAATCCCAGTTCAGTTGCAGTGGTGAAACCAGTCACGAATTCATCAACAGGGTTTGGTTTTATAAGCATGATTGATGATCCACTTACTGAAGGACCTGAAATGAGCTCAAAATTGTTGGGAAGAGCTCAAGGGTTTTATGGATCTGCTTCACAAAAAGAGGCTGGCCTATTGATGGCCATGAATTTTGCTTTCATTCAAGGCAAGTATAATGGTAGCACCATAACTGTGCTAGGAAGGAGCCAAGTATTTAACAAGGTCAGGGAGATGCCTGTGATCGGAGGCAGCGGGCTTTTCCGGTTTGCGAGGGGTTATGCTGAAGCAAGAACTCACTATTTCAACCTCACTACTGGGGATGCTATAGTTGAGTATAATGTCTATGTGCTGCATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

190

Amino Acids

20.68

Weight (kDa)

9.66

Isoelectric Point (pI)

28.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 46 - 186 3e-58 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 239
AciI CCGC 2 cut(s) 171, 462
AclWI GGATC 2 cut(s) 248, 325
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 3 cut(s) 9, 214, 358
AcuI CTGAAG 2 cut(s) 285, 513
AfiI CCNNNNNNNGG 2 cut(s) 349, 452
AgsI TTSAA 2 cut(s) 374, 514
AjnI CCWGG 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 169, 201
AluBI AGCT 4 cut(s) 26, 106, 282, 302
AluI AGCT 4 cut(s) 26, 106, 282, 302
Alw21I GWGCWC 2 cut(s) 284, 304
AlwI GGATC 2 cut(s) 248, 325
AoxI GGCC 2 cut(s) 340, 351
ApeKI GCWGC 2 cut(s) 459, 559
ApoI RAATTY 3 cut(s) 9, 214, 358
AspS9I GGNCC 3 cut(s) 70, 173, 269
AsuHPI GGTGA 1 cut(s) 211
AvaII GGWCC 3 cut(s) 70, 173, 269
BalI TGGCCA 1 cut(s) 353
BanII GRGCYC 2 cut(s) 284, 304
Bbv12I GWGCWC 2 cut(s) 284, 304
BbvI GCAGC 2 cut(s) 471, 546
BccI CCATC 2 cut(s) 68, 343
BciT130I CCWGG 1 cut(s) 87
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 1 cut(s) 534
BisI GCNGC 2 cut(s) 460, 560
BlsI GCNGC 2 cut(s) 461, 561
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 3 cut(s) 70, 173, 269
BmgT120I GGNCC 3 cut(s) 70, 173, 269
BmiI GGNNCC 1 cut(s) 416
BmrFI CCNGG 1 cut(s) 87
BmrI ACTGGG 2 cut(s) 177, 534
BmsI GCATC 2 cut(s) 432, 520
BmuI ACTGGG 2 cut(s) 177, 534
BpuEI CTTGAG 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 73, 85
BsaWI WCCGGW 1 cut(s) 471
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 452
Bse1I ACTGG 3 cut(s) 183, 206, 529
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 73, 85
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 2 cut(s) 349, 452
BseNI ACTGG 3 cut(s) 183, 206, 529
BseXI GCAGC 2 cut(s) 471, 546
BshFI GGCC 2 cut(s) 342, 353
BsiHKAI GWGCWC 2 cut(s) 284, 304
BsiSI CCGG 1 cut(s) 472
BslI CCNNNNNNNGG 2 cut(s) 349, 452
BsnI GGCC 2 cut(s) 342, 353
Bsp1286I GDGCHC 2 cut(s) 284, 304
Bsp143I GATC 3 cut(s) 253, 317, 450
BspACI CCGC 2 cut(s) 171, 462
BspANI GGCC 2 cut(s) 342, 353
BspLI GGNNCC 1 cut(s) 416
BspPI GGATC 2 cut(s) 248, 325
BspQI GCTCTTC 1 cut(s) 292
BsrI ACTGG 3 cut(s) 183, 206, 529
BssECI CCNNGG 2 cut(s) 73, 85
BssMI GATC 3 cut(s) 253, 317, 450
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 340, 464
BstDEI CTNAG 2 cut(s) 22, 134
BstF5I GGATG 1 cut(s) 535
BstKTI GATC 3 cut(s) 256, 320, 453
BstMBI GATC 3 cut(s) 253, 317, 450
BstMWI GCNNNNNNNGC 1 cut(s) 112
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 534
BstV1I GCAGC 2 cut(s) 471, 546
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 2 cut(s) 342, 353
BtsCI GGATG 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 2 cut(s) 340, 464
Cfr13I GGNCC 3 cut(s) 70, 173, 269
CviAII CATG 2 cut(s) 244, 355
DdeI CTNAG 2 cut(s) 22, 134
DpnI GATC 3 cut(s) 255, 319, 452
DpnII GATC 3 cut(s) 253, 317, 450
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
Ecl136II GAGCTC 2 cut(s) 282, 302
Eco130I CCWWGG 1 cut(s) 73
Eco24I GRGCYC 2 cut(s) 284, 304
Eco47I GGWCC 3 cut(s) 70, 173, 269
Eco53kI GAGCTC 2 cut(s) 282, 302
Eco57I CTGAAG 2 cut(s) 285, 513
EcoICRI GAGCTC 2 cut(s) 282, 302
EcoO109I RGGNCCY 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 73
EcoT38I GRGCYC 2 cut(s) 284, 304
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 2 cut(s) 247, 358
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FatI CATG 2 cut(s) 243, 354
FauI CCCGC 1 cut(s) 455
Fnu4HI GCNGC 2 cut(s) 460, 560
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 2 cut(s) 284, 304
Fsp4HI GCNGC 2 cut(s) 460, 560
FspBI CTAG 1 cut(s) 407
GluI GCNGC 2 cut(s) 460, 560
HaeIII GGCC 2 cut(s) 342, 353
HapII CCGG 1 cut(s) 472
Hin1II CATG 2 cut(s) 247, 358
HindIII AAGCTT 1 cut(s) 104
HpaII CCGG 1 cut(s) 472
HphI GGTGA 1 cut(s) 211
Hpy188I TCNGA 1 cut(s) 455
Hpy188III TCNNGA 1 cut(s) 211
HpyAV CCTTC 2 cut(s) 260, 405
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4V TGCA 2 cut(s) 194, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 112
HpyF3I CTNAG 2 cut(s) 22, 134
Hsp92II CATG 2 cut(s) 247, 358
Kzo9I GATC 3 cut(s) 253, 317, 450
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 414
Lsp1109I GCAGC 2 cut(s) 471, 546
LweI GCATC 2 cut(s) 432, 520
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 208
MalI GATC 3 cut(s) 255, 319, 452
MboI GATC 3 cut(s) 253, 317, 450
MboII GAAGA 3 cut(s) 42, 137, 309
MflI RGATCY 1 cut(s) 317
MhlI GDGCHC 2 cut(s) 284, 304
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 4 cut(s) 9, 214, 287, 358
MluNI TGGCCA 1 cut(s) 353
MnlI CCTC 6 cut(s) 24, 29, 328, 449, 474, 527
Mox20I TGGCCA 1 cut(s) 353
MscI TGGCCA 1 cut(s) 353
MseI TTAA 1 cut(s) 426
MslI CAYNNNNRTG 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 462
MspI CCGG 1 cut(s) 472
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 112
NdeII GATC 3 cut(s) 253, 317, 450
NlaIII CATG 2 cut(s) 247, 358
NlaIV GGNNCC 1 cut(s) 416
NmuCI GTSAC 1 cut(s) 208
PciSI GCTCTTC 1 cut(s) 292
PkrI GCNGC 2 cut(s) 461, 561
PpuMI RGGWCCY 1 cut(s) 269
PsiI TTATAA 1 cut(s) 239
Psp124BI GAGCTC 2 cut(s) 284, 304
Psp5II RGGWCCY 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 416
PspPI GGNCC 3 cut(s) 70, 173, 269
PspPPI RGGWCCY 1 cut(s) 269
PsuI RGATCY 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 401
SacI GAGCTC 2 cut(s) 284, 304
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 2 cut(s) 460, 560
Sau3AI GATC 3 cut(s) 253, 317, 450
Sau96I GGNCC 3 cut(s) 70, 173, 269
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 2 cut(s) 284, 304
SetI ASST 8 cut(s) 28, 91, 108, 274, 284, 304, 435, 519
SfaNI GCATC 2 cut(s) 432, 520
SfcI CTRYAG 1 cut(s) 534
SinI GGWCC 3 cut(s) 70, 173, 269
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
Sse9I AATT 4 cut(s) 9, 214, 287, 358
SsiI CCGC 2 cut(s) 171, 462
SspMI CTAG 1 cut(s) 407
SstI GAGCTC 2 cut(s) 284, 304
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 403
TaqII GACCGA 1 cut(s) 58
TasI AATT 4 cut(s) 9, 214, 287, 358
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 1 cut(s) 201
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 2 cut(s) 459, 559
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 4 cut(s) 42, 207, 358, 371
TspRI CASTG 1 cut(s) 201
VpaK11BI GGWCC 3 cut(s) 70, 173, 269
XapI RAATTY 3 cut(s) 9, 214, 358
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.