FvH4_3g44800

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000726
Physical Location & Seq
Reverse (-)
37283524 .. 37284267
744 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44800.t1

Sequence Viewer

Length: 585 bp
ATGGCCAGAATACCTCCCATGTTTGCTACACAACTCATACTTCTCTCCCTTCTTTCTTCCTTTGCCCTCATTCTGGTCCGGGGAGAAGATGATCACGATTTCGTGACAGCCATGGACCGCGATCTCTTGGGGCTCAAGAAAGAAAAGCTCAGTCACTTCCGGTTGTACTGGCATGACATTGTGGCCGGCAAAAACCCAAGCTCAATCGGAGTGGTGAAATCACCCGGTAACTCATCAACATTCTTCGGAGCCGTGAGCATGATTGACAATCCCCTCACCGAAAAGCCGGAGCTGAGCTCGAAGCTACTAGGGAGGGCTCAAGGGTTCTATGCATCAGCTGCACAACAGGAAATCGGGCTGTTGATGGCCATGAACTTTCATTTTACTCAGGGCAAATATAATGGAAGCACCATAACTGTGCTGGGGAGGAACTCGGCGCTCAACAAGGTGAGAGAGATGCCGGTGATCGGAGGAAGCGGGCTTTTCAGGTTTGCAAGAGGTTATGTTGAAGCAAGAACTCACACATTCACTCCGAGCACCGGAGATGCCATCGTGGAGTACAATTGCTATGTGCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

195

Amino Acids

21.4

Weight (kDa)

9.01

Isoelectric Point (pI)

28.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 191 6.3e-57 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 120
AciI CCGC 2 cut(s) 118, 477
AcoI YGGCCR 3 cut(s) 3, 183, 366
AfaI GTAC 2 cut(s) 167, 560
AfiI CCNNNNNNNGG 3 cut(s) 73, 467, 539
AgsI TTSAA 1 cut(s) 509
AluBI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
AluI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
Alw21I GWGCWC 3 cut(s) 299, 539, 576
AoxI GGCC 3 cut(s) 3, 183, 366
ApeKI GCWGC 1 cut(s) 338
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 2 cut(s) 76, 115
AsuC2I CCSGG 2 cut(s) 80, 225
AsuHPI GGTGA 5 cut(s) 213, 226, 268, 460, 475
AvaII GGWCC 2 cut(s) 76, 115
BalI TGGCCA 2 cut(s) 5, 368
BanII GRGCYC 3 cut(s) 135, 299, 319
Bbv12I GWGCWC 3 cut(s) 299, 539, 576
BbvI GCAGC 1 cut(s) 325
BccI CCATC 2 cut(s) 358, 557
BceAI ACGGC 1 cut(s) 236
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 2 cut(s) 80, 225
BfaI CTAG 1 cut(s) 308
BfoI RGCGCY 1 cut(s) 440
BisI GCNGC 1 cut(s) 339
BlpI GCTNAGC 1 cut(s) 293
BlsI GCNGC 1 cut(s) 340
Bme1390I CCNGG 2 cut(s) 80, 225
Bme18I GGWCC 2 cut(s) 76, 115
BmgT120I GGNCC 2 cut(s) 76, 115
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 2 cut(s) 80, 225
BmsI GCATC 3 cut(s) 341, 447, 535
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 1 cut(s) 293
BpuEI CTTGAG 2 cut(s) 119, 303
BpuMI CCSGG 2 cut(s) 80, 225
BsaJI CCNNGG 2 cut(s) 79, 111
BsaWI WCCGGW 2 cut(s) 159, 539
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 467, 539
Bse118I RCCGGY 2 cut(s) 185, 460
Bse1I ACTGG 1 cut(s) 173
BseDI CCNNGG 2 cut(s) 79, 111
BseLI CCNNNNNNNGG 3 cut(s) 73, 467, 539
BseMII CTCAG 3 cut(s) 163, 284, 401
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 325
BseYI CCCAGC 1 cut(s) 421
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 5, 185, 368
BsiHKAI GWGCWC 3 cut(s) 299, 539, 576
BsiSI CCGG 7 cut(s) 79, 160, 186, 225, 287, 461, 540
BslI CCNNNNNNNGG 3 cut(s) 73, 467, 539
BsnI GGCC 3 cut(s) 5, 185, 368
Bsp1286I GDGCHC 5 cut(s) 135, 299, 319, 539, 576
Bsp143I GATC 3 cut(s) 91, 121, 465
Bsp1720I GCTNAGC 1 cut(s) 293
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 2 cut(s) 118, 477
BspANI GGCC 3 cut(s) 5, 185, 368
BspCNI CTCAG 3 cut(s) 162, 285, 400
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 250
BsrFI RCCGGY 2 cut(s) 185, 460
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 2 cut(s) 185, 460
BssECI CCNNGG 2 cut(s) 79, 111
BssMI GATC 3 cut(s) 91, 121, 465
BssT1I CCWWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 418
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 187, 479
BstDEI CTNAG 3 cut(s) 149, 293, 387
BstDSI CCRYGG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 3 cut(s) 94, 124, 468
BstMBI GATC 3 cut(s) 91, 121, 465
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstSCI CCNGG 2 cut(s) 78, 223
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 325
BsuRI GGCC 3 cut(s) 5, 185, 368
BtgI CCRYGG 1 cut(s) 111
Cac8I GCNNGC 2 cut(s) 187, 479
CfoI GCGC 1 cut(s) 439
Cfr10I RCCGGY 2 cut(s) 185, 460
Cfr13I GGNCC 2 cut(s) 76, 115
Csp6I GTAC 2 cut(s) 166, 559
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 5 cut(s) 19, 112, 173, 259, 370
CviQI GTAC 2 cut(s) 166, 559
DdeI CTNAG 3 cut(s) 149, 293, 387
DpnI GATC 3 cut(s) 93, 123, 467
DpnII GATC 3 cut(s) 91, 121, 465
EaeI YGGCCR 3 cut(s) 3, 183, 366
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 3 cut(s) 135, 299, 319
Eco47I GGWCC 2 cut(s) 76, 115
Eco53kI GAGCTC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 334
EcoT38I GRGCYC 3 cut(s) 135, 299, 319
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 5 cut(s) 22, 115, 176, 262, 373
FatI CATG 5 cut(s) 18, 111, 172, 258, 369
FauI CCCGC 1 cut(s) 470
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 1 cut(s) 339
FriOI GRGCYC 3 cut(s) 135, 299, 319
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 1 cut(s) 308
GlaI GCGC 1 cut(s) 438
GluI GCNGC 1 cut(s) 339
GsaI CCCAGC 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 3 cut(s) 5, 185, 368
HapII CCGG 7 cut(s) 79, 160, 186, 225, 287, 461, 540
HhaI GCGC 1 cut(s) 439
Hin1II CATG 5 cut(s) 22, 115, 176, 262, 373
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HpaII CCGG 7 cut(s) 79, 160, 186, 225, 287, 461, 540
HphI GGTGA 5 cut(s) 213, 226, 268, 460, 475
Hpy188I TCNGA 4 cut(s) 209, 248, 470, 534
Hpy188III TCNNGA 3 cut(s) 95, 103, 136
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4V TGCA 3 cut(s) 332, 341, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 3 cut(s) 149, 293, 387
Hsp92II CATG 5 cut(s) 22, 115, 176, 262, 373
HspAI GCGC 1 cut(s) 437
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 91, 121, 465
LmnI GCTCC 3 cut(s) 248, 289, 579
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 3 cut(s) 341, 447, 535
MaeI CTAG 1 cut(s) 308
MaeIII GTNAC 3 cut(s) 103, 152, 227
MalI GATC 3 cut(s) 93, 123, 467
MboI GATC 3 cut(s) 91, 121, 465
MboII GAAGA 3 cut(s) 48, 98, 235
MfeI CAATTG 1 cut(s) 562
MhlI GDGCHC 5 cut(s) 135, 299, 319, 539, 576
MlsI TGGCCA 2 cut(s) 5, 368
MluCI AATT 1 cut(s) 562
MluNI TGGCCA 2 cut(s) 5, 368
MnlI CCTC 7 cut(s) 24, 77, 284, 306, 420, 464, 491
Mox20I TGGCCA 2 cut(s) 5, 368
Mph1103I ATGCAT 1 cut(s) 334
MroNI GCCGGC 1 cut(s) 185
MscI TGGCCA 2 cut(s) 5, 368
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 2 cut(s) 5, 368
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 7 cut(s) 79, 160, 186, 225, 287, 461, 540
MspR9I CCNGG 2 cut(s) 80, 225
MunI CAATTG 1 cut(s) 562
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 338
NaeI GCCGGC 1 cut(s) 187
NciI CCSGG 2 cut(s) 80, 225
NcoI CCATGG 1 cut(s) 111
NdeII GATC 3 cut(s) 91, 121, 465
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 5 cut(s) 22, 115, 176, 262, 373
NlaIV GGNNCC 1 cut(s) 250
NmeAIII GCCGAG 1 cut(s) 413
NmuCI GTSAC 2 cut(s) 103, 152
NsiI ATGCAT 1 cut(s) 334
PdiI GCCGGC 1 cut(s) 187
PkrI GCNGC 1 cut(s) 340
Psp124BI GAGCTC 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 421
PspN4I GGNNCC 1 cut(s) 250
PspPI GGNCC 2 cut(s) 76, 115
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 2 cut(s) 167, 560
RsaNI GTAC 2 cut(s) 166, 559
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 299
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 3 cut(s) 91, 121, 465
Sau96I GGNCC 2 cut(s) 76, 115
ScrFI CCNGG 2 cut(s) 80, 225
SduI GDGCHC 5 cut(s) 135, 299, 319, 539, 576
SfaNI GCATC 3 cut(s) 341, 447, 535
SinI GGWCC 2 cut(s) 76, 115
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 134, 318
SmoI CTYRAG 2 cut(s) 134, 318
Sse9I AATT 1 cut(s) 562
SsiI CCGC 2 cut(s) 118, 477
SspMI CTAG 1 cut(s) 308
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 78, 223
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 418
TaqI TCGA 1 cut(s) 299
TasI AATT 1 cut(s) 562
TatI WGTACW 2 cut(s) 165, 558
TseFI GTSAC 2 cut(s) 103, 152
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 2 cut(s) 103, 152
TspDTI ATGAA 2 cut(s) 368, 386
VpaK11BI GGWCC 2 cut(s) 76, 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XspI CTAG 1 cut(s) 308
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.