RchiOBHm_Chr5g0080641

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000726
Physical Location & Seq
Forward (+)
86602851 .. 86603770
920 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35493

Sequence Viewer

Length: 528 bp
ATGGCCAGAATACCTCCCATCTTTGCTACACAGTTCATAGTTCTCTCCCTTCTTTCTTCCTTTGCCCTAATTCTGGTCCAGGGAGAAGATGATCACGATTTCGTGAGAGCCATGGACCGCGATCTCTTGGGGCTCAAGAAAGAAAAGCTCAGCCACTTCAAGTTGTACTGGCATGACATTTTGAGCGGCAAAAACCCAAGCTCAATCGGAGTGGTGAAATCACCCGTTAACTCATCAACGCTCTTCGGATTCGTGAACATGATCGACAATCGCCTCACCGAAAAGCCGGAGCTGAGCTCGAAGCTGCTGGGGAGGGCTGAAGGGTTCTACGCATCAGCTTCACAACAGGACACCATAACTGTGCTGGGGAGGAACCCGGTGTTCAACAAGGTGAGGGAAATGCCGGTGATCGGAGGAAGTGGACTTTTCAGGTTTGCAAGAGGTTATGTTGAAGCAAGAACTCACACATTCAATCTGAGCACCCGAAATGCCATCGTTGAGTACAATGCCTATGTGCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

175

Amino Acids

19.66

Weight (kDa)

9.39

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 116 4.7e-19 Dirigent-like protein
Dirigent PF03018 116 - 172 2.1e-19 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 1 cut(s) 120
AciI CCGC 2 cut(s) 118, 186
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 2 cut(s) 167, 503
AfiI CCNNNNNNNGG 2 cut(s) 73, 410
AgsI TTSAA 4 cut(s) 160, 385, 452, 472
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
AluI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
Alw21I GWGCWC 3 cut(s) 299, 482, 519
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 304
AspS9I GGNCC 2 cut(s) 76, 115
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 5 cut(s) 213, 226, 268, 403, 418
AvaII GGWCC 2 cut(s) 76, 115
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 135, 299
Bbv12I GWGCWC 3 cut(s) 299, 482, 519
BbvI GCAGC 1 cut(s) 291
BccI CCATC 2 cut(s) 26, 500
BciT130I CCWGG 1 cut(s) 80
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 1 cut(s) 377
BisI GCNGC 2 cut(s) 187, 305
BlpI GCTNAGC 2 cut(s) 149, 293
BlsI GCNGC 2 cut(s) 188, 306
Bme1390I CCNGG 2 cut(s) 80, 377
Bme18I GGWCC 2 cut(s) 76, 115
BmgT120I GGNCC 2 cut(s) 76, 115
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 2 cut(s) 80, 377
BmsI GCATC 1 cut(s) 341
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 2 cut(s) 149, 293
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 79, 111
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 410
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 2 cut(s) 79, 111
BseLI CCNNNNNNNGG 2 cut(s) 73, 410
BseMII CTCAG 3 cut(s) 163, 284, 467
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 291
BseYI CCCAGC 2 cut(s) 307, 364
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 3 cut(s) 299, 482, 519
BsiSI CCGG 3 cut(s) 287, 377, 404
BslI CCNNNNNNNGG 2 cut(s) 73, 410
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 4 cut(s) 135, 299, 482, 519
Bsp143I GATC 4 cut(s) 91, 121, 261, 408
Bsp1720I GCTNAGC 2 cut(s) 149, 293
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 2 cut(s) 118, 186
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 162, 285, 468
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 374
BspQI GCTCTTC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 403
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 2 cut(s) 79, 111
BssMI GATC 4 cut(s) 91, 121, 261, 408
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 33, 361
Bst6I CTCTTC 1 cut(s) 248
BstDEI CTNAG 3 cut(s) 149, 293, 476
BstDSI CCRYGG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 4 cut(s) 94, 124, 264, 411
BstMBI GATC 4 cut(s) 91, 121, 261, 408
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 78, 375
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 291
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 76, 115
Csp6I GTAC 2 cut(s) 166, 502
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 3 cut(s) 112, 173, 259
CviQI GTAC 2 cut(s) 166, 502
DdeI CTNAG 3 cut(s) 149, 293, 476
DpnI GATC 4 cut(s) 93, 123, 263, 410
DpnII GATC 4 cut(s) 91, 121, 261, 408
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 2 cut(s) 135, 299
Eco47I GGWCC 2 cut(s) 76, 115
Eco53kI GAGCTC 1 cut(s) 297
Eco57I CTGAAG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 2 cut(s) 135, 299
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 3 cut(s) 115, 176, 262
FaiI YATR 7 cut(s) 38, 113, 174, 260, 356, 447, 513
FatI CATG 3 cut(s) 111, 172, 258
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 187, 305
FriOI GRGCYC 2 cut(s) 135, 299
Fsp4HI GCNGC 2 cut(s) 187, 305
GluI GCNGC 2 cut(s) 187, 305
GsaI CCCAGC 2 cut(s) 311, 368
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 287, 377, 404
Hin1II CATG 3 cut(s) 115, 176, 262
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 1 cut(s) 249
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 3 cut(s) 287, 377, 404
HphI GGTGA 5 cut(s) 213, 226, 268, 403, 418
Hpy166II GTNNAC 3 cut(s) 229, 256, 422
Hpy188I TCNGA 4 cut(s) 209, 248, 413, 477
Hpy188III TCNNGA 4 cut(s) 95, 103, 136, 253
Hpy8I GTNNAC 3 cut(s) 229, 256, 422
HpyAV CCTTC 2 cut(s) 59, 314
HpyCH4III ACNGT 2 cut(s) 33, 361
HpyCH4V TGCA 1 cut(s) 437
HpyF3I CTNAG 3 cut(s) 149, 293, 476
Hsp92II CATG 3 cut(s) 115, 176, 262
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 4 cut(s) 91, 121, 261, 408
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 2 cut(s) 289, 522
Lsp1109I GCAGC 1 cut(s) 291
LweI GCATC 1 cut(s) 341
MalI GATC 4 cut(s) 93, 123, 263, 410
MbiI CCGCTC 1 cut(s) 186
MboI GATC 4 cut(s) 91, 121, 261, 408
MboII GAAGA 3 cut(s) 48, 98, 235
MhlI GDGCHC 4 cut(s) 135, 299, 482, 519
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 7 cut(s) 24, 284, 306, 363, 387, 407, 434
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 359
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 287, 377, 404
MspR9I CCNGG 2 cut(s) 80, 377
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 120
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 1 cut(s) 111
NdeII GATC 4 cut(s) 91, 121, 261, 408
NlaIII CATG 3 cut(s) 115, 176, 262
NlaIV GGNNCC 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 248
PfeI GAWTC 1 cut(s) 249
PkrI GCNGC 2 cut(s) 188, 306
Psp124BI GAGCTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 2 cut(s) 307, 364
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 2 cut(s) 76, 115
RsaI GTAC 2 cut(s) 167, 503
RsaNI GTAC 2 cut(s) 166, 502
RseI CAYNNNNRTG 1 cut(s) 359
SacI GAGCTC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 187, 305
Sau3AI GATC 4 cut(s) 91, 121, 261, 408
Sau96I GGNCC 2 cut(s) 76, 115
ScrFI CCNGG 2 cut(s) 80, 377
SduI GDGCHC 4 cut(s) 135, 299, 482, 519
SfaNI GCATC 1 cut(s) 341
SinI GGWCC 2 cut(s) 76, 115
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 1 cut(s) 69
SsiI CCGC 2 cut(s) 118, 186
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 78, 375
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 33, 361
TaqI TCGA 2 cut(s) 264, 299
TasI AATT 1 cut(s) 69
TatI WGTACW 2 cut(s) 165, 501
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseI GCWGC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 25
VpaK11BI GGWCC 2 cut(s) 76, 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.