RchiOBHm_Chr5g0081441

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000726
Physical Location & Seq
Reverse (-)
87395904 .. 87396494
591 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35569

Sequence Viewer

Length: 591 bp
ATGGCTAGAATCCTTCCTGTCTCAGCTTTCCAATTCATCATAATCTCCTCCCTCCTGTCTTCCTTTTCTATGATCTTAGTTTCTGGGGAAGATCATGAGTTTATGAAACCCATGGATCCGGAGCTTTTTGGTCTAAAGAAAGAAAAACTTACCCATTTTCGCGTGTATTGGCACGACATTGTTGATGGTCCCAGTCCCACTGCCGTGACAGTAGTGCAACCACTCTCCAACTCCTCCCAAACAAAGTTCGGGATGATACGAATGATCGACAACCAGCTAACCTTAGGCCCCGAATCGAGCTCGAAGCTGTTGGGAAGGGCTCAGGGGTTTTATGCATCTGCATCACAAGAAGACCTCAGTCTGTTGATGGCTCAGAACCTTGCATTTGTTCAGGGGAAGTATAACGGTAGCACCATAACCCTGATGGGAAGGAACTCGCTTACCAACAAGGTAAGGGAGCTGTCGGTGATCGGAGGAAGTGGACTTTTCAGGTTTGCTAGGGGTTATGCTCTACTAACAACTCAGTCGTTTAACGCCTCTAAAAGTGATGATGCTGTGGTGGAGTATAACATCTATGTCTTGCATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

196

Amino Acids

21.7

Weight (kDa)

7.96

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 193 2.8e-55 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 162
AccIII TCCGGA 1 cut(s) 118
AclWI GGATC 2 cut(s) 110, 123
AleI CACNNNNGTG 1 cut(s) 203
AluBI AGCT 6 cut(s) 26, 124, 277, 300, 307, 460
AluI AGCT 6 cut(s) 26, 124, 277, 300, 307, 460
Alw21I GWGCWC 1 cut(s) 302
Alw26I GTCTC 1 cut(s) 25
AlwI GGATC 2 cut(s) 110, 123
Aor13HI TCCGGA 1 cut(s) 118
AoxI GGCC 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 188, 287
AsuHPI GGTGA 1 cut(s) 478
AvaII GGWCC 1 cut(s) 188
AxyI CCTNAGG 1 cut(s) 283
BamHI GGATCC 1 cut(s) 115
BanII GRGCYC 2 cut(s) 302, 322
BbsI GAAGAC 2 cut(s) 51, 357
Bbv12I GWGCWC 1 cut(s) 302
BccI CCATC 3 cut(s) 179, 361, 418
BceAI ACGGC 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 2 cut(s) 6, 498
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 188, 287
BmiI GGNNCC 3 cut(s) 117, 190, 289
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 3 cut(s) 344, 350, 541
BmuI ACTGGG 1 cut(s) 186
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 51, 357
Bpu10I CCTNAGC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 111
BsaWI WCCGGW 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 192
Bse21I CCTNAGG 1 cut(s) 283
BseAI TCCGGA 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 1 cut(s) 258
BseMII CTCAG 5 cut(s) 36, 335, 370, 386, 536
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 2 cut(s) 37, 223
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 302
BsiSI CCGG 1 cut(s) 119
BslFI GGGAC 2 cut(s) 174, 180
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 2 cut(s) 174, 180
BsnI GGCC 1 cut(s) 288
Bsp1286I GDGCHC 2 cut(s) 302, 322
Bsp13I TCCGGA 1 cut(s) 118
Bsp143I GATC 5 cut(s) 72, 91, 115, 264, 468
Bsp19I CCATGG 1 cut(s) 111
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 5 cut(s) 35, 334, 369, 385, 535
BspEI TCCGGA 1 cut(s) 118
BspFNI CGCG 1 cut(s) 162
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 3 cut(s) 117, 190, 289
BspPI GGATC 2 cut(s) 110, 123
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 5 cut(s) 72, 91, 115, 264, 468
BssT1I CCWWGG 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 211, 407
BstDEI CTNAG 7 cut(s) 22, 76, 283, 321, 356, 372, 522
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 1 cut(s) 258
BstFNI CGCG 1 cut(s) 162
BstKTI GATC 5 cut(s) 75, 94, 118, 267, 471
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 5 cut(s) 72, 91, 115, 264, 468
BstPAI GACNNNNGTC 1 cut(s) 357
BstUI CGCG 1 cut(s) 162
BstV2I GAAGAC 2 cut(s) 51, 357
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
Bsu36I CCTNAGG 1 cut(s) 283
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 1 cut(s) 258
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
CciI TCATGA 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 188, 287
CviAII CATG 2 cut(s) 95, 112
DdeI CTNAG 7 cut(s) 22, 76, 283, 321, 356, 372, 522
DpnI GATC 5 cut(s) 74, 93, 117, 266, 470
DpnII GATC 5 cut(s) 72, 91, 115, 264, 468
Ecl136II GAGCTC 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 2 cut(s) 302, 322
Eco47I GGWCC 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 300
Eco81I CCTNAGG 1 cut(s) 283
EcoICRI GAGCTC 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 2 cut(s) 302, 322
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 98, 115
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FaqI GGGAC 2 cut(s) 174, 180
FatI CATG 2 cut(s) 94, 111
FokI GGATG 1 cut(s) 265
FriOI GRGCYC 2 cut(s) 302, 322
FspBI CTAG 2 cut(s) 6, 498
HaeIII GGCC 1 cut(s) 288
HapII CCGG 1 cut(s) 119
Hin1II CATG 2 cut(s) 98, 115
HinfI GANTC 2 cut(s) 9, 293
HpaII CCGG 1 cut(s) 119
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 1 cut(s) 482
Hpy188I TCNGA 2 cut(s) 375, 473
Hpy188III TCNNGA 3 cut(s) 95, 119, 250
Hpy8I GTNNAC 1 cut(s) 482
HpyAV CCTTC 3 cut(s) 23, 309, 423
HpyCH4III ACNGT 2 cut(s) 211, 407
HpyCH4V TGCA 5 cut(s) 217, 335, 341, 383, 583
HpyF3I CTNAG 7 cut(s) 22, 76, 283, 321, 356, 372, 522
Hsp92II CATG 2 cut(s) 98, 115
Kpn2I TCCGGA 1 cut(s) 118
Kzo9I GATC 5 cut(s) 72, 91, 115, 264, 468
LmnI GCTCC 2 cut(s) 121, 457
LweI GCATC 3 cut(s) 344, 350, 541
MaeI CTAG 2 cut(s) 6, 498
MaeIII GTNAC 1 cut(s) 205
MalI GATC 5 cut(s) 74, 93, 117, 266, 470
MboI GATC 5 cut(s) 72, 91, 115, 264, 468
MboII GAAGA 3 cut(s) 51, 101, 362
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 302, 322
MluCI AATT 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 6 cut(s) 58, 62, 244, 365, 467, 547
Mph1103I ATGCAT 1 cut(s) 337
MroI TCCGGA 1 cut(s) 118
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 1 cut(s) 119
MvnI CGCG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 111
NdeII GATC 5 cut(s) 72, 91, 115, 264, 468
NlaIII CATG 2 cut(s) 98, 115
NlaIV GGNNCC 3 cut(s) 117, 190, 289
NmuCI GTSAC 1 cut(s) 205
NsiI ATGCAT 1 cut(s) 337
OliI CACNNNNGTG 1 cut(s) 203
PagI TCATGA 1 cut(s) 94
PfeI GAWTC 2 cut(s) 9, 293
PshAI GACNNNNGTC 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 302
PspN4I GGNNCC 3 cut(s) 117, 190, 289
PspPI GGNCC 2 cut(s) 188, 287
PsuI RGATCY 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 203
SacI GAGCTC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 531
Sau3AI GATC 5 cut(s) 72, 91, 115, 264, 468
Sau96I GGNCC 2 cut(s) 188, 287
SduI GDGCHC 2 cut(s) 302, 322
SfaNI GCATC 3 cut(s) 344, 350, 541
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 1 cut(s) 32
SspMI CTAG 2 cut(s) 6, 498
SstI GAGCTC 1 cut(s) 302
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 211, 407
TaqI TCGA 3 cut(s) 267, 296, 302
TasI AATT 1 cut(s) 32
TfiI GAWTC 2 cut(s) 9, 293
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 25, 119
TspRI CASTG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XspI CTAG 2 cut(s) 6, 498
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.