Rmu_co8330035.1_g000001

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8330035.1
OG0000726
Physical Location & Seq
Reverse (-)
2 .. 423
422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8330035.1_g000001.1.cds

Sequence Viewer

Length: 422 bp
atggccagaatacctcccatcgttgctacacagttcatagttctctcccttctttcttcctttgccctaattctggtccagggagaagatgatcacgatttcgtgagagccatggaccgcgatctcttggggctcaagaaagaaaagctcagccacttcaagttgtactggcatgacattgtgagcggcaaaaacccaagctcaatcggagtcgtgaaatcacccgttaactcatcaacgctcttcggattcgtgagtatgatcgacgatcccctcaccgaaaagccggagctgagctcgaagctactggggagggctcaagggttctacgcatcagcttctcaacaggaggtcgggctgttgatggctatgaactttcattttattcagggcaagtacaacgggagcaccataaccgtgct
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

141

Amino Acids

15.56

Weight (kDa)

6.9

Isoelectric Point (pI)

24.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 1 cut(s) 120
AciI CCGC 2 cut(s) 118, 186
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 2 cut(s) 167, 398
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 1 cut(s) 160
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
AluI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
Alw21I GWGCWC 2 cut(s) 299, 410
AlwI GGATC 1 cut(s) 263
AoxI GGCC 1 cut(s) 3
AspS9I GGNCC 2 cut(s) 76, 115
AsuHPI GGTGA 2 cut(s) 213, 268
AvaII GGWCC 2 cut(s) 76, 115
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 3 cut(s) 135, 299, 319
Bbv12I GWGCWC 2 cut(s) 299, 410
BccI CCATC 2 cut(s) 26, 358
BciT130I CCWGG 1 cut(s) 80
BclI TGATCA 1 cut(s) 91
BisI GCNGC 1 cut(s) 187
BlpI GCTNAGC 2 cut(s) 149, 293
BlsI GCNGC 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 80
Bme18I GGWCC 2 cut(s) 76, 115
BmgT120I GGNCC 2 cut(s) 76, 115
BmrFI CCNGG 1 cut(s) 80
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 1 cut(s) 341
BmuI ACTGGG 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 2 cut(s) 149, 293
BpuEI CTTGAG 2 cut(s) 119, 303
BsaJI CCNNGG 2 cut(s) 79, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 173, 312
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 2 cut(s) 79, 111
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 2 cut(s) 163, 284
BseNI ACTGG 2 cut(s) 173, 312
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 299, 410
BsiSI CCGG 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 73
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 4 cut(s) 135, 299, 319, 410
Bsp143I GATC 4 cut(s) 91, 121, 261, 268
Bsp1720I GCTNAGC 2 cut(s) 149, 293
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 2 cut(s) 118, 186
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 162, 285
BspFNI CGCG 1 cut(s) 120
BspPI GGATC 1 cut(s) 263
BspQI GCTCTTC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 186
BsrI ACTGG 2 cut(s) 173, 312
BssECI CCNNGG 2 cut(s) 79, 111
BssMI GATC 4 cut(s) 91, 121, 261, 268
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 33, 418
Bst6I CTCTTC 1 cut(s) 248
BstDEI CTNAG 2 cut(s) 149, 293
BstDSI CCRYGG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 4 cut(s) 94, 124, 264, 271
BstMBI GATC 4 cut(s) 91, 121, 261, 268
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 78
BstUI CGCG 1 cut(s) 120
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 111
Cfr13I GGNCC 2 cut(s) 76, 115
Csp6I GTAC 2 cut(s) 166, 397
CviAII CATG 2 cut(s) 112, 173
CviQI GTAC 2 cut(s) 166, 397
DdeI CTNAG 2 cut(s) 149, 293
DpnI GATC 4 cut(s) 93, 123, 263, 270
DpnII GATC 4 cut(s) 91, 121, 261, 268
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 3 cut(s) 135, 299, 319
Eco47I GGWCC 2 cut(s) 76, 115
Eco53kI GAGCTC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 3 cut(s) 135, 299, 319
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 115, 176
FaiI YATR 6 cut(s) 38, 113, 174, 260, 371, 413
FatI CATG 2 cut(s) 111, 172
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 1 cut(s) 187
FriOI GRGCYC 3 cut(s) 135, 299, 319
Fsp4HI GCNGC 1 cut(s) 187
GluI GCNGC 1 cut(s) 187
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 287
Hin1II CATG 2 cut(s) 115, 176
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 2 cut(s) 210, 249
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 1 cut(s) 287
HphI GGTGA 2 cut(s) 213, 268
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 209, 248
Hpy188III TCNNGA 5 cut(s) 95, 103, 136, 214, 253
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 33, 418
HpyF3I CTNAG 2 cut(s) 149, 293
Hsp92II CATG 2 cut(s) 115, 176
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 4 cut(s) 91, 121, 261, 268
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 2 cut(s) 289, 405
LpnPI CCDG 9 cut(s) 19, 59, 65, 92, 154, 293, 300, 332, 374
LweI GCATC 1 cut(s) 341
MalI GATC 4 cut(s) 93, 123, 263, 270
MbiI CCGCTC 1 cut(s) 186
MboI GATC 4 cut(s) 91, 121, 261, 268
MboII GAAGA 3 cut(s) 48, 98, 235
MhlI GDGCHC 4 cut(s) 135, 299, 319, 410
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 219
MnlI CCTC 4 cut(s) 24, 284, 306, 343
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 120
NcoI CCATGG 1 cut(s) 111
NdeII GATC 4 cut(s) 91, 121, 261, 268
NlaIII CATG 2 cut(s) 115, 176
PciSI GCTCTTC 1 cut(s) 248
PfeI GAWTC 1 cut(s) 249
PkrI GCNGC 1 cut(s) 188
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspPI GGNCC 2 cut(s) 76, 115
RsaI GTAC 2 cut(s) 167, 398
RsaNI GTAC 2 cut(s) 166, 397
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 4 cut(s) 91, 121, 261, 268
Sau96I GGNCC 2 cut(s) 76, 115
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 4 cut(s) 135, 299, 319, 410
SetI ASST 8 cut(s) 16, 150, 203, 294, 299, 306, 340, 354
SfaNI GCATC 1 cut(s) 341
SinI GGWCC 2 cut(s) 76, 115
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 134, 318
SmoI CTYRAG 2 cut(s) 134, 318
Sse9I AATT 1 cut(s) 69
SsiI CCGC 2 cut(s) 118, 186
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 33, 418
TaqI TCGA 2 cut(s) 264, 299
TasI AATT 1 cut(s) 69
TatI WGTACW 2 cut(s) 165, 396
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TspDTI ATGAA 3 cut(s) 25, 368, 386
VpaK11BI GGWCC 2 cut(s) 76, 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.