MD11G1012400.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000726
Physical Location & Seq
Forward (+)
912154 .. 912735
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1012400.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCTACAACACCTCAAATCTTGGCTACTCAGTTCATAATTCTCTCACTTCTATCTTCATTTGCCCTAATTCTTGTCCACGGAGAAGATCAAGATTTTGTTAGAGCCTTGGACCGCAAGCTTCTGGGTCTCAAGAAAGAGAAGTTCAGCCACTTTAAGATGTACTGGCATGATATTTACAGCGGTAAAAATCCAAGTGCAGTAGGAGTGGTGAATCCACCCATCAATTCATCAACCCTATTTGGTTCTGTTAGCATGATTGATGATCCTCTTACTGAAGGCCCTGAGTCGAGCTCAAAATTGCTGGGAAAGGCTCAAGGGTTCTATGCATCTGCCTCACAGAAAGAAGTTGGCCTGCTGATGGCCATGAATTTTCACTTTAACCAAGGGAAGTACAATGGCAGCACCATAACGATTCTAGGAAGGAACCAGGTGTTCGACAAGGTTAGGGAGATGCCTGTCATTGGAGGCAGTGGGCTTTTCAGGTTTGCAAGAGGTTATGTTCAAGCAAGTACTCATACATTCAATCCAACCACAGGAGATGCCGTTGTTGAGTATAATATCTATGTGCTGCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

194

Amino Acids

21.32

Weight (kDa)

8.76

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 50 - 190 4.1e-59 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 115, 183
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 3 cut(s) 164, 395, 514
AfiI CCNNNNNNNGG 3 cut(s) 361, 464, 536
AgsI TTSAA 2 cut(s) 506, 526
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 2 cut(s) 121, 294
AluI AGCT 2 cut(s) 121, 294
Alw21I GWGCWC 1 cut(s) 296
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 1 cut(s) 260
AoxI GGCC 3 cut(s) 280, 352, 363
ApeKI GCWGC 2 cut(s) 402, 571
ApoI RAATTY 1 cut(s) 370
AspS9I GGNCC 2 cut(s) 112, 281
AsuHPI GGTGA 1 cut(s) 223
AvaII GGWCC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 365
BanII GRGCYC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 414, 558
BccI CCATC 2 cut(s) 230, 355
BceAI ACGGC 1 cut(s) 530
BciT130I CCWGG 1 cut(s) 431
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 1 cut(s) 419
BisI GCNGC 2 cut(s) 403, 572
BlsI GCNGC 2 cut(s) 404, 573
BmcAI AGTACT 1 cut(s) 514
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 112, 281
BmiI GGNNCC 1 cut(s) 428
BmrFI CCNGG 1 cut(s) 431
BmsI GCATC 3 cut(s) 338, 444, 532
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpuEI CTTGAG 2 cut(s) 116, 300
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 79, 108, 385
BsaXI ACNNNNNCTCC 2 cut(s) 531, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 464, 536
Bse1I ACTGG 1 cut(s) 170
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 3 cut(s) 79, 108, 385
BseLI CCNNNNNNNGG 3 cut(s) 361, 464, 536
BseMII CTCAG 2 cut(s) 44, 276
BseNI ACTGG 1 cut(s) 170
BseXI GCAGC 2 cut(s) 414, 558
BseYI CCCAGC 1 cut(s) 304
BsgI GTGCAG 1 cut(s) 219
BshFI GGCC 3 cut(s) 282, 354, 365
BsiHKAI GWGCWC 1 cut(s) 296
BslI CCNNNNNNNGG 3 cut(s) 361, 464, 536
BsmAI GTCTC 1 cut(s) 134
BsnI GGCC 3 cut(s) 282, 354, 365
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 2 cut(s) 88, 265
BspACI CCGC 2 cut(s) 115, 183
BspANI GGCC 3 cut(s) 282, 354, 365
BspCNI CTCAG 2 cut(s) 43, 277
BspLI GGNNCC 1 cut(s) 428
BspPI GGATC 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 134
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 3 cut(s) 79, 108, 385
BssMI GATC 2 cut(s) 88, 265
BssT1I CCWWGG 2 cut(s) 108, 385
Bst2UI CCWGG 1 cut(s) 431
BstC8I GCNNGC 2 cut(s) 119, 356
BstDEI CTNAG 2 cut(s) 30, 285
BstDSI CCRYGG 1 cut(s) 79
BstKTI GATC 2 cut(s) 91, 268
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 88, 265
BstNI CCWGG 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 429
BstV1I GCAGC 2 cut(s) 414, 558
BsuRI GGCC 3 cut(s) 282, 354, 365
BtgI CCRYGG 1 cut(s) 79
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 1 cut(s) 478
Cac8I GCNNGC 2 cut(s) 119, 356
Cfr13I GGNCC 2 cut(s) 112, 281
CsiI ACCWGGT 1 cut(s) 429
Csp6I GTAC 3 cut(s) 163, 394, 513
CviAII CATG 3 cut(s) 170, 256, 367
CviQI GTAC 3 cut(s) 163, 394, 513
DdeI CTNAG 2 cut(s) 30, 285
DpnI GATC 2 cut(s) 90, 267
DpnII GATC 2 cut(s) 88, 265
EaeI YGGCCR 1 cut(s) 363
Ecl136II GAGCTC 1 cut(s) 294
Eco130I CCWWGG 2 cut(s) 108, 385
Eco24I GRGCYC 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 108, 385
EcoT22I ATGCAT 1 cut(s) 331
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 2 cut(s) 108, 385
FaeI CATG 3 cut(s) 173, 259, 370
FatI CATG 3 cut(s) 169, 255, 366
Fnu4HI GCNGC 2 cut(s) 403, 572
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 403, 572
FspBI CTAG 1 cut(s) 419
GluI GCNGC 2 cut(s) 403, 572
GsaI CCCAGC 1 cut(s) 308
HaeIII GGCC 3 cut(s) 282, 354, 365
Hin1II CATG 3 cut(s) 173, 259, 370
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 3 cut(s) 214, 287, 415
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 79
Hpy188III TCNNGA 2 cut(s) 92, 133
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 272, 417
HpyCH4V TGCA 4 cut(s) 200, 329, 491, 574
HpyF3I CTNAG 2 cut(s) 30, 285
Hsp92II CATG 3 cut(s) 173, 259, 370
Kzo9I GATC 2 cut(s) 88, 265
Lsp1109I GCAGC 2 cut(s) 414, 558
LweI GCATC 3 cut(s) 338, 444, 532
MabI ACCWGGT 1 cut(s) 429
MaeI CTAG 1 cut(s) 419
MalI GATC 2 cut(s) 90, 267
MboI GATC 2 cut(s) 88, 265
MboII GAAGA 2 cut(s) 48, 98
MhlI GDGCHC 1 cut(s) 296
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 5 cut(s) 39, 69, 226, 299, 370
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 554
MnlI CCTC 5 cut(s) 24, 279, 346, 461, 488
Mox20I TGGCCA 1 cut(s) 365
Mph1103I ATGCAT 1 cut(s) 331
MscI TGGCCA 1 cut(s) 365
MseI TTAA 2 cut(s) 156, 381
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 183
MspR9I CCNGG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 431
NdeII GATC 2 cut(s) 88, 265
NlaIII CATG 3 cut(s) 173, 259, 370
NlaIV GGNNCC 1 cut(s) 428
NsiI ATGCAT 1 cut(s) 331
PfeI GAWTC 2 cut(s) 214, 415
PkrI GCNGC 2 cut(s) 404, 573
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Psp124BI GAGCTC 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 304
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 428
PspPI GGNCC 2 cut(s) 112, 281
RsaI GTAC 3 cut(s) 164, 395, 514
RsaNI GTAC 3 cut(s) 163, 394, 513
SacI GAGCTC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 156, 381
SatI GCNGC 2 cut(s) 403, 572
Sau3AI GATC 2 cut(s) 88, 265
Sau96I GGNCC 2 cut(s) 112, 281
ScaI AGTACT 1 cut(s) 514
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 1 cut(s) 296
SetI ASST 7 cut(s) 16, 123, 296, 435, 447, 488, 499
SexAI ACCWGGT 1 cut(s) 429
SfaNI GCATC 3 cut(s) 338, 444, 532
SinI GGWCC 1 cut(s) 112
SmlI CTYRAG 2 cut(s) 131, 315
SmoI CTYRAG 2 cut(s) 131, 315
Sse9I AATT 5 cut(s) 39, 69, 226, 299, 370
SsiI CCGC 2 cut(s) 115, 183
SspMI CTAG 1 cut(s) 419
SstI GAGCTC 1 cut(s) 296
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 2 cut(s) 108, 385
TaqI TCGA 2 cut(s) 290, 438
TasI AATT 5 cut(s) 39, 69, 226, 299, 370
TatI WGTACW 3 cut(s) 162, 393, 512
TfiI GAWTC 2 cut(s) 214, 415
Tru1I TTAA 2 cut(s) 156, 381
Tru9I TTAA 2 cut(s) 156, 381
TscAI CASTG 1 cut(s) 478
TseI GCWGC 2 cut(s) 402, 571
TspDTI ATGAA 4 cut(s) 25, 48, 219, 383
TspGWI ACGGA 1 cut(s) 96
TspRI CASTG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 370
XspI CTAG 1 cut(s) 419
ZrmI AGTACT 1 cut(s) 514
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.