Rmu_co8102938.1_g000001

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8102938.1
OG0000726
Physical Location & Seq
Reverse (-)
58 .. 579
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8102938.1_g000001.1.cds

Sequence Viewer

Length: 522 bp
atgatcttagtttctggggaagatcatgagtttatgaaacccatggatcccgagctttttggtctaaagaaagaaaaacttacccattttcgcgtgtattggcacgacattgttgatggtcctagtcccactgccgtgacagtagtgcaaccactctccaactcctcccaaacaaagttcgggatgatacgaatgatcgacaaccagctaaccttaggccccgaatcgagctcgaagctgttgggaagggctcaagggttttatgcatctgcatcacaagaagacctcagtctgttgatggctcagaactttgcatttgttcaggggaagcataacggtagcaccataaccctgatgggaaggaactcgcttaccaacaaggtaagggagctgtcggtgatcggaggaagtggacttttcaggtttgctaggggttatgctctactaacaactcaatcgtttaacgcctctaaaagtggtgatgctgtggtggagtataacatctatgtcttgcattactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

173

Amino Acids

19.14

Weight (kDa)

7.99

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 93
AclWI GGATC 2 cut(s) 41, 54
AleI CACNNNNGTG 1 cut(s) 134
AluBI AGCT 5 cut(s) 55, 208, 231, 238, 391
AluI AGCT 5 cut(s) 55, 208, 231, 238, 391
Alw21I GWGCWC 1 cut(s) 233
AlwI GGATC 2 cut(s) 41, 54
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 119, 218
AsuHPI GGTGA 2 cut(s) 409, 491
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 119
AxyI CCTNAGG 1 cut(s) 214
BamHI GGATCC 1 cut(s) 46
BanII GRGCYC 2 cut(s) 233, 253
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 233
BccI CCATC 3 cut(s) 110, 292, 349
BceAI ACGGC 1 cut(s) 119
BfaI CTAG 2 cut(s) 123, 429
Bme18I GGWCC 1 cut(s) 119
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 119, 218
BmiI GGNNCC 2 cut(s) 48, 220
BmsI GCATC 3 cut(s) 275, 281, 472
BoxI GACNNNNGTC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 42
Bse21I CCTNAGG 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 1 cut(s) 189
BseMII CTCAG 2 cut(s) 301, 317
BseRI GAGGAG 1 cut(s) 154
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 111
BsnI GGCC 1 cut(s) 219
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 233, 253
Bsp143I GATC 5 cut(s) 3, 22, 46, 195, 399
Bsp19I CCATGG 1 cut(s) 42
BspANI GGCC 1 cut(s) 219
BspCNI CTCAG 2 cut(s) 300, 316
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 2 cut(s) 48, 220
BspPI GGATC 2 cut(s) 41, 54
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 5 cut(s) 3, 22, 46, 195, 399
BssT1I CCWWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 142, 338
BstDEI CTNAG 4 cut(s) 7, 214, 287, 303
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 1 cut(s) 93
BstKTI GATC 5 cut(s) 6, 25, 49, 198, 402
BstMBI GATC 5 cut(s) 3, 22, 46, 195, 399
BstPAI GACNNNNGTC 1 cut(s) 288
BstUI CGCG 1 cut(s) 93
BstV2I GAAGAC 1 cut(s) 288
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 214
BsuRI GGCC 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 189
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 129
CciI TCATGA 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 119, 218
CviAII CATG 2 cut(s) 26, 43
CviJI RGCY 8 cut(s) 55, 208, 219, 231, 238, 251, 302, 391
CviKI_1 RGCY 8 cut(s) 55, 208, 219, 231, 238, 251, 302, 391
DdeI CTNAG 4 cut(s) 7, 214, 287, 303
DpnI GATC 5 cut(s) 5, 24, 48, 197, 401
DpnII GATC 5 cut(s) 3, 22, 46, 195, 399
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 42
Eco24I GRGCYC 2 cut(s) 233, 253
Eco47I GGWCC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 231
Eco81I CCTNAGG 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 218
EcoT14I CCWWGG 1 cut(s) 42
EcoT22I ATGCAT 1 cut(s) 268
EcoT38I GRGCYC 2 cut(s) 233, 253
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 2 cut(s) 29, 46
FaiI YATR 9 cut(s) 27, 35, 44, 264, 333, 347, 438, 498, 507
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FaqI GGGAC 1 cut(s) 111
FatI CATG 2 cut(s) 25, 42
FokI GGATG 1 cut(s) 196
FriOI GRGCYC 2 cut(s) 233, 253
FspBI CTAG 2 cut(s) 123, 429
HaeIII GGCC 1 cut(s) 219
Hin1II CATG 2 cut(s) 29, 46
HinfI GANTC 1 cut(s) 224
HphI GGTGA 2 cut(s) 409, 491
Hpy166II GTNNAC 1 cut(s) 413
Hpy188I TCNGA 2 cut(s) 306, 404
Hpy188III TCNNGA 3 cut(s) 26, 50, 181
Hpy8I GTNNAC 1 cut(s) 413
HpyAV CCTTC 2 cut(s) 240, 354
HpyCH4III ACNGT 2 cut(s) 142, 338
HpyCH4V TGCA 5 cut(s) 148, 266, 272, 314, 514
HpyF3I CTNAG 4 cut(s) 7, 214, 287, 303
Hsp92II CATG 2 cut(s) 29, 46
Kzo9I GATC 5 cut(s) 3, 22, 46, 195, 399
LmnI GCTCC 1 cut(s) 388
LpnPI CCDG 4 cut(s) 218, 308, 365, 406
LweI GCATC 3 cut(s) 275, 281, 472
MaeI CTAG 2 cut(s) 123, 429
MaeIII GTNAC 1 cut(s) 136
MalI GATC 5 cut(s) 5, 24, 48, 197, 401
MboI GATC 5 cut(s) 3, 22, 46, 195, 399
MboII GAAGA 2 cut(s) 32, 293
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 2 cut(s) 233, 253
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 4 cut(s) 175, 296, 398, 478
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 1 cut(s) 134
MvnI CGCG 1 cut(s) 93
NcoI CCATGG 1 cut(s) 42
NdeII GATC 5 cut(s) 3, 22, 46, 195, 399
NlaIII CATG 2 cut(s) 29, 46
NlaIV GGNNCC 2 cut(s) 48, 220
NmuCI GTSAC 1 cut(s) 136
NsiI ATGCAT 1 cut(s) 268
OliI CACNNNNGTG 1 cut(s) 134
PagI TCATGA 1 cut(s) 25
PfeI GAWTC 1 cut(s) 224
PshAI GACNNNNGTC 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 233
PspN4I GGNNCC 2 cut(s) 48, 220
PspPI GGNCC 2 cut(s) 119, 218
PsuI RGATCY 1 cut(s) 46
RseI CAYNNNNRTG 1 cut(s) 134
SacI GAGCTC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 462
Sau3AI GATC 5 cut(s) 3, 22, 46, 195, 399
Sau96I GGNCC 2 cut(s) 119, 218
SduI GDGCHC 2 cut(s) 233, 253
SetI ASST 9 cut(s) 57, 210, 215, 233, 240, 288, 384, 393, 425
SfaNI GCATC 3 cut(s) 275, 281, 472
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 252
SmoI CTYRAG 1 cut(s) 252
SspMI CTAG 2 cut(s) 123, 429
SstI GAGCTC 1 cut(s) 233
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 142, 338
TaqI TCGA 3 cut(s) 198, 227, 233
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 50
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XspI CTAG 2 cut(s) 123, 429
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.