RLG00000036941

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000726
Physical Location & Seq
Reverse (-)
86726750 .. 86727334
585 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036941

Sequence Viewer

Length: 585 bp
ATGGCCAGATTACTTCCCATCTGTGCTACACAGTTCATAGTTCTCTCCCTTCTTTCTTCCTTTGCCCTAATTCTGGTCCAGGGAGAAGATGATCACGATTTCGTGAAAGCCATGGACCGAGATCTCTTGGGGCTCAAGAAAGAAAAGCTCAGCCACTTCAAATTGTACTGGCATGACATTCAGAGCGGCAAAAACCCAAGCTCAATCGGAGTGGTGAAATCACCCGTCAACTCATCGACGCTCTTCGGATTGGTGAACATGATCGACAATCCCCTCACCGAAAAGCCGGAGCTGAGCTCGAAGCTACTGGGGAGGGCTCAAGGGTTCTACGCATCAGCTTCACAACAGGAGATCGGGCTGTTGATGGCTATGAACTTTCATTTTATTCAGGGCAAGTACAACGGGAGCACCATAACCGTGCTGGGGAGGAACCCCGTGTTCAACAAGGTGAGGGAGATGCCGGTGATCGGAGGAAGTGGGCTTTTCAGGTTTGCAAGAGGTTATGTTGAAGCAAGAACTCATACATTCACTGCGAGCACCGGAGATGCCATCGTTGAGTACAAGGCCTATGTGCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

195

Amino Acids

21.52

Weight (kDa)

9.16

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 191 1.2e-55 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 186
AciI CCGC 1 cut(s) 186
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 3 cut(s) 167, 398, 560
AfiI CCNNNNNNNGG 3 cut(s) 73, 423, 467
AgsI TTSAA 3 cut(s) 160, 442, 509
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
AluI AGCT 6 cut(s) 148, 201, 292, 297, 304, 338
Alw21I GWGCWC 4 cut(s) 299, 410, 539, 576
AoxI GGCC 2 cut(s) 3, 564
AspS9I GGNCC 2 cut(s) 76, 115
AsuHPI GGTGA 6 cut(s) 213, 226, 265, 268, 460, 475
AvaII GGWCC 2 cut(s) 76, 115
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 3 cut(s) 135, 299, 319
Bbv12I GWGCWC 4 cut(s) 299, 410, 539, 576
BccI CCATC 3 cut(s) 26, 358, 557
BciT130I CCWGG 1 cut(s) 80
BclI TGATCA 1 cut(s) 91
BglII AGATCT 1 cut(s) 121
BisI GCNGC 1 cut(s) 187
BlpI GCTNAGC 2 cut(s) 149, 293
BlsI GCNGC 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 80
Bme18I GGWCC 2 cut(s) 76, 115
BmgT120I GGNCC 2 cut(s) 76, 115
BmiI GGNNCC 1 cut(s) 431
BmrFI CCNGG 1 cut(s) 80
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 3 cut(s) 341, 447, 535
BmuI ACTGGG 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 2 cut(s) 149, 293
BpuEI CTTGAG 2 cut(s) 119, 303
BsaJI CCNNGG 2 cut(s) 79, 111
BsaWI WCCGGW 1 cut(s) 539
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 423, 467
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 173, 312
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 2 cut(s) 79, 111
BseLI CCNNNNNNNGG 3 cut(s) 73, 423, 467
BseMII CTCAG 2 cut(s) 163, 284
BseNI ACTGG 2 cut(s) 173, 312
BseYI CCCAGC 1 cut(s) 421
BshFI GGCC 2 cut(s) 5, 566
BsiHKAI GWGCWC 4 cut(s) 299, 410, 539, 576
BsiSI CCGG 3 cut(s) 287, 461, 540
BslI CCNNNNNNNGG 3 cut(s) 73, 423, 467
BsnI GGCC 2 cut(s) 5, 566
Bsp1286I GDGCHC 6 cut(s) 135, 299, 319, 410, 539, 576
Bsp143I GATC 5 cut(s) 91, 121, 261, 351, 465
Bsp1720I GCTNAGC 2 cut(s) 149, 293
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 1 cut(s) 186
BspANI GGCC 2 cut(s) 5, 566
BspCNI CTCAG 2 cut(s) 162, 285
BspLI GGNNCC 1 cut(s) 431
BspQI GCTCTTC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 2 cut(s) 173, 312
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 79, 111
BssMI GATC 5 cut(s) 91, 121, 261, 351, 465
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 2 cut(s) 33, 418
Bst6I CTCTTC 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 2 cut(s) 149, 293
BstDSI CCRYGG 1 cut(s) 111
BstKTI GATC 5 cut(s) 94, 124, 264, 354, 468
BstMBI GATC 5 cut(s) 91, 121, 261, 351, 465
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 78
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 2 cut(s) 5, 566
BtgI CCRYGG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 535
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 76, 115
CseI GACGC 1 cut(s) 247
Csp6I GTAC 3 cut(s) 166, 397, 559
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 3 cut(s) 112, 173, 259
CviQI GTAC 3 cut(s) 166, 397, 559
DdeI CTNAG 2 cut(s) 149, 293
DpnI GATC 5 cut(s) 93, 123, 263, 353, 467
DpnII GATC 5 cut(s) 91, 121, 261, 351, 465
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 111
Eco147I AGGCCT 1 cut(s) 566
Eco24I GRGCYC 3 cut(s) 135, 299, 319
Eco47I GGWCC 2 cut(s) 76, 115
Eco53kI GAGCTC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 3 cut(s) 135, 299, 319
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 3 cut(s) 115, 176, 262
FaiI YATR 9 cut(s) 38, 113, 174, 260, 371, 413, 504, 522, 570
FatI CATG 3 cut(s) 111, 172, 258
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 1 cut(s) 187
FriOI GRGCYC 3 cut(s) 135, 299, 319
Fsp4HI GCNGC 1 cut(s) 187
GluI GCNGC 1 cut(s) 187
GsaI CCCAGC 1 cut(s) 425
HaeIII GGCC 2 cut(s) 5, 566
HapII CCGG 3 cut(s) 287, 461, 540
HgaI GACGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 115, 176, 262
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HpaII CCGG 3 cut(s) 287, 461, 540
HphI GGTGA 6 cut(s) 213, 226, 265, 268, 460, 475
Hpy166II GTNNAC 2 cut(s) 229, 256
Hpy188I TCNGA 4 cut(s) 183, 209, 248, 470
Hpy188III TCNNGA 3 cut(s) 95, 103, 136
Hpy8I GTNNAC 2 cut(s) 229, 256
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 33, 418
HpyCH4V TGCA 1 cut(s) 494
HpyF3I CTNAG 2 cut(s) 149, 293
Hsp92II CATG 3 cut(s) 115, 176, 262
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 5 cut(s) 91, 121, 261, 351, 465
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 3 cut(s) 289, 405, 579
LweI GCATC 3 cut(s) 341, 447, 535
MalI GATC 5 cut(s) 93, 123, 263, 353, 467
MbiI CCGCTC 1 cut(s) 186
MboI GATC 5 cut(s) 91, 121, 261, 351, 465
MboII GAAGA 3 cut(s) 48, 98, 235
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 6 cut(s) 135, 299, 319, 410, 539, 576
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 69, 161
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 284, 306, 420, 444, 464, 491
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 287, 461, 540
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
NcoI CCATGG 1 cut(s) 111
NdeII GATC 5 cut(s) 91, 121, 261, 351, 465
NlaIII CATG 3 cut(s) 115, 176, 262
NlaIV GGNNCC 1 cut(s) 431
PceI AGGCCT 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 248
PkrI GCNGC 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 421
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 431
PspPI GGNCC 2 cut(s) 76, 115
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 3 cut(s) 167, 398, 560
RsaNI GTAC 3 cut(s) 166, 397, 559
RseI CAYNNNNRTG 1 cut(s) 416
SacI GAGCTC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 248
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 5 cut(s) 91, 121, 261, 351, 465
Sau96I GGNCC 2 cut(s) 76, 115
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 6 cut(s) 135, 299, 319, 410, 539, 576
SetI ASST 9 cut(s) 150, 203, 294, 299, 306, 340, 450, 491, 502
SfaNI GCATC 3 cut(s) 341, 447, 535
SinI GGWCC 2 cut(s) 76, 115
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 134, 318
SmoI CTYRAG 2 cut(s) 134, 318
Sse9I AATT 2 cut(s) 69, 161
SseBI AGGCCT 1 cut(s) 566
SsiI CCGC 1 cut(s) 186
SstI GAGCTC 1 cut(s) 299
StuI AGGCCT 1 cut(s) 566
StyD4I CCNGG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 33, 418
TaqI TCGA 3 cut(s) 236, 264, 299
TaqII GACCGA 1 cut(s) 132
TasI AATT 2 cut(s) 69, 161
TatI WGTACW 3 cut(s) 165, 396, 558
TauI GCSGC 1 cut(s) 189
TscAI CASTG 1 cut(s) 535
TspDTI ATGAA 3 cut(s) 25, 368, 386
TspRI CASTG 1 cut(s) 535
VpaK11BI GGWCC 2 cut(s) 76, 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.