Prupe.6G010300_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000726
Physical Location & Seq
Forward (+)
786169 .. 787159
991 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G010300.1

Sequence Viewer

Length: 585 bp
ATGGCCAAAACACCTTCTTCCATCTTGGCTACCCAGTTCATAATTCTCTCTCTTCTTTCTTCATTTGCCATAATTTTGGTCCAAGGAGAAGATCAAGGTTTTGTGAGATCCCTGGACCCCAAGCTTTTGGGGCTCAAGAAAGAGAAGCTGAGCCACTTTAGGTTTTACTGGCATGACATTTACAGTGGTAAAAATCCCAGTGCAGTAGGAGTTGTGAGAGCACCCACCAATTCATCAACCCTATTTGGTTCAGTTAGCATGATTGATGATCCTCTGACTCAAGGCCCTGAGTTGAGCTCAAAATTGTGGGGAAGGGCTCAAGGGTTCTATGCATCTGCCTCACAGCAAGAAGTAGGCCTTCTGATGGCTATGAATTTTCATTTCATTGAAGGCAAATACAATGGAAGCACCGTATCTGTGCTGGGAAGGAACCAAGTTTTCAACAAGGTGAGGGAGATGCCTATCATTGGAGGCAGTGGGCTTTTCAGGTTTGCAAGGGGTTATGTTGAAGCAAGAACTCACTCGTTCACTCCAAATACTGGAGATGCCATAGTTGAGTATAATGTCTATGTGCTGCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

195

Amino Acids

21.45

Weight (kDa)

9.33

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 539
AclWI GGATC 2 cut(s) 102, 263
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 373
AfiI CCNNNNNNNGG 3 cut(s) 364, 467, 539
AgsI TTSAA 3 cut(s) 389, 442, 509
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 3 cut(s) 124, 148, 297
AluI AGCT 3 cut(s) 124, 148, 297
Alw21I GWGCWC 2 cut(s) 223, 299
AlwI GGATC 2 cut(s) 102, 263
AoxI GGCC 3 cut(s) 3, 283, 355
ApeKI GCWGC 1 cut(s) 574
ApoI RAATTY 1 cut(s) 373
AspS9I GGNCC 3 cut(s) 79, 115, 284
AsuHPI GGTGA 1 cut(s) 460
AvaII GGWCC 2 cut(s) 79, 115
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 3 cut(s) 135, 299, 319
Bbv12I GWGCWC 2 cut(s) 223, 299
BbvI GCAGC 1 cut(s) 561
BccI CCATC 2 cut(s) 29, 358
BciT130I CCWGG 1 cut(s) 113
BisI GCNGC 1 cut(s) 575
BlpI GCTNAGC 1 cut(s) 149
BlsI GCNGC 1 cut(s) 576
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 2 cut(s) 79, 115
BmgT120I GGNCC 3 cut(s) 79, 115, 284
BmiI GGNNCC 2 cut(s) 117, 431
BmrFI CCNGG 1 cut(s) 113
BmrI ACTGGG 2 cut(s) 28, 192
BmsI GCATC 3 cut(s) 341, 447, 535
BmuI ACTGGG 2 cut(s) 28, 192
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpmI CTGGAG 1 cut(s) 561
Bpu1102I GCTNAGC 1 cut(s) 149
BpuEI CTTGAG 3 cut(s) 119, 264, 303
BsaBI GATNNNNATC 1 cut(s) 461
BsaJI CCNNGG 2 cut(s) 82, 111
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 467, 539
Bse1I ACTGG 4 cut(s) 34, 173, 198, 544
Bse8I GATNNNNATC 1 cut(s) 461
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 2 cut(s) 82, 111
BseJI GATNNNNATC 1 cut(s) 461
BseLI CCNNNNNNNGG 3 cut(s) 364, 467, 539
BseMII CTCAG 2 cut(s) 140, 279
BseNI ACTGG 4 cut(s) 34, 173, 198, 544
BseXI GCAGC 1 cut(s) 561
BseYI CCCAGC 1 cut(s) 421
BsgI GTGCAG 1 cut(s) 222
BshFI GGCC 3 cut(s) 5, 285, 357
BsiHKAI GWGCWC 2 cut(s) 223, 299
BslI CCNNNNNNNGG 3 cut(s) 364, 467, 539
BsnI GGCC 3 cut(s) 5, 285, 357
Bsp1286I GDGCHC 4 cut(s) 135, 223, 299, 319
Bsp143I GATC 3 cut(s) 91, 107, 268
Bsp1720I GCTNAGC 1 cut(s) 149
BspANI GGCC 3 cut(s) 5, 285, 357
BspCNI CTCAG 2 cut(s) 141, 280
BspLI GGNNCC 2 cut(s) 117, 431
BspPI GGATC 2 cut(s) 102, 263
BsrI ACTGG 4 cut(s) 34, 173, 198, 544
BssECI CCNNGG 2 cut(s) 82, 111
BssMI GATC 3 cut(s) 91, 107, 268
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 2 cut(s) 185, 412
Bst6I CTCTTC 1 cut(s) 57
BstDEI CTNAG 2 cut(s) 149, 288
BstKTI GATC 3 cut(s) 94, 110, 271
BstMBI GATC 3 cut(s) 91, 107, 268
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 561
BstX2I RGATCY 1 cut(s) 107
BstXI CCANNNNNNTGG 2 cut(s) 76, 127
BstYI RGATCY 1 cut(s) 107
BsuRI GGCC 3 cut(s) 5, 285, 357
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 3 cut(s) 190, 205, 481
Cfr13I GGNCC 3 cut(s) 79, 115, 284
CviAII CATG 2 cut(s) 173, 259
DdeI CTNAG 2 cut(s) 149, 288
DpnI GATC 3 cut(s) 93, 109, 270
DpnII GATC 3 cut(s) 91, 107, 268
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 82
Eco147I AGGCCT 1 cut(s) 357
Eco24I GRGCYC 3 cut(s) 135, 299, 319
Eco47I GGWCC 2 cut(s) 79, 115
Eco53kI GAGCTC 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 82
EcoT22I ATGCAT 1 cut(s) 334
EcoT38I GRGCYC 3 cut(s) 135, 299, 319
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 2 cut(s) 176, 262
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FatI CATG 2 cut(s) 172, 258
Fnu4HI GCNGC 1 cut(s) 575
FriOI GRGCYC 3 cut(s) 135, 299, 319
Fsp4HI GCNGC 1 cut(s) 575
GluI GCNGC 1 cut(s) 575
GsaI CCCAGC 1 cut(s) 425
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 3 cut(s) 5, 285, 357
Hin1II CATG 2 cut(s) 176, 262
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 1 cut(s) 277
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 1 cut(s) 528
Hpy188I TCNGA 2 cut(s) 276, 363
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 1 cut(s) 528
HpyAV CCTTC 5 cut(s) 24, 306, 368, 383, 420
HpyCH4III ACNGT 2 cut(s) 185, 412
HpyCH4V TGCA 4 cut(s) 203, 332, 494, 577
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 2 cut(s) 149, 288
Hsp92II CATG 2 cut(s) 176, 262
Kzo9I GATC 3 cut(s) 91, 107, 268
LpnPI CCDG 9 cut(s) 47, 98, 125, 154, 211, 300, 407, 472, 525
Lsp1109I GCAGC 1 cut(s) 561
LweI GCATC 3 cut(s) 341, 447, 535
MalI GATC 3 cut(s) 93, 109, 270
MboI GATC 3 cut(s) 91, 107, 268
MboII GAAGA 4 cut(s) 9, 44, 51, 101
MflI RGATCY 1 cut(s) 107
MhlI GDGCHC 4 cut(s) 135, 223, 299, 319
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 42, 72, 229, 302, 373
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 271
MnlI CCTC 4 cut(s) 282, 349, 444, 464
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 334
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 130
NdeII GATC 3 cut(s) 91, 107, 268
NlaIII CATG 2 cut(s) 176, 262
NlaIV GGNNCC 2 cut(s) 117, 431
NsiI ATGCAT 1 cut(s) 334
PceI AGGCCT 1 cut(s) 357
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 1 cut(s) 576
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 111
PspFI CCCAGC 1 cut(s) 421
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 117, 431
PspPI GGNCC 3 cut(s) 79, 115, 284
PsuI RGATCY 1 cut(s) 107
SacI GAGCTC 1 cut(s) 299
SatI GCNGC 1 cut(s) 575
Sau3AI GATC 3 cut(s) 91, 107, 268
Sau96I GGNCC 3 cut(s) 79, 115, 284
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 4 cut(s) 135, 223, 299, 319
SetI ASST 8 cut(s) 16, 100, 126, 150, 164, 299, 450, 491
SfaNI GCATC 3 cut(s) 341, 447, 535
SinI GGWCC 2 cut(s) 79, 115
SmlI CTYRAG 3 cut(s) 134, 279, 318
SmoI CTYRAG 3 cut(s) 134, 279, 318
Sse9I AATT 5 cut(s) 42, 72, 229, 302, 373
SseBI AGGCCT 1 cut(s) 357
SstI GAGCTC 1 cut(s) 299
StuI AGGCCT 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 2 cut(s) 185, 412
TasI AATT 5 cut(s) 42, 72, 229, 302, 373
TscAI CASTG 3 cut(s) 190, 205, 481
TseI GCWGC 1 cut(s) 574
TspDTI ATGAA 6 cut(s) 28, 51, 222, 368, 373, 386
TspRI CASTG 3 cut(s) 190, 205, 481
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 2 cut(s) 79, 115
XapI RAATTY 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.