Rroxscaffold_1G00002760

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000726
Physical Location & Seq
Reverse (-)
3306697 .. 3307630
934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00002760.1

Sequence Viewer

Length: 327 bp
ATGATCGACAATCCCCTCACCGAAAAGCCGGAGCTGAGCTCGAAGCTGCTGGGGAGGGCTGAAGGGTTCTACGCATCAGCTTCACAACAGGAGTTCGGGCTGTTGATGGCCATGAACTTTCATTTTATTCAGGGCAAGTACAACGGGAGCACCATAACTGTGCTGGGGAGGAACCCGGTGTTCAACAAGGTGAGGGAAATGCCGGTGATTGGAGGAAGTGGACTTTTCAGGTTTGCAAGAGGTTATGTTGAAGCAAGAACTCACACATTCACTCCGAGCACCCGAGATGCCATCGTTGAGTACAATGCCTATGTGCTCCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

108

Amino Acids

12.14

Weight (kDa)

9.05

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 1 - 105 7.4e-43 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 2 cut(s) 140, 302
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 2 cut(s) 184, 251
AluBI AGCT 4 cut(s) 34, 39, 46, 80
AluI AGCT 4 cut(s) 34, 39, 46, 80
Alw21I GWGCWC 4 cut(s) 41, 152, 281, 318
Ama87I CYCGRG 1 cut(s) 282
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 1 cut(s) 46
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 3 cut(s) 10, 202, 217
AvaI CYCGRG 1 cut(s) 282
BalI TGGCCA 1 cut(s) 110
BanII GRGCYC 1 cut(s) 41
Bbv12I GWGCWC 4 cut(s) 41, 152, 281, 318
BbvI GCAGC 1 cut(s) 33
BccI CCATC 2 cut(s) 100, 299
BcnI CCSGG 1 cut(s) 176
BisI GCNGC 1 cut(s) 47
BlpI GCTNAGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 48
Bme1390I CCNGG 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 2 cut(s) 83, 277
BplI GAGNNNNNCTC 2 cut(s) 23, 55
Bpu1102I GCTNAGC 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse118I RCCGGY 1 cut(s) 202
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMII CTCAG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 33
BseYI CCCAGC 2 cut(s) 49, 163
BshFI GGCC 1 cut(s) 110
BsiHKAI GWGCWC 4 cut(s) 41, 152, 281, 318
BsiHKCI CYCGRG 1 cut(s) 282
BsiSI CCGG 3 cut(s) 29, 176, 203
BslI CCNNNNNNNGG 1 cut(s) 209
BsnI GGCC 1 cut(s) 110
BsoBI CYCGRG 1 cut(s) 282
Bsp1286I GDGCHC 4 cut(s) 41, 152, 281, 318
Bsp143I GATC 1 cut(s) 3
Bsp1720I GCTNAGC 1 cut(s) 35
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 202
BssAI RCCGGY 1 cut(s) 202
BssMI GATC 1 cut(s) 3
Bst4CI ACNGT 1 cut(s) 160
BstDEI CTNAG 1 cut(s) 35
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstSCI CCNGG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 33
BsuRI GGCC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 202
Csp6I GTAC 2 cut(s) 139, 301
CviAII CATG 1 cut(s) 112
CviJI RGCY 8 cut(s) 28, 34, 39, 46, 59, 80, 100, 110
CviKI_1 RGCY 8 cut(s) 28, 34, 39, 46, 59, 80, 100, 110
CviQI GTAC 2 cut(s) 139, 301
DdeI CTNAG 1 cut(s) 35
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
EaeI YGGCCR 1 cut(s) 108
Ecl136II GAGCTC 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 39
Eco57I CTGAAG 1 cut(s) 81
Eco88I CYCGRG 1 cut(s) 282
EcoICRI GAGCTC 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 41
FaeI CATG 1 cut(s) 115
FaiI YATR 4 cut(s) 113, 155, 246, 312
FatI CATG 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 47
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 1 cut(s) 47
GluI GCNGC 1 cut(s) 47
GsaI CCCAGC 2 cut(s) 53, 167
HaeIII GGCC 1 cut(s) 110
HapII CCGG 3 cut(s) 29, 176, 203
Hin1II CATG 1 cut(s) 115
HpaII CCGG 3 cut(s) 29, 176, 203
HphI GGTGA 3 cut(s) 10, 202, 217
Hpy166II GTNNAC 1 cut(s) 221
Hpy188I TCNGA 1 cut(s) 276
Hpy8I GTNNAC 1 cut(s) 221
HpyAV CCTTC 1 cut(s) 56
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 1 cut(s) 236
HpyF3I CTNAG 1 cut(s) 35
Hsp92II CATG 1 cut(s) 115
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 3 cut(s) 31, 147, 321
LpnPI CCDG 8 cut(s) 35, 42, 74, 116, 149, 189, 214, 216
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 2 cut(s) 83, 277
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MhlI GDGCHC 4 cut(s) 41, 152, 281, 318
MlsI TGGCCA 1 cut(s) 110
MluNI TGGCCA 1 cut(s) 110
MnlI CCTC 6 cut(s) 26, 48, 162, 186, 206, 233
Mox20I TGGCCA 1 cut(s) 110
MscI TGGCCA 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 158
Msp20I TGGCCA 1 cut(s) 110
MspI CCGG 3 cut(s) 29, 176, 203
MspR9I CCNGG 1 cut(s) 176
NciI CCSGG 1 cut(s) 176
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 1 cut(s) 173
PkrI GCNGC 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 41
PspFI CCCAGC 2 cut(s) 49, 163
PspN4I GGNNCC 1 cut(s) 173
RsaI GTAC 2 cut(s) 140, 302
RsaNI GTAC 2 cut(s) 139, 301
RseI CAYNNNNRTG 1 cut(s) 158
SacI GAGCTC 1 cut(s) 41
SatI GCNGC 1 cut(s) 47
Sau3AI GATC 1 cut(s) 3
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 4 cut(s) 41, 152, 281, 318
SetI ASST 7 cut(s) 36, 41, 48, 82, 192, 233, 244
SfaNI GCATC 2 cut(s) 83, 277
SmiMI CAYNNNNRTG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 2 cut(s) 6, 41
TatI WGTACW 2 cut(s) 138, 300
TseI GCWGC 1 cut(s) 46
TspDTI ATGAA 2 cut(s) 110, 128
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.